scholarly journals Aerobic stability in corn silage (Zea maysL.) ensiled with different microbial additives

2017 ◽  
Vol 39 (4) ◽  
pp. 357 ◽  
Author(s):  
Valter Harry Bumbieris Junior ◽  
Vinícius André De Pietro Guimarães ◽  
Ana Paula De Souza Fortaleza ◽  
Fernando Luiz Massaro Junior ◽  
Gabriella Jorgette de Moraes ◽  
...  
Keyword(s):  
Zea Mays ◽  
Bacillus Subtilis ◽  
Experimental Design ◽  
Lactobacillus Plantarum ◽  
Corn Silage ◽  
Control Treatment ◽  
Propionibacterium Acidipropionici ◽  
Aerobic Stability ◽  
Corn Hybrid ◽  
Plot Design

  This study aimed to assess different microbial additives, regarding the efficiency of aerobic stability in corn silages. The corn hybrid used for silages production was the DKB 310. The treatments consisted of: 1) control treatment without any microbial additive; 2) Treatment with LPPA composed of: Lactobacillus plantarum CCT 0580 3.1 x 1010 CFU g-1 and Propionibacterium acidipropionici CCT 4843 3.1 x 1010 CFU g-1; 3) Treatment with Inoculum, composed of Bacillus subtilis CCT 0089 3.0 x 109 CFU g-1, Lactobacillus plantarum CCT 0580 1.2 x 1010 CFU g-1 and Propionibacterium acidipropionici CCT 4843 1.5 x 1010 CFU g-1 and 4) Treatment LB, composed only of Lactobacillus buchneri CCT 3746 2.6 x 1010 CFU g-1. The experimental design was completely randomized with five replicates for each treatment. For the parameters evaluated daily, we used the split plot design, in which the different silages were assigned to the plots and the time of exposure to air was assigned to the subplots. There was no difference of additives on silage pH in any of the evaluation days. The control silage reached higher temperatures indicating greater vulnerability. All additives had no aerobic instability. Silages made with the use of microbial additives were effective in maintaining the aerobic stability. 

scholarly journals Changes in the nutritive value and aerobic stability of corn silages inoculated with Bacillus subtilis alone or combined with Lactobacillus plantarum

2016 ◽  
Vol 56 (11) ◽  
pp. 1867 ◽  
Author(s):  
Erika C. Lara ◽  
Fernanda C. Basso ◽  
Flávia B. de Assis ◽  
Fernando A. Souza ◽  
Telma T. Berchielli ◽  
...  
Keyword(s):  
Organic Matter ◽  
Bacillus Subtilis ◽  
Chemical Composition ◽  
Lactobacillus Plantarum ◽  
Corn Silage ◽  
In Vitro Digestibility ◽  
Aerobic Stability ◽  
Organic Matter Digestibility ◽  
Corn Plants

Chemical composition, fermentation characteristics, in vitro digestibility and aerobic stability were evaluated in corn silage inoculated with microbial additives in two different experiments. Inoculant treatments (untreated, Bacillus subtilis and B. subtilis combined with Lactobacillus plantarum) were applied to fresh forages. Chopped corn plants (2B655 Hx) were ensiled in laboratory silos for periods of 7, 14, 21 and 63 days to evaluate the fermentation parameters. The experimental silos were weighed to determine gas losses. After the ensiling period, the silage was sampled to determine chemical composition and in vitro organic matter digestibility. To evaluate aerobic stability, chopped corn plants (AG‐1051) were ensiled in laboratory silos that were opened after 96 days of ensiling. The silage was placed in different buckets containing data loggers. The silage was sampled after 0, 4, 8 and 12 days of exposure to air to evaluate the microbial populations and pH. The data were analysed as a completely randomised design using a mixed repeated-measures model in the MIXED procedure of SAS. To evaluate each treatment relative to the fermentation times, a regression analysis using the PROC REG procedure of SAS was applied. A significance level of P < 0.05 was used. Inoculation with both strains increased lactic acid concentration, whereas the use of B. subtilis alone or combined with L. plantarum improved in vitro apparent organic matter digestibility. In the B. subtilis and B. subtilis combined with L. plantarum silages, moulds and yeasts decreased, and aerobic stability was improved. Inoculation with B. subtilis alone or combined with L. plantarum improved the nutritional value and aerobic stability of corn silage.

scholarly journals SILAGE QUALITY OF CORN AND SORGHUM ADDED WITH FORAGE PEANUTS

2016 ◽  
Vol 29 (2) ◽  
pp. 465-472 ◽  
Author(s):  
WALKÍRIA GUIMARÃES CARVALHO ◽  
KÁTIA APARECIDA DE PINHO COSTA ◽  
PATRÍCIA SOARES EPIFANIO ◽  
ROZANA CASTRO PERIM ◽  
DANIEL AUGUSTO ALVES TEIXEIRA ◽  
...  
Keyword(s):  
Zea Mays ◽  
Experimental Design ◽  
Sorghum Bicolor ◽  
Crude Protein ◽  
Corn Silage ◽  
Efficient Technique ◽  
Arachis Pintoi ◽  
Silage Quality ◽  
Sorghum Silage

ABSTRACT: Corn and sorghum are standard silage crops because of their fermentative characteristics. While corn and sorghum silages have lower crude protein (CP) contents than other crops, intercropping with legumes can increase CP content. Furthermore, one way to increase CP content is the addition of legumes to silage. Consequently, the research objective was to evaluate the fermentative and bromatological characteristics of corn (Zea mays) and sorghum (Sorghum bicolor) silages added with forage peanuts (Arachis pintoi). The experimental design was completely randomized with four replicates. The treatments consisted of corn silage, sorghum silage, forage peanut silage, corn silage with 30% forage peanut, and sorghum silage with 30% forage peanut. The results showed that the corn and sorghum added with peanut helped to improve the silage fermentative and bromatological characteristics, proving to be an efficient technique for silage quality. The forage peanut silage had lower fermentative characteristics than the corn and sorghum silages. However, the forage peanut silage had a greater CP content, which increased the protein contents of the corn and sorghum silages when intercropped with forage peanuts.

scholarly journals The effect silage aditives supplementation on dynamic fermentation process, quality and aerobic stability of corn silage

2008 ◽  
Vol 56 (1) ◽  
pp. 157-164
Author(s):  
Václav Pyrochta ◽  
Libor Kalhotka ◽  
Petr Doležal
Keyword(s):  
Acetic Acid ◽  
Lactobacillus Plantarum ◽  
Acid Content ◽  
Corn Silage ◽  
Bacterial Strains ◽  
Total Acid ◽  
Bacterial Inoculants ◽  
Aerobic Stability ◽  
Fermentation Quality

In the experiment, the effect of additives supplementation on the fermentation quality of corn silage was examined, compared with the untreated control (K). The aditive „A“ contained bacterial component of (Propionibactrium acidipropionici – MA126/4U 3*1010 and Lactobacillus plantarum – MA18/5U). The effective substances of bacterial inoculants „B“, selected were bacterial strains of (Lactobacillus casei ssp. rhamnosus LC – 705 DSM 7061 4*1011, Propionibacterium freudenreichii spp. shermanii JS DSM 6067 2-4*1011). There were used as effective substances of bacterial inoculants „C“ lactic bacteria and enzyme (Lactobacillus plantarum CCM 3769 1.67*1010, Lactococcus lactis CCM 4754 1.67*1010, Enterococcus faecium CCM 6226 1.67*1010, Pediococcus pentosaceus CCM 3770 1,67*1010, cellulase, hemicellulase, sodium benzoate). They were applied in the dose of prescript by producer. At conservations with all aditivum were statistically significant (P < 0.01) increase of lactic acid formation from 55.31±9.72 g/kg DM of control silage to 59.60±10.84 g/kg DM aditivum „A“, 59.36±10.04 g/ kg DM aditivum „B“ rather to 60.74±9.90 g/kg DM aditivum „C“. Aditives „A“ and „B“ were statistically significant (P < 0.01) increase propoinic acid and total fermentation acid content in silages occured. The fermentation characteristics in the microbial aditivum silages by us were more favourable. The date of fermentation was statistically significant (P < 0.01) increase the contents of acetic acid from 45.49±2.83 g/kg DM of 4st day to 63.07±4.25 g/kg DM of 32ndday rather to 67.70±2.94 g/kg DM of 64st day. There were statistically significant (P < 0.01) increase contents of acetic acid and total acid content. The date of fermentation was statistically significant (P < 0.01) degressive of pH.

scholarly journals Effect of 11CFT and 11C33 inoculants on the chemical and fermentation composition, and aerobic stability of corn silage during the feed out period

2021 ◽  
pp. 395-410
Author(s):  
Tânia Mara Becher Ribas ◽  
◽  
Mikael Neumann ◽  
Egon Henrique Horst ◽  
Fernando Braga Cristo ◽  
...  
Keyword(s):  
Acetic Acid ◽  
Lactic Acid ◽  
Experimental Design ◽  
Lactic Acid Bacteria ◽  
Propionic Acid ◽  
Dry Matter ◽  
Corn Silage ◽  
Bacterial Inoculants ◽  
Aerobic Stability

The objective was to evaluate the efficiency of two bacterial inoculants, 11CFT and 11C33, with different genera of lactic acid bacteria on the chemical and fermentation composition of the silage, and the temperature and pH behavior of the silage during the feed out period. The experimental design used was randomized blocks, with three treatments: corn silage without inoculant (control); corn silage with 11CFT inoculant (consisting of strains of Lactobacillus buchneri and L. casei); and corn silage with 11C33 inoculant (consisting of strains of L. buchneri, L. plantarum and Enterococcus faecium). The use of both inoculants increased the concentration of lactic acid in the silage (22.42 g kg-1 for control against 36.00 and 33.33 g kg-1 for 11CFT and 11C33, respectively) and reduced aerobic dry matter losses. The silage treated with 11C33 obtained a higher concentration of acetic acid (17.44 g kg-1) and propionic acid (2.08 g kg-1). The 11CFT inoculant provided a lower concentration of ethanol, however, without differing from the silage with 11C33 (0.70 and 1.61 g kg-1, respectively). Even without variations in temperature and pH at silage unloading, the use of the 11C33 inoculant generated a higher concentration of acetic and propionic acid, providing better aerobic stability days after unloading. Both inoculants also improved the in situ ruminal digestibility of corn silage compared to control silage. They provide an increase in the content of lactic and propionic acids, which assist to reduce dry matter losses and ethanol production. There were no variations in temperature and pH at the silo unloading, however, the use of the 11C33 inoculant generated a higher concentration of acetic and propionic acids providing better aerobic stability after exposure to air.

scholarly journals Fermentation Profile, Nutritional Value, and Microbial Population of C4 Grasses Silages with or Without Bacterial Additive

2021 ◽  
Author(s):  
Sarah E. E. Bernardo ◽  
Paulo H. B. Chrisostomo ◽  
Michele G. Camilo ◽  
Danielle F. Baffa ◽  
Elizabeth F. Processi ◽  
...  
Keyword(s):  
Lactobacillus Plantarum ◽  
Pearl Millet ◽  
Nutritional Value ◽  
Microbial Population ◽  
Block Design ◽  
Gas Production ◽  
Propionibacterium Acidipropionici ◽  
Aerobic Stability ◽  
Fermentation Profile

Abstract The objective of the present study was to evaluate the use of bacterial additive (Lactobacillus plantarum and Propionibacterium acidipropionici) on chemical composition, in vitro gas production, pH, losses, aerobic stability, and microbial population of corn, pearl millet, and sorghum silages in plastic bags silos (without vacuum). The experiment was carried out in a randomized block design, in a 2 × 3 factorial scheme, with or without additive ([Control] without additive and Lactobacillus plantarum [2.5 × 1010 cfu/g] and Propionibacterium acidipropionici [2.5 × 1010 cfu/g] Biomax corn, Lallemand, Saint-Simon, France [LP]) and three crops of agricultural interest; pearl millet, sorghum, and corn, with four replicates per treatment. We performed chemical analyses and in vitro gas production to determine the nutritional value of the silages. We also evaluated the aerobic stability, ammoniacal nitrogen (NH3), pH, and microbial population of the silages. The additive increased the crude protein content (P = 0.0062) in corn and sorghum and decreased the LIG content (P = 0.0567). The gas production was not affected (P > 0.05) by the additive and neither between crops. In aerobic stability, we observed that the additive affected the temperature of the sorghum silage (P = 0.0123). The additive decreased NH3 (P = 0.0095) content. The additive increased (P = 0.0441) the lactic acid bacteria population in the pearl millet, corn, and sorghum silages. Thus, the bacterial additive did not improve the fermentation profile and nutritional value of corn, pearl millet, and sorghum silages in plastic bag silos.

scholarly journals Effect of different double-sided plastic films on chemical and fermentation characteristics of corn silage

2021 ◽  
Vol 22 ◽  
Author(s):  
Fernando Braga Cristo ◽  
Mikael Neumann ◽  
Fernando de Souza Sidor ◽  
Maria Eduarda Cozechen Seller ◽  
Daniel Corrêa Plodoviski ◽  
...  
Keyword(s):  
Experimental Design ◽  
Dry Matter ◽  
Corn Silage ◽  
Plastic Films ◽  
Dry Matter Digestibility ◽  
Aerobic Stability ◽  
Randomized Experimental Design ◽  
Polyamide Film

Abstract The objective of this study was to evaluate the efficiency of different double-sided plastic films on chemical and fermentation characteristics, dry matter digestibility, aerobic stability, physical and dry matter losses in corn silages stored in bunker silos. This was a completely randomized experimental design consisting of three treatments: DF110µm - double-sided polyethylene with 110 µm thickness; DF200µm - double-sided polyethylene with 200 µm thickness; and DFBO - oxygen-impermeable film consisted of double-sided polyethylene with 80 µm thickness overlaid with a translucent vacuum polyamide film with 20 µm thickness. The use of DF200µm film increased the ruminal dry matter digestibility by 4.58% and reduced the silage temperature by 3.1 °C, as well as the physical losses of the corn silage were reduced by 118.9 g kg-1 DM using DFBO and 95 g kg-1 DM with DF200µm; DFBO resulted in the highest aerobic stability (127 hours) of corn silage. The use of DF200µm and DFBO is recommended for preserving corn silage in bunker silos.

scholarly journals Influence of Calcium Silicate on Soil Fertility and Corn Morphology

2020 ◽  
Vol 8 (1) ◽  
pp. 51
Author(s):  
Patrick Bezerra Fernandes ◽  
Leandro Pires Bitencourt ◽  
Gustavo De Faria Theodoro ◽  
Uriel De Almeida Curcio ◽  
Willian De Assis Theodoro ◽  
...  
Keyword(s):  
Zea Mays ◽  
Experimental Design ◽  
Soil Fertility ◽  
Calcium Silicate ◽  
Aerial Part ◽  
Soil Acidity ◽  
Control Treatment ◽  
Stem Height ◽  
Alternative Source ◽  
Lower Height

The objective of this study was to evaluate whether the development of corn (Zea mays) and soil fertility are influenced by the application of increasing doses of calcium silicate (CaSiO3) in substitution to limestone. The experiment was carried out under greenhouse conditions and the experimental design was in randomized blocks, in a 3x2+1 factorial arrangement and four replications. The factors were: doses of CaSiO3 (7.62 mg.dm-3, 19.05 mg.dm-3 and 38.09 mg.dm-3) and liming (absence and presence). The soil of the control treatment did not receive limestone or CaSiO3. At 21 days after emergence (DAE), it was found that the control treatment plants had significantly lower height and stem diameter. At 35 DAE was observed that using exclusively CaSiO3 promoted 6% higher stem height values. At 63 DAE, no oscillations were observed in the biomass of aerial part and root between liming and CaSiO3 doses (P>0.05). Using the limestone allied the silicate doses increased in higher concentration of Mg in the soil. The lowest dose of CaSiO3 reduced the concentrations of Ca and Mg. Calcium silicate can be used as an alternative source to limestone for soil acidity correction.

scholarly journals Effect of inoculants combining Lactobacillus buchneri strain LN40177 in different strata of the silo

2019 ◽  
Vol 41 (1) ◽  
pp. 44847
Author(s):  
Tânia Mara Becher Ribas ◽  
Mikael Neumann ◽  
André Martins de Souza ◽  
André Dochwat ◽  
Eduardo Rodrigues de Almeida ◽  
...  
Keyword(s):  
Chemical Composition ◽  
Experimental Design ◽  
Enterococcus Faecium ◽  
Crude Protein ◽  
Dry Matter ◽  
Block Design ◽  
Corn Silage ◽  
Control Treatment ◽  
Disappearance Rate ◽  
Randomized Complete Block Design

The objective of this work was to evaluate the chemical composition and rumen disappearance rate of dry matter of corn silages with inoculants combining L. buchneri strain LN40177 in different strata of the silo. The experimental design was a 3x2 randomized complete block design, with three treatments: Control: corn silage without inoculant; 11CFT: corn silage with inoculant which combines L. buchneri strain LN40177 (1.1 x 1011 CFU g-1) with L. casei (1.1 x 1011 CFU g-1); and 11C33: corn silage with inoculant which combines L. buchneri strain LN40177 (1.1 x 1011 CFU g-1) with L. plantarum (1.1 x 1011 CFU g-1) and Enterococcus faecium (1 x 1010 CFU g-1), associated with two strata of the silo (lower and upper). The silage inoculated with 11C33 presented higher contents of crude protein and NDF and lower hemicellulose content in relation to the control treatment and 11CFT. The use of both inoculants resulted in silages with higher concentrations of soluble nutrients. Lower stratum silage had a higher rumen disappearance rate of dry matter compared to the upper stratum. In general, the combinations of L. buchneri promoted nutritional improvements in corn silage, but in presence of L. casei, there were more outstanding improvements.

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