The Expression of HIF-L a and VEGF in Endochondral Ossification Processes

2013 ◽  
Vol 773 ◽  
pp. 342-346
Author(s):  
Wang Song ◽  
Jin Xing
Keyword(s):  
Protein Expression ◽  
Mrna Expression ◽  
Endochondral Ossification ◽  
Bone Development ◽  
Femoral Bone ◽  
Rt Pcr ◽  
Vegf Expression ◽  
Chain Reaction ◽  
Cartilage Cells ◽  
Polymerase Chain

Objective To investigate the expression of HIF-la in entochondrostosis,and to study its role in the process of vascular intrusion.Methods The morphology change in rats femoral bone development were observed using HE staining method.The protein expression Of HIF-Ia and VEGF in femoral bone was detected by immunohistochemistry,and the mRNA expression of HIF-Ia and VEGF was detected by reverse transcription-polymerase chain reaction (RT-PCR) method.Results: Many cartilage cells were seen in femoral bone at the 1st day after the birth;many vessels began to invade at the 7th dayfat the 13th day,the cartilage matrix began to calcification,vessels and cartilage cells were reduced.The protein expression of HIF-la and VEGF was increased at the 7th daywhile decreased at the 13th day.The mRNA expression of HIF-la and VEGF went up to the maximum at 7th day the time,and then decreased.Conclusion During femoral bone development,hypoxia may induce HIF-I expression,then activates its downstream gene VEGF expression,which would induce the intrusion of exogenous vascular.Promote endochondral osteogenesis.

Gene expressions of TS and MDR-1 are predictive factors for chemosensitivity and survival in esophageal cancer with fluoropyrimidine-based chemotherapy

2007 ◽  
Vol 25 (18_suppl) ◽  
pp. 14120-14120
Author(s):  
K. Uchida ◽  
K. Hayashi ◽  
K. Kudo ◽  
H. Kuramochi ◽  
M. Yamamoto
Keyword(s):  
Esophageal Cancer ◽  
Mrna Expression ◽  
Overall Response Rate ◽  
Response Rate ◽  
Rt Pcr ◽  
Chain Reaction ◽  
Gene Expressions ◽  
Polymerase Chain ◽  
Relative Mrna Expression ◽  
Mdr 1

14120 Background: Fluoropyrimidines are widely used in chemotherapy regimens for esophageal cancer. To test the hypotheses of whether the relative mRNA expression of the enzyme TS, DPD and MDR-1 were associated with response to in esophageal cancer. We investigated the associations reported between intratumoral TS, DPD and MDR-1 gene expressions and the response with 5-FU based chemotherapy for patients with esophageal cancer. Methods: Twenty six pts with esophageal cancer were treated with 5-FU based chemotherapy (14 pts were treated with chemotherapy only, and 12 pts were chemoradiotherapy). mRNA was isolated from frozen tumor specimens, and relative expression levels of each gene/β-actin were measured using a quantitative reverse transcription polymerase chain reaction (RT-PCR) (Taqman®) system. Results: The overall response rate was 50.0%. Each mRNA expression was detectable in 26 patients. TS expressions were significantly lower in the responding tumors compared to the non-responders respectively (P=0.009). There were no significant associations between MDR-1 expression and the response. By setting up a cut-off level for TS and MDR-1 of Median, combining the two gene expression two-dimensionally revealed a relationship with the response (P=0.037). Additionally, MDR-1 expressions were statistically significant predictors of prolonged survival (P=0.0084). Conclusions: These results suggest that intratumoral TS and MDR-1 expressions are prognostic factors for survival after 5-FU based chemotherapy in esophageal cancer. No significant financial relationships to disclose.

5-HT2B receptors are expressed on astrocytes from brain and in culture and are a chronic target for all five conventional ‘serotonin-specific reuptake inhibitors’

2010 ◽  
Vol 6 (2) ◽  
pp. 113-125 ◽  
Author(s):  
Shiquen Zhang ◽  
Baoman Li ◽  
Ditte Lovatt ◽  
Junnan Xu ◽  
Dan Song ◽  
...  
Keyword(s):  
Protein Expression ◽  
Cell Sorting ◽  
Cultured Cells ◽  
Primary Cultures ◽  
Rt Pcr ◽  
Chain Reaction ◽  
Calcium Dependent ◽  
Mrna And Protein Expression ◽  
Polymerase Chain ◽  
Well Differentiated

In well-differentiated primary cultures of mouse astrocytes, which express no serotonin transporter (SERT), the ‘serotonin-specific reuptake inhibitor’ (SSRI) fluoxetine leads acutely to 5-HT2B receptor-mediated, transactivation-dependent phosphorylation of extracellular regulated kinases 1/2 (ERK1/2) with an EC50 of ~5 μM, and chronically to ERK1/2 phosphorylation-dependent upregulation of mRNA and protein expression of calcium-dependent phospholipase A2 (cPLA2) with ten-fold higher affinity. This affinity is high enough that fluoxetine given therapeutically may activate astrocytic 5-HT2B receptors (Li et al., 2008, 2009). We now confirm the expression of 5-HT2B receptors in astrocytes freshly dissociated from mouse brain and isolated by fluorescence-activated cell sorting (FACS) and investigate in cultured cells if the effects of fluoxetine are shared by all five conventional SSRIs with sufficiently high affinity to be relevant for mechanism(s) of action of SSRIs. Phosphorylated and total ERK1/2 and mRNA and protein expression of cPLA2a were determined by Western blot and reverse transcription polymerase chain reaction (RT-PCR). Paroxetine, which differs widely from fluoxetine in affinity for SERT and for another 5-HT2 receptor, the 5-HT2C receptor, acted acutely and chronically like fluoxetine. One micromolar of paroxetine, fluvoxamine or sertraline increased cPLA2a expression during chronic treatment; citalopram had a similar effect at 0.1–0.5 μM; these are therapeutically relevant concentrations.

The Effects of Different Hydroxyapatite/TiO2 Composite Coatings on Protein Expression of Osteoblast

2009 ◽  
Vol 610-613 ◽  
pp. 1104-1108 ◽  
Author(s):  
Jian Ye Han ◽  
Zhen Tao Yu ◽  
Lian Zhou
Keyword(s):  
Protein Expression ◽  
Type I Collagen ◽  
Composite Coatings ◽  
Type I ◽  
X Ray Diffraction ◽  
Rt Pcr ◽  
Chain Reaction ◽  
Compound Formation ◽  
X Ray ◽  
Polymerase Chain

Hydroxyapatite/TiO2 composite material was coated onto Ti25Nb3Mo2Sn3Zr (TLM) alloy substrate. To study the effects of hydroxyapatite/TiO2 composite coatings on bone-related protein expression, the osteoblast were cultured with composite coatings for different times. The phase transformation and compound formation of the HA/TiO2 coatings were investigated using XRD (X-ray diffraction). The mRNA expression of Type I collagen, alkaline phosphatase (ALP) and osteocalcin were studied by RT-PCR (reverse transcriptional polymerase chain reaction). The titania delayed the crystallization of HA. The mRNA expressions of Type I collagen are decreased as the increasing of TiO2 percentage. The mRNA expressions of osteocalcin are approached. The ALP expression on H4 coating (HA/TiO2 mol ration is 5) after the osteoblast cultured with composite coating for 6 days is the highest. The increasing of TiO2 amount decreases the bioactivity of the composite coatings.

Cykotine mRNA expression in mouse retina after laser injury by reverse transcriptase-polymerase chain reaction (RT-PCR)

1996 ◽  
Author(s):  
Steven T. Schuschereba ◽  
Phillip D. Bowman ◽  
Veronica Ujimore ◽  
Stephen W. Hoxie ◽  
Jose M. Pizarro ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Reverse Transcriptase ◽  
Mrna Expression ◽  
Mouse Retina ◽  
Rt Pcr ◽  
Chain Reaction ◽  
Laser Injury ◽  
Polymerase Chain

D3 dopamine receptor mRNA expression in lymphocytes: a peripheral marker for schizophrenia?

2003 ◽  
Vol 15 (2) ◽  
pp. 91-93 ◽  
Author(s):  
J. van der Weide ◽  
L. S. W. Steijns ◽  
M. A. M. van der Geld ◽  
P. A. de Groot
Keyword(s):  
Mrna Expression ◽  
Dopamine Receptor ◽  
Diagnostic Value ◽  
D3 Receptor ◽  
Rt Pcr ◽  
Chain Reaction ◽  
Receptor Mrna ◽  
Schizophrenic Patients ◽  
Polymerase Chain ◽  
Antipsychotic Drug Treatment

Background:Identification of schizophrenia, a common neuropsychiatric disorder, is based on clinical examination. An easily measurable peripheral marker, which may enable a more rapid and more accurate diagnosis, is not available. A possible candidate is the D3 dopamine receptor on lymphocytes.Objective:The D3 receptor is investigated for its clinical significance as a marker for diagnosing schizophrenia.Methods:From eight schizophrenic patients and eight controls lymphocyte RNA was isolated. A semiquantitative reverse transcription–polymerase chain reaction (RT-PCR) was carried out and the intensities of the specific D3 dopamine receptor bands of patients and controls were compared.Results:No difference could be seen between the intensities of the bands from patients and controls.Conclusion:An aberrant D3 dopamine receptor mRNA expression in lymphocytes of schizophrenics could not be demonstrated. This might be caused by down-regulation of D3 receptor production by antipsychotic drug treatment. At present, the D3 receptor seems to have no diagnostic value in schizophrenia.

scholarly journals Inactivation of parkin by promoter methylation correlated with lymph node metastasis and genomic instability in nasopharyngeal carcinoma

Tumor Biology ◽  
2017 ◽  
Vol 39 (3) ◽  
pp. 101042831769502 ◽  
Author(s):  
Haifeng Ni ◽  
Zhen Zhou ◽  
Bo Jiang ◽  
Xiaoyang Yuan ◽  
Xiaolin Cao ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Nasopharyngeal Carcinoma ◽  
Protein Expression ◽  
Mrna Expression ◽  
Genomic Instability ◽  
Promoter Methylation ◽  
Chain Reaction ◽  
Node Metastasis ◽  
Mrna And Protein Expression ◽  
Polymerase Chain

This study aimed to investigate the inactivation of the parkin gene by promoter methylation and its relationship with genome instability in nasopharyngeal carcinoma. Parkin was considered as a tumor suppressor gene in various types of cancers. However, its role in nasopharyngeal carcinoma is unexplored. Genomic instabilities were detected in nasopharyngeal carcinoma tissues by the random amplified polymorphic DNA. The methylation-specific polymerase chain reaction, semi-quantitative reverse transcription polymerase chain reaction, and immunohistochemical analysis were used to detect methylation and mRNA and protein expression of parkin in 54 cases of nasopharyngeal carcinoma tissues and 16 cases of normal nasopharyngeal epithelia tissues, and in 5 nasopharyngeal carcinoma cell lines (CNE1, CNE2, TWO3, C666, and HONE1) and 1 normal nasopharyngeal epithelia cell line (NP69). mRNA expression of parkin in CNE1 and CNE2 was analyzed before and after methyltransferase inhibitor 5-aza-2-deoxycytidine treatment. The relationship between promoter methylation and mRNA expression, demethylation and mRNA expression, and mRNA and protein expression of the gene and clinical factors and genomic instabilities were analyzed. The mRNA and protein expression levels were significantly reduced in 54 cases of human nasopharyngeal carcinoma compared with 16 cases of normal nasopharyngeal epithelia. Parkin-methylated cases showed significantly lower mRNA and protein expression levels compared with unmethylated cases. After 5-aza-2-deoxycytidine treatment, parkin mRNA expression was restored in CNE1 and CNE2; 92.59% (50/54) of nasopharyngeal carcinoma demonstrated genomic instability. Parkin is frequently inactivated by promoter methylation, and its mRNA and protein expression correlate with lymph node metastasis and genomic instability. Parkin deficiency probably promotes tumorigenesis in nasopharyngeal carcinoma.

1504: Molecular Diagnosis and Staging of Prostate Cancer Using Clusterin in Real Time Reverse Transcription Polymerase Chain Reaction (RT-PCR)

2006 ◽  
Vol 175 (4S) ◽  
pp. 485-486
Author(s):  
Sabarinath B. Nair ◽  
Christodoulos Pipinikas ◽  
Roger Kirby ◽  
Nick Carter ◽  
Christiane Fenske
Keyword(s):  
Prostate Cancer ◽  
Polymerase Chain Reaction ◽  
Real Time ◽  
Molecular Diagnosis ◽  
Reverse Transcription ◽  
Rt Pcr ◽  
Chain Reaction ◽  
Polymerase Chain
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