The Effect of Streptomyces sp. Isolated from Acidic Cultures of Minerals on Plant Development in Environments Polluted with Mercury

2015 ◽  
Vol 1130 ◽  
pp. 598-601
Author(s):  
Sara Liz Pacheco ◽  
Patricia Sheen ◽  
Jasminc Hurtado
Keyword(s):  
Heavy Metals ◽  
Sodium Chloride ◽  
Phaseolus Vulgaris ◽  
Medicago Sativa ◽  
Plant Development ◽  
Lactuca Sativa ◽  
Root Length ◽  
Streptomyces Sp ◽  
Ph Tolerance ◽  
Number Of Leaves

Three isolates ofStreptomyces variabilis(AB5, F and K1A) and one isolate ofStreptomyces sp.(C2) were isolated of acidic cultures from mineral ores and concentrates from mines of the Peruvian highlands. The isolates were identified molecular and phenotypically and characterized by growth at different conditions as pH tolerance, temperature, and sodium chloride and heavy metals resistance.Streptomyces sp. (C2) was able to grow until 200 ppm of mercury andS. variabilis(AB5) grows in the presence of 100 ppm of mercury. The isolates AB5 and K1A ofS. variabiliswere able to grow only until 50 ppm of mercury.All strains were tested by their ability to induce germination ofLactuca sativa,Medicago sativaandPhaseolus vulgarisseeds exposed to different mercury concentrations. It was shown a significative improvement inL.sativaandP. vulgaris. InL. sativaexposed to 50 ppm of mercury, all strains were able to improve germination, root length and number of leaves. Without Streptomyces addition, they only growth until 10 ppm of mercury.Streptomyces sp.isolatehad better results in germination, root length and number of leaves ofL. sativa.The results found in this work indicate the potential of these isolates for restoration of soils polluted with mercury.

Isolation and Characterization of Actinomycetes from Acidic Cultures of Ores and Concentrates

2013 ◽  
Vol 825 ◽  
pp. 406-409
Author(s):  
Akemi Matsubara ◽  
Jasmin E. Hurtado
Keyword(s):  
Heavy Metals ◽  
Sodium Chloride ◽  
Environmental Pollution ◽  
Ferrous Iron ◽  
Iron Oxidation ◽  
Mining Industry ◽  
Thiosulfate Oxidation ◽  
Ph Tolerance ◽  
Isolation And Characterization

Mining industry is a source of wealth but also of environmental pollution in Peru. In this study 12 colonies of actinomycetes were isolated in acidic cultures from mineral ores and concentrates from mines of the Peruvian highlands. The isolates were characterized phenotypically by microscopy and growth at different conditions as pH tolerance, temperature, and sodium chloride, heavy metals resistance; ferrous iron and thiosulfate oxidation. All isolates were identified as actinomycetes based on their cultural and spore characteristics. Most of the isolates were able to grow at 8 - 45°C and pH 4 - 11. 60% of isolates grew at 10% NaCl but none of them growth at 13%. Iron oxidation was shown by 60% of isolates at pH 4, but only 25% were able to oxidize iron at pH 2. Thiosulfate oxidation was not detected in any isolate. Most of the isolates showed capacity to grow in medium with 200 ppm of Pb, Fe, Zn and 100 ppm of Cu. All of the physiological characteristics found in this work indicate the potential of these isolates as source for bioremediation and bioleaching.

scholarly journals Morphological and Physiological Screening of Aerial Yam (Dioscorea bulbifera L.) for Sodium Chloride Tolerance

2021 ◽  
pp. 44-52
Author(s):  
S. E. Kalu ◽  
A. N. Osuagwu ◽  
I. A. Ekpo ◽  
L. E. Okonko ◽  
G. M. Ubi
Keyword(s):  
Salt Stress ◽  
Sodium Chloride ◽  
Chlorophyll A ◽  
Root Length ◽  
Stress Conditions ◽  
Morphological Data ◽  
Chlorophyll B ◽  
Number Of Leaves ◽  
Dioscorea Bulbifera ◽  
Glucose 6 Phosphate Dehydrogenase

Aerial yam (Dioscorea bulbifera L.) is an indigenous, edible and energy-giving crop used for herbal remedy for some common ailments like diabetes, dysentery, cholera and conjunctivitis. It is fast-growing and can adapt to different soil types and habitat conditions, therefore seen as having the potential of tolerating salt stress conditions in this area of climate change. This study, therefore, sought to determine the tolerance of D. bulbifera to salt stress conditions through morphological and physiological studies. Bulbils of D.bulbiferaof two distinguished shapes were sourced from local farmers in Abia State, AkwaIbom State and Cross River State of the Niger- Delta region of Nigeria, and established in Biological Sciences Research Farm, University of Calabar, Calabar, to have the various lines for the experiment. Randomized Complete Block Design (RCBD) with three replications was used for the study. At four weeks after sprouting, the plants were treated with 250ml of different concentrations of sodium chloride (0, 100, 200 and 300mM) twice a week for six weeks. Morphological data of leaf color, number of leaves/plant, root length, leaf length, petiole length, leaf area, vine length and number of bulbils were measured weekly during the treatment period while physiological data of Chlorophyll-a and chlorophyll- b concentrations, peroxidase and glucose-6-phosphate dehydrogenase activities were analyzed two weeks after the completion of the treatment. Results revealed that sodium chloride reduced the vine length, number of leaves, number of bulbils, chlorophyll-a and chlorophyll-b concentrations of the plant, and caused an increase in the root length, peroxidase and glucose-6- phosphate dehydrogenase activities. Although sodium chloride reduced the growth of the D.bulbifera, all the lines were able to produce bulbils at a salt concentration of 0.00mM to 200mM. Therefore, this plantcould be regarded as a moderate salt tolerant plant. Significant differences in growth and yield were found between the lines (P<0.05). Bulbils with round shape tended to show more growth than the spindle shape.

Phytoremediation potential of Nauclea diderrichii (De Wild and Th. Dur.) seedlings grown in spent engine oil contaminated soil

2020 ◽  
Vol 55 (4) ◽  
pp. 261-272
Author(s):  
OI Bolanle Ojo ◽  
MKC Sridhar
Keyword(s):  
Heavy Metals ◽  
Contaminated Soil ◽  
Root Length ◽  
Engine Oil ◽  
Lead And Cadmium ◽  
Significant Difference ◽  
Spent Engine Oil ◽  
Number Of Leaves ◽  
Collar Diameter ◽  
Nauclea Diderrichii

Remediation of environmental pollution has been of great concern in recent years. This study was carried out to investigate the potentials of Nauclea diderrichii seedlings to remediate heavy metals from spent engine oil (SEO) contaminated soil. SEO from diesel and petrol engines in different quantities were applied to each plant using 2 kg soil. Plant height, collar diameter, number of leaves, root length were assessed. Amount of lead and cadmium present in the leaves, stem and root of the seedlings were assessed at the end of the experiment. Result showed that 100 % of the seedlings subjected to various treatments survived for the duration of 5 months. There was no significant difference (α>0.05) in the height of N. diderrichii seedlings. Significant (α=0.05) negative reductions were observed in the seedlings collar diameter, no. of leaves and root length. There was significant difference (α>0.05) in the amount of lead and cadmium extracted by N. diderrichii seedlings. This study has been able to show that N. diderrichii has the ability/potential to phyto-extract heavy metals from soil. Bangladesh J. Sci. Ind. Res.55(4), 261-272, 2020

Inhibition of the catalase activity from Phaseolus vulgaris and Medicago sativa by sodium chloride

2007 ◽  
Vol 45 (8) ◽  
pp. 535-541 ◽  
Author(s):  
Noel A. Tejera García ◽  
Carmen Iribarne ◽  
Francisco Palma ◽  
Carmen Lluch
Keyword(s):  
Sodium Chloride ◽  
Phaseolus Vulgaris ◽  
Medicago Sativa ◽  
Catalase Activity

Subsitusi Fitohormon Dengan Air Kelapa (Cocos nucifera L.) pada Medium Vacin and Went Terhadap Pertumbuhan Eksplan Anggrek Dendrobium sp Secara in Vitro

2021 ◽  
Vol 3 (2) ◽  
Author(s):  
Wulan Dari Neng Gumiwang ◽  
Tintrim Rahayu ◽  
Ari Hayati
Keyword(s):  
Root Length ◽  
Cocos Nucifera ◽  
Water Concentration ◽  
Coconut Water ◽  
Culture Bottle ◽  
Randomized Design ◽  
Number Of Leaves ◽  
Completely Randomized Design ◽  
Cocos Nucifera L

The purpose of this research is to determine the concentration of young coconut water that is appropriate for the growth of orchid plantlets (Dendrobium sp.) In vitro. This study used an experimental method, descriptive data analysis to compare several different concentrations of coconut water. The design of this study uses a completely randomized design (CRD). The treatments consist of 0% coconut water concentration (as a control), 15%, 30% and 60%. Each concentration was carried out 5 replications and each repetition consisted of 5 Dendrobium sp plantlets in each culture bottle conducted for 40 HST, for observing the root length carried out for 50 HST. The highest number of shoots and leaves were produced at the same concentration, namely 150 ml / L coconut water treatment (15% concentration) with an average of 2.8 shoots and the average number of leaves 10.8 leaves. The average number of roots and the longest root length was produced at a concentration of 600 ml / L coconut water (60% concentration) with an average of 6 roots, and the longest root length was 0.5 cm.Keywords: Young coconut water, (Cocos nucifera L.), Dendrobium sp., in vitro, growth.ABSTRAKTujuan penelitian ini ialah menentukan konsentrasi air kelapa muda yang tepat untuk pertumbuhan planlet anggrek (Dendrobium sp.) secara in vitro. Penelitian ini menggunakan metode eksperimen, analisis data secara deskriptif untuk membandingan beberapa konsentrasi air kelapa yang berbeda. Rancangan penelitian ini menggunakan Rancangan Acak Lengkap (RAL). Perlakukan terdiri dari konsentrasi air kelapa 0 % (sebagai kontrol), 15% , 30% dan 60%. Masing-masing konsentrasi dilakukan 5 kali ulangan dan setiap ulangan terdiri dari 5 planlet Dendrobium sp dalam setiap botol kultur yang dilakukan selama 40 HST, untuk pengamatan panjang akar dilakukan selama 50 HST. Jumlah tunas dan jumlah daun terbanyak dihasilkan pada konsentrasi yang sama, yaitu perlakuan air kelapa 150 ml/L (konsentrasi 15%)  dengan rata-rata jumlah tunas terbanyak 2,8 tunas dan rata-rata jumlah daun terbanyak 10,8 helai daun. Rata-rata jumlah akar terbanyak dan panjang akar terpanjang dihasilkan pada konsentrasi air kelapa 600 ml/L (Konsentrasi 60%) dengan rata-rata jumlah akar terbanyak sebanyak 6 akar, dan rata-rata panjang akar terpanjang 0,5 cm.Kata kunci : Air kelapa Muda (Cocos nucifera L.), Dendrobium sp., in vitro, pertumbuhan 

GROWTH RESPONSE OF BEHA GRASS (Brachiaria humidicola) FERTILIZED WITH GOAT MANURE

2021 ◽  
Vol 2 (3) ◽  
Author(s):  
Budiman Budiman ◽  
Natsir Sandiah ◽  
La Malesi
Keyword(s):  
Plant Height ◽  
Root Length ◽  
New Production ◽  
Brachiaria Humidicola ◽  
Randomized Design ◽  
Length Data ◽  
Number Of Leaves ◽  
Completely Randomized Design ◽  
Goat Manure ◽  
Difference Test

This study aimed to determine the best dosage of goat manure as a natural fertilizer on Beha grass(Brachiaria humidicola). This study used 64 poles of Beha grass grown in polybags divided into 16plots, and it used a completely randomized design (CRD) to analyze the data. This study consist of 4treatments that is P0 (0 ton/ha of goat manure), P1 (10 ton/ha of goat manure), P2 (15 ton/ha of goatmanure), and P3 (20 ton/ha of goat manure). This study's variables were plant height, number of leaves,numbers of tillers, new production, and root length. Data analysis used ANOVA variance continued withreal honest difference test. The result of this study showed that averages of plant height (cm) wereP0=26, P1=50.5, P2=51.5, and P3=52.75. Averages of the number of leaves were P0=22, P1=110,P2=160, and P3=185. Averages of numbers of tillers were P0=7, P1=25, P2=37, and P3=38. Averages ofnew production (g) were P0=17.75, P1=123.5, P2=178, and P3=192.25. Averages of root length (cm)were P0=37.8, P1=39.5, P2=41.775, and P3=49.5. Fertilization with natural fertilizer from goat manurehas a genuine effect (P<0.01) on the growth of Beha grass. The best dosage of fertilization with goatmanure was 15 ton/ha.

scholarly journals Growth of hydroponic lettuce with optimized mineral and organomineral nutrient solutions

2017 ◽  
Vol 21 (3) ◽  
pp. 191-196
Author(s):  
Antonio F. Monteiro Filho ◽  
Márcia R. Q. A. Azevedo ◽  
Carlos A. V. de Azevedo ◽  
Josely D. Fernandes ◽  
Carisa R. da Silva ◽  
...  
Keyword(s):  
Chemical Composition ◽  
Leaf Area ◽  
Root Length ◽  
Stem Diameter ◽  
Crown Diameter ◽  
Mineral Solution ◽  
Nutrient Film Technique ◽  
Hydroponic Cultivation ◽  
Number Of Leaves ◽  
Nutrient Solutions

ABSTRACT The objective of this study was to evaluate the growth of crisp lettuce in hydroponic cultivation, following the nutrient film technique (NFT), using optimized mineral and organomineral nutrient solutions. The experiment was set in 8 x 3 factorial scheme, with treatments distributed in randomized blocks and split plots, with three replicates. The plots corresponded to eight nutrient solutions, of which four were mineral solutions with chemical composition suggested by Bernardes, Furlani, Castellane & Araújo and Ueda, and four were organomineral solutions, suggested by this research with chemical composition similar to those of the previously mentioned mineral solutions. The subplots corresponded to three varieties of crisp lettuce: Thaís, Vanda and Verônica. At 25 days after transplanting, the following parameters were evaluated: stem and crown diameter, root length, leaf area and number of leaves. For stem and crown diameters and number of leaves, the mineral solutions promoted the highest means. The mineral solution of Furlani and the organomineral solutions, except the modified solution of Castellane & Araújo, promoted the greatest root lengths. The smallest leaf area was obtained in the organomineral solution of Ueda. The cultivar Verônica had the highest root length and stem diameter.

scholarly journals Impact of sludge and wastewater on Lactuca sativa L. growth and on soil pollution

2015 ◽  
Vol 17 (1) ◽  
pp. 148-161
Keyword(s):  
Heavy Metals ◽  
Soil Pollution ◽  
Plant Height ◽  
Lactuca Sativa ◽  
Dry Matter ◽  
Municipal Wastewater ◽  
Pollution Load Index ◽  
Dry Matter Yield ◽  
Pollution Load ◽  
Treated Municipal Wastewater

<div> <p>Two greenhouse pot experiments were conducted in Agrinion, Greece. The impact of treated municipal wastewater (TMWW) and sludge (i) on the growth of <em>Lactuca sativa</em> L. var Longifolia (lettuce) and (ii) on the extent of soil pollution with heavy metals was studied. Soil pollution was assessed by calculating the Pollution Load Index (PLI). Both of these experiments were conducted, using a randomized block design in four replications and seven treatments, respectively, as follows: (a) Experiment A: study of the effect of treated municipal wastewater (TMWW): [Control, 20%, 40%, 60%, 80%, 100%, (100%+30 t/ha Sludge)] and (b) Experiment B: Study of the effect of sludge (t/ha): 0, &nbsp;6, 12,&nbsp; 18, 24 , 30, (30+100%TMWW). The sludge affected significantly plant height and fresh and dry matter yield, as well as the dry matter N content of plants, while the TMWW affected significantly the dry matter yield and non-significantly the plant height. The pollution load index (PLI) was non-significant for both treatments (sludge and TMWW). According to PLI calibration scale, the soil was found to be slightly polluted with heavy metals under both treatments.</p> </div> <p>&nbsp;</p>

scholarly journals Accumulation of heavy metals in Medicago sativa L. and Trifolium pratense L. at the contaminated fluvisol

2013 ◽  
Vol 67 (1) ◽  
pp. 95-101 ◽  
Author(s):  
Snezana Jaksic ◽  
Savo Vuckovic ◽  
Sanja Vasiljevic ◽  
Nada Grahovac ◽  
Vera Popovic ◽  
...  
Keyword(s):  
Heavy Metals ◽  
Medicago Sativa ◽  
Trifolium Pratense ◽  
Anthropogenic Impacts ◽  
Total Content ◽  
Interactive Effects ◽  
Trifolium Pratense L ◽  
Medicago Sativa L ◽  
Agricultural Areas ◽  
Toxic Concentrations

Recently, heavy metals concentrations increased in some agricultural areas due to the consequences of anthropogenic impacts. The aim of this study was to determine the level of heavy metals (As, Cr, Ni and Pb) in Medicago sativa L. and Trifolium pratense L. grown on fluvisol, in order to obtain information on safety of these nutrients. The total content of Pb, As, Cr and Ni in the samples of fluvisol was above the maximum allowable amount. The content of heavy metals in Medicago sativa L. and Trifolium pratense L. was below the critical and toxic concentrations in all samples originating from contaminated soil. It was concluded that the accumulation of heavy metals in plants did not depend only on the total content in soil, but also the affinity of the plant, and individual and interactive effects of various soil properties. No statistically significant differences in the accumulation of heavy metals between Medicago sativa L. and Trifolium pratense L were observed. It is necessary to further control of heavy metals in the investigated area, in order to prevent their entry into the food chain and provide healthy food.

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