Self-Assembling Poly(ethylene glycol)/Poly-γ-glutamic Acid Nanoparticles for Targeted Drug Delivery and Plasmid DNA

2013 ◽  
Vol 721 ◽  
pp. 105-108
Author(s):  
Yang Ge ◽  
Ke Shuai Lu ◽  
Xue Yan Su
Keyword(s):  
Drug Delivery ◽  
Digital Processing ◽  
Spherical Particles ◽  
Aqueous Environment ◽  
Force Microscopy ◽  
Feeding Experiments ◽  
Self Assembling ◽  
Intracellular Drug Delivery ◽  
Transmission Electron ◽  
Poly Ethylene

These stable self-assembled nanoparticles were characterized by dynamic light scattering, transmission electron microscopy, and atomic force microscopy,which demonstrated that the nanosystem consists of spherical particles with a smooth surface both in aqueous environment and in dried state. Toxicity measurements showed that the composition is nontoxic when tested either on cell cultures or in animal feeding experiments. To evaluate the potential of the nanosystem for intracellular drug delivery and gene, the nanoparticles were fluorescently labeled and folic acid was attached as a cancer cell-specific targeting moiety. The quantitative data obtained by digital processing of the intensity of green color of each pixel in the pictures inside the cell boundaries and total intensity of fluorescence inside the cells showed thattargeted particles internalized into the cells significantly faster and the total accumulation of these particles was substantially higher in the cancer cells.

scholarly journals Poloxamer Hydrogels for Biomedical Applications

Pharmaceutics ◽  
2019 ◽  
Vol 11 (12) ◽  
pp. 671 ◽  
Author(s):  
Eleonora Russo ◽  
Carla Villa
Keyword(s):  
Drug Delivery ◽  
Ethylene Oxide ◽  
Propylene Oxide ◽  
Biomedical Application ◽  
Aqueous Environment ◽  
Molecular Weights ◽  
Long Time ◽  
Poly Ethylene Oxide ◽  
Poly Ethylene ◽  
Concentration Threshold

This review article focuses on thermoresponsive hydrogels consisting of poloxamers which are of high interest for biomedical application especially in drug delivery for ophthalmic, injectable, transdermal, and vaginal administration. These hydrogels remain fluid at room temperature but become more viscous gel once they are exposed to body temperature. In this way, the gelling system remains at the topical level for a long time and the drug release is controlled and prolonged. Poloxamers are synthetic triblock copolymers of poly(ethylene oxide)-b-poly(propylene oxide)-b-poly(ethylene oxide) (PEO-PPO-PEO), also commercially known as Pluronics®, Synperonics® or Lutrol®. The different poloxamers cover a range of liquids, pastes, and solids, with molecular weights and ethylene oxide–propylene oxide weight ratios varying from 1100 to 14,000 and 1:9 to 8:2, respectively. Concentrated aqueous solutions of poloxamers form thermoreversible gels. In recent years this type of gel has arouse interest for tissue engineering. Finally, the use of poloxamers as biosurfactants is evaluated since they are able to form micelles in an aqueous environment above a concentration threshold known as critical micelle concentration (CMC). This property is exploited for drug delivery and different therapeutic applications.

Redox Sensitive Self-Assembling Dipeptide for Sustained Intracellular Drug Delivery

2019 ◽  
Vol 30 (9) ◽  
pp. 2458-2468 ◽  
Author(s):  
Sameer Dhawan ◽  
Sukanya Ghosh ◽  
R. Ravinder ◽  
Sachendra S. Bais ◽  
Soumen Basak ◽  
...  
Keyword(s):  
Drug Delivery ◽  
Self Assembling ◽  
Intracellular Drug Delivery

scholarly journals Composite Nanogels Based on Zeolite-Poly(ethylene glycol) Diacrylate for Controlled Drug Delivery

Nanomaterials ◽  
2020 ◽  
Vol 10 (2) ◽  
pp. 195 ◽  
Author(s):  
Catalina Paula Spatarelu ◽  
Anita-Laura (Radu) Chiriac ◽  
Bogdan Cursaru ◽  
Tanta-Verona Iordache ◽  
Ana-Mihaela Gavrila ◽  
...  
Keyword(s):  
Drug Delivery ◽  
Ethylene Glycol ◽  
Natural Zeolite ◽  
Drug Loading ◽  
Controlled Drug Delivery ◽  
Poly Ethylene Glycol ◽  
Glycol Diacrylate ◽  
Preparation Conditions ◽  
Transmission Electron ◽  
Poly Ethylene

This study presents the design of novel composites nanogels, based on poly(ethylene glycol) diacrylate and natural zeolite particles, that are able to act as materials with controlled drug delivery properties. Natural zeolite–nanogels composite, with varying zeolite contents, were obtained by an inverse mini-emulsion technique and loaded with 5-fluorouracil, a widely used chemotherapeutic drug. Herein, the possibility of adjusting final properties by means of modifying the preparation conditions was investigated. The prepared composite nanogels are characterized by dynamic light scattering (DLS), transmission electron microscopy (TEM), Fourier transform infrared spectroscopy (FTIR), and thermogravimetric analysis (TGA). In light of this tunable drug-loading capability, swelling behaviour, and cytotoxicity, these composite nanogels could be highly attractive as drug reservoirs.

Microfluidic Synthesis of Lipid-Polymer Hybrid Nanoparticles for Targeted Drug Delivery

MRS Advances ◽  
2016 ◽  
Vol 1 (29) ◽  
pp. 2155-2160
Author(s):  
Eri A. Takami ◽  
Folarin Erogbogbo
Keyword(s):  
Drug Delivery ◽  
Low Cost ◽  
Three Dimensional ◽  
Hybrid Nanoparticles ◽  
Dimensional Structure ◽  
Force Microscopy ◽  
Polymer Hybrid ◽  
Medical Issues ◽  
Transmission Electron ◽  
Size And Morphology

ABSTRACTLipid-polymer hybrid nanoparticles (LPHN) have great potential as drug delivery devices for treatment of serious medical issues such as cardiovascular disease, tuberculosis, and cancer. Nanoprecipitation is a commonly used method to synthesize LPHN in a low cost manner. However, this multi-step process proves to be difficult in consistently producing uniformly sized nanoparticles. Here we developed a microfluidic device that utilizes a three-channel pathway and mixer channel to synthesize uniformly sized LPHN in a controlled manner. Dynamic light scattering results of the microfluidic synthesized nanoparticles show decrease in diameter size from 140 nm to 40 nm as the Reynolds number of the channel inflow increases. Transmission electron microscopy confirms the size and morphology of the nanoparticles. Three dimensional structure of the LPHN were observed using atomic force microscopy. The production of higher quality nanoparticles using our microfluidics device can expedite the research and development process of drug delivering lipid polymer nanoparticles.

Preparation and Time Resolved Photoluminescence of Nanoscale InP Islands in In0.48Ga0.52P

1995 ◽  
Vol 379 ◽  
Author(s):  
K. Eberl ◽  
A. Kurtenbach ◽  
K. HÄusler ◽  
F. Noll ◽  
W.W. RÜhle
Keyword(s):  
Lattice Mismatch ◽  
Excitation Power ◽  
Buffer Layers ◽  
Time Resolved ◽  
Force Microscopy ◽  
Self Assembling ◽  
Atomic Force ◽  
Transmission Electron ◽  
Decay Times ◽  
Time Resolved Photoluminescence

ABSTRACTNanoscale InP islands are formed during InP/In0 48Ga0.52P heteroepitaxy due to the lattice mismatch of about 3.7%. The samples are prepared by solid source molecular beam epitaxy on (001) GaAs substrate. Atomic force microscopy measurements show that the size of the islands is typically 15 to 50 nm in diameter and about 5 to 10 nm high depending on the nominally deposited InP layer thickness, which is between 1 and 7.5 monolayers. Transmission electron micrographs show the coherent incorporation into the In0.48Ga0.52P matrix for InP islands with 2.5 monolayers. Resonantly excited time-resolved photoluminescence (PL) measurements of the self assembling InP dots are performed for optical characterisation. The decay times are typically 400 ps. The dependence on excitation power and temperature indicates the quantum dot nature of the InP islands. Finally a pronounced alignment of the InP islands is obtained on strained In0.61Ga0.39P buffer layers.

Copolymer Nanoparticles Prepared by Self-Assembly of Poly(ethylene glycol) and Poly-γ-Glutamic Acid and its Characteristics

2013 ◽  
Vol 662 ◽  
pp. 136-139
Author(s):  
Ge Yang ◽  
Ke Shuai Lu ◽  
Xue Yan Su
Keyword(s):  
Ethylene Glycol ◽  
Glutamic Acid ◽  
Size Distribution ◽  
Self Assembly ◽  
Aqueous Media ◽  
Poly Ethylene Glycol ◽  
Polyelectrolyte Complexes ◽  
Self Assembling ◽  
Transmission Electron ◽  
Poly Ethylene

The paper describes the preparation and characterization of novel biodegradable nanoparticles based on self-assembly of poly-gamma-glutamic acid (γ-PGA) and poly(ethylene glycol) (PEG). The nanosystems were stable in aqueous media at low pH conditions. Solubility of the systems was determined by turbidity measurements. The particle size and the size distribution of the polyelectrolyte complexes were identified by dynamic lightscattering and transmission electron microscopy.It was found that the size and size distribution of the nanosystems depends on the concentrations of γ-PGA and PEG solutions and their ratio as well as on the pH of the mixture and the order of addition. The diameter of individual particles was in the range of 30–270 nm. measured by TEM, and the average hydrodynamic diameters were between 130 and 300 nm. These biodegradable, self-assembling stable nanocomplexes might be useful for several biomedical applications.

scholarly journals Dual-responsive Gemini Micelles for Efficient Delivery of Anticancer Therapeutics

Polymers ◽  
2019 ◽  
Vol 11 (4) ◽  
pp. 604 ◽  
Author(s):  
Young Choi ◽  
Eun-sook Choi ◽  
Kwan Mun ◽  
Se Lee ◽  
Sung Lee ◽  
...  
Keyword(s):  
Drug Delivery ◽  
Polymeric Micelles ◽  
Therapeutic Effects ◽  
Cancer Therapies ◽  
Drug Delivery Vehicles ◽  
Dual Responsive ◽  
Intracellular Drug Delivery ◽  
Efficient Delivery ◽  
Poly Ethylene ◽  
Oxidation Of Methionine

Polymeric micelles as drug delivery vehicles are popular owing to several advantages. In this study, a gemini amphiphile (gemini mPEG-Cys-PMT) consisting of hydrophilic poly(ethylene glycol) and hydrophobic poly(methionine) with cystine disulfide spacer was synthesized and its micellar properties for thiol- or reactive oxygen species (ROS)-dependent intracellular drug delivery were described. The cleavage of cystine linkage in a redox environment or the oxidation of methionine units in a ROS environment caused the destabilization of micelles. Such redox- or ROS-triggered micellar destabilization led to enhanced release of encapsulated doxorubicin (DOX) to induce cytotoxicity against cancer cells. Further, the therapeutic effects of the DOX-loaded micelles were demonstrated using the KB cell line. This study shows that thiol and ROS dual-responsive gemini micelles are promising platforms for nano-drug delivery in various cancer therapies.

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