The effects of Propionibacterium acidipropionici and Lactobacillus plantarum, applied at ensiling, on the fermentation and aerobic stability of low dry matter corn and sorghum silages

2006 ◽  
Vol 33 (5) ◽  
pp. 353-358 ◽  
Author(s):  
I Filya ◽  
E Sucu ◽  
A Karabulut
Keyword(s):  
Lactobacillus Plantarum ◽  
Dry Matter ◽  
Propionibacterium Acidipropionici ◽  
Aerobic Stability

scholarly journals Application of Selected Inoculant Producing Antifungal and Fibrinolytic Substances on Rye Silage with Different Wilting Time

Processes ◽  
2021 ◽  
Vol 9 (5) ◽  
pp. 879
Author(s):  
Seong-Shin Lee ◽  
Jeong-Seok Choi ◽  
Dimas Hand Vidya Paradhipta ◽  
Young-Ho Joo ◽  
Hyuk-Jun Lee ◽  
...  
Keyword(s):  
Lactobacillus Plantarum ◽  
Dry Matter ◽  
Lactobacillus Brevis ◽  
Neutral Detergent Fiber ◽  
Dry Matter Digestibility ◽  
Fiber Digestibility ◽  
Aerobic Stability ◽  
Commercial Inoculant

This research was conducted to determine the effects of selected inoculant on the silage with different wilting times. The ryes were unwilted or wilted for 12 h. Each rye forage was ensiled for 100 d in quadruplicate with commercial inoculant (Lactobacillus plantarum sp.; LPT) or selected inoculant (Lactobacillus brevis 100D8 and Leuconostoc holzapfelii 5H4 at 1:1 ratio; MIX). In vitro dry matter digestibility and in vitro neutral detergent fiber digestibility were highest in the unwilted MIX silages (p < 0.05), and the concentration of ruminal acetate was increased in MIX silages (p < 0.001; 61.4% vs. 60.3%) by the increase of neutral detergent fiber digestibility. The concentration of ruminal ammonia-N was increased in wilted silages (p < 0.001; 34.8% vs. 21.1%). The yeast count was lower in the MIX silages than in the LPT silages (p < 0.05) due to a higher concentration of acetate in MIX silages (p < 0.05). Aerobic stability was highest in the wilted MIX silages (p < 0.05). In conclusion, the MIX inoculation increased aerobic stability and improved fiber digestibility. As a result of the wilting process, ammonia-N in silage decreased but ruminal ammonia-N increased. Notably, the wilted silage with applied mixed inoculant had the highest aerobic stability.

Chemical composition and production of ethanol and other volatile organic compounds in sugarcane silage treated with chemical and microbial additives

2019 ◽  
Vol 59 (4) ◽  
pp. 721
Author(s):  
Lucas Ladeira Cardoso ◽  
Karina Guimarães Ribeiro ◽  
Marcos Inácio Marcondes ◽  
Odilon Gomes Pereira ◽  
Kirsten Weiß
Keyword(s):  
Chemical Composition ◽  
Volatile Organic Compounds ◽  
Lactobacillus Plantarum ◽  
Organic Compounds ◽  
Dry Matter ◽  
Production Systems ◽  
Propionibacterium Acidipropionici ◽  
Pediococcus Pentosaceus ◽  
Ester Formation ◽  
Volatile Organic

Sugarcane silage can be used in animal production systems; however, it is important to apply additives to improve its chemical composition and fermentative quality. We evaluated the effect of chemical (urea and calcium oxide (CaO)) and microbial (Lactobacillus buchneri (LB), Lactobacillus plantarum, Pediococcus pentosaceus, and Propionibacterium acidipropionici) additives on chemical composition, fermentation profile, microorganism population, and production of ethanol and other volatile organic compounds in sugarcane silage. Treatments studied were silage without inoculant (SS), SS with LB, SS with Lactobacillus plantarum and Pediococcus pentosaceus, SS with Lactobacillus plantarum and Propionibacterium acidipropionici, SS with 5 g CaO/kg fresh material (FM) (5CaO), SS with 10 g CaO/kg FM (10CaO), SS with 5 g urea/kg FM (5urea), and SS with 10 g urea/kg FM (10urea). The highest crude protein content (P = 0.001) and the lowest N-linked to fibre content (P = 0.001) occurred when applying urea. None of the treatments reduced the presence of yeast (P = 0.054), but a trend was detected of treatments based on CaO as promising in this Control. The silages treated with CaO had lower ethyl ester and ethanol (average for CaO-based treatments of 0.012 g/kg dry matter and 0.695 g/kg dry matter, respectively), and silages treated with 10urea had less acetone (P = 0.001) and methanol (P = 0.001). The sugarcane silages treated with chemical additive CaO reduced ethanol production and ester formation. There was a high correlation (r = 0.984) between ethyl acetate + ethyl lactate and ethanol contents.

scholarly journals Fermentation Profile, Nutritional Value, and Microbial Population of C4 Grasses Silages with or Without Bacterial Additive

2021 ◽  
Author(s):  
Sarah E. E. Bernardo ◽  
Paulo H. B. Chrisostomo ◽  
Michele G. Camilo ◽  
Danielle F. Baffa ◽  
Elizabeth F. Processi ◽  
...  
Keyword(s):  
Lactobacillus Plantarum ◽  
Pearl Millet ◽  
Nutritional Value ◽  
Microbial Population ◽  
Block Design ◽  
Gas Production ◽  
Propionibacterium Acidipropionici ◽  
Aerobic Stability ◽  
Fermentation Profile

Abstract The objective of the present study was to evaluate the use of bacterial additive (Lactobacillus plantarum and Propionibacterium acidipropionici) on chemical composition, in vitro gas production, pH, losses, aerobic stability, and microbial population of corn, pearl millet, and sorghum silages in plastic bags silos (without vacuum). The experiment was carried out in a randomized block design, in a 2 × 3 factorial scheme, with or without additive ([Control] without additive and Lactobacillus plantarum [2.5 × 1010 cfu/g] and Propionibacterium acidipropionici [2.5 × 1010 cfu/g] Biomax corn, Lallemand, Saint-Simon, France [LP]) and three crops of agricultural interest; pearl millet, sorghum, and corn, with four replicates per treatment. We performed chemical analyses and in vitro gas production to determine the nutritional value of the silages. We also evaluated the aerobic stability, ammoniacal nitrogen (NH3), pH, and microbial population of the silages. The additive increased the crude protein content (P = 0.0062) in corn and sorghum and decreased the LIG content (P = 0.0567). The gas production was not affected (P > 0.05) by the additive and neither between crops. In aerobic stability, we observed that the additive affected the temperature of the sorghum silage (P = 0.0123). The additive decreased NH3 (P = 0.0095) content. The additive increased (P = 0.0441) the lactic acid bacteria population in the pearl millet, corn, and sorghum silages. Thus, the bacterial additive did not improve the fermentation profile and nutritional value of corn, pearl millet, and sorghum silages in plastic bag silos.

scholarly journals Aerobic stability in corn silage (Zea maysL.) ensiled with different microbial additives

2017 ◽  
Vol 39 (4) ◽  
pp. 357 ◽  
Author(s):  
Valter Harry Bumbieris Junior ◽  
Vinícius André De Pietro Guimarães ◽  
Ana Paula De Souza Fortaleza ◽  
Fernando Luiz Massaro Junior ◽  
Gabriella Jorgette de Moraes ◽  
...  
Keyword(s):  
Zea Mays ◽  
Bacillus Subtilis ◽  
Experimental Design ◽  
Lactobacillus Plantarum ◽  
Corn Silage ◽  
Control Treatment ◽  
Propionibacterium Acidipropionici ◽  
Aerobic Stability ◽  
Corn Hybrid ◽  
Plot Design

  This study aimed to assess different microbial additives, regarding the efficiency of aerobic stability in corn silages. The corn hybrid used for silages production was the DKB 310. The treatments consisted of: 1) control treatment without any microbial additive; 2) Treatment with LPPA composed of: Lactobacillus plantarum CCT 0580 3.1 x 1010 CFU g-1 and Propionibacterium acidipropionici CCT 4843 3.1 x 1010 CFU g-1; 3) Treatment with Inoculum, composed of Bacillus subtilis CCT 0089 3.0 x 109 CFU g-1, Lactobacillus plantarum CCT 0580 1.2 x 1010 CFU g-1 and Propionibacterium acidipropionici CCT 4843 1.5 x 1010 CFU g-1 and 4) Treatment LB, composed only of Lactobacillus buchneri CCT 3746 2.6 x 1010 CFU g-1. The experimental design was completely randomized with five replicates for each treatment. For the parameters evaluated daily, we used the split plot design, in which the different silages were assigned to the plots and the time of exposure to air was assigned to the subplots. There was no difference of additives on silage pH in any of the evaluation days. The control silage reached higher temperatures indicating greater vulnerability. All additives had no aerobic instability. Silages made with the use of microbial additives were effective in maintaining the aerobic stability. 

scholarly journals Effect of rehydration with whey and inoculation with Lactobacillus plantarum and Propionibacterium acidipropionici on the chemical composition, microbiological dynamics, and fermentative losses of corn grain silage

2020 ◽  
Vol 41 (6supl2) ◽  
pp. 3351-3364
Author(s):  
William Luiz de Souza ◽  
◽  
Iorrano Andrade Cidrini ◽  
Arnaldo Prata Neiva Júnior ◽  
Mateus Diniz Silva ◽  
...  
Keyword(s):  
Lactobacillus Plantarum ◽  
Dry Matter ◽  
Propionibacterium Acidipropionici ◽  
Ph Values ◽  
Randomized Design ◽  
Fermentation Profile ◽  
Completely Randomized Design ◽  
Material Conservation ◽  
Over Time ◽  
Corn Grain

The objective of this study was to compare rehydrated corn grain silages using water or whey and inoculated (Lactobacillus plantarum and Propionibacterium acidipropionici) or not. We also verified whether rehydration with whey associated with the bacterial inoculant improves material conservation. The treatments were as follows: silages rehydrated with water without inoculant (SWa); silages rehydrated with water and inoculated (SWaI); silages rehydrated with whey without inoculant (SWe); silages rehydrated with whey and inoculated (SWeI). A completely randomized design was used, with three replications, treatments in a 2 × 2 factorial scheme (RE: rehydration with water or whey, and IN: inoculation or addition of water without chlorine), during the following storage periods (T): 0, 4, 8, 16, 32, and 64 days. There was an RE x IN x T interaction (P < 0.001) for dry matter (DM), with lower values over time for SWe and higher values in SWeI. An interaction between RE x IN (P < 0.001) and IN x T (P < 0.001) was observed for pH, with higher values for SWe at 64 days of storage and a reduction from the first days of ensiling for SWaI and SWeI. The microbiological variables showed an RE x IN x T interaction (P < 0.001), with the highest counts of lactic acid bacteria for SWaI and SWeI up to 8 days of storage and subsequent higher counts in SWa and SWe. Higher counts were obtained in SWe. For the variables of fermentative losses, there was an RE x IN x T interaction (P < 0.001), with SWeI showing lower losses. The inoculation associated with whey for rehydration of corn grain improved the fermentation profile of the silage, with lower pH values and reduced losses.

scholarly journals High-moisture corn grain silage inoculated with Propionibacterium acidipropionici and Lactobacillus plantarum in different storage times

2021 ◽  
Vol 10 (1) ◽  
pp. e0410110371
Author(s):  
Rafael Frank ◽  
Marcela Abbado Neres ◽  
Tatiane Fernandes ◽  
Rodrigo André Schöne ◽  
André Sanches de Avila ◽  
...  
Keyword(s):  
Lactobacillus Plantarum ◽  
High Moisture ◽  
Propionibacterium Acidipropionici ◽  
Colony Forming Unit ◽  
Ph Values ◽  
Aerobic Stability ◽  
Storage Times ◽  
And Storage ◽  
Corn Grain ◽  
High Moisture Corn

The aim of this study was to evaluate the chemical composition, growth of microorganisms, and the aerobic stability of high-moisture corn grain silage inoculated with Lactobacillus plantarum + Propionibacterium acidipropionici. The experimental design was completely randomized in a 2 x 5 factorial arrangement with four replications (50 experimental units)  and the treatments were: use or not of microbial inoculant (2 x 105 colony forming unit (CFU) g-1 Lactobacillus plantarum + Propionibacterium acidipropionici) and the storage length for 0, 1, 3, 7, and 14 d. Aerobic stability of silage was evaluated at 28 and 56 d of storage length. The lactic acid bacteria population was influenced by storage length and the greatest values were estimated at 8 d of storage length. Regardless of inoculant application, no enterobacteria were present from 3 d post-ensiling. With respect to mold growth, an interaction between inoculant and storage length was observed wherein molds were most abundant after 3 d of storage in silage that received inoculant. However, at 14 d of storage the use of bacterial inoculant reduced the occurrence of molds. pH values obtained after 3 d of ensiling were less than 4.0 for all the treatments. The high-moisture corn grain silage possessed good fermentative quality. Further, adequate pH values were achieved from the third day of ensilage and were not influenced by the presence of bacterial inoculant.

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