Growth Response of Avena sativa in Amino-Acids-Rich Soils Converted from Phenol-Contaminated Soils by Corynebacterium glutamicum

2012 ◽  
Vol 22 (4) ◽  
pp. 541-546 ◽  
Author(s):  
Soo Youn Lee
Keyword(s):  
Amino Acids ◽  
Corynebacterium Glutamicum ◽  
Avena Sativa ◽  
Contaminated Soils ◽  
Growth Response

scholarly journals The Content of Non-essential Amino Acids in the Grains of Winter and Spring Varieties of Oats (Avena sativa L.) under the Conditions of Central Southern Bulgaria

2013 ◽  
Vol 14 (1) ◽  
pp. 105
Author(s):  
T. Georgieva ◽  
P. Zorovski
Keyword(s):  
Amino Acids ◽  
Glutamic Acid ◽  
G Protein ◽  
Aspartic Acid ◽  
Avena Sativa ◽  
Essential Amino Acids

The purpose of this survey is to study the content of non-essential amino acids in four winter (Dunav 1, Ruse 8, Resor 1, Line M-K) and five spring (Obraztsov chiflik 4, Mina, HiFi, Novosadski golozarnest and Prista 2) cultivars of oats grown in Central Southern Bulgaria within the period from 2007 to 2009. The tested cultivars have different contents of non-essential amino acids. Dunav 1 has the highest quantity of glicine (5.12 g/100 g protein) of all the winter cultivars, Ruse 8 has the highest quantity of alanine (5.69 g/100 g protein) and Resor 1 – the highest quantity of arginine (6.14 g/100 g protein). Generally speaking, the spring cultivars have a larger quantity of glutamic acid (from 25.86 to 26.07 g/100 g protein) and proline (from 6.15 to 8.21 g/100 g protein) but a smaller quantity of glycine (from 4.68 to 4.99 g/100 g protein) compared to the winter cultivars. The naked cultivar Mina has the highest quantity of cystine (2.14 g/100 g protein), cultivar Prista 2 has the highest quantity of proline (8.21 g/100 g protein) and glutamic acid (26.07 g/100g protein) and HiFi ranks first in terms of aspartic acid (9.05 g/100 g protein), serine (5.02 g/100 g protein) and tyrosine (2.09 g/100 g protein). In the study we have also established certain relations between non-essential amino acids.

Chromopeptides from Phytochrome and Phycocyanin. NM R Studies o f the Pfr and Pr Chromophore of Phytochrome and E,Z Isomeric Chromophores of Phycocyanin

1983 ◽  
Vol 38 (5-6) ◽  
pp. 359-368 ◽  
Author(s):  
Fritz Thümmler ◽  
Wolfhart Rüdiger ◽  
Edmund Cmiel ◽  
Siegfried Schneider
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Avena Sativa ◽  
Maximum Amount ◽  
Acid Interaction ◽  
Spirulina Maxima ◽  
Nmr Spectrum ◽  
Pepsin Digestion ◽  
H Nmr ◽  
Amino Acid Interaction

Chromopeptides were prepared by pepsin digestion of C-phycocyanin isolated from the cyano­bacterium Spirulina maxima and of phytochrome isolated from seedlings of Avena sativa L. The chromopeptides were characterized by amino acid analysis. The ZZZ configurated chromophore of the phycocyanin peptide was transformed into its ZZE configurated isomer by the method of Falk et al. (Mh. Chemie 111, 159- 175, 1980) which had previously been applied to biliverdins. The 500 MHz 1HNMR spectrum of the ZZE configurated chromopeptides confirmed that its chromophore has the 15 E configuration. Irradiation yielded the ZZZ configurated isomer for which the 1H NMR spectrum was also recorded. Native phytochrome was irradiated at 660 nm to yield the maximum amount of the Pfr from (about 75% of total phytochrome). By digestion in the dark the previously described Pfr chromopeptide was obtained. The 500 MHz 1H NMR spectrum was compared with that of the ZZE phycocyanin peptide. It confirmed the 15 E con­figuration of the Pfr chromopeptide. Irradiation yielded the 15 Z configurated Pr chromopeptide. Comparison of the high resolution 1HNMR spectra of Pfr and Pr chromopeptides revealed that not only the chromophore resonances but also those of some amino acids are changed by the Pfr → Pr chromopeptide phototransformation. The results are discussed in terms of chromophore amino acid interaction.

Coproduction of cell-bound and secreted value-added compounds: Simultaneous production of carotenoids and amino acids by Corynebacterium glutamicum

2018 ◽  
Vol 247 ◽  
pp. 744-752 ◽  
Author(s):  
Nadja A. Henke ◽  
Daniela Wiebe ◽  
Fernando Pérez-García ◽  
Petra Peters-Wendisch ◽  
Volker F. Wendisch
Keyword(s):  
Amino Acids ◽  
Corynebacterium Glutamicum ◽  
Value Added ◽  
Simultaneous Production

scholarly journals Response of Avena sativa L. and the soil microbiota to the contamination of soil with Shell diesel oil

2018 ◽  
Vol 64 (No. 3) ◽  
pp. 102-107 ◽  
Author(s):  
Borowik Agata ◽  
Wyszkowska Jadwiga
Keyword(s):  
Avena Sativa ◽  
Contaminated Soils ◽  
Dry Matter ◽  
Rapid Development ◽  
Chemical Properties ◽  
Diesel Oil ◽  
Barley Straw ◽  
Physico Chemical ◽  
Organotrophic Bacteria ◽  
Nutritional Needs

This study analysed the changes in the development of Avena sativa L. cultivated on soil contaminated with diesel oil (DO; 0, 7, 14 and 21 mL/kg of soil dry matter), and in the microbiological, biochemical, chemical and physico-chemical properties of the soil. In addition to basic fertilisation fulfilling the nutritional needs of the oat plant, finely ground barley straw and finely ground charcoal was also applied. The study revealed a highly toxic effect of DO on the growth and development of Avena sativa L. The uptake of macro- and micronutrients by the tested plant decreased significantly. The active bacteria were identified based on the analysis of 16S rRNA coding sequences. In objects contaminated with DO, a more rapid development of organotrophic bacteria, actinomyces and fungi was observed, as well as higher activity of dehydrogenases, catalase, urease, acid phosphatase, alkaline phosphatase and β-glucosidase. In the soils contaminated with DO, more C<sub>org</sub> and available and exchangeable potassium were found than in the non-contaminated soils. DO did not have such a significant effect on the contents of other elements in the soil. The use of charcoal and straw stimulated both the development of microorganisms and the activity of soil enzymes, yet it did not mitigate the adverse effect of DO on the growth or development of the oat plant.

Digestibilities of nitrogen and amino acids in soya-bean, sunflower, meat and rapeseed meals measured with pigs and poultry

1989 ◽  
Vol 48 (1) ◽  
pp. 157-179 ◽  
Author(s):  
S. Green ◽  
T. Kiener
Keyword(s):  
Amino Acids ◽  
Growth Response ◽  
Live Weight ◽  
Rapeseed Meal ◽  
Soya Bean ◽  
Growing Pigs ◽  
Sunflower Meal ◽  
Meat Meal ◽  
Bean Meal ◽  
Soya Bean Meal

ABSTRACTIn order to determine the relative digestibilities of nitrogen and amino acids in foodstuffs for pigs and poultry, and the effects of manufacturing methods, equal quantities of soya-bean meal, sunflower meals [hulled (sunflower meal 1) and dehulled (sunflower meal 2)], meat meals [made with (meat meal 1), and without (meat meal 2), blood added at 250 g/kg meat tissue (wet weights)] and rapeseed meals [seeds heated at 80°C (rapeseed meal 1) or 100°C (rapeseed meal 2)] were mixed with protein-free ingredients. The diets were given to five growing pigs with ileo-rectal anastomoses, and, by crop-intubation, to 12 caecectomized and 12 intact cocks. Excreta were collected over 48-h periods. Endogenous excretion was estimated by giving protein-free diets.In the order, soya-bean meal, sunflower meals 1, and 2, meat meals 1, and 2, rapeseed meals 1, and 2, true digestibilities were: with pigs, of nitrogen, 0·81, 0·80, 0·79, 0·64, 0·79, 0·73, 0·70 (s.e.d. 0·030), of lysine, 0·84, 0·83, 0·84, 0·65, 0·84, 0·76, 0·72 (s.e.d. 0·032); with caecectomized birds, of nitrogen, 0·92, 0·91, 0·91, 0·66, 0·78, 0·74, 0·75 (s.e.d. 0·018), of lysine 0·92, 0·91, 0·93, 0·62, 0·79, 0·70, 0·70 (s.e.d. 0·020); with intact birds, values were similar to those with caecectomized birds for soya-bean, and the sunflower meals, but lesser for meat meals 1 and 2; the solubilities of nitrogen in pepsin were 0·96, 0·92, 0·93, 0·80, 0·89, 0·87, 0·87.Two hundred and eighty pigs (initial live weights 10 kg) were used to compare growth response to free lysine with that to lysine in soya-bean meal and sunflower meal 2. Lysine availabilities, assessed by analyses of regressions of live-weight gain against lysine intake were 0·82 (s.e. 0·12) for soya-bean meal, and 0·82 (s.e. 0·18) for sunflower meal 2.Amino acid digestibilities of the sunflower meals were similar to those of soya-bean meal, and were not influenced by dehulling; values for the rapeseed meals were lower, and unaffected by differences in heating severity; values for the meat meal were reduced by blood addition. Values differed between pigs and poultry, but there was consistency in the extent to which each species discriminated between some foodstuffs. The pepsin test was insensitive. The large standard errors associated with availability values prevented meaningful comparisons with digestibility values.

scholarly journals Anaerobic Growth of Corynebacterium glutamicum via Mixed-Acid Fermentation

2015 ◽  
Vol 81 (21) ◽  
pp. 7496-7508 ◽  
Author(s):  
Andrea Michel ◽  
Abigail Koch-Koerfges ◽  
Karin Krumbach ◽  
Melanie Brocker ◽  
Michael Bott
Keyword(s):  
Amino Acids ◽  
Corynebacterium Glutamicum ◽  
Tricarboxylic Acid Cycle ◽  
Model Organism ◽  
Anaerobic Growth ◽  
Efficient Production ◽  
Ph Homeostasis ◽  
Anaerobic Conditions ◽  
Acid Fermentation ◽  
Content Type

ABSTRACTCorynebacterium glutamicum, a model organism in microbial biotechnology, is known to metabolize glucose under oxygen-deprived conditions tol-lactate, succinate, and acetate without significant growth. This property is exploited for efficient production of lactate and succinate. Our detailed analysis revealed that marginal growth takes place under anaerobic conditions with glucose, fructose, sucrose, or ribose as a carbon and energy source but not with gluconate, pyruvate, lactate, propionate, or acetate. Supplementation of glucose minimal medium with tryptone strongly enhanced growth up to a final optical density at 600 nm (OD600) of 12, whereas tryptone alone did not allow growth. Amino acids with a high ATP demand for biosynthesis and amino acids of the glutamate family were particularly important for growth stimulation, indicating ATP limitation and a restricted carbon flux into the oxidative tricarboxylic acid cycle toward 2-oxoglutarate. Anaerobic cultivation in a bioreactor with constant nitrogen flushing disclosed that CO2is required to achieve maximal growth and that the pH tolerance is reduced compared to that under aerobic conditions, reflecting a decreased capability for pH homeostasis. Continued growth under anaerobic conditions indicated the absence of an oxygen-requiring reaction that is essential for biomass formation. The results provide an improved understanding of the physiology ofC. glutamicumunder anaerobic conditions.

INHIBITION OF BOVINE GROWTH HORMONE ACTIVITY BY ACETYL PROTEINS

1966 ◽  
Vol 35 (1) ◽  
pp. 45-52
Author(s):  
M. SONENBERG ◽  
W. L. MONEY
Keyword(s):  
Amino Acids ◽  
Growth Hormone ◽  
Bovine Serum Albumin ◽  
Acetic Anhydride ◽  
Bovine Serum ◽  
Growth Response ◽  
Bovine Growth Hormone ◽  
Optimal Conditions ◽  
Hormone Activity ◽  
Intact Rats

SUMMARY Bovine growth hormone (BGH) was acetylated with acetic anhydride under a variety of conditions and the optimal conditions defined to obtain a growth hormone inhibitor. Acetyl BGH as well as acetyl thyrotrophin, acetyl bovine serum albumin and acetyl lactalbumin in equivalent doses inhibited the growth response of hypophysectomized and intact rats to unmodified bovine growth hormone. N-acetyl dl-proline and O-acetyl dl-serine of many amino and hydroxyl acetyl amino acids also inhibited BGH but in a higher molar dose than that of the acetyl proteins.

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