Ultra Convenient Synthesis of Lanthanide Based Magnetic-Fluorescent Hydrogels for Multimodal Cellular Imaging

2011 ◽  
Vol 266 ◽  
pp. 118-121 ◽  
Author(s):  
Bing Di Chen ◽  
Yong Yong Li ◽  
Bing Bo Zhang ◽  
Bo Zhang ◽  
Yu Lian Wu ◽  
...  
Keyword(s):  
Optical Imaging ◽  
Room Temperature ◽  
Cellular Imaging ◽  
Resonance Imaging ◽  
Fluorescent Properties ◽  
Convenient Synthesis ◽  
Multimodal Mri ◽  
Europium Chelate ◽  
Magnetic Resonance Imaging Mri

A simple approach has been developed to synthesize lanthanide based multifunctional magnetic-fluorescent hydrogels for cellular imaging via MRI and optical imaging synchronously. The multifunctional hydrogels are prepared by covalently conjugating gadolinium and europium chelate with biocompatible chitosan. The room temperature photoluminescence (PL) spectrum shows that the hydrogels have a strong PL emission, which is characteristic of Eu3+ transitions from the excited 5D0 to 7FJ levels. Moreover, in vitro magnetic resonance imaging (MRI) analysis shows that the hydrogels exhibit powerful T1-weighted MRI signal in water. As a result, the hydrogels combine magnetic and fluorescent properties and can be expected to act as a promising multimodal MRI/optical imaging probe.

scholarly journals Bioresorbable optical sensor systems for monitoring of intracranial pressure and temperature

2019 ◽  
Vol 5 (7) ◽  
pp. eaaw1899 ◽  
Author(s):  
Jiho Shin ◽  
Zhonghe Liu ◽  
Wubin Bai ◽  
Yonghao Liu ◽  
Ying Yan ◽  
...  
Keyword(s):  
Optical Sensors ◽  
Diagnostic Information ◽  
Resonance Imaging ◽  
Optical Components ◽  
Continuous Measurements ◽  
Fabry Perot ◽  
Magnetic Resonance Imaging Mri ◽  
Two Dimensional Photonic Crystal

Continuous measurements of pressure and temperature within the intracranial, intraocular, and intravascular spaces provide essential diagnostic information for the treatment of traumatic brain injury, glaucoma, and cardiovascular diseases, respectively. Optical sensors are attractive because of their inherent compatibility with magnetic resonance imaging (MRI). Existing implantable optical components use permanent, nonresorbable materials that must be surgically extracted after use. Bioresorbable alternatives, introduced here, bypass this requirement, thereby eliminating the costs and risks of surgeries. Here, millimeter-scale bioresorbable Fabry-Perot interferometers and two dimensional photonic crystal structures enable precise, continuous measurements of pressure and temperature. Combined mechanical and optical simulations reveal the fundamental sensing mechanisms. In vitro studies and histopathological evaluations quantify the measurement accuracies, operational lifetimes, and biocompatibility of these systems. In vivo demonstrations establish clinically relevant performance attributes. The materials, device designs, and fabrication approaches outlined here establish broad foundational capabilities for diverse classes of bioresorbable optical sensors.

scholarly journals Magnetic Resonance Imaging Property of Doxorubicin-Loaded Gadolinium/13X Zeolite/Folic Acid Nanocomposite

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
S Ghaderi
Keyword(s):  
Magnetic Resonance Imaging ◽  
Folic Acid ◽  
Magnetic Resonance ◽  
Relaxation Times ◽  
A549 Cells ◽  
In Vitro Study ◽  
Resonance Imaging ◽  
13X Zeolite ◽  
Magnetic Resonance Imaging Mri

Abstract Background: Magnetic resonance imaging (MRI) using nanostructures has been a proper method for tumor targeting purposes. Different MRI nanomaterials, targeting agents and anticancer drugs have been used for targeting of tumors. Objectives: This study aims to consider the MRI property of doxorubicin (DOX)-loaded gadolinium/13X zeolite/folic acid (Gd3+/13X/FA) nanocomposite. Material and Methods: In this in vitro study, Gd3+/13X/FA/DOX nanocomposite was prepared and the X-ray diffraction, scanning electron microscopy and MTT assay were conducted to evaluate the physicochemical properties of the nanocomposite. MRI was performed at 25°C using a 1.5 T clinical system to determine the T1 relaxation times and subsequently, the T1 relaxivity. Results: The size of the nanocomposite was in the range of 80-200 nm. The nanocomposite without DOX loading (Gd3+/13X/FA) showed compatibility for A549 cells for all concentrations while DOX-loaded nanocomposite was toxic for 62% of the cells at the concentration of 0.4 mg/ml. The T1 relaxivity of Gd3+/13X/FA/DOX nanocomposite was 4.0401 mM-1s-1. Conclusion: Gd3+/13X/FA/DOX nanocomposite shows a T1 relaxivity similar to the conventional gadolinium chelates, and a successful DOX loading.

scholarly journals MCF-7, ACHN, and A549 Cancer Cells Characterized by Quantitative Magnetic Resonance Imaging In Vitro

2021 ◽  
Vol 12 (4) ◽  
pp. 5174-5186
Keyword(s):  
Magnetic Resonance Imaging ◽  
Magnetic Resonance ◽  
Cancer Cells ◽  
Relaxation Times ◽  
Resonance Imaging ◽  
T1 And T2 ◽  
Magnetic Resonance Imaging Mri ◽  
Mcf 7

To work with cancer cell cultures in vitro at 1.5 Tesla Magnetic Resonance Imaging (MRI), it was necessary to develop dedicated receiver coils. This device allowed to adjust the shape of the tested objects and thus improve the quality of imaging. One of the conditions for this new device was to increase the recorded signal level and reduce the distance between the tested object and the receiving elements of the coil. MCF7 (breast adenocarcinoma, Her-2 positive), ACHN (kidney cancer cells), and A549 (lung cancer cells) were characterized by using magnetic resonance imaging (MRI) in vitro. MRI measurements were performed using the clinical scanner with a 1.5 Tesla magnetic field. MCF-7, ACHN, and A549 cancer cells were characterized by T1 and T2 relaxation times. For MCF-7 cells, the relaxation times T1 and T2 were 2360 ± 12 ms and 116 ± 0.9 ms, respectively. For ACHN cells, the relaxation times T1 and T2 were 1354 ± 193 ms and 80 ± 9 ms, respectively. Values of T1 and T2 for A549 cancer cells cultures were 1527 ± 59 ms and 150 ± 8 ms, respectively. Once an accurate pulse protocol has been established and satisfactory reproducibility was obtained, the determination of relaxation times can be used as a tool to monitor cancer cell cultures using MRI in vitro based on the determination of changes in relaxation times.

scholarly journals Fluorinated PLGA-PEG-Mannose Nanoparticles for Tumor-Associated Macrophage Detection by Optical Imaging and MRI

2021 ◽  
Vol 8 ◽  
Author(s):  
Giorgia Zambito ◽  
Siyuan Deng ◽  
Joost Haeck ◽  
Natasa Gaspar ◽  
Uwe Himmelreich ◽  
...  
Keyword(s):  
Magnetic Resonance ◽  
Optical Imaging ◽  
Tumor Development ◽  
Mannose Receptor ◽  
The Body ◽  
Mri Contrast Agent ◽  
Therapeutic Interventions ◽  
Magnetic Resonance Imaging Mri

Tumor-associated macrophages (TAMs) promote cancer growth and metastasis, but their role in tumor development needs to be fully understood due to the dynamic changes of tumor microenvironment (TME). Here, we report an approach to visualize TAMs by optical imaging and by Fluorine-19 (19F) magnetic resonance imaging (MRI) that is largely applied to track immune cells in vivo. TAMs are targeted with PLGA-PEG-mannose nanoparticles (NPs) encapsulating perfluoro-15-crown-5-ether (PFCE) as MRI contrast agent. These particles are preferentially recognized and phagocytized by TAMs that overexpress the mannose receptor (MRC1/CD206). The PLGA-PEG-mannose NPs are not toxic and they were up-taken by macrophages as confirmed by in vitro confocal microscopy. At 48 h after intravenous injection of PLGA-PEG-mannose NPs, 4T1 xenograft mice were imaged and fluorine-19 nuclear magnetic resonance confirmed nanoparticle retention at the tumor site. Because of the lack of 19F background in the body, observed 19F signals are robust and exhibit an excellent degree of specificity. In vivo imaging of TAMs in the TME by 19F MRI opens the possibility for detection of cancer at earlier stage and for prompt therapeutic interventions in solid tumors.

scholarly journals Dual-Contrast Cellular Magnetic Resonance Imaging

2009 ◽  
Vol 8 (5) ◽  
pp. 7290.2009.00024 ◽  
Author(s):  
Rohan Dharmakumar ◽  
Zhouli Zhang ◽  
Ioannis Koktzoglou ◽  
Sotirios A. Tsaftaris ◽  
Debiao Li
Keyword(s):  
Magnetic Resonance Imaging ◽  
Magnetic Resonance ◽  
Three Dimensional ◽  
Negative Contrast ◽  
Cellular Imaging ◽  
Resonance Imaging ◽  
Specificity And Sensitivity ◽  
Contrast Magnetic Resonance ◽  
Imaging Research ◽  
Magnetic Resonance Imaging Mri

Negative contrast magnetic resonance imaging (MRI) methods using magnetic susceptibility shifting agents have become one of the most important approaches in cellular imaging research. However, visualizing and tracking labeled cells on the basis of negative contrast is often met with limited specificity and sensitivity. Here we report on a MRI method for cellular imaging that generates a new contrast with a distinct topology for identifying labeled cells that has the potential to significantly improve both the sensitivity and the specificity. Specifically, we show that low flip-angle steady-state free precession MRI can be used to generate fast three-dimensional images of tissue that can be rapidly processed to generate quantitative metrics enabling color overlays indicative of regions containing labeled cells. The technique substantially improves the ability of MRI for detecting labeled cells by overcoming the fundamental limits that currently plague negative contrast methods.

Novel Single Walled Carbon Nanotube Based Magnetic-Fluorescent Nanohybrids as Dual-Modal MRI/Optical Imaging Probes

2012 ◽  
Vol 476-478 ◽  
pp. 1134-1137
Author(s):  
Bing Di Chen ◽  
Tian Zhou ◽  
Bo Zhang ◽  
Ai Hua Yao
Keyword(s):  
Optical Imaging ◽  
Near Infrared ◽  
Layer By Layer ◽  
Fluorescent Properties ◽  
Single Walled Carbon ◽  
Gadolinium Chelate ◽  
Lbl Assembly ◽  
Imaging Probes ◽  
Magnetic Resonance Imaging Mri ◽  
Walled Carbon Nanotubes

Gadolinium chelate and Quantum Dots (QDs) decorated single walled carbon nanotubes (SWNTs) nanohybrids (SWNT-Gd-QDs) were designed for dual-modal cellular imaging via magnetic resonance imaging (MRI) and optical imaging. The nanohybrids were prepared via a simple and novel layer-by-layer (LBL) assembly in combination with covalent connection strategy. The SWNT-Gd-QDs nanohybrids showed a strong near-infrared photoluminescence (PL) emission at room temperature and enhanced T1-weighted and T2-weighted MRI signals in water. Thus, the nanohybrids combine magnetic and fluorescent properties and can serve as dual-modal MRI/optical imaging contrast agent.

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