Bioinformatic Analysis of rbcL Gene in Lilium
Used sequence diversity of chloroplast rbcL gene sequence to analyzeLiliumphylogeny, structured model of the rbcL protein secondary structure and tertiary structure formLilium superbum. There were 26 variable sites and 12 parsimony-informative sites by analyzing 1378bp rbcL sequences of 17Liliumspecies. The main nucleotide variable type was base transversion. The main variable region form 560bp to 852bp and form1200bp to1371bp. Structured phylogeny tree with N-J method, four types of the 17Liliumspecies were clustered, respectively Asian hybrid group, American hybrid group (two branches), and longiflorum hybrids group. Longiflorum hybrid system (nineLilium species ) was divided into four sub-categories. Structured protein secondary structure of rbcL protein ofLilium superbum, there were 18 alpha helixes, 17 beta sheets and some turn angles. The hydrophobic analysis was hydrophilic protein. 3D model was structured by homology comparative SWISS-Model online. The scores of most amino acid residues in the 3D conformation of the rbcL protein were positive and within are asonable range.