Fabrication of Antheraea pernyi Silk Fibroin-Based Thermoresponsive Hydrogel Nanofibers for Colon Cancer Cell Culture

Antheraea pernyi silk fibroin (ASF)-based nanofibers have wide potential for biomaterial applications due to superior biocompatibility. It is not clear whether the ASF-based nanofibers scaffold can be used as an in vitro cancer cell culture platform. In the current study, we fabricated novel ASF-based thermoresponsive hydrogel nanofibers by aqueous electrospinning for colon cancer (LoVo) cells culture. ASF was reacted with allyl glycidyl ether (AGE) for the preparation of allyl silk fibroin (ASF-AGE), which provided the possibility of copolymerization with allyl monomer. The investigation of ASF-AGE structure by 1H NMR revealed that reactive allyl groups were successfully linked with ASF. ASF-based thermoresponsive hydrogel nanofibers (p (ASF-AGE-NIPAAm)) were successfully manufactured by aqueous electrospinning with the polymerization of ASF and N-isopropylacrylamide (NIPAAm). The p (ASF-AGE-NIPAAm) spinning solution showed good spinnability with the increase of polymerization time, and uniform nanofibers were formed at the polymerization time of 360 min. The obtained hydrogel nanofibers exhibited good thermoresponsive that the LCST was similar with PNIPAAm at about 32 °C, and good degradability in protease XIV PBS solution. In addition, the cytocompatibility of colon cancer (LoVo) cells cultured in hydrogel nanofibers was assessed. It was demonstrated that LoVo cells grown on hydrogel nanofibers showed improved cell adhesion, proliferation, and viability than those on hydrogel. The results suggest that the p (ASF-AGE-NIPAAm) hydrogel nanofibers have potential application in LoVo cells culture in vitro. This study demonstrates the feasibility of fabricating ASF-based nanofibers to culture LoVo cancer cells that can potentially be used as an in vitro cancer cell culture platform.

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Montmorillonite colloid plates with adsorbed cytochrome c: in vitro cytotoxic effect on colon cancer cell culture

Cancer Nanotechnology ◽

10.1186/s12645-021-00095-7 ◽

2021 ◽

Vol 12 (1) ◽

Author(s):

Svetlana H. Hristova ◽

Alexandar M. Zhivkov

Keyword(s):

Cell Culture ◽

Colon Cancer ◽

Cancer Cells ◽

Cancer Cell ◽

Aqueous Suspension ◽

Colon Cancer Cell ◽

Composite Particles ◽

Total Charge ◽

Colloid Particles

Abstract Background The apoptosis (a cascade of biochemical reactions leading to suicide of damaged biological cells) is blocked in the cancer cells because of impossibility of cytochrome c (cytC) go out from the mitochondria. However, the apoptosis can be started by introducing of exogenous cytC into cytoplasm using colloid particles as a protein carrier due to ability of the cancer cells to phagocytize extracellular particles with submicron size. Results The clay mineral montmorillonite (MM) were used to prepare aqueous suspension of protein/mineral composite particles by electrostatic adsorption of the positively charged cytC globules on the negatively charged MM colloid plates, and then added to colon cancel culture. The results shows out that separately cytC and MM have no effect but the composite cytC-MM particles kill 95% of the cancer cells after 96 h treatment using equine cytC which is 97% structurally identical with the human cytC. To reach this high cytotoxicity we have formulated requirements to: (a) bare colloid particles (electric charge, form and size), (b) conditions for protein adsorption (concentrations, pH, ionic strength), and (c) suspension with the composite particles (positive total charge and optimal concentration). Due to satisfying these requirements we have reached cytotoxicity which is 1/3 higher than the reached by other authors using different artificial particles. The cytotoxicity rapidly increases with concentration of the cytC-MM particles but further it shows tendency to saturation. Methods The optimal pH 6.5 and the 10:3 mg/mg cytC/MM concentration ratio at adsorption were found out by employing computer (protein electrostatics) and physicochemical methods (microelectrophoresis and colloid electrooptics) to prepare cytC-MM suspension. The anticancer capability of cytC-MM nanoplates were investigated using cell culture of metastasizing colon cancer. Conclusion The in vitro experiments with colon cancer cell culture disclose that cytC-MM composite particles have potential for application in anticancer therapy of superficial neoplasms of the skin and the alimentary system (mouth cavity, esophagus, stomach, jejunum and colon). Graphic abstract

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In Vitro Study of Colon Cancer Cell Migration Using E‐Jet 3D Printed Cell Culture Platforms

Macromolecular Bioscience ◽

10.1002/mabi.201800205 ◽

2018 ◽

Vol 18 (11) ◽

pp. 1800205 ◽

Cited By ~ 2

Author(s):

Juchang Zhong ◽

Yingjie Zhang ◽

Jingfei Chen ◽

Ruiying Huang ◽

Yikun Yang ◽

...

Keyword(s):

Cell Culture ◽

Colon Cancer ◽

Cell Migration ◽

Cancer Cell ◽

In Vitro Study ◽

Colon Cancer Cell ◽

Vitro Study ◽

Cancer Cell Migration ◽

3D Printed

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CXCL2-CXCR2 axis mediates αV integrin-dependent peritoneal metastasis of colon cancer cells

Clinical & Experimental Metastasis ◽

10.1007/s10585-021-10103-0 ◽

2021 ◽

Author(s):

Mattias Lepsenyi ◽

Nader Algethami ◽

Amr A. Al-Haidari ◽

Anwar Algaber ◽

Ingvar Syk ◽

...

Keyword(s):

Colon Cancer ◽

Cell Adhesion ◽

Cancer Cells ◽

Cancer Cell ◽

Colon Cancer Cell ◽

Colon Cancer Cells ◽

Cancer Cell Adhesion ◽

And Migration ◽

Cxcr2 Antagonist

AbstractPeritoneal metastasis is an insidious aspect of colorectal cancer. The aim of the present study was to define mechanisms regulating colon cancer cell adhesion and spread to peritoneal wounds after abdominal surgery. Mice was laparotomized and injected intraperitoneally with CT-26 colon carcinoma cells and metastatic noduli in the peritoneal cavity was quantified after treatment with a CXCR2 antagonist or integrin-αV-antibody. CT-26 cells expressed cell surface chemokine receptors CXCR2, CXCR3, CXCR4 and CXCR5. Stimulation with the CXCR2 ligand, CXCL2, dose-dependently increased proliferation and migration of CT-26 cells in vitro. The CXCR2 antagonist, SB225002, dose-dependently decreased CXCL2-induced proliferation and migration of colon cancer cells in vitro. Intraperitoneal administration of CT-26 colon cancer cells resulted in wide-spread growth of metastatic nodules at the peritoneal surface of laparotomized animals. Laparotomy increased gene expression of CXCL2 at the incisional line. Pretreatment with CXCR2 antagonist reduced metastatic nodules by 70%. Moreover, stimulation with CXCL2 increased CT-26 cell adhesion to extracellular matrix (ECM) proteins in a CXCR2-dependent manner. CT-26 cells expressed the αV, β1 and β3 integrin subunits and immunoneutralization of αV abolished CXCL2-triggered adhesion of CT-26 to vitronectin, fibronectin and fibrinogen. Finally, inhibition of the αV integrin significantly attenuated the number of carcinomatosis nodules by 69% in laparotomized mice. These results were validated by use of the human colon cancer cell line HT-29 in vitro. Our data show that colon cancer cell adhesion and growth on peritoneal wound sites is mediated by a CXCL2-CXCR2 signaling axis and αV integrin-dependent adhesion to ECM proteins.

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Self-Assembling Polypeptide Hydrogels as a Platform to Recapitulate the Tumor Microenvironment

Cancers ◽

10.3390/cancers13133286 ◽

2021 ◽

Vol 13 (13) ◽

pp. 3286

Author(s):

Dariusz Lachowski ◽

Carlos Matellan ◽

Ernesto Cortes ◽

Alberto Saiani ◽

Aline F. Miller ◽

...

Keyword(s):

Cell Culture ◽

Tumor Microenvironment ◽

Cancer Cell ◽

Critical Role ◽

Hypoxia Inducible Factor ◽

Pancreatic Cancer Cell Line ◽

Cancer Cell Line ◽

Culture Systems ◽

A Cell

The tumor microenvironment plays a critical role in modulating cancer cell migration, metabolism, and malignancy, thus, highlighting the need to develop in vitro culture systems that can recapitulate its abnormal properties. While a variety of stiffness-tunable biomaterials, reviewed here, have been developed to mimic the rigidity of the tumor extracellular matrix, culture systems that can recapitulate the broader extracellular context of the tumor microenvironment (including pH and temperature) remain comparably unexplored, partially due to the difficulty in independently tuning these parameters. Here, we investigate a self-assembled polypeptide network hydrogel as a cell culture platform and demonstrate that the culture parameters, including the substrate stiffness, extracellular pH and temperature, can be independently controlled. We then use this biomaterial as a cell culture substrate to assess the effect of stiffness, pH and temperature on Suit2 cells, a pancreatic cancer cell line, and demonstrate that these microenvironmental factors can regulate two critical transcription factors in cancer: yes-associated protein 1 (YAP) and hypoxia inducible factor (HIF-1A).

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In vitro antiproliferative activity against human colon cancer cell lines of representative 4-thiazolidinones. Part I

Bioorganic & Medicinal Chemistry Letters ◽

10.1016/j.bmcl.2005.05.093 ◽

2005 ◽

Vol 15 (17) ◽

pp. 3930-3933 ◽

Cited By ~ 92

Author(s):

Rosaria Ottanà ◽

Stefania Carotti ◽

Rosanna Maccari ◽

Ida Landini ◽

Giuseppa Chiricosta ◽

...

Keyword(s):

Colon Cancer ◽

Cell Lines ◽

Cancer Cell ◽

Antiproliferative Activity ◽

Human Colon ◽

Colon Cancer Cell ◽

Human Colon Cancer Cell ◽

Human Colon Cancer ◽

Colon Cancer Cell Lines

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Delivery of functional exogenous proteins by plant-derived vesicles to human cells in vitro

Scientific Reports ◽

10.1038/s41598-021-85833-y ◽

2021 ◽

Vol 11 (1) ◽

Author(s):

Luiza Garaeva ◽

Roman Kamyshinsky ◽

Yury Kil ◽

Elena Varfolomeeva ◽

Nikolai Verlov ◽

...

Keyword(s):

Cell Culture ◽

Colon Cancer ◽

Extracellular Vesicles ◽

Mononuclear Cells ◽

Human Peripheral Blood ◽

Human Cells ◽

Bioactive Molecules ◽

Native Plant ◽

Peripheral Blood Mononuclear

AbstractPlant-derived extracellular vesicles (EVs) gain more and more attention as promising carriers of exogenous bioactive molecules to the human cells. Derived from various edible sources, these EVs are remarkably biocompatible, biodegradable and highly abundant from plants. In this work, EVs from grapefruit juice were isolated by differential centrifugation followed by characterization of their size, quantity and morphology by nanoparticle tracking analysis, dynamic light scattering, atomic force microscopy and cryo-electron microscopy (Cryo-EM). In Cryo-EM experiments, we visualized grapefruit EVs with the average size of 41 ± 13 nm, confirmed their round-shaped morphology and estimated the thickness of their lipid bilayer as 5.3 ± 0.8 nm. Further, using cell culture models, we have successfully demonstrated that native grapefruit-derived extracellular vesicles (GF-EVs) are highly efficient carriers for the delivery of the exogenous Alexa Fluor 647 labeled bovine serum albumin (BSA) and heat shock protein 70 (HSP70) into both human peripheral blood mononuclear cells and colon cancer cells. Interestingly, loading to plant EVs significantly ameliorated the uptake of exogenous proteins by human cells compared to the same proteins without EVs. Most importantly, we have confirmed the functional activity of human recombinant HSP70 in the colon cancer cell culture upon delivery by GF-EVs. Analysis of the biodistribution of GF-EVs loaded with 125I-labeled BSA in mice demonstrated a significant uptake of the grapefruit-derived extracellular vesicles by the majority of organs. The results of our study indicate that native plant EVs might be safe and effective carriers of exogenous proteins into human cells.

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Antitumour activity of XR5944 in vitro and in vivo in combination with 5-fluorouracil and irinotecan in colon cancer cell lines

British Journal of Cancer ◽

10.1038/sj.bjc.6602403 ◽

2005 ◽

Vol 92 (4) ◽

pp. 722-728 ◽

Cited By ~ 17

Author(s):

S M Harris ◽

P Mistry ◽

C Freathy ◽

J L Brown ◽

P A Charlton

Keyword(s):

Colon Cancer ◽

Cell Lines ◽

Cancer Cell ◽

Antitumour Activity ◽

Cancer Cell Lines ◽

Colon Cancer Cell ◽

Colon Cancer Cell Lines

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Isolation, Synthesis and Biological Evaluation of Phenylpropanoids from the Rhizomes of Alpania galanga

Natural Product Communications ◽

10.1177/1934578x1300801222 ◽

2013 ◽

Vol 8 (12) ◽

pp. 1934578X1300801 ◽

Cited By ~ 1

Author(s):

Sumit S Chourasiya ◽

Eppakayala Sreedhar ◽

K. Suresh Babu ◽

Nagula Shankaraiah ◽

V. Lakshma Nayak ◽

...

Keyword(s):

Lung Cancer ◽

Colon Cancer ◽

Anticancer Activity ◽

Cell Lines ◽

Cancer Cell ◽

Kinetic Resolution ◽

Human Cancer ◽

Cancer Cell Lines ◽

Biological Evaluation

Bioactivity guided investigation of the DCM: MeOH (1:1) extract from the rhizomes of Alpinia galanga led to the isolation of phenylpropanoids (1–9) and their structures were established by 1H NMR, 13C NMR, IR and LC-MS/MS. These compounds have been evaluated for their in vitro anticancer activity against the human cancer cell lines A549 (lung cancer), Colo-205 (colon cancer), A431 (skin cancer), NCI H460 (lung cancer), PC-3 (prostate cancer), and HT-29 (colon cancer). Compounds 4 and 9 showed potent anticancer activity (ranging from 1.3–19.7 μg/mL) against all the tested cancer cell lines. In addition, an asymmetric synthesis of acetoxychavicol acetate (1) and trans-p-coumaryl alcohol (4) has been accomplished in six steps starting from readily available p-hydroxybenzaldehyde for the first time. Grignard reaction and Sharpless kinetic resolution reactions were utilized as the key steps to install the basic core.

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