Ultrasound-Assisted Extraction Optimization of α-Glucosidase Inhibitors from Ceratophyllum demersum L. and Identification of Phytochemical Profiling by HPLC-QTOF-MS/MS

Ceratophyllum demersum L. (CDL) is a traditional Chinese herb to treat many diseases, but research on its anti-diabetic activity is not available. In this research, the α-glucosidase inhibitory ability and phytochemical constituents of CDL extract were firstly studied. Optimal ultrasound-assisted extraction conditions for α-glucosidase inhibitors (AGIs) were optimized by single factor experiment and response surface methodology (RSM), which was confirmed as 70% methanol, liquid-to-solid ratio of 43 (mL/g), extraction time of 54 min, ultrasonic power of 350 W, and extraction temperature of 40 °C. The lowest IC50 value for α-glucosidase inhibition was 0.15 mg dried material/mL (mg DM/mL), which was much lower than that of acarbose (IC50 value of 0.64 mg DM/mL). In total, 80 compounds including 8 organic acids, 11 phenolic acids, 25 flavonoids, 21 fatty acids, and 15 others were identified or tentatively identified from CDL extract by HPLC-QTOF-MS/MS analysis. The results suggested that CDL could be a potential source of α-glucosidase inhibitors. It can also provide useful phytochemical information for research into other bioactivities.

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Optimization of Ultrasound-Assisted Extraction of Polysaccharides from the Chinese Medicinal Plant Rhodiola Sachalinensis A. BOR

Applied Mechanics and Materials ◽

10.4028/www.scientific.net/amm.448-453.1085 ◽

2013 ◽

Vol 448-453 ◽

pp. 1085-1088

Author(s):

Chuan Jun Zhang ◽

Feng Lin Li

Keyword(s):

Medicinal Plant ◽

Extraction Time ◽

Ultrasonic Power ◽

Extraction Temperature ◽

Ultrasound Assisted Extraction ◽

Rhodiola Sachalinensis ◽

Solid Ratio ◽

Assisted Extraction ◽

Ultrasound Assisted ◽

Chinese Medicinal Plant

Ultrasound-assisted extraction (UAE) of polysaccharides from the Chinese medicinal plantRhodiola sachalinensisA. BOR (PRs) was studied. The effects of four factors (extraction time, ultrasonic power, liquid-solid ratio and extraction temperature) on the extraction yield of PRs were investigated by the single-factor test, and the optimum UAE conditions were investigated by the orthogonal test. The results demonstrated that the optimum UAE conditions for PRs were as followings: extraction time of 30 min, ultrasonic power of 85 W, liquid-solid ratio of 45 ml/g, and extraction temperature of 45°C. The present data might provide a theoretic base for full utilization ofRhodiola sachalinensisA. BOR.

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Optimization of Ultrasound-Assisted Extraction of Antioxidant Polyphenols from the Seed Coats of Red Sword Bean (Canavalia gladiate (Jacq.) DC.)

Antioxidants ◽

10.3390/antiox8070200 ◽

2019 ◽

Vol 8 (7) ◽

pp. 200 ◽

Cited By ~ 5

Author(s):

Yue Zhou ◽

Xiao-Yu Xu ◽

Ren-You Gan ◽

Jie Zheng ◽

Ya Li ◽

...

Keyword(s):

Extraction Method ◽

Natural Antioxidants ◽

Extraction Time ◽

Antioxidant Compounds ◽

Extraction Temperature ◽

Ultrasound Assisted Extraction ◽

Solid Ratio ◽

Assisted Extraction ◽

Ultrasound Assisted ◽

Seed Coats

The seed coat of red sword bean (Canavalia gladiata (Jacq.) DC.) is rich in antioxidant polyphenols. It is often discarded as a byproduct with the consumption of red sword bean, since it is very thick and not consumed by people. The aim of this study was to develop an ultrasound-assisted extraction method to extract natural antioxidants from the seed coats. The extraction process was optimized by using response surface methodology. After the single-factor experiments, three key factors, including ethanol concentration, liquid/solid ratio, and extraction time, were selected and their interactions were studied using a central composite design. The optimal extraction condition was 60.2% hydroethanol, a liquid/solid ratio of 29.3 mL/g, an extraction time of 18.4 min, an extraction temperature of 50 °C, and ultrasound power of 400 W. Under the optimal conditions, antioxidant activity of the extract was 755.98 ± 10.23 μmol Trolox/g dry weight (DW), much higher than that from maceration (558.77 ± 14.42 μmol Trolox/g DW) or Soxhlet extraction (479.81 ± 12.75 μmol Trolox/g DW). In addition, the main antioxidant compounds in the extract were identified and quantified by high-performance liquid chromatography–diode array detection–tandem mass spectrometry (HPLC–DAD–MS/MS). The concentrations of digalloyl hexoside, methyl gallate, gallic acid, trigalloyl hexoside, and digallic acid were 15.30 ± 0.98, 8.85 ± 0.51, 8.76 ± 0.36, 4.27 ± 0.21, and 2.89 ± 0.13 mg/g DW. This study provides an efficient and green extraction method for the extraction of natural antioxidants from the bean coat of red sword bean. The extract of antioxidants might be added into functional foods or nutraceuticals with potential beneficial functions.

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Ultrasound-Assisted Extraction of Polysaccharides from Pleurotus eryngii (DC. ex Fr.) Quel

Advanced Materials Research ◽

10.4028/www.scientific.net/amr.610-613.3410 ◽

2012 ◽

Vol 610-613 ◽

pp. 3410-3415

Author(s):

Yu Qin Tang ◽

Yi Tao Zhao

Keyword(s):

Orthogonal Experiment ◽

Extraction Yield ◽

Pleurotus Eryngii ◽

Extraction Time ◽

Ultrasonic Power ◽

Extraction Temperature ◽

Ultrasound Assisted Extraction ◽

Power Extraction ◽

Assisted Extraction ◽

Ultrasound Assisted

The purpose of this study was to select the optimum ultrasound-assisted extraction (UAE) conditions for the extraction of polysaccharides from Pleurotus eryngii (DC. ex Fr.) Que (P. eryngii ). The main factors that affect the extraction yield of polysaccharides such as solid:liquid ratio, ultrasonic power, extraction time and extraction temperature were studied individually. An orthogonal experiment was designed to optimize the extraction parameters. It was found that UAE method was a reliable, simple and effective method for fast extraction of polysaccharides from P. eryngii. The optimum UAE conditions were as followings: Solid:liquid ratio of 1:35, Ultrasonic power of 55 W, Extraction time of 30 min and extraction temperature of 45°C.

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Optimization of Ultrasound-Assisted Extraction of Oil from Camellia(Camellia oleifera abel) Seed

Advanced Materials Research ◽

10.4028/www.scientific.net/amr.236-238.1854 ◽

2011 ◽

Vol 236-238 ◽

pp. 1854-1858 ◽

Cited By ~ 2

Author(s):

Xue Hui Wu ◽

Li Li

Keyword(s):

Ultrasonic Treatment ◽

Treatment Time ◽

Treatment Temperature ◽

Ultrasonic Power ◽

Ultrasound Assisted Extraction ◽

Camellia Oil ◽

Solid Ratio ◽

Assisted Extraction ◽

Ultrasound Assisted ◽

Main Component

Camellia oil is the main component of the camellia seed and has many beneficial functions to human health. An investigation into ultrasound- assisted extraction was conducted for the oil extraction from camellia seed. The optimized condition of extraction parameters obtained with the response surface methodology (RSM) at a four-variable, three-level experiment Box–Behnken design (BBD), was as follows: liquid-solid ratio, 5mL/g;ultrasonic power,155W;ultrasonic treatment time,31 min; and ultrasonic treatment temperature, 55°C, under these conditions, the oil yeild was 44.55%. GC–MS analysis showed that the content of unsaturated fatty acid in camellia oil was up to 84.47% and the content of oleic acid was over 76.15%.

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Optimization of ultrasound-assisted extraction of bioactive compounds from Gomphrena celosioides Mart. using response surface methodology

Bulletin of the Chemical Society of Ethiopia ◽

10.4314/bcse.v34i2.3 ◽

2020 ◽

Vol 34 (2) ◽

pp. 237-248

Author(s):

N. T. H. Yen ◽

L. P. T. Quoc

Keyword(s):

Bioactive Compounds ◽

Radical Scavenging ◽

Extraction Time ◽

Extraction Temperature ◽

Ultrasound Assisted Extraction ◽

Solid Ratio ◽

Assisted Extraction ◽

Ultrasound Assisted ◽

Gomphrena Celosioides ◽

Experimental Values

In this study, ultrasound-assisted extraction (UAE) was used to extract bioactive compounds from Gomphrena celosioides Mart. Central composite face design (CCF) was used to optimize the influences of extraction factors on total phenolics content (TPC), total flavonoids content (TFC), and 1,1-diphenyl-2-picrylhydrazyl free radical scavenging capacity (DPPH-RSC). The results obtained showed that all factors (solvent to solid ratio, extraction time and extraction temperature) strongly affected TPC, TFC, and DPPH-RSC. The optimal extraction conditions for TPC (3.123 mg GAE/g DW), TFC (1.736 mg QE/g DW), and DPPH-RSC (64.118%) were solvent to solid ratio of 27.3/1 (mL/g), extraction time of 40.2 min, and extraction temperature of 69 oC. In addition, under the optimal conditions, the results pointed out that the experimental values agreed with those predicted. Hence, this model has successfully optimized the extraction conditions for TPC, TFC and DPPH-RSC of Gomphrena celosioides extract. KEY WORDS: Antioxidant activity, CCF, Flavonoids, Model, Phenolic compounds, Regression equation Bull. Chem. Soc. Ethiop. 2020, 34(2), 237-248 DOI: https://dx.doi.org/10.4314/bcse.v34i2.3

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Optimization of Ultrasound Assisted Extraction (UAE) of Kinsenoside Compound from Anoectochilus roxburghii (Wall.) Lindl by Response Surface Methodology (RSM)

Molecules ◽

10.3390/molecules25010193 ◽

2020 ◽

Vol 25 (1) ◽

pp. 193 ◽

Cited By ~ 3

Author(s):

Biyun Yang ◽

Mengyuan Zhang ◽

Haiyong Weng ◽

Yong Xu ◽

Lihui Zeng

Keyword(s):

Response Surface ◽

High Performance ◽

Dry Weight ◽

Methanol Concentration ◽

Ultrasonic Power ◽

Ultrasound Assisted Extraction ◽

Solid Ratio ◽

Assisted Extraction ◽

Anoectochilus Roxburghii ◽

Ultrasound Assisted

The purpose of this study was to establish an extraction method for the kinsenoside compound from the whole plant Anoectochilus roxburghii. Ultrasound assisted extraction (UAE) and Ultra-high performance liquid chromatography (UPLC) method were used to extract and determine the content of kinsenoside, while response surface method (RSM) was used to optimize the extraction process. The best possible range for methanol concentration (0–100%), the liquid-solid ratio (5:1–30:1 mL/g), ultrasonic power (240–540 W), duration of ultrasound (10–50 min), ultrasonic temperature (10–60 °C), and the number of extractions (1–4) were obtained according to the single factor experiments. Then, using the Box-Behnken design (BBD) of response surface analysis, the optimum extraction conditions were obtained with 16.33% methanol concentration, the liquid-solid ratio of 10.83:1 mL/g and 35.00 °C ultrasonic temperature. Under these conditions, kinsenoside extraction yield reached 32.24% dry weight. The best conditions were applied to determine the kinsenoside content in seven different cultivation ages in Anoectochilus roxburghii.

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Optimization of Ultrasound-Assisted Extraction of Flavonoids from Celastrus hindsii Leaves Using Response Surface Methodology and Evaluation of Their Antioxidant and Antitumor Activities

BioMed Research International ◽

10.1155/2020/3497107 ◽

2020 ◽

Vol 2020 ◽

pp. 1-9 ◽

Cited By ~ 1

Author(s):

Dinh-Chuong Pham ◽

Hoang-Chinh Nguyen ◽

Thanh-Hang Le Nguyen ◽

Hoang-Linh Ho ◽

Thien-Kim Trinh ◽

...

Keyword(s):

Response Surface Methodology ◽

Response Surface ◽

Biological Activities ◽

Radical Scavenging ◽

Ultrasonic Power ◽

Extraction Temperature ◽

Anticancer Activities ◽

Ultrasound Assisted Extraction ◽

Assisted Extraction ◽

Ultrasound Assisted

Celastrus hindsii is a potential source of flavonoids with biological activities. This study aimed to develop an ultrasound-assisted technique for extracting flavonoids from leaves of C. hindsii. Response surface methodology was employed to optimize the extraction conditions for maximizing the total flavonoid content (TFC). A maximum TFC of 23.6 mg QE/g was obtained under the extraction conditions of ultrasonic power of 130 W, extraction temperature of 40°C, extraction time of 29 min, and ethanol concentration of 65%. The flavonoid-rich extracts were then studied for their antioxidant and anticancer activities. The results showed that the C. hindsii leaf extract exhibited potent radical scavenging activities against DPPH (IC50 of 164.85 μg/mL) and ABTS (IC50 of 89.05 μg/mL). The extract also significantly inhibited the growth of 3 cancer cell lines MCF7, A549, and HeLa with the IC50 values of 88.1 μg/mL, 120.4 μg/mL, and 118.4 μg/mL, respectively. Notably, the extract had no cytotoxicity effect on HK2 normal kidney cell line. This study suggests that flavonoid-rich extract is a promising antioxidant and anticancer agent and that ultrasound-assisted extraction is an efficient method for extracting flavonoids from C. hindsii leaves.

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Optimization of Ultrasound-Assisted Extraction Followed by Macroporous Resin Purification for Maximal Recovery of Functional Components and Removal of Toxic Components from Ginkgo biloba Leaves

BioMed Research International ◽

10.1155/2018/4598067 ◽

2018 ◽

Vol 2018 ◽

pp. 1-15

Author(s):

Guisheng Zhou ◽

Jiayan Ma ◽

Yuping Tang ◽

Xinmin Wang ◽

Jing Zhang ◽

...

Keyword(s):

Ginkgo Biloba ◽

Extraction Temperature ◽

Macroporous Resin ◽

Ultrasound Assisted Extraction ◽

Solid Ratio ◽

Assisted Extraction ◽

Column Adsorption ◽

Ultrasound Assisted ◽

Functional Components ◽

Toxic Components

In the present study, the process of ultrasound-assisted extraction (UAE) followed by macroporous resin purification was successfully developed to achieve maximal recovery of functional components (flavonoids and ginkgolides) with minimal contents of toxic components (alkylphenols) from Ginkgo biloba leaves. Three effective extracted factors including HAc%, EtOH%, and UAE power were screened by Plackett–Burman design (PBD). The important variables were further optimized by rotatable central composite design (RCCD). By combination of PBD and RCCD, the resulting optimal UAE conditions were as follows: HAc% of 1.8%, EtOH% of 63%, ultrasound power of 303 W, G. biloba leaves powder amount of 1.0 g (solvent-to-solid ratio 40 mL/g), particle size of 60 mesh, extraction time of 40 min, and extraction temperature of 45°C. Under the optimum conditions, the yield of flavonoids was 25.1 ± 0.81 mg/g, ginkgolides was 10.6 ± 0.57 mg/g, and alkylphenols was 3.96 ± 0.31 mg/g. Moreover, the further enriching the functional components and removing toxic components from the obtained extracts of G. biloba leaves using the above optimum UAE condition was successfully achieved by macroporous resin DA-201. After column adsorption and desorption on DA-201, the content of total flavonoids was 36.51 ± 1.53%, ginkgolides was 13.24 ± 0.85%, and alkylphenols was 7.0 ± 1.0 μg/g from the obtained dry extracts (drug to extract ratio of 45-50:1) of G. biloba leaves which were complied with Chinese pharmacopoeias.

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Response Surface Methodology (RSM)-Based Optimization of Ultrasound-Assisted Extraction of Sennoside A, Sennoside B, Aloe-Emodin, Emodin, and Chrysophanol from Senna alexandrina (Aerial Parts): HPLC-UV and Antioxidant Analysis

Molecules ◽

10.3390/molecules27010298 ◽

2022 ◽

Vol 27 (1) ◽

pp. 298

Author(s):

Perwez Alam ◽

Omar M. Noman ◽

Rashed N. Herqash ◽

Omer M. Almarfadi ◽

Ali Akhtar ◽

...

Keyword(s):

Response Surface Methodology ◽

Response Surface ◽

Extraction Time ◽

Extraction Temperature ◽

Ultrasound Assisted Extraction ◽

Solid Ratio ◽

Aerial Parts ◽

Assisted Extraction ◽

Aloe Emodin ◽

Ultrasound Assisted

In this study, ultrasound-assisted extraction conditions were optimized to maximize the yields of sennoside A, sennoside B, aloe-emodin, emodin, and chrysophanol from S. alexandrina (aerial parts). The three UAE factors, extraction temperature (S1), extraction time (S2), and liquid to solid ratio (S3), were optimized using response surface methodology (RSM). A Box–Behnken design was used for experimental design and phytoconstituent analysis was performed using high-performance liquid chromatography-UV. The optimal extraction conditions were found to be a 64.2 °C extraction temperature, 52.1 min extraction time, and 25.2 mL/g liquid to solid ratio. The experimental values of sennoside A, sennoside B, aloe-emodin, emodin, and chrysophanol (2.237, 12.792, 2.457, 0.261, and 1.529%, respectively) agreed with those predicted (2.152, 12.031, 2.331, 0.214, and 1.411%, respectively) by RSM models, thus demonstrating the appropriateness of the model used and the accomplishment of RSM in optimizing the extraction conditions. Excellent antioxidant properties were exhibited by S. alexandrina methanol extract obtained using the optimized extraction conditions with a DPPH assay (IC50 = 59.7 ± 1.93, µg/mL) and ABTS method (47.2 ± 1.40, µg/mL) compared to standard ascorbic acid.

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Optimization of Ultrasound-Assisted Extraction Condition for Phenolic Compounds, Antioxidant Activity, and Epigallocatechin Gallate in Lipid-Extracted Microalgae

Molecules ◽

10.3390/molecules25030454 ◽

2020 ◽

Vol 25 (3) ◽

pp. 454 ◽

Cited By ~ 6

Author(s):

Da Hye Gam ◽

Song Yi Kim ◽

Jin Woo Kim

Keyword(s):

Antioxidant Activity ◽

Phenolic Compounds ◽

Ethanol Concentration ◽

Epigallocatechin Gallate ◽

Statistical Optimization ◽

Ultrasonic Power ◽

Extraction Temperature ◽

Ultrasound Assisted Extraction ◽

Assisted Extraction ◽

Ultrasound Assisted

Lipid-extracted microalgae (LEM, Tetraselmis KCTC 12236BP), a solid waste by-product obtained from algal biodiesel production, is typically considered a rich source of antioxidant compounds, including phenolic compounds. The purpose of this study was to apply a statistically-based methodology to enhance the extraction of total phenolic compounds (TPCs) and antioxidant activity (AA) from LEM and to verify the production of epigallocatechin gallate (EGCG), a bioactive material, under optimum conditions. The optimal extractions of TPC and AA were explored by varying the key variables, including the extraction temperature, ethanol concentration, extraction time, and ultrasonic power, through statistical optimization. The optimal extraction conditions were identified through 27 runs following the central composite design. The regression analyses of TPC and AA showed good fit of the experimental data to the second-order polynomial models, with coefficient of determination (R2) values of 0.8769 and 0.8432, respectively. In the variation experiment, the maximum TPC and AA values of 9.8 mg GAE/g and 91.8% were obtained respectively with an extraction temperature of 74.4 °C, ethanol concentration of 55.4%, extraction time of 59.6 min, and ultrasonic power of 700 W. HPLC coupled with diode array detection was used to identify and quantify the phenolic compounds in the extracts, and EGCG (0.12 mg/g DM) was identified as a major peak in the analysis, demonstrating that high value-added material with a bioactive property can be produced from LEM. The results indicated that statistical optimization is applicable for optimizing the extraction of TPC and AA from LEM and provided a scientific basis for applying ultrasound-assisted extraction on an industrial scale by optimizing the conditions. LEM has a high TPC value, particularly with regard to EGCG, and excellent AA, considering it is highly used as a functional material for food, cosmetics, and medicine.

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