Study on Nutrient Removal Performance and Biological Community Structure in Hybrid Biofilm Reactor

2013 ◽  
Vol 777 ◽  
pp. 213-220 ◽  
Author(s):  
Lu Zhou ◽  
Ge Li ◽  
Hang Sik Shin ◽  
Li Li Jiang ◽  
Guo Feng Fang ◽  
...  
Keyword(s):  
Community Structure ◽  
Nutrient Removal ◽  
Diversity Index ◽  
Batch Reactor ◽  
Similarity Coefficient ◽  
Biofilm Reactor ◽  
Nitrifying Bacteria ◽  
Nitrogen And Phosphorus ◽  
Biological Community ◽  
Dominant Bacteria

To investigate nutrient removal and biological community structure in wastewater treatment system under different ratio of C/N/P, hybrid biofilm reactor (HBR) and sequencing batch reactor (SBR) in lab scale were set up. Results showed that both HBR and SBR achieved the best removal efficiency of nitrogen and phosphorus when C/N/P=100/6.25/1. With the rise of C/N/P from 50/6.25/1 to 100/6.25/1, the removal efficiencies of NH3-N were greater than 93% in three phases of both reactors; the efficiencies of TN were increased from 65% to 79% in SBR while these values at HBR were from 73% to 86%; the efficiencies of TP were increased from 42% to 93% in SBR while these values at HBR were from 32% to 93%. Microbial community structure response to different C/N/P was determined by PCR-DGGE technology. Similarity coefficient was lowest and denitrifying phosphate accumulating bacteria became dominant bacteria in SBR when C/N/P was 100/6.25/1. There were many functional bacteria in HBR, such as bacteria for removing nutrients (Uncultured BacteroidetesFJ828150), bacteria for removing organic (Uncultured Saprospiraceae) bacteria for removing nitrogen (Ferribacterium sp.), and nitrifying bacteria (Uncultured Nitrospira sp.) which became dominant bacteria. Because of microbial diversity index of biofilm was up to 3.3, similarity coefficient was as low as 43.4%.

scholarly journals Phytoplankton community structure in Menjer Lake, Central Java

2021 ◽  
Vol 869 (1) ◽  
pp. 012037
Author(s):  
F Sulawesty ◽  
G P Yoga ◽  
L Subehi ◽  
R Rosidah
Keyword(s):  
Community Structure ◽  
Phytoplankton Community ◽  
Diversity Index ◽  
Nutrient Content ◽  
Cell Counting ◽  
Phytoplankton Community Structure ◽  
Nitrogen And Phosphorus ◽  
Central Java ◽  
Phytoplankton Communities ◽  
Ecological Pressure

Abstract The occurrence of land changes around Lake Menjer, Central Java Province will affect the condition of water quality subsequently will affect biota, including phytoplankton. The purposes of study was to analyze the composition and abundance of phytoplankton in Menjer Lake regarding to nutrient content i.e. nitrogen and phosphorus. Observations on the phytoplankton community were conducted in July and October 2019 at six locations in Menjer Lake. Water samples were taken at the water surface as much as 10 L filtered using plankton net. Identification was carried out under the inverted microscope Diaphot 300. The abundance was calculated using the Sedgwick Rafter cell counting (SRCC) method. Analysis of the phytoplankton community structure was derived by calculating the Diversity Index, Evenness Index, and Simpson Dominance Index. The nutrient content in Menjer Lake influences the structure of phytoplankton communities quantitatively and qualitatively. Observation showed that the diversity of phytoplankton was low and there was one species dominanted, the result explained that the phytoplankton community in Menjer Lake is unstable and there is ecological pressure on the community. It can be concluded that the improvement of environmental conditions around Menjer Lake is the basis for the sustainable management of Menjer Lake.

Biological nitrogen and phosphorus removal in an anaerobic/anoxic sequencing batch reactor with separated biofilm nitrification

1994 ◽  
Vol 30 (6) ◽  
pp. 303-313 ◽  
Author(s):  
G. Bortone ◽  
F. Malaspina ◽  
L. Stante ◽  
A. Tilche
Keyword(s):  
Organic Carbon ◽  
Phosphorus Removal ◽  
Sequencing Batch Reactor ◽  
Batch Reactor ◽  
P Uptake ◽  
Biofilm Reactor ◽  
Nitrogen And Phosphorus ◽  
Piggery Wastewater ◽  
Organic Carbon Oxidation ◽  
Biological Nitrogen

An Anaerobic/Anoxic Sequencing Batch Reactor (A/A SBR) with separated batch biofilm nitrification was tested for nutrient removal against a five step Anaerobic-anoxic/Oxic SBR (A/O SBR). Piggery wastewater, particularly challenging for its low COD/N ratio, was used as feed. After feeding, the A/A SBR ran under anaerobic conditions for organic carbon sequestering and phosphorus removal. A settling phase was allowed to separate an ammonia-rich supernatant to be nitrified in a external biofilm reactor. The nitrified effluent returned to the A/A SBR where nitrates were removed, being used as final electron acceptors for luxury P-uptake and organic carbon oxidation. A/A SBR showed very good N and P removal capacities with excellent sludge settling properties. On the other hand, organic carbon removal efficiency with nitrate was lower than with oxygen. Batch biofilm nitrification was very effective, with very high nitrification rates. Presence of poly-P bacteria in the A/A SBR sludge was assessed through microscopic observation and from the high cellular poly-phosphate content.

Feasibility Study on Biological Nutrient Removal in Sequencing Batch Reactor Treating Municipal Wastewater

2020 ◽  
Vol 17 (2) ◽  
pp. 946-949
Author(s):  
Samaneh Alijantabar Aghouzi ◽  
Thomas S. Y. Choong ◽  
M. I. Aida Isma
Keyword(s):  
Nutrient Removal ◽  
Sequencing Batch Reactor ◽  
Municipal Wastewater ◽  
Batch Reactor ◽  
Ammonia Nitrogen ◽  
Biological Nutrient Removal ◽  
Nitrogen And Phosphorus ◽  
Bacterial Consortia ◽  
Aeration Time ◽  
Working Volume

This study elucidates the performance of sequencing batch reactor for nutrient removal from municipal wastewater. The removal of COD, ammonia nitrogen and phosphorus were investigated. The SBR with a working volume of 5 L was operated for 6 hours, with 5 min fill, 30 min settle and 5 min effluent withdrawal. The remaining time in each cycle was 90 min anaerobic phase, 130 min anoxic phase and 110 min aerobic phase. The experiment was repeated with a longer aeration time of 180 min resulting to prolong the duration cycle. In the aerobic phase, dissolved oxygen was kept in the range of more than 2 mg/L. During batch operation, the system attained stability and had a removal efficiency for ammonia nitrogen, COD and phosphorus of 51.36%, 83.33% and 99.53%, respectively. Extending the aeration period improved ammonia nitrogen removal to 54.27%. It should be noted that the stability of the granular biomass agglomerates highly depending on the bacterial consortia. The particle size of sludge reduced from 60.26 μm to 39.00 μm in 60 days. It was observed that degranulation process and biomass loss was unavoidable.

Enhanced Nutrient Removal in Porous Biomass Carrier Sequencing Batch Reactor (PBCSBR)

1991 ◽  
Vol 23 (4-6) ◽  
pp. 719-728 ◽  
Author(s):  
Hang-Sik Shin ◽  
Hung-Suck Park
Keyword(s):  
Nutrient Removal ◽  
Sequencing Batch Reactor ◽  
Batch Reactor ◽  
Operation Mode ◽  
Biomass Concentration ◽  
Phosphorus Release ◽  
Organic Substrate ◽  
Nitrogen And Phosphorus ◽  
Behavioural Patterns ◽  
Relationship Of

An investigation was made theoretically and experimentally on the porous biomass carrier sequencing batch reactor (PBCSBR) for enhanced nutrient removal. Biomass hold-up increased with incoming organic substrate concentration and held in attached and entrapped conditions. The behavioural patterns of organic carbon, nitrogen and phosphorus in PBCSBR were similar to the control SBR reactor. Nitrogen transformation and/or removal was simultaneous and stoichiometric and could be quantified by the stoichiomelric relationship of nitrification/denitrification based on the consumed alkalinity. Phosphorus removal increased with biomass concentration and phosphorus release. Higher biomass and favorable operation mode in PBCSBR were conducive to enhanced nutrient removal.

Extracellular polymeric substances in relation to nutrient removal from a sequencing batch biofilm reactor

2001 ◽  
Vol 43 (6) ◽  
pp. 185-192 ◽  
Author(s):  
E. Choi ◽  
Z. Yun ◽  
Y. Park ◽  
H. Lee ◽  
H. Jeong ◽  
...  
Keyword(s):  
Nitrogen Removal ◽  
Nutrient Removal ◽  
Phosphorus Removal ◽  
Biofilm Reactor ◽  
Bulk Liquid ◽  
Growth Media ◽  
Nitrogen And Phosphorus ◽  
Nitrogen And Phosphorus Removal ◽  
Biofilm Growth ◽  
Biological Nitrogen

Experimental investigations were performed to determine the possibility of simultaneous biological nitrogen and phosphorus removal during various biofilm processes in conjunction with biofilm characterisation, especially extracellular polymeric substance (EPS). Since biological nitrogen removal requires an alternating exposure of anaerobic-anoxic-oxic conditions in the bulk liquid that surrounds the biofilm growth media, a sequencing batch reactor (SBR)-type operation was used. Various materials including expanded clay, polystyrene, polyurethane, and acrylic materials were used as the biofilm growth support medium. Simultaneous nitrogen and phosphorus removal was possible with SBR, but it was postulated that nutrient removal efficiencies varied with film thickness. Thinner biofilm promoted nitrification and phosphorus removal, but thicker biofilm enhanced denitrification and reduced phosphorus removal. EPS contents were similar regardless of support media types or biofilm configuration, but EPS contents gradually increased as the film growth continued after backwashing. EPS contents were increased with increased nitrogen removal, but it was difficult to define its relation with phosphorus removal. In addition, suspended solids removal was correlated well with the EPS content in the biofilms.

Evaluation of sequencing batch reactor (SBR) and sequencing batch biofilm reactor (SBBR) for biological nutrient removal from simulated wastewater containing glucose as carbon source

2003 ◽  
Vol 48 (3) ◽  
pp. 73-79 ◽  
Author(s):  
B. Manoj Kumar ◽  
S. Chaudhari
Keyword(s):  
Carbon Source ◽  
Phosphorus Removal ◽  
Batch Reactor ◽  
Biofilm Reactor ◽  
Synthetic Wastewater ◽  
Biological Nutrient Removal ◽  
Cow Dung ◽  
Nitrogen And Phosphorus ◽  
Seed Culture ◽  
Suspended Biomass

In general, conventional activated sludge (ASP) or enhanced biological phosphorus removing (EBPR) sludge has been used as seed culture for developing EBPR sludge and the time reported for development varies from months to year. In the present study cow-dung has been used as seed culture and EBPR sludge was developed within 36 days. The developed EBPR sludge has been used to evaluate the performance of sequential batch reactor (SBR) and sequential batch biofilm reactors (SBBR) for simultaneous nitrogen and phosphorus removal from synthetic wastewater containing glucose as carbon source. Three reactors were operated, SBR-1 containing only suspended biomass, SBBR-2 and SBBR-3 containing 5% and 10% polyurethane foam (PUF) media respectively along with suspended biomass. In all the reactors phosphorus removal was nearly the same and was more than 80%. In all the three reactors greater than 90% nitrification was achieved. Nitrogen removal in SBR-1 was 48% and in SBBR-2 and SBBR-3 it was more than 62%. On line monitoring of oxidation-reduction potential (ORP), pH and phosphorus during a cycle indicated that ORP and pH can be useful for real time control and optimization of the process.

scholarly journals Characteristics of the community-structure of A2O processes under different temperature conditions in plateau areas

2020 ◽  
Author(s):  
Yongchen Zong ◽  
Yuanwei Li ◽  
Decai Huang ◽  
Kaiyue Hao ◽  
Guanghua Lu
Keyword(s):  
Community Structure ◽  
Operating Conditions ◽  
Community Diversity ◽  
Nitrifying Bacteria ◽  
Composition Analysis ◽  
Temperature Sensitive ◽  
Colony Structure ◽  
The Common ◽  
Dominant Bacteria ◽  
Study Laboratory

Abstract Background: In this study, laboratory-scale A2O wastewater treatment was performed in Linzhi at an altitude of 3000 meters. Water temperatures were maintained at four operating conditions (25°C, 20°C, 15°C, and 10°C). Sludge in the aerobic tank was assessed by 16SrRNA sequencing and composition analysis. The Phylum, Class, Order, Family, Genus, and Species of the sludge were also confirmed.Results: The bacteria within the sludge showed significant differences at varying temperatures (P<0.05). A significant correlation between the bacteria numbers in anaerobic tanks and anoxic tanks also occurred. Indicators of community richness, community uniformity, community diversity and other areas showed differences. Significant differences in bacteria abundance were also observed and differed to those of previously reported superior community categories and proportions. T-tests were used to identify temperature-sensitive community at each level. Correlation analysis of environmental factors and colony structure further confirmed the association between temperature and colony structure (P<0.05). The removal rates of TP, TN, NH3-N, and COD were all affected by the sample community structure. The variety of colony structure include nitrifying bacteria, denitrifying bacteria, phosphorus accumulating bacteria and other bacterial differed, but their proportions were relatively low. Heatmaps were used to identify species sensitive to temperature, TP, TN, NH3-N, COD at the species level.Conclusions: Amongst the common dominant bacteria at the species level, Bacteroidetes_bacterium_OLB10 was significantly related to temperature. Other common dominant bacteria that were related to temperature metagenome_g__Gordonia, unclassified_f__Burkholderiaceae, uncultured_bacterium_g__norank_f__Saprospiraceae.

Nutrient removal from low strength domestic wastewater in sequencing batch biofilm reactor

2001 ◽  
Vol 44 (1) ◽  
pp. 181-186 ◽  
Author(s):  
U. Altinbas
Keyword(s):  
Nutrient Removal ◽  
Phosphorus Removal ◽  
Domestic Wastewater ◽  
Packed Bed ◽  
Biofilm Reactor ◽  
Nitrogen And Phosphorus ◽  
Nitrogen And Phosphorus Removal ◽  
Packed Bed Column ◽  
Sequencing Batch Biofilm Reactor ◽  
Removal Efficiencies

Nutrient removal was investigated in a packed bed column, which was operated by changing of aerated and non-aerated periods. Good removal efficiencies for nitrogen and phosphorus were obtained at long aeration period. Highest nitrification efficiency was observed in run3 because the aeration period was enough to allow nitrification. NO3 concentration was not significantly changed during the cycle, because of simultaneous denitrification during aerobic stage. Nitrogen and phosphorus removal efficiencies reached to 71 and 74% in run3 respectively. Effluent concentrations of TKN, Tot.P, NH4 and NO3 were found as 3.8, 3, 1 and 2.5 mg/l respectively.

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