A Novel Extraction Method for Active Compounds from Traditional Chinese Medicinal Materials Environment Friendly

2013 ◽  
Vol 726-731 ◽  
pp. 618-621 ◽  
Author(s):  
Qing Shan Liu ◽  
Li Na Yi ◽  
Ke Qin Li ◽  
Xiao Ying Yin
Keyword(s):  
Molecularly Imprinted Polymers ◽  
Extraction Method ◽  
Solid Phase ◽  
Extraction Methods ◽  
Template Molecule ◽  
Environment Friendly ◽  
Active Compounds ◽  
Picroside Ii ◽  
Scatchard Equation ◽  
Novel Method

A novel extraction method based on environmental protection was founded through molecularly imprinted polymers (MIPs), overcoming the defects of traditional extraction methods, such as reagents consuming, complex operation and environmental pollution. MIPs were prepared by precipitation polymerization with picroside II or ginsenoside rg1 as the template molecule. The morphology of MIPs was characterized by scanning electron microscope and its static adsorption capacity was measured by the Scatchard equation. In addition, MIPs were filled in the solid phase extraction (SPE) column to separate and enrich the template molecule and its analogues, compared with C18-SPE column. The results indicated that MIPs have high affinity toward the template molecules, which might offer a novel method for the extraction of active compounds in the traditional herbal medicine.

Practice and Synthetize Molecularly Imprinted Polymers for Seperating Target Neuro-Protective Compounds from TCM with Less Pollution

2014 ◽  
Vol 955-959 ◽  
pp. 239-242
Author(s):  
Ping Geng ◽  
Qing Shan Liu ◽  
Kebaituli Gulibanumu ◽  
Xu Li ◽  
Ke Qin Li ◽  
...  
Keyword(s):  
Electron Microscope ◽  
Molecularly Imprinted Polymers ◽  
Extraction Method ◽  
Template Molecule ◽  
Ginsenoside Rb1 ◽  
Molecularly Imprinted ◽  
Imprinted Polymers ◽  
Synthetic Materials ◽  
Scatchard Equation ◽  
Environmentally Friendly Method

Molecularly imprinted polymers (MIPs) are synthetic materials that can be the environmental protection extraction method in TCM research and industry. They can overcome the defects of traditional extract methods and environmental pollution. In our research, MIPs were prepared by precipitation polymerization with neuro-protective picroside I and ginsenoside Rb1 as the template molecule. Moreover, the morphology of MIPs was characterized by electron microscope scanning and the static adsorption capacity was measured by the Scatchard equation. Finally, MIPs were made into MIP-SPE columns to enrich the template molecule and its analogues comparing with C18-SPE column and the results show that MIPs have good affinity and selectivity towards the Rb1 and Picroside I in SPE columns. This research may offer a more environmentally friendly method to extract active compounds in the traditional herbal.

A Novel Method for Prepairing Higher Performance Picroside I Surface Molecularly Imprinted Polymers for TCM Research

2013 ◽  
Vol 785-786 ◽  
pp. 642-645
Author(s):  
Qing Shan Liu ◽  
Ke Qin Li ◽  
Jun Li ◽  
Xiao Ying Yin ◽  
Tian Hua Yan
Keyword(s):  
Electron Microscope ◽  
Molecularly Imprinted Polymers ◽  
Template Molecule ◽  
Functional Monomer ◽  
Molecular Imprinted Polymers ◽  
Molecularly Imprinted ◽  
Imprinted Polymers ◽  
Scatchard Equation ◽  
Novel Method ◽  
Scanning Electron

To establish a novel method for preparing molecularly imprinted polymers for Picroside I with better performance on TCM research contrast to previous studies, we have prepared novel surface molecular imprinted polymers (S-MIPs) using Picroside I as the template molecule, Acrylamide (AM) as the functional monomer, and silica gel as the carrier. The morphology of S-MIPs was characterized by scanning electron microscope (SEM) and its static adsorption capacity was measured by the Scatchard equation.

A Novel Method for Preparing the Surface Molecularly Imprinted Polymers to Target Isolate Ginsenoside Rg1 and its Analogues

2012 ◽  
Vol 535-537 ◽  
pp. 2400-2403 ◽  
Author(s):  
Qing Shan Liu ◽  
Li Na Yi ◽  
Qiu Juan Wang ◽  
Qing Long Guo ◽  
Yi Fan Jiang ◽  
...  
Keyword(s):  
Molecularly Imprinted Polymers ◽  
Template Molecule ◽  
Functional Monomer ◽  
Ginsenoside Rg1 ◽  
Molecular Imprinted Polymers ◽  
Molecularly Imprinted ◽  
Imprinted Polymers ◽  
Scatchard Equation ◽  
Novel Method ◽  
Scanning Electron

To establish a novel method for preparing molecularly imprinted polymers for ginsenoside Rg1 with better character contrast to previous studies, we have prepared novel surface molecular imprinted polymers (S-MIPs) using ginsenoside Rg1 as the template molecule, Acrylamide (AM) as the functional monomer, and silica gel as the carrier. The morphology of S-MIPs was characterized by scanning electron microscope (SEM) and its static adsorption capacity was measured by the Scatchard equation.

Preparation and Properties of Ginsenoside Rg1 Molecularly Imprinted Polymers

2012 ◽  
Vol 550-553 ◽  
pp. 1715-1718 ◽  
Author(s):  
Li Na Yi ◽  
Xiao Ying Yin ◽  
Yi Fan Jiang ◽  
Qing Shan Liu
Keyword(s):  
Molecularly Imprinted Polymers ◽  
Scatchard Analysis ◽  
Template Molecule ◽  
Ginsenoside Rg1 ◽  
Traditional Herbal Medicine ◽  
Molecularly Imprinted ◽  
Imprinted Polymers ◽  
Scatchard Equation ◽  
Novel Method

Molecularly imprinted polymers (MIPs) were prepared by precipitation polymerization with ginsenoside Rg1 as the template molecule. The morphology of MIPs was characterized by scanning electronmicroscope (SEM) and its static adsorption capacity was measured by the Scatchard equation. Scatchard analysis revealed that the homogeneous binding sites were formed in the polymers. The application of MIPs with high affinity toward the template molecule might offer a novel method for the enrichment and determination of active compounds in the traditional herbal medicine.

scholarly journals Compositional Analysis and Aroma Evaluation of Feijoa Essential Oils from New Zealand Grown Cultivars

Molecules ◽  
2019 ◽  
Vol 24 (11) ◽  
pp. 2053 ◽  
Author(s):  
Yaoyao Peng ◽  
Karen Suzanne Bishop ◽  
Siew Young Quek
Keyword(s):  
New Zealand ◽  
Essential Oil ◽  
Essential Oils ◽  
Extraction Method ◽  
Compositional Analysis ◽  
Extraction Methods ◽  
Essential Oil Composition ◽  
Oil Composition ◽  
Active Compounds ◽  
Initial Comparison

Feijoa is an aromatic fruit and the essential oil from feijoa peel could be a valuable by-product in the juicing industry. An initial comparison of the essential oil extraction methods, steam-distillation and hydro-distillation, was conducted. The volatile compounds in the essential oils from four feijoa cultivars were identified and semi-quantified by GC-MS and the aroma active compounds in each essential oil were characterized using SPME-GC-O-MS. Hydro-distillation, with a material to water ratio of 1:4 and an extraction time of 90 min, was the optimized extraction method for feijoa essential oil. The Wiki Tu cultivar produced the highest essential oil yield among the four selected cultivars. A total of 160 compounds were detected, among which 90 compounds were reported for the first time in feijoa essential oils. Terpenes and esters were dominant compounds in feijoa essential oil composition and were also major contributors to feijoa essential oil aroma. Key aroma active compounds in feijoa essential oils were α-terpineol, ethyl benzoate, (Z)-3-hexenyl hexanoate, linalool, (E)-geraniol, 2-undecanone, 3-octanone, α-cubebene, and germacrene D. This is the first report on the optimization of the extraction method and the establishment of the aroma profile of feijoa essential oils, with a comparison of four New Zealand grown cultivars.

Preparation of Henriol C Molecularly Imprinted Polymer Microspheres

2014 ◽  
Vol 664 ◽  
pp. 34-37
Author(s):  
Yong Ming Luo ◽  
Yong Dong Luo ◽  
Fang You Chen ◽  
Xue Lian Zhang ◽  
Xiao Ying Yin
Keyword(s):  
Binding Affinity ◽  
Solid Phase ◽  
Scatchard Analysis ◽  
Template Molecule ◽  
Imprinted Polymer ◽  
Functional Monomers ◽  
Novel Method ◽  
Stereo Selectivity ◽  
High Binding Affinity

Molecular imprinted polymer micrcosheres (MIPMs) were prepared through precipitation polymerization by henriol C using as the template molecule. The morphologies of synthesized MIPMs were characterized by scanning electronmicroscope (SEM). Systematic investigations of the influences of key synthetic conditions, including functional monomers, cross-linkers and porogens, on the morphologies, yields and the recognition properties of the MIPs were conducted. The results indicated that the morphologies of MIPs with DVB as cross-linker was perfect, but their binding affinity is lower than that of MIPs with TRIM or EDMA as cross-linkers. And particle size of MIPs with TRIM as cross-linkers is small but with high binding affinity. Scatchard analysis revealed that the homogeneous binding sites were formed in the polymers. The application of MIPs with high affinity and excellent stereo-selectivity toward henriol C in solid-phase extraction (SPE) column might offer a novel method for the enrichment and determination of sesquiterpenoids in the traditional herbal medicine.

scholarly journals Effect of DNA Extraction Method on the Apparent Microbial Diversity of Soil

2010 ◽  
Vol 76 (10) ◽  
pp. 3378-3382 ◽  
Author(s):  
Özgül İnceoǧlu ◽  
Eelco F. Hoogwout ◽  
Patrick Hill ◽  
Jan Dirk van Elsas
Keyword(s):  
Extraction Method ◽  
Denaturing Gradient Gel Electrophoresis ◽  
Agricultural Soils ◽  
Extraction Methods ◽  
The Novel ◽  
Soil Dna ◽  
Dna Extraction Method ◽  
Gradient Gel Electrophoresis ◽  
Novel Method ◽  
Microbial Groups

ABSTRACT Four extraction methods, including a novel one, were compared for their efficiencies in producing DNA from three contrasting agricultural soils. Molecular analyses (PCR-denaturing gradient gel electrophoresis [DGGE] and clone libraries) focusing on different microbial groups were used as assessment criteria. Per soil, the DNA yields differed between extraction methods. Clear effects of method on apparent richness and community structure were found. Actinobacterial diversity based on soil DNA produced by two divergent methods revealed that a hitherto-undescribed group was obtained by the novel method.

scholarly journals Synthesis and characterization of genistein magnetic molecularly imprinted polymers and their application in soy sauce products

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Ziqi Xie ◽  
Yunjing Luo ◽  
Zhen Na ◽  
Wei Zhang ◽  
Yufei Zong
Keyword(s):  
Electron Microscopy ◽  
Molecularly Imprinted Polymers ◽  
Solid Phase ◽  
Soy Sauce ◽  
Template Molecule ◽  
Molecularly Imprinted ◽  
Imprinted Polymers ◽  
Magnetic Molecularly Imprinted Polymers ◽  
Relative Standard ◽  
Surface Molecular Imprinting Technique

AbstractIn this study, a novel method based on genistein magnetic molecularly imprinted polymers (Gen-MMIPs) was developed utilizing a surface molecular imprinting technique, in which genistein was used as the template molecule and Fe3O4 was used as the carrier. The synthesis of Gen-MMIPs was characterized by using scanning electron microscopy (SEM) and transmission electron microscopy (TEM), which indicated that the diameter of the Gen-MMIPs was approximately 500 nm. Via analysis with a vibrating sample magnetometer (VSM), the saturation magnetization of Gen-MMIPs was determined to be 24.79 emu g−1. Fourier transform infrared (FT-IR) spectroscopy showed that polymer groups were on the surface of the magnetic carrier. Adsorption experiments suggested that the genistein adsorption capability of Gen-MMIPs was 5.81 mg g−1, and adsorption equilibrium was achieved within 20 min. Gen-MMIPs as dispersive solid-phase extraction (dSPE) adsorbents combined with HPLC were used to selectively separate genistein in soy sauce samples, and the recoveries ranged from 85.7 to 88.5% with relative standard deviations (RSDs) less than 5%, which proved that this method can be used for the detection of genistein residues in real samples.

scholarly journals Synthesis and Characterization of Genistein Magnetic Molecularly Imprinted Polymers and Its Application in Soy Sauce Products

2021 ◽  
Author(s):  
Ziqi Xie ◽  
Yunjing Luo ◽  
Zhen Na ◽  
Wei Zhang ◽  
Yunfei Zong
Keyword(s):  
Electron Microscopy ◽  
Molecularly Imprinted Polymers ◽  
Solid Phase ◽  
Soy Sauce ◽  
Template Molecule ◽  
Molecularly Imprinted ◽  
Imprinted Polymers ◽  
Magnetic Molecularly Imprinted Polymers ◽  
Relative Standard ◽  
Surface Molecular Imprinting Technique

Abstract In this study, a novel method based on genistein magnetic molecularly imprinted polymers (Gen-MMIPs) was developed by surface molecular imprinting technique, in which genistein was used as the template molecule and Fe3O4 was used as the carrier. The synthesis of Gen-MMIPs were characterized by using of scanning electron microscopy (SEM) and transmission electron microscopy (TEM), which indicated the diameters of Gen-MMIPs were about 500 nm. Through the technique of vibrating sample magnetometer (VSM), the saturation magnetization of Gen-MMIPs were detected as 24.79 emu/g. Fourier transform infrared (FR-IR) spectroscopy showed that polymer groups were on the surface of the magnetic carrier. Adsorption experiment suggested the adsorption capability of Gen-MMIPs to genistein were 1.55 mg/g, and 2 the adsorption equilibrium was achieved within 20 min. Gen-MMIPs as dispersive solid-phase extraction adsorbent combined with HPLC was used to selectively separate genistein in soy sauce samples, the recoveries were ranged from 85.7% to 88.5% with the relative standard deviations (RSD) less than 5%, which proved this method can be used for the detection of genistein residues in real samples.

scholarly journals Comparison and Identification of the Aroma-Active Compounds in the Root of Angelica dahurica

Molecules ◽  
2019 ◽  
Vol 24 (23) ◽  
pp. 4352
Author(s):  
Die Hu ◽  
Junrui Guo ◽  
Ting Li ◽  
Mu Zhao ◽  
Tingting Zou ◽  
...  
Keyword(s):  
Solid Phase Microextraction ◽  
Solid Phase ◽  
Principal Component ◽  
Extraction Methods ◽  
Aroma Compounds ◽  
Dynamic Headspace ◽  
Active Compounds ◽  
Three Samples ◽  
Dynamic Headspace Sampling

Solid-phase microextraction (SPME), purge and trap (P&T), stir bar sportive extraction (SBSE), and dynamic headspace sampling (DHS) were applied to extract, separate and analyze the volatile compounds in the roots of Hangbaizhi, Qibaizhi, and Bobaizhi and the GC-O-MS/MS (AEDA) was utilized for the quantification of key aroma compounds. Totals of 52, 54, and 43 aroma-active compounds extracted from the three samples by the four extraction methods were identified. Among these methods, the SPME effectively extracted the aroma compounds from the A. dahurica. Thus, using the SPME methods for quantitative analysis based on external standards and subsequent dilution analyses, totals of 20, 21, and 17 aroma compounds were detected in the three samples by the sniffing test, and sensory evaluations indicated that the aromas of A. dahurica included herb, spice, and woody. Finally, principal component analysis (PCA) showed that the three kinds A. dahurica formed three separate groups, and partial least squares discriminant analysis (PLS-DA) showed that caryophyllene, (−)-β-elemene, nonanal, and β-pinene played an important role in the classification of A. dahurica.

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