Effect on JNK/MAPK Pathways in the Damage of Rat Testicle Sertoli Cells Induced by DBP

2013 ◽  
Vol 864-867 ◽  
pp. 797-800
Author(s):  
Huan Li ◽  
Yi Zhang ◽  
Peng Min Wang ◽  
Jing Hong Yu
Keyword(s):  
Sertoli Cells ◽  
Food Packaging ◽  
Dibutyl Phthalate ◽  
Signal Transduction Pathway ◽  
Dose Effect ◽  
Rt Pcr ◽  
Mapk Pathways ◽  
Endocrine Disrupting Compound ◽  
Endocrine Disrupting ◽  
Vitro Test

Dibutyl phthalate (DBP), an accepted environmental endocrine disrupting compound, which has anti-androgenic effects,is widely used in many industries such as plastics, pharmaceuticals, plastic food packaging materials. This article intends to study the effect on JNK/MAPK pathways in the damage of rat testicle sertoli cells induced by DBP via vitro test. Testicular sertoli cells of rates were incubated by medium containing DBP in different concentrations (0μg/ml,1μg/ml,10μg/ml, 100μg/ml). after culturing for 24 hours the expression of vimentin was detected by immunofluorescence; the expression of vimentin mRNA detected by RT-PCR; Western blot analyzed the expression of related protein of JNK/MAPK pathways in sertoli cells. The results showed that vimentin highly express in sertoli cells and gradually decrease with the increase of poisonous doses. The mRNA expression of vimentin in each exposure group has a downtrend with the increase of does. The total protein of JNK and P-JNK protein increased gradually in a dose effect manner. These results suggested that DBP can decrease the expression of vimentin mRNA, the decrease of which was regulated by JNK/MAPK signal transduction pathway that activated by DBP, and then induced the apoptosis of Sertoli cell.

scholarly journals The GNAQ T96S Mutation Affects Cell Signaling and Enhances the Oncogenic Properties of Hepatocellular Carcinoma

2021 ◽  
Vol 22 (6) ◽  
pp. 3284
Author(s):  
Eugene Choi ◽  
Sung Jean Park ◽  
Gunhee Lee ◽  
Seung Kew Yoon ◽  
Minho Lee ◽  
...  
Keyword(s):  
Hepatocellular Carcinoma ◽  
Expression Vector ◽  
Signal Transduction Pathway ◽  
Inhibitory Effect ◽  
The Cancer Genome Atlas ◽  
Treatment Modalities ◽  
Protein Signaling ◽  
Wild Type ◽  
Anchorage Independent Growth ◽  
Mapk Pathways

Hepatocellular carcinoma (HCC), the most common malignant tumor in the liver, grows and metastasizes rapidly. Despite advances in treatment modalities, the five-year survival rate of HCC remains less than 30%. We sought genetic mutations that may affect the oncogenic properties of HCC, using The Cancer Genome Atlas (TCGA) data analysis. We found that the GNAQ T96S mutation (threonine 96 to serine alteration of the Gαq protein) was present in 12 out of 373 HCC patients (3.2%). To examine the effect of the GNAQ T96S mutation on HCC, we transfected the SK-Hep-1 cell line with the wild-type or the mutant GNAQ T96S expression vector. Transfection with the wild-type GNAQ expression vector enhanced anchorage-independent growth, migration, and the MAPK pathways in the SK-Hep-1 cells compared to control vector transfection. Moreover, cell proliferation, anchorage-independent growth, migration, and the MAPK pathways were further enhanced in the SK-Hep-1 cells transfected with the GNAQ T96S expression vector compared to the wild-type GNAQ-transfected cells. In silico structural analysis shows that the substitution of the GNAQ amino acid threonine 96 with a serine may destabilize the interaction between the regulator of G protein signaling (RGS) protein and GNAQ. This may reduce the inhibitory effect of RGS on GNAQ signaling, enhancing the GNAQ signaling pathway. Single nucleotide polymorphism (SNP) genotyping analysis for Korean HCC patients shows that the GNAQ T96S mutation was found in only one of the 456 patients (0.22%). Our data suggest that the GNAQ T96S hotspot mutation may play an oncogenic role in HCC by potentiating the GNAQ signal transduction pathway.

High efficiency removal and recovery of an endocrine disrupting compound–bisphenol AF from wastewaters

2013 ◽  
Vol 116 ◽  
pp. 145-153 ◽  
Author(s):  
Lei Zhang ◽  
Junna Lv ◽  
Tianci Xu ◽  
Lijun Yang ◽  
Xiaoqing Jiang ◽  
...  
Keyword(s):  
High Efficiency ◽  
Endocrine Disrupting Compound ◽  
Endocrine Disrupting ◽  
Bisphenol Af ◽  
Removal And Recovery

Nanocomposite Membrane-Based Photocatalytic Reactor for Degradation of Endocrine-Disrupting Compound in Water

2018 ◽  
pp. 403-439
Author(s):  
Hazlini Dzinun ◽  
Mohd Hafiz Dzarfan Othman ◽  
A. F. Ismail ◽  
Mohd Hafiz Puteh ◽  
Mukhlis A. Rahman ◽  
...  
Keyword(s):  
Nanocomposite Membrane ◽  
Photocatalytic Reactor ◽  
Endocrine Disrupting Compound ◽  
Endocrine Disrupting

scholarly journals Facile Synthesis of Porous ZnO Nanoparticles Efficient for Photocatalytic Degradation of Biomass-Derived Bisphenol A Under Simulated Sunlight Irradiation

2021 ◽  
Vol 8 ◽  
Author(s):  
Yujie Wang ◽  
Kang Hu ◽  
Zhiyu Yang ◽  
Chenlu Ye ◽  
Xin Li ◽  
...  
Keyword(s):  
Bisphenol A ◽  
Photocatalytic Degradation ◽  
Inorganic Ions ◽  
Endocrine Disrupting Compounds ◽  
Degradation Process ◽  
Sunlight Irradiation ◽  
Calcination Temperatures ◽  
Endocrine Disrupting Compound ◽  
Endocrine Disrupting ◽  
Photocatalytic Removal

Bisphenol A (BPA) produced from biomass is a typical endocrine disrupting compound that is carcinogenic and genotoxic and can be accumulated in water due to its extensive use and difficult degradation. In this study, the porous ZnO photocatalyst with core-shell structure and large surface area was successfully developed for the efficient photocatalytic degradation of BPA. The various effects of calcination temperatures, BPA concentrations, ZnO dosages, pH and inorganic ions on the degradation performance were systemically studied. The results showed that 99% degradation of BPA was achieved in 1 h using the porous ZnO calcined at 550°C under the conditions of 30 mg/L BPA, 1 g/L ZnO, and pH of 6.5. Besides, the inhibition effects of anions for the photocatalytic removal of BPA decreased in the order of H2PO4- > HCO3- > SO42- > Cl−, while the cations K+, Ca2+, and Na+ had little effect on the photocatalytic degradation of BPA. The results of scavenging experiments showed that h+, ·O2-, and e− played the key role in the photocatalytic degradation process. Finally, the main pathways of BPA degradation were proposed based on ten intermediates found in the degradation process. This work may provide a good guideline to degrade various endocrine disrupting compounds in wastewater treatment.

Two endocrine disrupting dibutyl phthalate degrading esterases and their compensatory gene expression in Sphingobium sp. SM42

2015 ◽  
Vol 99 ◽  
pp. 45-54 ◽  
Author(s):  
Wirongrong Whangsuk ◽  
Pareenart Sungkeeree ◽  
Monnapa Nakasiri ◽  
Sirinthra Thiengmag ◽  
Skorn Mongkolsuk ◽  
...  
Keyword(s):  
Gene Expression ◽  
Dibutyl Phthalate ◽  
Endocrine Disrupting

scholarly journals OCTN2-mediated transport of carnitine in isolated Sertoli cells

Reproduction ◽  
2005 ◽  
Vol 129 (6) ◽  
pp. 729-736 ◽  
Author(s):  
Daisuke Kobayashi ◽  
Akihiko Goto ◽  
Tomoji Maeda ◽  
Jun-ichi Nezu ◽  
Akira Tsuji ◽  
...  
Keyword(s):  
Sertoli Cells ◽  
Pcr Analysis ◽  
Rt Pcr ◽  
Carnitine Transporter ◽  
High Affinity ◽  
Carnitine Transport ◽  
Testis Tissue ◽  
Blood Testis Barrier

Carnitine is extensively accumulated in epididymis. Carnitine is also accumulated in testis at higher concentration than in the plasma and is used in spite of the presence of the blood–testis barrier. In this study, we examined the characteristics of carnitine transport in primary-cultured rat Sertoli cells, which constitute a part of the blood–testis barrier. Uptake of [3H]carnitine (11.4 nM) from the basal side of Sertoli cells was Na+-dependent and was significantly decreased in the presence of 10 μM (48.0 ± 7.4% of control) or 100 μM unlabeled carnitine (14.6 ± 5.7% of control). Furthermore, the uptake was significantly inhibited in the presence of 100 μM acetyl-L-carnitine, 100 μM gamma-butyrobetaine or 500 μM quinidine. In RT-PCR analysis, the high-affinity carnitine transporter OCTN2 was detected in rat whole testis tissue and primary-cultured Sertoli cells. In contrast, the low-affinity carnitine transporter ATB0,+was detected in rat whole testis tissue, but not in primary cultured Sertoli cells. These results demonstrate that OCTN2 mediates carnitine supply to Sertoli cells from the circulation.

Biodegradation of endocrine disrupting dibutyl phthalate by a bacterial consortium expressing Sphingobium sp. SM42 esterase

2016 ◽  
Vol 51 (8) ◽  
pp. 1040-1045 ◽  
Author(s):  
Pareenart Sungkeeree ◽  
Wirongrong Whangsuk ◽  
James Dubbs ◽  
Skorn Mongkolsuk ◽  
Suvit Loprasert
Keyword(s):  
Dibutyl Phthalate ◽  
Bacterial Consortium ◽  
Endocrine Disrupting
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