Solid State Fermentation for Chicken Manure Regenerative Feed Containing Poly-γ-Glutamic Acid

2013 ◽  
Vol 807-809 ◽  
pp. 1176-1180
Author(s):  
Xiong Chen ◽  
Qi Yuan Li ◽  
Jun Dai ◽  
Zhi Wang
Keyword(s):  
Solid State ◽  
Glutamic Acid ◽  
Scale Up ◽  
Initial Moisture Content ◽  
Chicken Manure ◽  
Feed Additive ◽  
Inoculum Level ◽  
Initial Ph ◽  
Late Model ◽  
Soybean Cake

Experiments were made to investigate the technology and conditions for producing chicken manure regenerative feed containing poly-γ-glutamic acid (γ-PGA) under solid-state fermentations (SSF) fromBacillussubtilisCCTCC202048. The maximum γ-PGA production (5.4%) was obtained in the mixed substrates of chicken manure, soybean cake and wheat bran (1:1:0.2 w/w) supplemented with 0.5% glutamic acid and 0.5% citric acid, with initial moisture content 65% and initial pH 8.5 in 250 ml flasks, inoculation at mid-log phase with a 4% inoculum level and cultivation for 48 h at 37°C. Scale-up experiments on the scale of 130 kg also showed that the yield of γ-PGA reached 4.2% and fermentation increased protein content of the solid substrates from 17.5% to 32.8%. These would lay a foundation for lessening the pollution of chicken manure, increasing chicken manure feed efficiency and exploring a late-model feed additive.

SUBMERGED CITRIC ACID FERMENTATION OF SUGAR BEET MOLASSES: INCREASE IN SCALE

1955 ◽  
Vol 1 (5) ◽  
pp. 299-311 ◽  
Author(s):  
R. Steel ◽  
C. P. Lentz ◽  
S. M. Martin
Keyword(s):  
Citric Acid ◽  
Scale Up ◽  
Aeration Rate ◽  
Small Scale ◽  
Acid Fermentation ◽  
Inoculum Level ◽  
Cross Sectional ◽  
Factors Affecting ◽  
Beet Molasses ◽  
Initial Ph

Factors affecting the production of citric acid in the submerged fermentation of ferrocyanide-treated beet molasses by Aspergillus niger were studied in 2.5 and 36 liter fermenters. The small fermenters were used to determine the effects of changes in sterilization technique, phosphate supplement, ferrocyanide treatment, inoculum level, initial pH, fermentation temperature, and aeration rate. The relation between ferrocyanide concentration and inoculum level was also studied. Four different samples of molasses were fermented successfully. An average yield of 8.2% citric acid (64% conversion) was obtained from 51 small-scale fermentations. Comparable yields were obtained in the large fermenters under comparable conditions. Most of the information obtained with the small fermenters was applicable to the larger-scale fermenters, but in the latter the fermentation was significantly more stable. Aeration was the main problem in the scale-up and aeration rates approximately double those calculated on a fermenter cross-sectional area basis were required for comparable results in the large fermenters.

Cultural conditions optimization for production of β-galactosidase from Bacillus licheniformis ATCC 12759 under solid-state fermentation

2018 ◽  
Vol 43 (3) ◽  
pp. 240-247 ◽  
Author(s):  
Nurullah Akcan
Keyword(s):  
Solid State ◽  
Moisture Content ◽  
Bacillus Licheniformis ◽  
Solid State Fermentation ◽  
Incubation Temperature ◽  
Fermentation Medium ◽  
Inoculum Level ◽  
Cultural Conditions ◽  
Initial Ph ◽  
Fermentation Parameters

AbstractObjective:The aim of this work was to study the optimal cultivation conditions for β-galactosidase production byBacillus licheniformisATCC 12759.Materials and methods:The screening of β-galactosidase production fromB. licheniformisATCC 12759 was performed by solid state fermentation method on media rich with rice bran (RB). Different factors were tested for the optimization of β-galactosidase production.Results:Certain fermentation parameters involving incubation time, incubation temperature, inoculum level, moisture content, initial pH, agitation speed, size of fermentation medium and optimum temperature of β-galactosidase activity were studied separately. Maximal amount of β-galactosidase production was obtained when solid-state fermentation (SSF) was carried out using RB, having inoculum level 35%, moisture content of 20%, initial pH 7.5 at 37°C for 48 h.Conclusion:Results indicated that optimal fermentation conditions play a key role in the maximum production of β-galactosidase fromB. licheniformisATCC 12759. This study shows the potential of the studied enzymes to be promoting candidates for the degradation of lactose and production of important bioproducts.

Control of Nitrogen Loss during Co-Composting of Banana Stems with Chicken Manure

2011 ◽  
Vol 295-297 ◽  
pp. 773-776 ◽  
Author(s):  
Chun Yuan Wu ◽  
Qin Fen Li ◽  
Yu Bai Zhang
Keyword(s):  
Moisture Content ◽  
Nitrogen Source ◽  
Nitrogen Loss ◽  
Initial Moisture Content ◽  
Chicken Manure ◽  
Nitrogen Accumulation ◽  
Paddy Straw ◽  
N Loss ◽  
Initial Ph ◽  
Neutral Conditions

This work studied the effect of basic composting parameters (C/N ratio, turning frequency and moisture), initial pH, nitrogen source and fermenting agent on nitrogen loss (N-loss) during co-composting of banana stems with chicken manure in order to determine the best composting conditions. The experimental results suggested that co-compost with minimum N-loss entails operating at turning frequency with once two days, initial moisture content of 65% and C/N ratio of 25. NH3 volatilization and N-loss were weakened under neutral conditions. Moreover, the effect of additive organic wastes and fermenting agents on N-loss during the banana stems-chicken manure co-composting was significantly different: adding bagasse or fermenting agent M-1 could effectively reduce the release of nitrogen, promotes the nitrogen accumulation, and lead to the increase of total nitrogen (TN) by 6.1 % and 6.5 %, respectively; while N-loss was enhanced with addition of paddy straw and fermenting agent M-2, with TN decreasing rate of 11.5 % and 10.9 %.

Scale-Up Production of Bacillus Thuringiensis by Box-Type Solid-State Fermentation Equipment

2013 ◽  
Vol 864-867 ◽  
pp. 299-302
Author(s):  
Li Na Qiu ◽  
Wei Wei Zhang ◽  
Ai Jun Gong ◽  
Ru Lei Yao
Keyword(s):  
Solid State ◽  
Bacillus Thuringiensis ◽  
Solid State Fermentation ◽  
Large Scale ◽  
Ph Value ◽  
Scale Up ◽  
Initial Moisture Content ◽  
Solid Substrate ◽  
Microbial Pesticides ◽  
Result Show

The Bacillus thuringiensis (Bt) which is considered as the most effective bio-pesticide is widely used in the world. There are mainly two modes for the production of this microbial pesticides: submerged fermentation and solid-state fermentation. In this paper, The Scale-up of Kg-class Bt production was studied on wheat bran based media in the Box-type solid-state fermentation equipment made by our lab. The fermentation conditions were optimized and the result show that, Bt production yielded a significant increase in spore count and toxin content at the following conditions: actiactivation of culture for 8h, initial moisture content of solid substrate 55%, pH value 7.5, keeping the thick of inoculated media about 4cm and fermenting for 45 h, Maintaining the temperature within a range of 25-30°C, The bioassay efficacy of the product could be above 13000IU·mg-1, The production of Bt kilograms class by solid-state fermentation was successfully represented as well as providing a strong technical support to the large-scale industrial production of Bt bio-pesticide.

scholarly journals Optimization of Solid State Fermentation Condition to Increase Total Phenolic Content and Antioxidant Activity in Seaweed (Kappaphycus Alvarezii) Extract

2019 ◽  
Vol 7 (4.14) ◽  
pp. 257
Author(s):  
Norakma M.N ◽  
Zaibunnisa A.H ◽  
Lai L.W ◽  
Givitha R ◽  
Wan Razarinah W. A. R
Keyword(s):  
Antioxidant Activity ◽  
Solid State ◽  
Moisture Content ◽  
Phenolic Content ◽  
Kappaphycus Alvarezii ◽  
Initial Moisture Content ◽  
Total Phenolic ◽  
Scavenging Activity ◽  
Fermentation Condition ◽  
Inoculum Level

Conventional techniques such as solvent extraction can be effectively used to extract free phenolic compounds in plants. However, this method is inefficient to extract bound phenolics. Solid state fermentation (SSF) approach with Aspergillus oryzae was used to enhance bioavailability of polyphenols in Kappaphycus alvarezii. A set of experiment was computed by face centered central composite design (FCCCD) to optimize the fermentation parameters based on maximum phenolic content and antioxidant activity. Four independent variables namely: time (0, 4 and 8 days), temperature (28, 30 and 32 °C), initial moisture content (60, 70 and 80 %) and inoculum level (10, 20 and 30 % (v/v)) were investigated. The experimental results for both TPC and DPPH were 9.449 ± 0.198 mg GAE/g and 87.135 ± 0.857 % of scavenging activity, respectively; where both responses were in good agreement with RSM model prediction. The RSM design used has been proven to successfully predict the total phenolic content and antioxidant activity. Fermentation condition with 70% initial moisture content, 10% (v/v) inoculum level, performed at 30oC for 4 days was found to produce maximum TPC and DPPH radical scavenging activity of Kappaphycus alvarezii.  

scholarly journals Production of L-glutaminase From Agro-industrial Waste by Halomonas Elongata MM-5 Under Solid State Fermentation

2021 ◽  
Author(s):  
Mohammed Mujahed ◽  
Dhiraj G Jirgale ◽  
Basavraj M Kareppa
Keyword(s):  
Solid State ◽  
Moisture Content ◽  
Solid State Fermentation ◽  
Maximum Yield ◽  
Initial Moisture Content ◽  
Nitrogen Sources ◽  
Malt Extract ◽  
Inoculum Level ◽  
Bengal Gram ◽  
Halomonas Elongata

Abstract In this study L-glutaminase production by extremely halotolerant Halomonas elongata MM-5 using solid-state fermentation was investigated. Screening of a variety of agro-industrial byproducts such as rice husk, green gram husk, bengal gram husk, red gram husk, safflower oil cake, groundnut oil cake, black gram husk, groundnut skin and wheat bran was carried out individually and in different combinations. Optimization of various physicochemical parameters namely incubation time, pH, temperature, initial moisture content, carbon sources, nitrogen sources, L-glutamine concentration and inoculum level was carried out. Among the various substrates screened individually red gram husk supported the maximum production of enzyme 79.03±0.49 IU/gds. In the mixture of substrates screened red gram husk and bengal gram husk in the ratio (60:40) showed maximum 92.06±1.42 IU/gds enzyme production. The L-glutaminase production was maximum after 4 days of incubation period, pH 8, temperature 40°C and 80 percent moisture content. The lactose and malt extract used as carbon and nitrogen sources respectively supported the maximum yield of L-glutaminase. After screening and optimization of various parameters, the yield of L-glutaminase increased from 79.03 to 159.12 IU/gds.

scholarly journals Erratum to: Optimization of Xylanase Production by Filamentous Fungi in Solid-State Fermentation and Scale-up to Horizontal Tube Bioreactor

2015 ◽  
Vol 176 (1) ◽  
pp. 317-319 ◽  
Author(s):  
N. Pérez-Rodríguez ◽  
F. Oliveira ◽  
B. Pérez-Bibbins ◽  
I. Belo ◽  
A. Torrado Agrasar ◽  
...  
Keyword(s):  
Solid State ◽  
Filamentous Fungi ◽  
Solid State Fermentation ◽  
Scale Up ◽  
Horizontal Tube ◽  
Xylanase Production

scholarly journals Lovastatin Production byAspergillus terreusUsing Agro-Biomass as Substrate in Solid State Fermentation

2012 ◽  
Vol 2012 ◽  
pp. 1-11 ◽  
Author(s):  
Mohammad Faseleh Jahromi ◽  
Juan Boo Liang ◽  
Yin Wan Ho ◽  
Rosfarizan Mohamad ◽  
Yong Meng Goh ◽  
...  
Keyword(s):  
Particle Size ◽  
Solid State ◽  
Moisture Content ◽  
Nitrogen Source ◽  
Solid State Fermentation ◽  
Incubation Temperature ◽  
Initial Moisture Content ◽  
Fermentation Time ◽  
Maximum Production ◽  
Additional Nitrogen

Ability of two strains ofAspergillus terreus(ATCC 74135 and ATCC 20542) for production of lovastatin in solid state fermentation (SSF) using rice straw (RS) and oil palm frond (OPF) was investigated. Results showed that RS is a better substrate for production of lovastatin in SSF. Maximum production of lovastatin has been obtained usingA. terreusATCC 74135 and RS as substrate without additional nitrogen source (157.07 mg/kg dry matter (DM)). Although additional nitrogen source has no benefit effect on enhancing the lovastatin production using RS substrate, it improved the lovastatin production using OPF with maximum production of 70.17 and 63.76 mg/kg DM forA. terreusATCC 20542 andA. terreusATCC 74135, respectively (soybean meal as nitrogen source). Incubation temperature, moisture content, and particle size had shown significant effect on lovastatin production (P<0.01) and inoculums size and pH had no significant effect on lovastatin production (P>0.05). Results also have shown that pH 6, 25°C incubation temperature, 1.4 to 2 mm particle size, 50% initial moisture content, and 8 days fermentation time are the best conditions for lovastatin production in SSF. Maximum production of lovastatin using optimized condition was 175.85 and 260.85 mg/kg DM forA. terreusATCC 20542 and ATCC 74135, respectively, using RS as substrate.

scholarly journals Glutamic Acid Residues of Bacteriorhodopsin at the Extracellular Surface as Determinants for Conformation and Dynamics as Revealed by Site-Directed Solid-State 13C NMR

2004 ◽  
Vol 86 (3) ◽  
pp. 1673-1681 ◽  
Author(s):  
Hazime Saitô ◽  
Satoru Yamaguchi ◽  
Keiji Ogawa ◽  
Satoru Tuzi ◽  
Mercedes Márquez ◽  
...  
Keyword(s):  
Solid State ◽  
Glutamic Acid ◽  
13C Nmr ◽  
Solid State 13C Nmr
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