Development of an Online-PFSPE-Spectrophotometry System for the Quantification of Environmental Pollutants

In order to detect the environmental pollutants in water sample with high sensitivity, an automatic method based on a combined preconcentration setup and flow injection system was developed. A novel packed fiber solid phase extraction (PFSPE) cartridge packed with electrospun nanofiber was first designed to adsorb environmental pollutants from water media in an online manner. Rhodamine B (RB) was selected as a model pollutant. Under the optimized conditions, a good linearity of wide range between 25-1000 ng mL-1 was achieved. The LOD was 7.5 ng mL-1. The method was applied to analysis RB in water samples with satisfactory results.

Download Full-text

Gold–Carbon Nanocomposites for Environmental Contaminant Sensing

Micromachines ◽

10.3390/mi12060719 ◽

2021 ◽

Vol 12 (6) ◽

pp. 719

Author(s):

Shahrooz Rahmati ◽

William Doherty ◽

Arman Amani Babadi ◽

Muhamad Syamim Akmal Che Mansor ◽

Nurhidayatullaili Muhd Julkapli ◽

...

Keyword(s):

Environmental Pollutants ◽

High Sensitivity ◽

Environmental Crisis ◽

World Population ◽

Chemical Contaminants ◽

Carbon Nanocomposites ◽

Minimal Sample ◽

Wide Range ◽

And Control ◽

Analytical Tools

The environmental crisis, due to the rapid growth of the world population and globalisation, is a serious concern of this century. Nanoscience and nanotechnology play an important role in addressing a wide range of environmental issues with innovative and successful solutions. Identification and control of emerging chemical contaminants have received substantial interest in recent years. As a result, there is a need for reliable and rapid analytical tools capable of performing sample analysis with high sensitivity, broad selectivity, desired stability, and minimal sample handling for the detection, degradation, and removal of hazardous contaminants. In this review, various gold–carbon nanocomposites-based sensors/biosensors that have been developed thus far are explored. The electrochemical platforms, synthesis, diverse applications, and effective monitoring of environmental pollutants are investigated comparatively.

Download Full-text

Immunocytochemical identification of meningeal leukemia and lymphoma: poly-L-lysine-coated slides permit multimarker analysis even with minute cerebrospinal fluid cell specimens

Blood ◽

10.1182/blood.v73.7.1942.1942 ◽

1989 ◽

Vol 73 (7) ◽

pp. 1942-1950 ◽

Cited By ~ 32

Author(s):

BR Kranz ◽

E Thiel ◽

S Thierfelder

Keyword(s):

Cerebrospinal Fluid ◽

Cell Attachment ◽

High Sensitivity ◽

Cell Loss ◽

Accurate Identification ◽

Malignant Lymphoid Cell ◽

Wide Range ◽

Electrostatic Binding ◽

Single Marker ◽

Optimized Conditions

Abstract Use of immunocytology for accurate identification of malignant cells in cerebrospinal fluid (CSF) has so far been hampered by high cell requirements of the immunologic methods hitherto used. In an attempt to minimize cell loss in cytopreparation, electrostatic binding of cells to poly-L-lysine (PLL)-coated multispot slides, followed by immunocytochemistry, was investigated. Using optimized conditions of cell attachment and fixation and performing all washing procedures on the slide made multimarker analysis possible even in paucicellular specimens, while preserving excellent cell morphology and yielding high sensitivity in the detection of antigens. In a study of 26 CSF specimens with inconclusive cytomorphology, comprising 335 single marker determinations, we were able to discriminate reliably between resting or activated benign cells and a wide range of types of malignant lymphoid cell. A definitive diagnosis was reached in all cases by one tap only. Malignant meningitis was ruled out in ten specimens and proved in 16, including five in which the type of malignancy could only be determined by immunophenotyping. We conclude that immunocytochemistry on PLL-coated slides constitutes the method of choice for immunologic cell differentiation in CSF, which allows equivocal morphologic findings to be clarified.

Download Full-text

Liposome-based flow-injection immunoassay for determining theophylline in serum

Clinical Chemistry ◽

10.1093/clinchem/39.3.386 ◽

1993 ◽

Vol 39 (3) ◽

pp. 386-391 ◽

Cited By ~ 33

Author(s):

L Locascio-Brown ◽

A L Plant ◽

R Chesler ◽

M Kroll ◽

M Ruddel ◽

...

Keyword(s):

Flow Injection ◽

Solid Phase ◽

Injection System ◽

Antibody Activity ◽

Excellent Correlation ◽

Serum Theophylline ◽

Solid Phase Reactor ◽

Sequential Samples ◽

Flow Injection System ◽

Comparison Of The Results

Abstract We developed a method for quantitatively determining theophylline in serum, using a heterogeneous immunoassay called flow-injection immunoanalysis. The reaction involves competition between serum theophylline and theophylline-labeled liposomes. Separation occurs on a solid-phase reactor column containing immobilized antibody to theophylline incorporated in a flow-injection system. Subsequent lysis of the bound liposomes provides sensitive detection of the analyte. Effective regeneration of the immobilized antibody activity allows the reactor to be reused for hundreds of sequential samples. Comparison of the results of the flow-injection immunoassay method with results obtained with a commercially available fluorescence polarization method showed an excellent correlation.

Download Full-text