MATTERS NEEDING ATTENTION FOR APPLYING THE QUARTZ CRYSTAL MICROBALANCE TECHNIQUE TO DETECT THE CELL MORPHOLOGY

2009 ◽  
Vol 21 (06) ◽  
pp. 415-420 ◽  
Author(s):  
Hung-Che Chou ◽  
Tsong-Rong Yan ◽  
Chao-Fa Lee
Keyword(s):  
Experimental Data ◽  
Cell Culture ◽  
System Design ◽  
Quartz Crystal Microbalance ◽  
Quartz Crystal ◽  
Important Application ◽  
The Other ◽  
Cell Detection ◽  
A Cell ◽  
Culture Environment

Using quartz crystal microbalance (QCM) to design a cell detection platform is an important application of QCM. In the process of system design and application, there still have some points, which are easily overlooked or are not understood by researchers. Most of the problems can be separated into three types. One is the cell culture environment conditions that affect the system signals, another is the system unification or lack of communication over regulations, and the other is the over-analysis of the experimental data.

scholarly journals Quartz Crystal Microbalance as a Sensor to Characterize Macromolecular Assembly Dynamics

2009 ◽  
Vol 2009 ◽  
pp. 1-17 ◽  
Author(s):  
K. Kanazawa ◽  
Nam-Joon Cho
Keyword(s):  
Cell Membrane ◽  
Quartz Crystal Microbalance ◽  
Quartz Crystal ◽  
Analytical Techniques ◽  
Top Down ◽  
Macromolecular Assembly ◽  
Basic Measurement ◽  
Quartz Crystal Microbalance Sensor ◽  
A Cell ◽  
Peptide Interaction

The quartz crystal microbalance sensor has a resonant frequency and a quality factor which can be used to probe the properties of nanometer thick film loads. A recent review by Arnau (2008) has discussed many of the considerations necessary to accurately probe for these properties. To avoid needless duplication but to still provide an adequate background for the new user, we briefly outline the basic measurement methodologies and analytical techniques that were covered in the review. Details will be provided on some specific perspectives of the authors. For example, the special precautions necessary when dealing with soft films (polymeric and biological) under liquid are overviewed. To illustrate applications of the QCM technique, simple bilayer and vesicle behaviors are discussed, along with the structural transformation resulting from protein adsorption onto an intact vesicle adlayer. The amphipathic -helical (AH) peptide interaction is given as a particular example. Lastly, we summarize a top-down approach to functionalize a surface with a cell membrane and to study its interaction with proteins.

scholarly journals The Bioactivated Interfacial Behavior of the Fluoridated Hydroxyapatite-Coated Mg-Zn Alloy in Cell Culture Environments

2011 ◽  
Vol 2011 ◽  
pp. 1-7 ◽  
Author(s):  
Jianan Li ◽  
Lei Cao ◽  
Yang Song ◽  
Shaoxiang Zhang ◽  
Changli Zhao ◽  
...  
Keyword(s):  
Cell Culture ◽  
Mg Alloys ◽  
Specific Cell ◽  
Apatite Formation ◽  
Interfacial Behavior ◽  
Before And After ◽  
A Cell ◽  
Culture Environment ◽  
Thermodynamic Mechanism ◽  
Zn Alloy

A partially fluorine substituted hydroxyapatite- (FHA-) coated Mg-Zn alloy was prepared to investigate the interfacial behavior of degradable Mg-based biomaterials with degradable bioactive coatings in a cell culture environment. Peaks from the results of X-ray diffraction (XRD) were characterized and compared before and after cell culture. It was found that Ca-P, including poorly crystalline ion-substituted Ca-deficient HA (CDHA), was formed in greater amounts on the interface of coated samples compared with the uncoated ones. A thermodynamic mechanism for Ca-P formation on biodegradable Mg alloys in a cell culture environment is proposed. Combined with improved cell calcification, the-FHA coated Mg alloys have the ability to promote CDHA formation, as expected thermodynamically. It is suggested that the specific cell culture environment and the bone-like FHA coatings together facilitate the observed behavior. Moreover, cell culture environment probably increased the biomineralization to a detectable level by affecting the kinetics of apatite formation.

scholarly journals Protein Adsorption to Titanium and Zirconia Using a Quartz Crystal Microbalance Method

2017 ◽  
Vol 2017 ◽  
pp. 1-8 ◽  
Author(s):  
You Kusakawa ◽  
Eiji Yoshida ◽  
Tohru Hayakawa
Keyword(s):  
Surface Roughness ◽  
Protein Adsorption ◽  
Quartz Crystal Microbalance ◽  
Quartz Crystal ◽  
Adsorption Rate ◽  
Force Microscopy ◽  
Atomic Force ◽  
A Cell ◽  
Addition Amount

Protein adsorption onto titanium (Ti) or zirconia (ZrO2) was evaluated using a 27 MHz quartz crystal microbalance (QCM). As proteins, fibronectin (Fn), a cell adhesive protein, and albumin (Alb), a cell adhesion-inhibiting protein, were evaluated. The Ti and ZrO2 sensors for QCM were characterized by atomic force microscopy and electron probe microanalysis observation, measurement of contact angle against water, and surface roughness. The amounts of Fn and Alb adsorbed onto the Ti and ZrO2 sensors and apparent reaction rate were obtained using QCM measurements. Ti sensor showed greater adsorption of Fn and Alb than the ZrO2 sensor. In addition, amount of Fn adsorbed onto the Ti or ZrO2 sensors was higher than that of Alb. The surface roughness and hydrophilicity of Ti or ZrO2 may influence the adsorption of Fn or Alb. With regard to the adsorption rate, Alb adsorbed more rapidly than Fn onto Ti. Comparing Ti and ZrO2, Alb adsorption rate to Ti was faster than that to ZrO2. Fn adsorption will be effective for cell activities, but Alb adsorption will not. QCM method could simulate in vivo Fn and Alb adsorption to Ti or ZrO2.

scholarly journals Asymmetry of nanoparticle inheritance upon cell division: Effect on the coefficient of variation

PLoS ONE ◽  
2020 ◽  
Vol 15 (11) ◽  
pp. e0242547
Author(s):  
Tim Lijster ◽  
Christoffer Åberg
Keyword(s):  
Experimental Data ◽  
Cell Population ◽  
High Throughput ◽  
Coefficient Of Variation ◽  
Daughter Cell ◽  
The Other ◽  
Experimental Assessment ◽  
Daughter Cells ◽  
A Cell ◽  
Characteristic Evolution

Several previous studies have shown that when a cell that has taken up nanoparticles divides, the nanoparticles are inherited by the two daughter cells in an asymmetrical fashion, with one daughter cell receiving more nanoparticles than the other. This interesting observation is typically demonstrated either indirectly using mathematical modelling of high-throughput experimental data or more directly by imaging individual cells as they divide. Here we suggest that measurements of the coefficient of variation (standard deviation over mean) of the number of nanoparticles per cell over the cell population is another means of assessing the degree of asymmetry. Using simulations of an evolving cell population, we show that the coefficient of variation is sensitive to the degree of asymmetry and note its characteristic evolution in time. As the coefficient of variation is readily measurable using high-throughput techniques, this should allow a more rapid experimental assessment of the degree of asymmetry.

Manufacturing and Control of a Robotic Device for Time-averaged Simulated Micro and Partial Gravity of a Cell Culture Environment

2019 ◽  
Vol 18 (1) ◽  
pp. 53-64
Author(s):  
Yoon Jae Kim ◽  
Min Hyuk Lim ◽  
Byoungjun Jeon ◽  
Dong Hyun Choi ◽  
Haeri Lee ◽  
...  
Keyword(s):  
Cell Culture ◽  
Robotic Device ◽  
Partial Gravity ◽  
A Cell ◽  
Culture Environment ◽  
And Control

Quartz crystal microbalance biosensor for label-free MDA MB 231 cancer cell detection via notch-4 receptor

Talanta ◽  
2019 ◽  
Vol 204 ◽  
pp. 840-845 ◽  
Author(s):  
Monireh Bakhshpour ◽  
Ayse Kevser Piskin ◽  
Handan Yavuz ◽  
Adil Denizli
Keyword(s):  
Cancer Cell ◽  
Quartz Crystal Microbalance ◽  
Quartz Crystal ◽  
Cell Detection ◽  
Label Free ◽  
Cancer Cell Detection

New Groove Structures for Miniature Quartz Crystal Microbalance with Low Crystal Impedance

2012 ◽  
Vol 538-541 ◽  
pp. 2461-2465
Author(s):  
Zong Han Liu ◽  
Chih Hsiung Shen
Keyword(s):  
Quartz Crystal Microbalance ◽  
Quartz Crystal ◽  
Mechanical Response ◽  
Piezoelectric Effect ◽  
Effective Area ◽  
The Other ◽  
Q Value ◽  
Innovative Design ◽  
Groove Structure ◽  
Piezoelectric Performance

Throughout the world, there are numerous of studies on quartz crystal microbalance (QCM). Today, we come up with a new way to reduce both the size of QCM structure and the crystal impedance without lowering the sensitivity. Beyond the conventional design of QCM originally developed in recent years, a new disk shape with multi-groove structures is proposed and investigated thoroughly to realize the miniaturized QCM. Along the miniaturization stage, there are two main approaches to remain the efficiency of vibration. One is to improve the piezoelectric effect which can be further increased by reducing the distance of electric field and the other is to improve the effective area of electric potential flow between the electrodes. Several proposed design with grooves in the disk has been proposed to make effective area larger, crystal impedance lower ,and Q value higher. We will investigate between different groove structures on QCM under the same bias voltage. In this study, we propose and verify the innovative design of QCM and a thorough investigation of the electric and mechanical response were performed with evidence marvelous efficiency. Consequently, the three groove structure gives the best piezoelectric performance than the other.

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