Primary Study on Optimization of the Processing Technology in Aspergillus oryzae Seed Koji by Homogeneous Design

2013 ◽  
Vol 798-799 ◽  
pp. 1071-1074
Author(s):  
Ying Ying Ma ◽  
Zhong Kai Zhang ◽  
Xun Hai Pan ◽  
Jian Gang Yang
Keyword(s):  
Aspergillus Oryzae ◽  
Incubation Time ◽  
Culture Conditions ◽  
Wood Ash ◽  
Processing Technology ◽  
Primary Study ◽  
Culture Temperature ◽  
Culture Time ◽  
Spore Number

The homogeneous was used to optimize the processing technology of Aspergillus oryzae seed koji. The results show that the culture time is the most significant factor and the sequence of the residual factors are the square of culture time, the interaction between temperature and the time, the interaction of time and wood ash addition, the square of wood ash addition and the inoculation quantity. The results reveal that the optimized culture conditions are proved to be that the incubation time is 9 days, the inoculation quantity is 0.1×107/g, the dosage of wood ash is 1.267% and the culture temperature is 40°C. Under this condition, the prospective spore number of seed koji has reached to 1.89×109 /g.

Optimization of Process Conditions of Aspergillus oryzae Koji by Response Surface Methodology

2013 ◽  
Vol 798-799 ◽  
pp. 1099-1104
Author(s):  
Ying Ying Ma ◽  
Zhong Kai Zhao ◽  
Qing Bin Liu ◽  
Jian Gang Yang
Keyword(s):  
Response Surface ◽  
Aspergillus Oryzae ◽  
Process Condition ◽  
Process Conditions ◽  
Single Factor ◽  
Optimum Conditions ◽  
Culture Temperature ◽  
Design Expert ◽  
Culture Time ◽  
Glucoamylase Activity

Effects of inoculation quantity, culture temperature and time on glucoamylase activity and the optimum conditions of koji-making of Aspergillus oryzae was studied by using single factor and response surface experiment. The results show that 3 factors can significantly impact on glucoamylase activity of koji. The best process condition of koji-making obtained by using the "Design-Expert" software is: culture temperature 38.70 °C, inoculation quantity 0.85 , and culture time 50.47 h.

Study on the Microbial Hydrolysis of Maize Straw and the Water Reducing Properties of the Hydrolysate

2011 ◽  
Vol 306-307 ◽  
pp. 87-92
Author(s):  
Yuan Xiu Wang ◽  
Gui Xiang Zhang ◽  
Xiao Yan Song ◽  
Na Xin Sun ◽  
Yu Mei Li ◽  
...  
Keyword(s):  
Culture Conditions ◽  
Partial Substitution ◽  
Optimal Conditions ◽  
Culture Temperature ◽  
Culture Time ◽  
Microbial Hydrolysis ◽  
Maize Straw ◽  
Hydrolysis Of ◽  
Fermentation Stage ◽  
Reducing Properties

A two-step fermentation procedure for maize straw hydrolysis by Phanerochaete chrysosporium, Trichoderma and Aspergillus nigerwere investigated in this study. 2 mL of P. chrysosporium (107spores/mL) wasadded to 50 mL medium for the first fermentation stage. The optimal culture conditions were 28°C of culture temperature, 4.0 of pH, 8 days of culture time, 2 mL of Trichoderma suspensions (107spores/mL) and 1 mL of A. niger suspensions (107spores/mL) of the inoculums size on the second fermentation stage. Under the optimal conditions, the crude degradation rate achieved 48.2%. Via sulfonation, oxidate-lignosulfonate alkali lignin (OLAL) was achieved from maize straw hydrolysate(MSH).OLAL and MSH can be partial substitution for common water reducers and OLAL was more efficient compared to that of MSH.

scholarly journals The optimization of fermentation conditions for producing cellulase of Bacillus amyloliquefaciens and its application to goose feed

2017 ◽  
Vol 4 (10) ◽  
pp. 171012 ◽  
Author(s):  
Miao Ye ◽  
Linghong Sun ◽  
Ru Yang ◽  
Zaigui Wang ◽  
KeZong Qi
Keyword(s):  
Incubation Time ◽  
Bacillus Amyloliquefaciens ◽  
Egg Production ◽  
Fertilization Rate ◽  
Culture Conditions ◽  
Liquid Volume ◽  
Hatching Rate ◽  
Culture Temperature ◽  
Egg Weight ◽  
Initial Ph

The proper culture conditions for producing cellulase of Bacillus amyloliquefaciens S1, isolated from the cecum of goose was optimized by single-factor experiment combined with orthogonal test. The properties of the cellulase were investigated by DNS method. The appropriate doses of B. amyloliquefaciens S1 were obtained by adding them to goose feed. It indicated that the suitable culture conditions of producing cellulase were the culture temperature of 37°C, the initial pH of 7.0, the incubation time of 72 h and the loaded liquid volume of 75 ml per 250 ml. The effects of each factor on producing cellulase by B. amyloliquefaciens S1 were as follows: initial pH > incubation time = culture temperature > loaded liquid volume. The optimum reaction temperature and pH were 50°C and 7.0, respectively. This enzyme is a kind of neutral cellulase that possesses resistance to heat and acidity. It showed high activity to absorbent cotton, soya bean meal and filter paper. By adding different doses of B. amyloliquefaciens S1 to the goose feed, it was found that the egg production, average egg weight, fertilization rate and the hatching rate were promoted both in experiment 1 (1.5 g kg −1 ) and experiment 2 (3 g kg −1 ). Also the difference of egg production, fertilization rate and hatching rate between experiment 1 and control group was obvious ( p  < 0.05), and the average egg weight was significantly increased in experiment 2 ( p  < 0.05).

Fructose 2,6-bisphosphate biosynthesis and regulation of carbohydrate metabolism in Aspergillus oryzae

1998 ◽  
Vol 44 (1) ◽  
pp. 6-11
Author(s):  
Gandhi Rádis-Baptista ◽  
David N. Urquizo Valdivia ◽  
José Abrahão-Neto
Keyword(s):  
Cyclic Amp ◽  
Carbohydrate Metabolism ◽  
Aspergillus Oryzae ◽  
Culture Conditions ◽  
Dependent Protein Kinase ◽  
Fructose 1,6 Bisphosphatase ◽  
Dependent Protein ◽  
Regulation Of Carbohydrate Metabolism

The biosynthesis and role of fructose 2,6-bisphosphate (Fru-2,6-P2) in carbohydrate metabolism during induction of an amylolytic system in Aspergillus oryzae was studied. Fluctuations in Fru-2,6-P2 were not dependent on the external glucose concentration during induction, whereas the level of Fru-2,6-P2 increased significantly when the oxygen concentration was diminished. Phosphofructokinase II (PFK II) of A. oryzae was sensitive to phosphorylation in vitro by the catalytic subunit of cyclic AMP dependent protein kinase, which increased the Vmax (twofold), although the Km (0.7 mM) remained unchanged. Phosphofructokinase I was neither activated by micromolar Fru-2,6-P2 nor inhibited by high ATP concentrations. The activity of fructose-1,6-bisphosphatase (FBPase) was subject to strong inhibition by Fru-2,6-P2. Addition of glucose to cultures under gluconeogenic conditions caused a decrease of approximately 40% in the FBPase activity within 4 min. These results indicate that the effect of Fru-2,6-P2 in A. oryzae could preferentially control gluconeogenesis. The addition of 0.1 M glucose under gluconeogenic culture conditions also showed that Fru-2,6-P2 fluctuations appeared to be, at least in short term, more closely related to temporal changes in the hexose-6-phosphate concentration.Key words: Aspergillus oryzae; fructose-2,6-bisphosphate; phosphofructokinase II (PFK II); cyclic AMP; gluconeogenesis control.

Research on the Characteristics and Culture Conditions of Saccharomyces boulardii

2011 ◽  
Vol 343-344 ◽  
pp. 594-598
Author(s):  
Li Ping Du ◽  
Rui Xue Hao ◽  
Dong Guang Xiao ◽  
Li Li Guo ◽  
Wei Dong Gai
Keyword(s):  
Ph Value ◽  
Acid Tolerance ◽  
Saccharomyces Boulardii ◽  
Culture Conditions ◽  
Inoculum Size ◽  
Flask Culture ◽  
Culture Temperature ◽  
Highly Active ◽  
Physiological And Biochemical Characteristics ◽  
Thermal Death

The morphological, physiological and biochemical characteristics of Saccharomyces boulardii were studied for the preparation of highly active yeast. The optimal condition of the flask culture including inoculum size, culture temperature, pH value, were investigated separately. The results showed that S.boulardii was asporogenous and had better heat tolerance and acid tolerance growing well at 37°C and pH 2.0. We also found that thermal death temperature was 55~56°C and S.boulardii is well ethanol-tolerant, for the highest concentration of ethanol that S.boulardii can tolerate was 20%. The optimal culture conditions in a flask culture was YEPD medium with 50ml/250ml medium volume, natural pH, 5% inoculum size, 32°C culture temperature, 160 r/min shaker speed, and 24 hours cultivation period. Under these conditions, suspension was diluted 10 times and the optical density at 600 nm was up to 0.85.

scholarly journals Biosynthesis of Red Elemental Selenium Protein

2019 ◽  
Vol 131 ◽  
pp. 01011
Author(s):  
Guojie Wu ◽  
Xuegui Lin ◽  
Yong Liu ◽  
Jinwen Peng ◽  
Jinhui Huang
Keyword(s):  
Sodium Selenite ◽  
Orthogonal Test ◽  
Elemental Selenium ◽  
Fermentation Condition ◽  
Selenium Content ◽  
Culture Temperature ◽  
Culture Time ◽  
Condition Optimization ◽  
Active Dry Yeast ◽  
Single Factor Experiment

The red elemental selenoprotein was biosynthesized by active dry yeast in this study. Through a single factor experiment and L9(34)-orthogonal test fermentation condition optimization, the selenium content was 15.72 mg/g under optimal condition. The optimum culture condition was as follows: the sodium selenite concentration (0.8 mg/mL), culture time (36 h), the yeast dosage (5 g), pH (5.5), culture temperature (30°C), and the medium Baume degree (8 °Bé). The analysis of amino acid species, content and morphology of the product showed that the red elemental selenoprotein was successfully synthesized.

scholarly journals Optimization of Culture Conditions for Amoxicillin Degrading Bacteria Screened from Pig Manure

2020 ◽  
Vol 17 (6) ◽  
pp. 1973 ◽  
Author(s):  
Xuanjiang Yang ◽  
Panpan Guo ◽  
Miao Li ◽  
Hualong Li ◽  
Zelin Hu ◽  
...  
Keyword(s):  
Response Surface ◽  
Ph Value ◽  
Culture Conditions ◽  
Pig Manure ◽  
Bacterial Strains ◽  
Inoculum Level ◽  
Fermentation Conditions ◽  
Culture Time ◽  
Degrading Bacteria ◽  
Initial Ph

(1) Objective: The objective of this study was to screen amoxicillin (AMX)-degrading bacterial strains in pig manure and optimize the fermentation conditions for these strains to achieve high fermentation rate, which can provide an effective way for the practical application of bacterial strains as antibiotic-degrading bacterial in treating livestock waste for antibiotic residues. (2) Methods: Antibiotic susceptibility tests and high-performance liquid chromatography tandem mass spectrometry (HPLC-MS/MS) were employed to screen AMX-degrading bacterial strains in pig manure. The culture conditions were optimized for AMX-degrading bacterial strains using Plackeet–Burman design (PBD), the steepest ascent design, and the response surface methods, coupled with the Box–Behnken design (BBD). The effects of culture time, temperature, rotator (mixing) speed, inoculum level, and initial pH value on the growth of AMX-degrading strains were investigated. Experimental data obtained from BBD were utilized to generate a second-order polynomial regression model for evaluating the effects of the tested variables on the optical density at 600 nm (OD600) of culture solutions as the growth indicator for the screened AMX-degrading strains. (3) Results: The initial pH, culture time, and the inoculum level had significant effects on the OD600 value (growth) of the screened AMX-degrading strains. The initial pH value was found to be the most critical factor influencing the growth of bacteria. The optimized culture condition for the bacterial growth determined by the response surface methodology was: the initial pH of 6.9, culture time of 52 h, and inoculum level of 2%. The average OD value of 12 different fermentation conditions in the initial fermentation tests in this study was 1.72 and the optimization resulted in an OD value of 3.00. The verification experiment resulted in an OD value of 2.94, which confirmed the adequacy of the optimization model for the determining the optimal culture condition. (4) Conclusions: The growth of the screened strain of AMX-degrading bacteria could be optimized by changing the fermentation conditions. The optimization could be achieved by using the Box–Behnken response surface method and Plackett–Burman experimental design.

scholarly journals Globin synthesis in erythroid bursts that mature sequentially in culture. I. Studies in cultures of adult peripheral blood BFU-Es

Blood ◽  
1981 ◽  
Vol 58 (5) ◽  
pp. 969-974 ◽  
Author(s):  
T Papayannopoulou ◽  
B Nakamoto ◽  
S Kurachi ◽  
G Stamatoyannopoulos
Keyword(s):  
Peripheral Blood ◽  
Terminal Differentiation ◽  
Fetal Hemoglobin ◽  
Culture Conditions ◽  
Third Culture ◽  
Hemoglobin Synthesis ◽  
The Third ◽  
Culture Time ◽  
Globin Synthesis ◽  
Adult Peripheral Blood

Abstract To study whether the culture time at which the burst populations mature influences the expression of fetal hemoglobin in bursts, we measured hemoglobin synthesis in cohorts of fully hemoglobinized erythroid bursts maturing sequentially in cultures of adult peripheral blood BFU- Es. In 13 of 15 experiments, a decline in gamma/gamma + beta ratio was noted as the culture time advanced. On the average, erythroid bursts that mature during the third culture week showed lower levels of fetal Hb synthesis compared to bursts that are already mature in the second culture week. The decline of gamma/gamma + beta ratio with culture time was also noted in erythroid bursts composed of immature erythroblasts. The enhanced HbF formation in peripheral blood BFU-E cultures is thus most pronounced among the bursts that become hemoglobinized early, and there is a tendency for normalization of HbF synthesis in bursts that mature in late culture days. These results can be interpreted by several alternatives, including the possibility that the expression of high HbF levels in the early days of adult BFU-E cultures is a reflection of premature commitment to terminal differentiation of progenitors that possess an active HbF program. The present data indicate that the variation of HbF synthesis with culture time should be taken into consideration when the influence of various culture conditions of HbF synthesis is studied in BFU-E cultures.

Sign in / Sign up

Export Citation Format

Share Document