Use of ionic liquids for improvement of cellulosic ethanol production

Cellulosic ethanol production has drawn much attention in recent years. However, there remain significant technical challenges before such production can be considered as economically feasible at an industrial scale. Among them, the efficient conversion of carbohydrates in lignocellulosic biomass into fermentable sugars is one of the most challenging technical difficulties in cellulosic ethanol production. Use of ionic liquids has opened new avenues to solve this problem by two different pathways. One is pretreatment of lignocellulosic biomass using ionic liquids to increase its enzymatic hydrolysis efficiency. The other is to transform the hydrolysis process of lignocellulosic biomass from a heterogeneous reaction system to a homogeneous one by dissolving it into ionic liquids, thus improving its hydrolysis efficiency.

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Production of Cellulosic Ethanol from Enzymatically Hydrolysed Wheat Straws

Applied Sciences ◽

10.3390/app10217638 ◽

2020 ◽

Vol 10 (21) ◽

pp. 7638

Author(s):

Vasile-Florin Ursachi ◽

Gheorghe Gutt

Keyword(s):

Enzymatic Hydrolysis ◽

Wheat Straw ◽

Cellulosic Ethanol ◽

Raw Material ◽

High Yield ◽

Liquid Ratio ◽

Hydrolysis Process ◽

Pretreatment Conditions ◽

Pretreated Wheat Straw ◽

First Time

The aim of this study is to find the optimal pretreatment conditions and hydrolysis in order to obtain a high yield of bioethanol from wheat straw. The pretreatments were performed with different concentrations of sulphuric acid 1, 2 and 3% (v/v), and were followed by an enzymatic hydrolysis that was performed by varying the solid-to-liquid ratio (1/20, 1/25 and 1/30 g/mL) and the enzyme dose (30/30 µL/g, 60/60 µL/g and 90/90 µL/g Viscozyme® L/Celluclast® 1.5 L). This mix of enzymes was used for the first time in the hydrolysis process of wheat straws which was previously pretreated with dilute sulfuric acid. Scanning electron microscopy indicated significant differences in the structural composition of the samples because of the pretreatment with H2SO4 at different concentrations, and ATR-FTIR analysis highlighted the changes in the chemical composition in the pretreated wheat straw as compared to the untreated one. HPLC-RID was used to identify and quantify the carbohydrates content resulted from enzymatic hydrolysis to evaluate the potential of using wheat straws as a raw material for production of cellulosic ethanol in Romania. The highest degradation of lignocellulosic material was obtained in the case of pretreatment with 3% H2SO4 (v/v), a solid-to-liquid ratio of 1/30 and an enzyme dose of 90/90 µL/g. Simultaneous saccharification and fermentation were performed using Saccharomyces cerevisiae yeast, and for monitoring the fermentation process a BlueSens equipment was used provided with ethanol, O2 and CO2 cap sensors mounted on the fermentation flasks. The highest concentration of bioethanol was obtained after 48 h of fermentation and it reached 1.20% (v/v).

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Techno-Economic Implications of Fed-Batch Enzymatic Hydrolysis

Processes ◽

10.3390/pr7110847 ◽

2019 ◽

Vol 7 (11) ◽

pp. 847 ◽

Cited By ~ 1

Author(s):

Ellen Argo ◽

Deepak R. Keshwani

Keyword(s):

Enzymatic Hydrolysis ◽

Ethanol Production ◽

Process Simulation ◽

Cellulosic Ethanol ◽

Production Cost ◽

Simulation Software ◽

Labor Costs ◽

Fed Batch ◽

Capital Costs ◽

Plant Capacity

Fed-batch enzymatic hydrolysis has the potential to improve the overall process of converting cellulosic biomass into ethanol. This paper utilizes a process simulation approach to identify and quantify techno-economic differences between batch and fed-batch enzymatic hydrolysis in cellulosic ethanol production. The entire process of converting corn stover into ethanol was simulated using SuperPro Designer simulation software. The analysis was conducted for a plant capacity of 2000 metric tons of dry biomass per day. A literature review was used to identify baseline parameters for the process. The sensitivity of the ethanol production cost to changes in sugar conversion efficiency, plant capacity, biomass cost, power cost, labor cost, and enzyme cost was evaluated using the process simulation. For the base scenario, the ethanol unit production cost was approximately $0.10/gallon lower for fed-batch hydrolysis. The greatest differences were seen in facilities costs, labor costs, and capital costs. Using a fed-batch operation decreased facilities costs by 41%, labor costs by 21%, and capital costs by 15%. The sensitivity analysis found that cost of biomass had the greatest effect on ethanol production cost, and in general, the results support the proposition that fed-batch enzymatic hydrolysis does improve the techno-economics of cellulosic ethanol production.

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PRE-TREATMENT AND ENZYMATIC HYDROLYSIS OF BANANA (Musa acuminata x balbisiana) PSEUDOSTEM FOR ETHANOL PRODUCTION

Agro Bali: Agricultural Journal ◽

10.37637/ab.v3i2.608 ◽

2020 ◽

Vol 3 (2) ◽

pp. 98-107

Author(s):

Galileo E. Araguirang ◽

Arianne Joyce R. Arizala ◽

Eden Beth B. Asilo ◽

Jamie Louise S. Batalon ◽

Erin B. Bello ◽

...

Keyword(s):

Enzymatic Hydrolysis ◽

Ethanol Production ◽

Incubation Time ◽

Enzyme Concentration ◽

The Philippines ◽

Solid Loading ◽

Hydrolysis Process ◽

Pre Treatment ◽

Hydrolysis Of ◽

Second Generation Ethanol

Banana (M. acuminata x balbisiana) is an abundant lignocellulosic waste material in large plantations all over the Philippines, especially in Mindanao, which can be utilized as substrate in producing high-value products like ethanol. To compensate for the low yield based on total weight of substrate due to the high moisture content of banana pseudostem, there is the primary challenge to make the conversion of this lignocellulosic biomass into monomeric sugar and then into ethanol more efficiently in order to achieve yields that would make it cost-competitive. Hence, this study evaluated the effects of solid loading, incubation time and amount of enzyme on yield of reducing sugars in the enzymatic hydrolysis process and attempted to optimize the significant factors by Response Surface Methodology (RSM), specifically using Box-Behnken design. There was significant improvement on the reducing sugar yield of the pretreated banana pseudostem at 20 h incubation time, 15 g solid loading and 0.55 % enzyme concentration. Ethanol production was observed to be higher in the detoxified substrate although biomass was higher for the non-detoxified substrate. As to our knowledge, the present study is the first attempt to produce second generation ethanol using banana pseudostem waste as feedstock in the Philippines.

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Differences in chemical composition and physical properties imprinted by industrial storage on sugarcane bagasse result in its efficient enzymatic hydrolysis

Sustainable Energy & Fuels ◽

10.1039/d1se01240a ◽

2021 ◽

Author(s):

Vanessa de Oliveira Arnoldi Pellegrini ◽

Regiane P. Ratti ◽

Jefferson G Filgueiras ◽

Mauricio Falvo ◽

Marisa A.L. Coral ◽

...

Keyword(s):

Chemical Composition ◽

Enzymatic Hydrolysis ◽

Physical Properties ◽

Ethanol Production ◽

Sugarcane Bagasse ◽

Second Generation ◽

Biomass Pretreatment ◽

Fermentable Sugars ◽

Industrial Storage ◽

Second Generation Ethanol

Sugarcane bagasse (SCB) is a promising feedstock for second-generation ethanol production. Bioconversion of lignocellulose into fermentable sugars involve several technological steps, with biomass pretreatment being among the most expensive ones....

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Densifying lignocellulosic biomass with sulfuric acid provides a durable feedstock with high digestibility and high fermentability for cellulosic ethanol production

Renewable Energy ◽

10.1016/j.renene.2021.10.015 ◽

2021 ◽

Author(s):

Xinchuan Yuan ◽

Xiangxue Chen ◽

Guannan Shen ◽

Sitong Chen ◽

Jianming Yu ◽

...

Keyword(s):

Sulfuric Acid ◽

Ethanol Production ◽

Lignocellulosic Biomass ◽

Cellulosic Ethanol

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Feasibility of Bioethanol Production From Lignocellulosic Biomass

Scientific Journal of Riga Technical University Environmental and Climate Technologies ◽

10.2478/v10145-010-0011-x ◽

2010 ◽

Vol 4 (-1) ◽

pp. 11-15 ◽

Cited By ~ 1

Author(s):

Zane Aunina ◽

Gatis Bazbauers ◽

Karlis Valters

Keyword(s):

Ethanol Production ◽

Lignocellulosic Biomass ◽

Cellulosic Ethanol ◽

Theoretical Calculations ◽

Production Method ◽

Bioethanol Production ◽

Price Comparison ◽

Production Processes

Feasibility of Bioethanol Production From Lignocellulosic Biomass The objective of the paper is to discuss the potential of cellulosic ethanol production processes and compare them, to find the most appropriate production method for Latvia's situation, to perform theoretical calculations and to determine the potential ethanol price. In addition, price forecasts for future cellulosic and grain ethanol are compared. A feasibility estimate to determine the price of cellulosic ethanol in Latvia, if production were started in 2010, was made. The grain and cellulosic ethanol price comparison (future forecast) was made through to the year 2018.

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