Applied Orthogonal Experiment Design for Optimization of Ultrasonic-Assisted Extraction of Polysaccharides from Agaricus bisporus Mycelia

2014 ◽  
Vol 1010-1012 ◽  
pp. 1779-1782 ◽  
Author(s):  
Quan Sheng Chen ◽  
Tao Ying Zhou ◽  
Xing Yuan Li ◽  
Yu Bi ◽  
Hui De Yang
Keyword(s):  
Agaricus Bisporus ◽  
Orthogonal Experiment ◽  
Experiment Design ◽  
Ultrasonic Power ◽  
Extraction Temperature ◽  
Orthogonal Experiment Design ◽  
Solid Ratio ◽  
Assisted Extraction ◽  
Ultrasonic Time ◽  
Extraction Parameters

The purpose of this study to establish the optimum ultrasound-assisted extraction (UAE) parameters of Polysaccharides from Agaricus bisporus mycelia (PABM). Single factor experiment was employed to determine the preliminary range of the extraction parameters including liquid-solid ratio, ultrasonic time, extraction temperature, and ultrasonic power. Then, an orthogonal experiment design was employed to determine the best extraction parameters of PABM. the results showed that the optimal UAE parameters of PABM was liquid-solid ratio of 40 ml/g, ultrasonic time of 70 min, extraction temperature of 55°C, and ultrasonic power of 660 W.

Ultrasound-Assisted Extraction of Polysaccharides from Pleurotus eryngii (DC. ex Fr.) Quel

2012 ◽  
Vol 610-613 ◽  
pp. 3410-3415
Author(s):  
Yu Qin Tang ◽  
Yi Tao Zhao
Keyword(s):  
Orthogonal Experiment ◽  
Extraction Yield ◽  
Pleurotus Eryngii ◽  
Extraction Time ◽  
Ultrasonic Power ◽  
Extraction Temperature ◽  
Ultrasound Assisted Extraction ◽  
Power Extraction ◽  
Assisted Extraction ◽  
Ultrasound Assisted

The purpose of this study was to select the optimum ultrasound-assisted extraction (UAE) conditions for the extraction of polysaccharides from Pleurotus eryngii (DC. ex Fr.) Que (P. eryngii ). The main factors that affect the extraction yield of polysaccharides such as solid:liquid ratio, ultrasonic power, extraction time and extraction temperature were studied individually. An orthogonal experiment was designed to optimize the extraction parameters. It was found that UAE method was a reliable, simple and effective method for fast extraction of polysaccharides from P. eryngii. The optimum UAE conditions were as followings: Solid:liquid ratio of 1:35, Ultrasonic power of 55 W, Extraction time of 30 min and extraction temperature of 45°C.

Optimization for the Process of Chlorophyll a Extraction by Orthogonal Method

2014 ◽  
Vol 1051 ◽  
pp. 423-427
Author(s):  
Jun Sen Wu ◽  
Chen Chen Liu ◽  
Bing Li ◽  
Xiu Zhi Wan
Keyword(s):  
Chlorophyll A ◽  
Orthogonal Experiment ◽  
Ultrasonic Power ◽  
Assisted Extraction ◽  
Ultrasonic Time ◽  
Ultrasonic Assisted ◽  
Ultrasonic Assisted Extraction ◽  
Extraction Agent ◽  
Optimum Extraction ◽  
Single Factor Experiment

The 90% acetone as extraction agent used in order to obtain the ultrasonic assisted extraction conditions of chlorophyll a. The ultrasonic power, ultrasonic time, centrifugation time and rotational speed as the four study factors and the chlorophyll a concentration as the target, here, the L9(34) orthogonal table of orthogonal experiment design are used in order to determine the optimum extraction contractions, basing on the single factor experiment. The experimental results shows the algae containing water can get better extraction effect, after ultrasonic 30 minutes in 225W and 6000r/min speed centrifugal 9 minutes and then mixing, repeat ultrasound centrifugation steps.

Optimizing the Conditions for Polysaccharides Ultrasonic-Assisted Extraction in Mycelium of Paecilomyces Tenuipes Pt196 Using Statistical Approach

2013 ◽  
Vol 756-759 ◽  
pp. 180-185
Author(s):  
Lin Na Du ◽  
Wen Ting Bu ◽  
Wen Lin Pan ◽  
Chen Shao ◽  
Jia Hui Lu ◽  
...  
Keyword(s):  
Neural Network ◽  
Genetic Algorithm ◽  
Artificial Neural Network ◽  
Ultrasonic Power ◽  
Box Behnken Design ◽  
Solid Ratio ◽  
Assisted Extraction ◽  
Ultrasonic Time ◽  
Ultrasonic Assisted ◽  
Artificial Neural

In order to enhance the extraction rate of polysaccharides, a series of statistical approach was used to optimize the ultrasonic-assisted extraction conditions from the mycelium of Paecilomyces tenuipes Pt196. [Metho The optimization of conditions was carried out in two stages. Firstly, the effects of various experimental parameters considered for the investigation (ultrasonic power, ultrasonic time and liquid-solid ratio) were studied using the method of single factor test design experiments. Secondly, a 15-run Box-Behnken design was performed to optimize the extraction conditions of polysaccharides. The experimental results of Box-Behnken design were analyzed by response surface methodology and artificial neural network together with genetic algorithm. [Result The optimum conditions for polysaccharides extraction obtained by the application of artificial neural network-genetic algorithm were ultrasonic time 345s, ultrasonic power 320 W and liquid-solid ratio 95 mL·g-1. [Conclusion Artificial neural network-genetic algorithm can effectively select the best extraction conditions of polysaccharides in this study.

Study on Extraction Process of Technics of Litsea Cubeba Oil

2014 ◽  
Vol 602-605 ◽  
pp. 81-85 ◽  
Author(s):  
Zuo Ren Liu ◽  
Yan Fen Zhang ◽  
Yong Guang Bi ◽  
Hai Ling Li
Keyword(s):  
Visual Analysis ◽  
Extraction Process ◽  
Oil Extraction ◽  
Ultrasonic Power ◽  
Extraction Temperature ◽  
Litsea Cubeba ◽  
Assisted Extraction ◽  
Ultrasonic Time ◽  
Ultrasonic Assisted ◽  
Ultrasonic Assisted Extraction

Litsea cubeba fruit as raw material in this experiment, the use of ultrasonic-assisted method of litsea cubeba oil extraction process are discussed. The effects of several ultrasonic processing parameters, such as ratio of liquid to solid, extraction temperature, ultrasonic power and extraction time on the extraction of litsea cubeba oil were investigated assisted by ultrasonic wave. And the L9 (34) orthogonal test was used to research this four factors, the result from the visual analysis indicated that the optimal extraction process was gotten when ratio of liquid to solid was 14:1 , ultrasonic time was 50min, ultrasonic power is 320W, ultrasonic temperature is 60 °C.Those experimental results showed that ultrasonic-assisted extraction of the Litsea cubeba oil is practicable, and this method is simple and efficient. With the preliminary investigation of the ultrasonic-assisted extraction of Litsea Cubeba oil, the oil yield reached up to 41.35%, this value is the highest of all the oil yields from the current oil extraction methods, and it is worthy of further exploration .

scholarly journals Ultrasound-Assisted Extraction Optimization of α-Glucosidase Inhibitors from Ceratophyllum demersum L. and Identification of Phytochemical Profiling by HPLC-QTOF-MS/MS

Molecules ◽  
2020 ◽  
Vol 25 (19) ◽  
pp. 4507
Author(s):  
Zhen Li ◽  
Zongcai Tu ◽  
Hui Wang ◽  
Lu Zhang
Keyword(s):  
Chinese Herb ◽  
Ceratophyllum Demersum ◽  
Ultrasonic Power ◽  
Extraction Temperature ◽  
Ultrasound Assisted Extraction ◽  
Solid Ratio ◽  
Glucosidase Inhibitors ◽  
Assisted Extraction ◽  
Ic50 Value ◽  
Ultrasound Assisted

Ceratophyllum demersum L. (CDL) is a traditional Chinese herb to treat many diseases, but research on its anti-diabetic activity is not available. In this research, the α-glucosidase inhibitory ability and phytochemical constituents of CDL extract were firstly studied. Optimal ultrasound-assisted extraction conditions for α-glucosidase inhibitors (AGIs) were optimized by single factor experiment and response surface methodology (RSM), which was confirmed as 70% methanol, liquid-to-solid ratio of 43 (mL/g), extraction time of 54 min, ultrasonic power of 350 W, and extraction temperature of 40 °C. The lowest IC50 value for α-glucosidase inhibition was 0.15 mg dried material/mL (mg DM/mL), which was much lower than that of acarbose (IC50 value of 0.64 mg DM/mL). In total, 80 compounds including 8 organic acids, 11 phenolic acids, 25 flavonoids, 21 fatty acids, and 15 others were identified or tentatively identified from CDL extract by HPLC-QTOF-MS/MS analysis. The results suggested that CDL could be a potential source of α-glucosidase inhibitors. It can also provide useful phytochemical information for research into other bioactivities.

scholarly journals Optimization of Ionic Liquid Based Simultaneous Ultrasonic- and Microwave-Assisted Extraction of Rutin and Quercetin from Leaves of Velvetleaf (Abutilon theophrasti) by Response Surface Methodology

2014 ◽  
Vol 2014 ◽  
pp. 1-11 ◽  
Author(s):  
Chunjian Zhao ◽  
Zhicheng Lu ◽  
Chunying Li ◽  
Xin He ◽  
Zhao Li ◽  
...  
Keyword(s):  
Ionic Liquid ◽  
Extraction Time ◽  
Conventional Heating ◽  
Ultrasonic Power ◽  
Extraction Temperature ◽  
Microwave Assisted Extraction ◽  
Solid Ratio ◽  
Microwave Assisted ◽  
Assisted Extraction ◽  
Influential Parameters

An ionic liquids based simultaneous ultrasonic and microwave assisted extraction (ILs-UMAE) method has been proposed for the extraction of rutin (RU), quercetin (QU), from velvetleaf leaves. The influential parameters of the ILs-UMAE were optimized by the single factor and the central composite design (CCD) experiments. A 2.00 M 1-butyl-3-methylimidazolium bromide ([C4mim]Br) was used as the experimental ionic liquid, extraction temperature 60°C, extraction time 12 min, liquid-solid ratio 32 mL/g, microwave power of 534 W, and a fixed ultrasonic power of 50 W. Compared to conventional heating reflux extraction (HRE), the RU and QU extraction yields obtained by ILs-UMAE were, respectively, 5.49 mg/g and 0.27 mg/g, which increased, respectively, 2.01-fold and 2.34-fold with the recoveries that were in the range of 97.62–102.36% for RU and 97.33–102.21% for QU with RSDs lower than 3.2% under the optimized UMAE conditions. In addition, the shorter extraction time was used in ILs-UMAE, compared with HRE. Therefore, ILs-UMAE was a rapid and an efficient method for the extraction of RU and QU from the leaves of velvetleaf.

Optimization of Ultrasound-Assisted Extraction of Polysaccharides from the Chinese Medicinal Plant Rhodiola Sachalinensis A. BOR

2013 ◽  
Vol 448-453 ◽  
pp. 1085-1088
Author(s):  
Chuan Jun Zhang ◽  
Feng Lin Li
Keyword(s):  
Medicinal Plant ◽  
Extraction Time ◽  
Ultrasonic Power ◽  
Extraction Temperature ◽  
Ultrasound Assisted Extraction ◽  
Rhodiola Sachalinensis ◽  
Solid Ratio ◽  
Assisted Extraction ◽  
Ultrasound Assisted ◽  
Chinese Medicinal Plant

Ultrasound-assisted extraction (UAE) of polysaccharides from the Chinese medicinal plantRhodiola sachalinensisA. BOR (PRs) was studied. The effects of four factors (extraction time, ultrasonic power, liquid-solid ratio and extraction temperature) on the extraction yield of PRs were investigated by the single-factor test, and the optimum UAE conditions were investigated by the orthogonal test. The results demonstrated that the optimum UAE conditions for PRs were as followings: extraction time of 30 min, ultrasonic power of 85 W, liquid-solid ratio of 45 ml/g, and extraction temperature of 45°C. The present data might provide a theoretic base for full utilization ofRhodiola sachalinensisA. BOR.

Study on the Preparation of a New Tofu Coagulant by the Acidic Components of Schisandra

2013 ◽  
Vol 310 ◽  
pp. 20-23
Author(s):  
Jian Li ◽  
Lu Wang ◽  
Ning Liu ◽  
Chen Chen Li
Keyword(s):  
Design Method ◽  
Orthogonal Experiment ◽  
Ultrasonic Power ◽  
Extraction Temperature ◽  
Light Yellow ◽  
Assisted Extraction ◽  
Ultrasonic Assisted ◽  
Solid Foundation ◽  
Acidic Components ◽  
Better Than

Using the acidic components of Medicine-food Schisandra researched a new bean curd coagulant. The acid of Schisandra was extracted by ultrasonic-assisted extraction, which was better than conventional extraction. The optimum condition was as follow: extraction temperature 75°C, ultrasonic extraction time 15min, ratio of material to liquid 1:7, ultrasonic power 70%. The optimum technology was selected for manufacture of tofu with natural organic acid coagulant, which determined by orthogonal experiment design method. The concentrations of soybean milk, the addition of coagulant and temperature of adding coagulant were 1:11, 0.3g/ 100mL soymilk and 70°C respectively. Under the optimal conditions, the product was light yellow and had fragrant smell of beans and Schisandra. The study has laid a solid foundation for developing the new natural tofu coagulant.

scholarly journals Application of Ionic Liquid-Based Ultrasonic-Assisted Extraction of Flavonoids from Bamboo Leaves

Molecules ◽  
2018 ◽  
Vol 23 (9) ◽  
pp. 2309 ◽  
Author(s):  
Liling Wang ◽  
Minge Bai ◽  
Yuchuan Qin ◽  
Bentong Liu ◽  
Yanbin Wang ◽  
...  
Keyword(s):  
Ionic Liquid ◽  
Ionic Liquids ◽  
Large Scale ◽  
Ultrasonic Power ◽  
Solid Ratio ◽  
Bamboo Leaves ◽  
Assisted Extraction ◽  
Ultrasonic Time ◽  
Ultrasonic Assisted ◽  
Ultrasonic Assisted Extraction

Ionic liquids (ILs), known as environmentally benign “green” solvents, were developed as an optimal solvent for the green extraction and separation field. In this paper, an ionic liquid-based ultrasonic-assisted extraction (IL-UAE) of flavonoids (FVs) from bamboo leaves of Phyllostachys heterocycla was developed for the first time. First, 1-butyl-3-methylimidazolium bromide ([Bmim] Br), with the best extraction efficiency, was selected from fifteen ionic liquids with diverse structure, like carbon chains or anions. Then, the influencing parameters of ionic liquid (IL) concentration, liquid-solid ratio, ultrasonic time, and ultrasonic power, were investigated by single factor tests, and further optimized using response surface methodology (RSM). In the optimization experiment, the best conditions were 1.5 mol/L [BMIM]Br aqueous solution, liquid-solid ratio 41 mL/g, ultrasonic time 90 min, and ultrasonic power 300 W. Furthermore, the microstructures of bamboo leaves and the recovery of FVs and [BMIM]Br were also studied. Therefore, this simple, green and effective IL-UAE method has potentiality for the extraction of FVs from bamboo leaves for the large-scale operations.

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