The Distributive Characteristics of Soil Microorganisms at Dry Lake Wetland on Semiarid Grassland

2011 ◽  
Vol 71-78 ◽  
pp. 2882-2886 ◽  
Author(s):  
Yu Qin Shao ◽  
Zi Long Zhao ◽  
Miao Miao Hou ◽  
Hui Min ◽  
Zhong Ling Liu ◽  
...  

This research investigated the distributive characteristics of soil microorganisms at dry lake wetland on semiarid grassland. Four plots, typical steppe (DT), lake side (HB), lake bed (HD), and lake center (HX), were selected from Huitengxile located in semiarid grassland to conducted this investigation. The results showed that the number of aerobic bacteria at DT is 1.07 times that at HB, 1.14 times that at HX, and 1.45 times that at HD. The number of fungi at DT is 3.81 times that at HD, 10.65 times that at HB, and 25.75 times that at HX. There is significant difference between HX, HB and the other two plots (p<0.05). Also, there is significant difference between HD and DT. The number of Spore-forming bacteria at DT is 1.65 times that at HD, 2.15 times that at HB, and 6.35 times that at HX. There is significant difference between HD, HB and the other two plots, as well. The number of actinomycetes at HB is highest which is 2.12 times that at HD, 2.27 times that at DT, and 3.43 times that at HX. Moreover, there is a distinct feature of transverse distribution for soil microorganisms communities at dry lake wetland on semiarid grassland. Excluding actinomycetes, the number of soil microorganisms at dry lake wetland were both lower than that at typical steppe. There is a significant correlation between the total amount of soil microorganisms and microbial biomass C at dry lake wetland. These results can be used as the theoretical foundation for the restoration of degraded lake wetland, and the exploitation and utilization of wetland microbial resources.

2011 ◽  
Vol 356-360 ◽  
pp. 2678-2681 ◽  
Author(s):  
Ji Zhao ◽  
Yu Qin Shao ◽  
Zi Long Zhao ◽  
Zheng Min Li ◽  
Xiao Tong Wu ◽  
...  

In this study, the composition and distribution of dominant soil filamentous microbes , such as fungi and actinomycetes, at dry lake wetland soil on typical steppe were measured through Spread-Plate-Technique; the divergent characteristics of two microbial floras at lake wetland and typical steppe were analyzed; and several indicators of soil filamentous microbes, for example, diversity, richness, evenness, and dominance, were calculated. For fungi, there is significant difference between lake center (LC), typical steppe (TS) & lake bed (LB), lake side (LS) for diversity and richness; and there is significant difference between LC and TS& LB and LS for dominance. For actinomycetes, there is significant difference between LC & LS, and LB& TS for diversity; and there is significant difference between LC&TS for richness; and there is significant difference between LC&LS for evenness. At LC, the most dominant floras is Mucor, accounting for 100%. At LB, Mucor accounts for 26.25% and Penicillium accounts for 45%. At LS, the dominant floras are Mucor, accounting for 48%, and Penicillium, accounting for 34.48%. At TS, Penicillium has become the dominant flora to account for 92.23%. For actinomycetes, the most dominant floras at LC is Griseofuscus, accounting for 55.71%. At LS, the dominant floras are Griseofuscus accounting for18.75%, and balding group, accounting for 20%. At TS, the dominant flora are Griseofuscus, accounting for16.08%, and Flav us, accounting for 14.69%.The results have demonstrated a better understanding toward the development and succession mechanisms of wetland to provide the basis for protection and rational utilizations of dry lake wetland.


OENO One ◽  
2020 ◽  
Vol 54 (4) ◽  
pp. 1143-1157
Author(s):  
Semih Tangolar ◽  
Serpil Tangolar ◽  
Ayfer Alkan Torun ◽  
Melike Ada ◽  
Selçuk Göçmez

Cost effective and environmentally friendly strategies in plant nutrition are prime considerations for sustainable viticulture in the face of ever-increasing stress factors throughout the world. Therefore, the present study aimed to investigate the effects of applying different types of organic and inorganic material [control (C; no application), basaltic pumice (P), commercial dry compost (DC), pruning residue:farm manure mixture (1:2, v:v) compost (PR+FM), straw:farm manure mixture (1:2, v:v) compost (S+FM), pumice:dry compost (P+DC), pumice+straw:farm manure compost (P+(S+FM)) and pumice+pruning residue:farm manure compost (P+(PR+FM))] to ‘Black Magic’ grapevines on their agronomic and nutrient acquisition during two consecutive years: 2017 and 2018.The highest yield (6172 g/vine and 7874 g/vine) and cluster weight (411.4 g and 463.2 g) were obtained for the application of S+FM in both years. In terms of berry weight, the highest value was obtained for P+(PR+FM) in 2017, although no significant difference was found between treatments in 2018. PR+FM slightly accelerated berry ripening. The differences between macro and micro element contents of the leaves were statistically significant. The leaf mineral values obtained from pumice were generally higher whether it was applied alone or in combination with other materials. N, P, Ca, Fe and Mn concentrations were sufficient for all applications, while K, Mg and Zn values were within the limits of deficiency. Regarding CO2 production, P+(PR+FM) application in 2017 and S+FM application in 2018 gave the highest values. The effect of organic materials used on microbial biomass-C in soil was higher for S+FM in 2017 and for S+FM and P+DC in 2018 than the other treatments. The application of P+(PR+FM) in 2017 and PR+FM in 2018 had the highest efficiency in terms of dehydrogenase enzyme activity. Urease and phosphatase enzyme values showed a higher activity for P+(PR+FM) in 2017 and P+DC in 2018 than for the other treatments.In this study, we found that applications of organic and inorganic material generally provided good improvement in terms of the agronomic and nutritional properties examined. In order to be able to provide recommendations for appropriate material, it would be useful to evaluate the accumulated effects of the treatments in further research.


2012 ◽  
Vol 610-613 ◽  
pp. 3017-3021 ◽  
Author(s):  
Ji Zhao ◽  
Yu Qin Shao ◽  
Min Hui ◽  
Jia Yin Lu ◽  
Jing Yu Li

Study on the distributive characteristics of the soil microorganisms at floodplain on wetland of Mongolian plateau. Using the Plate dilution method to determine the 0~10cm, 10~20cm, 20~30cm, 40~50cm and 50~60cm soil samples, soil aerobic bacteria, bacillus type bacteria, fungus and actinomycetes bacteria number, the results show that with the deepening of the soil depth, the number of aerobic bacteria number reduced, the peak value of the number of aerobic bacteria in the 0~10cm soil layer, the minimum number in the 50~60cm soil, 0~10cm and the other four soil there was a significant difference, there was no significant difference between the other four soil layers. The Bacillus type bacteria have a high ratio of total aerobic bacteria in 0~10cm, the surface of the soil with a high ammonification quality but also the best. The peak of the number of fungus in 0~10cm, followed by 10~20cm and 30~40cm and 20~30cm, 40~50cm, 50~60cm no isolated fungus. There is no actinomycetes separation in the 10-3 cfu.g-1dry soil dilution.


1976 ◽  
Vol 41 (4) ◽  
pp. 523-529 ◽  
Author(s):  
Daniel R. Boone ◽  
Harold M. Friedman

Reading and writing performance was observed in 30 adult aphasic patients to determine whether there was a significant difference when stimuli and manual responses were varied in the written form: cursive versus manuscript. Patients were asked to read aloud 10 words written cursively and 10 words written in manuscript form. They were then asked to write on dictation 10 word responses using cursive writing and 10 words using manuscript writing. Number of words correctly read, number of words correctly written, and number of letters correctly written in the proper sequence were tallied for both cursive and manuscript writing tasks for each patient. Results indicated no significant difference in correct response between cursive and manuscript writing style for these aphasic patients as a group; however, it was noted that individual patients varied widely in their success using one writing form over the other. It appeared that since neither writing form showed better facilitation of performance, the writing style used should be determined according to the individual patient’s own preference and best performance.


1993 ◽  
Vol 69 (01) ◽  
pp. 035-040 ◽  
Author(s):  
A M H P van den Besselaar ◽  
R M Bertina

SummaryFour thromboplastin reagents were tested by 18 laboratories in Europe, North-America, and Australasia, according to a detailed protocol. One thromboplastin was the International Reference Preparation for ox brain thromboplastin combined with adsorbed bovine plasma (coded OBT/79), and the second was a certified reference material for rabbit brain thromboplastin, plain (coded CRM 149R). The other two thromboplastin reagents were another rabbit plain brain thromboplastin (RP) with a lower ISI than CRM 149R and a rabbit brain thromboplastin combined with adsorbed bovine plasma (RC). Calibration of the latter two reagents was performed according to methods recommended by the World Health Organization (W. H. O.).The purpose of this study was to answer the following questions: 1) Is the calibration of the RC reagent more precise against the bovine/combined (OBT/79) than against the rabbit/plain reagent (CRM 149R)? 2) Is the precision of calibration influenced by the magnitude of the International Sensitivity Index (ISI)?The lowest inter-laboratory variation of ISI was observed in the calibration of the rabbit/plain reagent (RP) against the other rabbit/plain reagent (CRM 149R) (CV 1.6%). The highest interlaboratory variation was obtained in the calibration of rabbit/plain (RP) against bovine/combined (OBT/79) (CV 5.1%). In the calibration of the rabbit/combined (RC) reagent, there was no difference in precision between OBT/79 (CV 4.3%) and CRM 149R (CV 4.2%). Furthermore, there was no significant difference in the precision of the ISI of RC obtained with CRM 149R (ISI = 1.343) and the rabbit/plain (RP) reagent with ISI = 1.14. In conclusion, the calibration of RC could be performed with similar precision with either OBT/79 or CRM 149R, or RP.The mean ISI values calculated with OBT/79 and CRM 149R were practically identical, indicating that there is no bias in the ISI of these reference preparations and that these reference preparations have been stable since their original calibration studies in 1979 and 1987, respectively.International Normalized Ratio (INR) equivalents were calculated for a lyophilized control plasma derived from patients treated with oral anticoagulants. There were small but significant differences in the mean INR equivalents between the bovine and rabbit thromboplastins. There were no differences in the interlaboratory variation of the INR equivalents, when the four thromboplastins were compared.


1966 ◽  
Vol 53 (4) ◽  
pp. 673-680 ◽  
Author(s):  
Torsten Deckert ◽  
Kai R. Jorgensen

ABSTRACT The purpose of this study was to investigate whether a difference could be demonstrated between crystalline insulin extracted from normal human pancreas, and crystalline insulin extracted from bovine and porcine pancreas. Using Hales & Randle's (1963) immunoassay no immunological differences could be demonstrated between human and pig insulin. On the other hand, a significant difference was found, between pig and ox insulin. An attempt was also made to determine whether an immunological difference could be demonstrated between crystalline pig insulin and crystalline human insulin from non diabetic subjects on the one hand and endogenous, circulating insulin from normal subjects, obese subjects and diabetic subjects on the other. No such difference was found. From these experiments it is concluded that endogenous insulin in normal, obese and diabetic human sera is immunologically identical with human, crystalline insulin from non diabetic subjects and crystalline pig insulin.


2020 ◽  
Vol 17 (2) ◽  
pp. 172
Author(s):  
HARMAN AGUSAPUTRA ◽  
MARIA SUGENG ◽  
AYLY SOEKAMTO ◽  
ATIK WULANDARI

<p><strong>Abstract</strong></p><p><strong>Background:</strong> Hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>) as antiseptic has been used frequently to clean woundsin in hospitals and clinics. Hydrogen peroxide has the effectof strong oxidative that can kill pathogens. It can clean up debris and necrotic tissuesin wounds. Hydrogen peroxidealso has hemostatic effect that can help to stop bleeding. Besides antiseptic effects, hydrogen peroxide i s suspected of having negative effect in wound healing. Hydrogen peroxide presumably could cause delayed wound healing by exudate formation and delayed epithelial growth.</p><p><strong>Method</strong>: This study was conducted in the laboratory using 48 white mice that were divided into 2 groups. All the mice were purposely wounded. Afterwards in one group the wounds were clean up using hydrogen peroxide, while in the other group without hydrogen peroxide as control. The wounds of both groups were observed on day 1, day 3 and day 7. On day 1 and day 3, both groups did not show significant difference.</p><p><strong>R</strong><strong>esult</strong> : on day 7 showed that the wound healing in hydrogen peroxide group were delayed. Fifty percent of them had the formation of exudate and 62.5% of them showed delayed epithelial growth.</p><p><strong>Conclusion </strong>: This study could show hydrogen peroxide as wound antiseptic has delayed wound healing effect.</p><p><strong>Keyword</strong>: hydrogen peroxide, wound healing</p>


2017 ◽  
Vol 05 (02) ◽  
pp. 084-089
Author(s):  
Alisha Dhingra ◽  
Ashu Gupta ◽  
Anshu Minocha ◽  
Nayantara Sen

Abstract Aim: The aim of this study was to compare the reversal of shear bond strength of composite to bleached enamel immediately after bleaching followed by application of various antioxidant solutions. Material and Methods: Seventy central incisors were divided into seven groups. Groups I and II served as unbleached and bleached controls respectively. Groups III, IV, V, VI and VII served as the experimental groups and were subjected to 37.5% hydrogen peroxide bleaching followed by 10 min application of 10% sodium ascorbate, 25% alpha-tocopherol, 6.5% grape seed extract, 5% lycopene and 5% green tea extract respectively. Following composite bonding, shear bond strength was determined and the results were analyzed using ANOVA followed by Post Hoc Multiple Comparisons test. Results: The bond strength values for Group I (positive control) were maximum and significantly different than all the other groups except Grape seed extract group (Group V). When compared to Group II (bleached control), all the groups showed significantly higher bond strength. Significant difference in the bond strength values were seen between Group III (10% sodium ascorbate) and Group V. Also values for Group V were significantly different from Group VI (5% lycopene). All the other values showed insignificantly different results. Conclusion: All the antioxidant solutions improved the shear bond strength values after bleaching but only Grape seed extract application reversed the values to the non bleached levels. Lycopene was least effective. Other antioxidants showed comparable results.


Animals ◽  
2021 ◽  
Vol 11 (2) ◽  
pp. 309
Author(s):  
Deise Aline Knob ◽  
André Thaler Neto ◽  
Helen Schweizer ◽  
Anna C. Weigand ◽  
Roberto Kappes ◽  
...  

Crossbreeding in dairy cattle has been used to improve functional traits, milk composition, and efficiency of Holstein herds. The objective of the study was to compare indicators of the metabolic energy balance, nonesterified fatty acids (NEFA), beta-hydroxybutyrate (BHBA), glucose, body condition score (BCS) back fat thickness (BFT), as well as milk yield and milk composition of Holstein and Simmental cows, and their crosses from the prepartum period until the 100th day of lactation at the Livestock Center of the Ludwig Maximilians University (Munich, Germany). In total, 164 cows formed five genetic groups according to their theoretic proportion of Holstein and Simmental genes as follows: Holstein (100% Holstein; n = 9), R1-Hol (51–99% Holstein; n = 30), first generation (F1) crossbreds (50% Holstein, 50% Simmental; n = 17), R1-Sim (1–49% Holstein; n = 81) and Simmental (100% Simmental; n = 27). The study took place between April 2018 and August 2019. BCS, BFT blood parameters, such as BHBA, glucose, and NEFA were recorded weekly. A mixed model analysis with fixed effects breed, week (relative to calving), the interaction of breed and week, parity, calving year, calving season, milking season, and the repeated measure effect of cow was used. BCS increased with the Simmental proportion. All genetic groups lost BCS and BFT after calving. Simmental cows showed lower NEFA values. BHBA and glucose did not differ among genetic groups, but they differed depending on the week relative to calving. Simmental and R1-Sim cows showed a smaller effect than the other genetic groups regarding changes in body weight, BCS, or back fat thickness after a period of a negative energy balance after calving. There was no significant difference for milk yield among genetic groups, although Simmental cows showed a lower milk yield after the third week after calving. Generally, Simmental and R1-Simmental cows seemed to deal better with a negative energy balance after calving than purebred Holstein and the other crossbred lines. Based on a positive heterosis effect of 10.06% for energy corrected milk (ECM), the F1, however, was the most efficient crossbred line.


2011 ◽  
Vol 142 ◽  
pp. 233-237
Author(s):  
Han Wen Yuan ◽  
Fang Chen ◽  
Qiao Qing Xu ◽  
Shi Yuan Gong ◽  
Zhang Jie Chu ◽  
...  

The effects of stocking density on various growth parameters and sex reversal in the rice field eel (Monopterus albus) were investigated by evaluating steroid hormone, the gonadosomatic index (GSI) and sex ratio. Fish (mean initial weight of 9.88 ± 0.70 g) were stocked to densities of 4, 20, 36, 52, 68, 84 or 100 fish/m2 in cages (2 m × 1 m × 1.5 m) in a pond, with five replicate cages for each density. Fish were fed for 420 days on a formulated isocaloric diet containing 40.22% crude protein and 12.86 MJ/kg. Serum E2 and T concentrations were determined by radioimmunoassays. There were no significant differences in final bodyweight (FBW) and daily weight gain (DWG) for fish in the groups containing less than 52 fish/m2, while FBW and DWG were significant lower in groups at 68 fish/m2 or more. Specific growth rate (SGR) differed significantly between the 84 and 100 fish/m2 groups compared with the 4, 20 and 36 fish/m2 groups. There was no significant difference in SGR in groups containing less than 84 fish/m2. There were no significant difference between the 4 and 20 fish/m2 groups in net yield (NY), but these groups did differ significantly from the other groups. The final condition factors of fish stocked at 84 or 100 fish/m2 were significantly lower than fish stocked at the other densities. E2 and T hormone concentrations declined as stocking density increased, while GSI values increased initially but then reduced with increasing stocking density. Female ratio and survival decreased with increasing stocking density. The proportion of male fish was significantly greater in the three highest stocking density groups. Therefore, high stocking density may promote sex change from female to male in M. albus.


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