<b>Utilization of low-cost substrates for the production of nystose by <i>Bacillus subtilis</i> natto cct 7712

Current analysis describes the capacity of Bacillus subtilis natto CCT 7712 to produce high amounts of nystose by low-cost substrates available in Brazil, such as commercial sucrose, sugarcane molasses and sugarcane juice. Optimization resulted in a maximum production of 179.77 g L-1 of nystose, averaging 7.49 g L-1 hour-1 of productivity and a 71.73% yield in a medium with 400 g L-1of commercial sucrose and 0.8 g L-1 of MnSO4. Fermentations with sugarcane molasses and sugarcane juice also resulted in a satisfactory production reaching 97.93 and 42.58 g L-1 nystose, respectively. High nystose production in a medium with sugarcane derivatives suggests submerged fermentation with Bacillus subtillis natto CT 7712 as a promising strategy to produce nystose at industrial level.

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Tofu processing wastewater as a low-cost substrate for high activity nattokinase production using Bacillus subtilis

BMC Biotechnology ◽

10.1186/s12896-021-00719-1 ◽

2021 ◽

Vol 21 (1) ◽

Author(s):

Tao Li ◽

Chenyi Zhan ◽

Gege Guo ◽

Zhaoxing Liu ◽

Ning Hao ◽

...

Keyword(s):

Bacillus Subtilis ◽

Nitrogen Source ◽

Low Cost ◽

Operating Cost ◽

Scale Up ◽

Fermentation Medium ◽

Medium Components ◽

Culture Parameters ◽

Critical Components ◽

Industrial Level

Abstract Background Even though tofu is a traditional Chinese food loved by Asian people the wastewater generated during the production of tofu can pollute the environment, and the treatment of this generated wastewater can increase the operating cost of the plant. In this study, the production of nattokinase could be achieved by using the nitrogen source in tofu processing wastewater (TPW) instead of using the traditional nattokinase medium. This meets the need for the low-cost fermentation of nattokinase and at the same time addresses the environmental pollution concerns caused by the wastewater. Bacillus subtilis 13,932 is, a high yielding strain of nattokinase, which is stored in our laboratory. To increase the activity of nattokinase in the tofu process wastewater fermentation medium, the medium components and culture parameters were optimized. Nattokinase with high enzymatic activity was obtained in 7 L and 100 L bioreactors when TPW was used as the sole nitrogen source catalyzed by Bacillus subtilis. Such a result demonstrates that the production of nattokinase from TPW fermentation using B. subtilis can be implemented at an industrial level. Results The peptide component in TPW is a crucial factor in the production of nattokinase. Box–Behnken design (BBD) experiments were designed to optimize various critical components, i.e., Glucose, TPW, MgSO4·7H2O, CaCl2, in nattokinase fermentation media. A maximum nattokinase activity was recorded at 37 °C, pH 7.0, 70 mL liquid medium, and 200 rpm. The highest nattokinase activities obtained from 7 to 100 L bioreactors were 8628.35 ± 113.87 IU/mL and 10,661.97 ± 72.47 IU/mL, respectively. Conclusions By replacing the nitrogen source in the original medium with TPW, there was an increase in the enzyme activity by 19.25% after optimizing the medium and culture parameters. According to the scale-up experiment from conical flasks to 100 L bioreactors, there was an increase in the activity of nattokinase by 47.89%.

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Use of starch manufacturer residue in the production of inoculating biofertilizer

Revista Eletrônica em Gestão Educação e Tecnologia Ambiental ◽

10.5902/2236117038537 ◽

2019 ◽

Vol 23 ◽

pp. 13

Author(s):

Paulo Agenor Alves Bueno ◽

Edgar Lopes Balestri ◽

Rafael De Almeida Vidal Feres Rosiello ◽

Cristian Coelho Silva ◽

Bruna Lopes Gualdi ◽

...

Keyword(s):

Bacillus Subtilis ◽

Culture Medium ◽

Low Cost ◽

Growth Promoters ◽

Trichoderma Spp ◽

Environmental Liabilities ◽

Maximum Production ◽

Plant Growth Promoters ◽

Bacterium Bacillus Subtilis ◽

Bacterium Bacillus

In the industrialization of cassava, effluents are generated capable of causing great impacts to the environment. One of these residues is called manipueira and its generation occurs by pressing the grated cassava mass. An alternative to the use of this industrial residue is its use as a culture medium in the multiplication of microorganisms useful in agriculture in the form of biological inoculants. The objective of this work was to evaluate the effectiveness of different concentrations of manipueira used as substrate in the development of the bacterium Bacillus subtilis and the fungus Trichoderma spp as biological control agents and plant growth promoters, aiming at the low cost of the agricultural protection process. The tests were carried out by means of a triple plating technique in culture medium containing manipueira, molasses and distilled water in different concentrations for both biological agents. The efficiency of the method, substract and organisms was measured through the calculation of colony forming units (CFU). The maximum production of CFU of the fungus Trichoderma spp was 1.2x109 CFU.mL -1 in a concentration of 175mL.L -1 of manipueira, while for the bacterium Bacillus subtilis, the maximum production reached 6.49x1011 CFU .mL -1 in culture with concentration of 125mL -1 of the residue. Compared to commercial inoculants with concentration around 1x108 CFU, the tested cultures were compatible with this value and still managed to overcome it, confirming its efficiency. Therefore, this is an excellent possibility to direct the use of environmental liabilities and to encourage the use of the biofertilizer by farmers who can produce it in their properties in an alternative way and with a low cost of production in relation to the commercial value.

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Co3O4-CuCoO2 hybrid nanoplates as a low-cost and highly active catalyst for producing hydrogen from ammonia borane

New Journal of Chemistry ◽

10.1039/d0nj05524g ◽

2021 ◽

Author(s):

Yufa Feng ◽

Jinyun Liao ◽

Xiaodong Chen ◽

Qingyu Liao ◽

Huize Wang ◽

...

Keyword(s):

Fuel Cell ◽

Active Catalyst ◽

Low Cost ◽

Ammonia Borane ◽

Catalytic Materials ◽

Highly Active ◽

Promising Strategy

Developing low-cost and highly active hydrolysis catalytic materials for the dehydrogenation of hydrogen-rich chemicals is a promising strategy to store and easily release hydrogen for fuel cell applications. In this...

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Bacillus subtilis natto: a non-toxic source of poly-γ-glutamic acid that could be used as a cryoprotectant for probiotic bacteria

AMB Express ◽

10.1186/2191-0855-3-36 ◽

2013 ◽

Vol 3 (1) ◽

pp. 36 ◽

Cited By ~ 39

Author(s):

Aditya R Bhat ◽

Victor U Irorere ◽

Terry Bartlett ◽

David Hill ◽

Gopal Kedia ◽

...

Keyword(s):

Bacillus Subtilis ◽

Glutamic Acid ◽

Probiotic Bacteria ◽

Bacillus Subtilis Natto

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Optimization of Bacillus subtilis natto growth parameters in glycerol-based medium for vitamin K (Menaquinone-7) production in biofilm reactors

Bioprocess and Biosystems Engineering ◽

10.1007/s00449-017-1857-0 ◽

2017 ◽

Vol 41 (2) ◽

pp. 195-204 ◽

Cited By ~ 19

Author(s):

Ehsan Mahdinia ◽

Ali Demirci ◽

Aydin Berenjian

Keyword(s):

Bacillus Subtilis ◽

Vitamin K ◽

Growth Parameters ◽

Biofilm Reactors ◽

Bacillus Subtilis Natto

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Live Bacillus subtilis natto Promotes Rumen Fermentation by Modulating Rumen Microbiota In Vitro

Animals ◽

10.3390/ani11061519 ◽

2021 ◽

Vol 11 (6) ◽

pp. 1519

Author(s):

Meinan Chang ◽

Fengtao Ma ◽

Jingya Wei ◽

Junhao Liu ◽

Xuemei Nan ◽

...

Keyword(s):

Bacillus Subtilis ◽

16S Rrna ◽

Rumen Fluid ◽

Gene Sequencing ◽

16S Rrna Gene Sequencing ◽

Rumen Fermentation ◽

Rrna Gene ◽

Bacillus Subtilis Natto ◽

Rrna Gene Sequencing

Previous studies have shown that Bacillus subtilis natto affects rumen fermentation and rumen microbial community structure, which are limited to detect a few microbial abundances using traditional methods. However, the regulation of B. subtilis natto on rumen microorganisms and the mechanisms of microbiota that affect rumen fermentation is still unclear. This study explored the effects of live and autoclaved B. subtilis natto on ruminal microbial composition and diversity in vitro using 16S rRNA gene sequencing and the underlying mechanisms. Rumen fluid was collected, allocated to thirty-six bottles, and divided into three treatments: CTR, blank control group without B. subtilis natto; LBS, CTR with 109 cfu of live B. subtilis natto; and ABS, CTR with 109 cfu of autoclaved B. subtilis natto. The rumen fluid was collected after 0, 6, 12, and 24 h of fermentation, and pH, ammonia nitrogen (NH3-N), microbial protein (MCP), and volatile fatty acids (VFAs) were determined. The diversity and composition of rumen microbiota were assessed by 16S rRNA gene sequencing. The results revealed LBS affected the concentrations of NH3-N, MCP, and VFAs (p < 0.05), especially after 12 h, which might be attributed to changes in 18 genera. Whereas ABS only enhanced pH and NH3-N concentration compared with the CTR group (p < 0.05), which might be associated with changes in six genera. Supplementation with live B. subtilis natto improved ruminal NH3-N and propionate concentrations, indicating that live bacteria were better than autoclaved ones. This study advances our understanding of B. subtilis natto in promoting ruminal fermentation, providing a new perspective for the precise utilization of B. subtilis natto in dairy rations.

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