Isolation and Characterization of Actinomycetes from Acidic Cultures of Ores and Concentrates

2013 ◽  
Vol 825 ◽  
pp. 406-409
Author(s):  
Akemi Matsubara ◽  
Jasmin E. Hurtado
Keyword(s):  
Heavy Metals ◽  
Sodium Chloride ◽  
Environmental Pollution ◽  
Ferrous Iron ◽  
Iron Oxidation ◽  
Mining Industry ◽  
Thiosulfate Oxidation ◽  
Ph Tolerance ◽  
Isolation And Characterization

Mining industry is a source of wealth but also of environmental pollution in Peru. In this study 12 colonies of actinomycetes were isolated in acidic cultures from mineral ores and concentrates from mines of the Peruvian highlands. The isolates were characterized phenotypically by microscopy and growth at different conditions as pH tolerance, temperature, and sodium chloride, heavy metals resistance; ferrous iron and thiosulfate oxidation. All isolates were identified as actinomycetes based on their cultural and spore characteristics. Most of the isolates were able to grow at 8 - 45°C and pH 4 - 11. 60% of isolates grew at 10% NaCl but none of them growth at 13%. Iron oxidation was shown by 60% of isolates at pH 4, but only 25% were able to oxidize iron at pH 2. Thiosulfate oxidation was not detected in any isolate. Most of the isolates showed capacity to grow in medium with 200 ppm of Pb, Fe, Zn and 100 ppm of Cu. All of the physiological characteristics found in this work indicate the potential of these isolates as source for bioremediation and bioleaching.

Microorganisms Oxidize Iron (II) Ions in the Presence of High Concentrations of Sodium Chloride - Potentially Useful for Bioleaching

2017 ◽  
Vol 262 ◽  
pp. 364-367 ◽  
Author(s):  
Dieu Huynh ◽  
Stefan Kaschabek ◽  
Wolfgang Sand ◽  
Michael Schlömann
Keyword(s):  
Sodium Chloride ◽  
Ferrous Iron ◽  
High Salinity ◽  
Sea Water ◽  
Iron Oxidation ◽  
Ore Deposit ◽  
Enrichment Cultures ◽  
Spanish Coast ◽  
High Concentrations ◽  
Microbial Groups

Acidophilic leaching microorganisms have been reported to be in general intolerant to high salinity, namely high concentrations of chloride. At present this restriction hampers the use of sea water for bioleaching technology. Enrichment cultures obtained in this study from a former ore deposit near the Spanish coast oxidize ferrous iron in the presence of up to 50 gL-1 NaCl at pH 2.5 and 37°C. The presence of at least 5 gL-1 NaCl was shown to be an obligate requirement for iron oxidation. The major microbial groups comprise Alicyclobacillus and Arthrobacter. The findings may be of biotechnological relevance.

Isolation and characterization of heavy-metal-mobilizing bacteria from contaminated soils and their potential in promoting Pb, Cu, and Cd accumulation by Coprinus comatus

2012 ◽  
Vol 58 (1) ◽  
pp. 45-53 ◽  
Author(s):  
Xiao-bing Jing ◽  
Nan He ◽  
Ying Zhang ◽  
Yan-ru Cao ◽  
Heng Xu
Keyword(s):  
Heavy Metals ◽  
Heavy Metal ◽  
Contaminated Soils ◽  
Bacillus Sp ◽  
Cd Accumulation ◽  
Treated Soil ◽  
Coprinus Comatus ◽  
Leading Role ◽  
Isolation And Characterization

The enhanced effect of heavy-metal-mobilizing bacteria on the uptake of Pb, Cu, and Cd by Coprinus comatus from Pb-, Cu-, and Cd-multicontaminated soil was assessed in this study. Thirteen strains, tolerating 800 mg·L–1 Pb, 200 mg·L–1 Cu, and 200 mg·L–1 Cd simultaneously were selected for heavy-metal-solubilizing experiments in soil. The mobilization of heavy metals depended on the characteristics of bacteria and heavy metals. Correlation analysis demonstrated that for Pb solubilization, the acid-producing ability was the most significant factor, while for Cu and Cd, siderophores played a leading role in this process. Four strains, based on their excellent ability to solubilize heavy metal in soil, were applied in pot experiments. The results showed that all strains can promote the growth of C. comatus and meanwhile help mushroom accumulate more heavy metals (Pb, Cd, and Cu). The maximum uptake for total Pb and Cu by C. comatus was observed in inoculations with Bacillus sp. strain JSG1 (2.02- and 2.13-fold, respectively, compared with uninoculated soil), while for Cd, it was recorded in Bacillus sp. strain PB2 treated soil (2.03-fold). Therefore, this work suggests that the mushroom–bacteria interaction can be developed into a novel bioremediation strategy.

The Effect of Streptomyces sp. Isolated from Acidic Cultures of Minerals on Plant Development in Environments Polluted with Mercury

2015 ◽  
Vol 1130 ◽  
pp. 598-601
Author(s):  
Sara Liz Pacheco ◽  
Patricia Sheen ◽  
Jasminc Hurtado
Keyword(s):  
Heavy Metals ◽  
Sodium Chloride ◽  
Phaseolus Vulgaris ◽  
Medicago Sativa ◽  
Plant Development ◽  
Lactuca Sativa ◽  
Root Length ◽  
Streptomyces Sp ◽  
Ph Tolerance ◽  
Number Of Leaves

Three isolates ofStreptomyces variabilis(AB5, F and K1A) and one isolate ofStreptomyces sp.(C2) were isolated of acidic cultures from mineral ores and concentrates from mines of the Peruvian highlands. The isolates were identified molecular and phenotypically and characterized by growth at different conditions as pH tolerance, temperature, and sodium chloride and heavy metals resistance.Streptomyces sp. (C2) was able to grow until 200 ppm of mercury andS. variabilis(AB5) grows in the presence of 100 ppm of mercury. The isolates AB5 and K1A ofS. variabiliswere able to grow only until 50 ppm of mercury.All strains were tested by their ability to induce germination ofLactuca sativa,Medicago sativaandPhaseolus vulgarisseeds exposed to different mercury concentrations. It was shown a significative improvement inL.sativaandP. vulgaris. InL. sativaexposed to 50 ppm of mercury, all strains were able to improve germination, root length and number of leaves. Without Streptomyces addition, they only growth until 10 ppm of mercury.Streptomyces sp.isolatehad better results in germination, root length and number of leaves ofL. sativa.The results found in this work indicate the potential of these isolates for restoration of soils polluted with mercury.

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