Effect of Ampicillin as Bacteriostats on the Performance of Phosphate Solubilizing Rhizobium. Meliloti Inoculants

2011 ◽  
Vol 201-203 ◽  
pp. 1023-1032
Author(s):  
Jian Feng Li ◽  
Shu Qing Zhang ◽  
Shang Li Shi ◽  
Ping Hui Huo ◽  
Li Yu Chen
Keyword(s):  
Calcium Phosphate ◽  
Plant Growth ◽  
Phosphate Solubilization ◽  
Rhizobium Meliloti ◽  
Growth Promotion ◽  
Nitrogenase Activity ◽  
Iaa Production ◽  
Viable Cells ◽  
Significant Difference ◽  
Phosphate Solubilizing

The aim of this study was to evaluate the effect of Ampicillin as bacteriostats on the number of P solubilizing and antibiotic-anti-Rhizobium. meliloticells and contamination in inoculants during one year storage, and plant promotion ability of subgeneration cells of antibiotic-resistant phosphate-solubilizing R. meliloti strain LW107 growth on YMA plates containing different concentrations of ampicillin were also investigated. In the first experiment, 100 mg liter-1 ampicillin was added in inoculants, number of R.meliloticells and contamination in inoculants were investigated at two temperatures, using both liquid and peat based solid inoculants in experiment. Statistical design was a complete randomized block in a factorial 2×2×2 experimental arrangement with 4 replicates. Results show that there are various viable cells and contamination levels in inoculants. The ampicillin increased R.melilotiLW107 viable cells and inoculants stored at low temperature with lesser contamination when ampicillin been added. To clarify whether ampicillin affect the main character of R.melilotiLW107 cells, the ability of calcium phosphate solubilization and IAA production in ampicillin-containing conditions was determined. Results indicated that the ability of calcium phosphate solubilization and IAA production have no significant difference on 6th generation cells of R.melilotiLW107 between the growth plates and the lack of ampicillin. In the third experiment, the plant growth promotion of the ampicillin-containing inoculants on alfalfa seedlings was determined in sterile sand conditions in a temperature-controlled growth chamber at 20 -25°C for 35 days. We also found that no significant difference on nodule numbers between diluted liquids ampicillin-containing inoculants and the common liquid inoculants containing no ampicillin, but plant growth and efficiency of nitrogen-fixing responded to undiluted liquids inoculants containing ampicillin with a decrease in biomass and nitrogenase activity indicating that the five-fold dilutions of the ampicillin-containing inoculants are necessary for applications.

scholarly journals SCREENING OF SOIL BACTERIA FOR PLANT GROWTH PROMOTION ACTIVITIES IN VITRO

2016 ◽  
Vol 4 (1) ◽  
pp. 27 ◽  
Author(s):  
Edi Husen
Keyword(s):  
Plant Growth ◽  
Soil Bacteria ◽  
Phosphate Solubilization ◽  
Azotobacter Vinelandii ◽  
Growth Promotion ◽  
Nitrogenase Activity ◽  
Chelating Agent ◽  
Plant Growth Promoting Rhizobacteria ◽  
Iaa Production

Fourteen isolates of soil bacteria, including two known plant growth promoting rhizobacteria (PGPR) strains, Azotobacter vinelandii Mac 259 and Bacillus cereus UW 85, were tested in vitro. Parameters assessed were indoleacetic acid (IAA) production, phosphate solubilization, dinitrogen fixation, and siderophore (Fe-III chelating agent) production. IAA production was assayed colorimetrically using ferric chlorideperchloric acid reagent. Phosphate-solubilization and siderophore production were tested qualitatively by plating the bacteria in Pikovskaya and chrome azurol S agar, respectively. The ability to fix dinitrogen was measured based on nitrogenase activity of the bacteria by gas chromatography. The results showed that twelve isolates produced IAA, ranged from 2.09 to 33.28 µmol ml-1. The ability to solubilize precipitated phosphate was positively exhibited by four isolates (BS 58, BTS, TCaR 61, and BTCaRe 65). Seven isolates including Mac 259 positively produced siderophore. None of the isolates showed nitrogenase activity. Only one isolate (TS 3) did not exhibit any of the traits tested. Isolate TCeRe 60 and reference strain Mac 259 were found to have IAA- and siderophore-producing traits. Four P-solubilizing bacteria (BS 58, BTS, TCaR 61, and BTCaRe 65) were also IAA- and siderophore-producing bacteria. Potential use of these PGPR isolates needs further test in enhancing plant growth.

scholarly journals SCREENING OF SOIL BACTERIA FOR PLANT GROWTH PROMOTION ACTIVITIES IN VITRO

2016 ◽  
Vol 4 (1) ◽  
pp. 27 ◽  
Author(s):  
Edi Husen
Keyword(s):  
Plant Growth ◽  
Soil Bacteria ◽  
Phosphate Solubilization ◽  
Azotobacter Vinelandii ◽  
Growth Promotion ◽  
Nitrogenase Activity ◽  
Chelating Agent ◽  
Plant Growth Promoting Rhizobacteria ◽  
Iaa Production

Fourteen isolates of soil bacteria, including two known plant growth promoting rhizobacteria (PGPR) strains, Azotobacter vinelandii Mac 259 and Bacillus cereus UW 85, were tested in vitro. Parameters assessed were indoleacetic acid (IAA) production, phosphate solubilization, dinitrogen fixation, and siderophore (Fe-III chelating agent) production. IAA production was assayed colorimetrically using ferric chlorideperchloric acid reagent. Phosphate-solubilization and siderophore production were tested qualitatively by plating the bacteria in Pikovskaya and chrome azurol S agar, respectively. The ability to fix dinitrogen was measured based on nitrogenase activity of the bacteria by gas chromatography. The results showed that twelve isolates produced IAA, ranged from 2.09 to 33.28 µmol ml-1. The ability to solubilize precipitated phosphate was positively exhibited by four isolates (BS 58, BTS, TCaR 61, and BTCaRe 65). Seven isolates including Mac 259 positively produced siderophore. None of the isolates showed nitrogenase activity. Only one isolate (TS 3) did not exhibit any of the traits tested. Isolate TCeRe 60 and reference strain Mac 259 were found to have IAA- and siderophore-producing traits. Four P-solubilizing bacteria (BS 58, BTS, TCaR 61, and BTCaRe 65) were also IAA- and siderophore-producing bacteria. Potential use of these PGPR isolates needs further test in enhancing plant growth.

scholarly journals Isolation and identification of phosphate solubilizing bacteria and evaluate its effect on of Mung bean (Vigna radita [L.] R.Wilczek) growth

2019 ◽  
Vol 60 (5) ◽  
pp. 985-995
Author(s):  
Yusur Ramzi ◽  
Hutaf A. A. Alsalim
Keyword(s):  
Plant Growth ◽  
Bacillus Cereus ◽  
Mung Bean ◽  
Phosphate Solubilization ◽  
Growth Promotion ◽  
Hydrolytic Enzymes ◽  
Phosphate Solubilizing Bacteria ◽  
Biochemical Tests ◽  
Isolation And Identification ◽  
Phosphate Solubilizing

Sixteen soil samples were collected from wheat, barley and yellow corn rhizosphere in Abu-Ghraib, Aqraqof, Latifieh,Tarmiah, Jadriya and  of Agriculture in Baghdad university/ Baghdad city. The results found nine phosphate solubilizing bacteria (PSB) isolates (Y1, Y2, Y3, Y4, Y5, Y6, Y7, Y8, Y9), formed clear zones on National Botanical Research Institute's (NBRIP) agar. The solubility index (SI) of PSB isolates ranged from 2.00 to 3.66. Y4 have the highest SI (3.66) followed by Y3 and Y6 (3.33). Phosphate solubilization abilities varying from (20.10-39.00 μg.ml-1), Y4 was the highest (39.00 μg.ml-1) followed by Y3 (37.00μg.ml-1). The results of hydrolytic enzymes production showed that almost all nine isolates are able to produce protease and pectinase, while Y1 and Y2 showed negative results in cellulase production. Maximum ability for hydrogen cyanide (HCN) and indole acetic acid (IAA) production were showed byY3 and Y4 isolates. The isolate Y4 was found to be the most efficient isolate, so it was selected identified as Bacillus cereus using biochemical tests confirmed by VITEC 2 compact system. The results of High performance liquid chromatography (HPLC) revealed that Bacillus cereus produce oxalic acid (2.996), citric acid (9.117) and malic acid (3.734). Bacillus cereus (Y4) enhanced the growth of mung bean plants. A significant increase in branches number (12.33), plant length (83.0cm), fresh weight (27.25 g) and dry weight (1.427g) were obtained compared with control treatments. The main objective of this study is to isolate PSB and evaluate their roles in plant growth promotion. The results showed the high phosphate solubilization efficiency of PSB isolates and the identified isolates was found to be good enough for plant growth promoting.

scholarly journals Effects of Talaromyces purpureogenus on Cucumber Growth Promotion and Its Mechanism

2021 ◽  
Vol 8 (3) ◽  
Author(s):  
Zhao L ◽  
◽  
Zhao W ◽  
Deng H ◽  
◽  
...  
Keyword(s):  
Plant Growth ◽  
Growth Promotion ◽  
Growth Parameters ◽  
Scientific Basis ◽  
Promoting Effect ◽  
Iaa Production ◽  
Significant Difference ◽  
Application Potential ◽  
Promote Plant Growth ◽  
Growth Promoting

Some fungi may promote plant growth by production of siderophores, Indole Acetic Acid (IAA) and phosphorus dissolving capability. In this study, eight fungi were isolated from the mushroom substrate, and their siderophores production, IAA production and phosphorus dissolving traits were determined. Although there was no significant difference in IAA production among the eight fungi, but the strain M13026-2 was a fungus with strong growth promoting traits compared with other seven fungi. In order to study the correlation between the growth promoting effect of cucumber pot culture and the above three traits, five fungi with different strength of traits were tested in pot. As a result, M13026- 2 which was identified as Talaromyces purpureogenus could significantly improve the growth parameters of cucumber seedlings, and could colonize in the rhizosphere soil and the tissue of cucumber stably. All the results suggested that the most relevant to their ability to promote plant growth is the trait of phosphorus dissolving, followed by siderophores production. The results of this study will provide scientific basis for the efficient selection and identification of a large number of fungi resources with the function of promoting plant growth, and reveal the good application potential of T. purpureogenus in agriculture fields.

Plant growth promoting traits shown by bacteria Brevibacterium frigrotolerans SMA23 Isolated from Aloe vera rhizosphere

2017 ◽  
Vol 37 (03) ◽  
Author(s):  
Meena . ◽  
Nayan Tara ◽  
Baljeet Singh Saharan
Keyword(s):  
Plant Growth ◽  
Plant Growth Promotion ◽  
Phosphate Solubilization ◽  
Growth Promotion ◽  
Aloe Vera ◽  
Maximum Growth ◽  
Iaa Production ◽  
Rrna Sequencing ◽  
Plant Growth Promoting ◽  
Growth Promoting

Brevibacterium frigrotolerans SMA23 is a gram positive rod shaped bacteria isolated from Aloe vera rhizosphere. 16S rRNA sequencing confirmed the identity of the bacterium as Brevibacterium frigrotolerans. It was capable of growing at temperatures ranging from 10°C to 35°C, but maximum growth was observed at 30°C. It is endowed with multiple plant growth promotion attributes such as phosphate solubilization, IAA production and siderophore production, which are expressed differentially at sub-optimal temperatures. At 10°C it was found to exhibit all the plant growth promotion attributes. This bacterial isolate was able to positively influence and promote the growth and nutrient uptake parameters of wheat (HD 2967) under glasshouse conditions.

scholarly journals Evaluation of Phytase Producing Bacteria for Their Plant Growth Promoting Activities

2014 ◽  
Vol 2014 ◽  
pp. 1-7 ◽  
Author(s):  
Prashant Singh ◽  
Vinod Kumar ◽  
Sanjeev Agrawal
Keyword(s):  
Plant Growth ◽  
Phosphate Solubilization ◽  
Growth Promotion ◽  
Growth Parameters ◽  
Growth Effect ◽  
Bacterial Isolates ◽  
Iaa Production ◽  
Plant Growth Promoting Activities ◽  
Plant Growth Promoting ◽  
Growth Promoting

Bacterial inoculants are known to possess plant growth promoting abilities and have potential as liquid biofertilizer application. Four phytase producing bacterial isolates (phytase activity in the range of 0.076–0.174 U/mL), identified asAdvenellaspecies (PB-05, PB-06, and PB-10) andCellulosimicrobiumsp. PB-09, were analyzed for their plant growth promoting activities like siderophore production, IAA production, HCN production, ammonia production, phosphate solubilization, and antifungal activity. All isolates were positive for the above characteristics except for HCN production. The solubilization index for phosphorus on Pikovskaya agar plates was in the range of 2–4. Significant amount of IAA (7.19 to 35.03 μg/mL) production and solubilized phosphate (189.53 to 746.84 μg/mL) was noticed by these isolates at different time intervals. Besides that, a greenhouse study was also conducted with Indian mustard to evaluate the potential of these isolates to promote plant growth. Effect of seed bacterization on various plant growth parameters and P uptake by plant were used as indicators. The plant growth promoting ability of bacterial isolates in pot experiments was correlated to IAA production, phosphate solubilization, and otherin vitrotests. On the basis of present findings, isolate PB-06 was most promising in plant growth promotion with multiple growth promoting characteristics.

scholarly journals Multifarious functional traits of free-living rhizospheric fungi, with special reference to Aspergillus spp. isolated from North Indian soil, and their inoculation effect on plant growth

2021 ◽  
Vol 71 (1) ◽  
Author(s):  
Mohd Imran ◽  
Hussein H. Abulreesh ◽  
Mohammad K. Monjed ◽  
Khaled Elbanna ◽  
Samreen ◽  
...  
Keyword(s):  
Plant Growth ◽  
Functional Traits ◽  
Extracellular Enzymes ◽  
Phosphate Solubilization ◽  
Growth Promotion ◽  
Extracellular Enzyme ◽  
Growth And Yield ◽  
Multiple Traits ◽  
Iaa Production ◽  
Fungal Isolates

Abstract Purpose Rhizospheric soil fungi are critical for plant and soil health. However, their multiple functional traits and impact on plant growth have not been systematically explored. Methods During this study, biochemical traits of 73 indigenous soil fungal isolates and 15 unidentified isolates related to plant growth promotion and production of extracellular enzymes were studied. Results Forty four (65.67%) of the total isolates produced indole acetic acid (IAA) followed by siderophore (52.23%), phosphate solubilization (37.31%), and antibiotic (11.93%). 91.04% of the studied isolates produced ammonia whereas 28.35% produced organic acid. Extracellular enzyme activities of lipase, amylase, chitinase, and cellulase were detected among 95.52%, 61.11%, 35.82%, and 41.79% isolates, respectively. Based on these activities, 73 fungal isolates were categorized into different biotypes. Quantitative analysis of IAA production and phosphate solubilization was carried out for Aspergillus, Penicillium, and Rhizopus isolates. Aspergillus isolates exhibited varying activities of IAA production and phosphate solubilization. Most of the Aspergillus isolates and some other fungi demonstrated multiple activities. Based on the multiple traits of selected fungal isolates, Aspergillus sp-07, Penicillium sp-03, and Rhizopus sp-02 were further evaluated in different combinations for their inoculation effect on the growth and yield of wheat under field conditions. Conclusions The results indicated that these isolates could be developed into bio-inoculants to enhance plant growth. The consortium of these three isolates was also found to be compatible and beneficial for plant growth.

Role of bacterial pyrroloquinoline quinone in phosphate solubilizing ability and in plant growth promotion on strain Serratia sp. S119

Symbiosis ◽  
2016 ◽  
Vol 72 (1) ◽  
pp. 31-43 ◽  
Author(s):  
Liliana Mercedes Ludueña ◽  
María Soledad Anzuay ◽  
Jorge Guillermo Angelini ◽  
Germán Barros ◽  
María Flavia Luna ◽  
...  
Keyword(s):  
Plant Growth ◽  
Plant Growth Promotion ◽  
Growth Promotion ◽  
Pyrroloquinoline Quinone ◽  
Phosphate Solubilizing
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