Antioxidant Activity and Chemical Constituents of Microalgae Oil of Schizochytrium aggregatum

2014 ◽  
Vol 919-921 ◽  
pp. 2022-2029 ◽  
Author(s):  
Jun Wei Lv ◽  
Xian Qing Yang ◽  
Lai Hao Li
Keyword(s):  
Antioxidant Activity ◽  
Chemical Constituents ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Absorption Capacity ◽  
Microalgae Oil ◽  
Dpph Radical Scavenging ◽  
Β Carotene ◽  
Radical Scavenging Assay

Abtrast: This research aimed to explore the antioxidant activity of microalgae oil through in vitro experiment and investigate the constituents of microalgae oil. The antioxidant activity was determined using the 1,1-diphenyl-2-picrylhydrazyl(DPPH)radical scavenging assay,2,2-Azinobis (3-ehtylbenzothiazolin-6-sulfnicAcid)(ABTS)radical scavenging assay, the reducing power,β-Carotene bleaching assay and Oxygen Radical Absorption Capacity(ORAC)assay. BHT、α-tocopherol or Vc were compared as the reference. It can be confirmed that microalgae oil has moderate antioxidant ability. According to GC-MS analysis, the most abundant compounds in microalgae oil were palmitic acid, docosahexaenoic acid and tetradecanoic,constituting 38.1%, 34.24% and 3.39%, respectively.

scholarly journals GC-MS Analysis of the Antioxidant Active Fractions of Micromeria juliana with Anticholinesterase Activity

2009 ◽  
Vol 4 (9) ◽  
pp. 1934578X0900400 ◽  
Author(s):  
Mehmet Öztürk ◽  
Ufuk Kolak ◽  
Mehmet Emin Duru ◽  
Mansur Harmandar
Keyword(s):  
Lipid Peroxidation ◽  
Antioxidant Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Light Petroleum ◽  
Inhibition Activity ◽  
Total Antioxidant ◽  
Lipid Peroxidation Inhibition ◽  
Β Carotene

The aerial parts of Micromeria juliana (L.) Bentham ex Reichb. were extracted with light petroleum, acetone and methanol, successively. The antioxidant activity of different concentrations of the extracts was evaluated using different antioxidant tests, namely total antioxidant (lipid peroxidation inhibition activity), DPPH (1,1-diphenyl-2-picrylhydrazyl) radical scavenging, ferric reducing power, and metal chelating. Total antioxidant activity was determined using the β-carotene-linoleic acid assay. Unexpectedly, the light petroleum extract exhibited strong lipid peroxidation inhibition activity. The extract was fractionated on a silica gel column and the antioxidant activity of the fractions was determined by the β-carotene-linoleic assay at 25 μg/mL concentration. The fractions that exhibited more than 50% inhibition activity were analysed by GC and GC/MS; thus, the structure of fourteen compounds were elucidated. In addition, acetyl- and butyrylcholinesterase inhibitory activities of the extracts were also determined in vitro. The light petroleum and acetone extracts were found to have mild butyrylcholinesterase inhibitory activity.

scholarly journals Total Phenolic Contents, DPPH Radical Scavenging and β-Carotene Bleaching Activities of Aqueous Extract from Ammoides atlantica

2020 ◽  
Vol 10 (3-s) ◽  
pp. 196-198
Author(s):  
Karima Loucif ◽  
Hassiba Benabdallah ◽  
Fatima Benchikh ◽  
Soulaf Mehlous ◽  
Chawki Ben Souici ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Medicinal Plants ◽  
Aqueous Extract ◽  
Radical Scavenging ◽  
Health Benefit ◽  
Total Phenolic ◽  
Dpph Radical ◽  
Dpph Radical Scavenging ◽  
Β Carotene

Phytotherapy has known a great evolution all the world and some medicinal plants are important remedies of some diseases. Ammoides atlantica is one of the medicinal plants used in folk medicine. This study aims to estimate the total phenolics and flavonoids contents then to investigate both in vitro antioxidant activity models of aqueous extract (AqE) from Ammoides atlantica. Total polyphenol contents were determined using Folin Ciocalteu's reagent; flavonoids were quantified employing the AlCl3 Method. The in vitro antioxidant property was assessed by DPPH-scavenging radicals and lipid peroxidation assays. The results revealed that Ammoides atlantica aqueous extract presented a high total phenolic and flavonoid contents with values of 85.56±4.71 µg GAE (gallic acid equivalent)/mg and 40.55±4.09 µg QE (quercitin equivalent)/mg dry extract, respectively. This extract shows a good DPPH radical scavenging and β-carotene bleaching activities with an IC50 of 107.48±5.9 µg/mL and 130.17±5.52 µg/mL, respectively. This study indicates that the aqueous extract from Ammoides atlantica has potent antioxidant effects and may prove to be of latent health benefit as well as supplementary sources for natural antioxidants drugs. Keywords: Ammoides atlantica, aqueous extract, antioxidant activity, phenolic compounds.

scholarly journals Ethanolic Extract of Glycyrrhiza glabra to Ameliorate Oxidative Stress – Studies in vitro

2020 ◽  
Vol 2 (1) ◽  
pp. 80
Keyword(s):  
Oxidative Stress ◽  
Antioxidant Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Ethanolic Extract ◽  
Glycyrrhiza Glabra ◽  
New Drugs ◽  
Phytochemical Analysis ◽  
Radical Scavenging Assay

Plants are major sources of bioactive organic molecules that are of interest to the pharmaceutical industry and are being screened for new drugs and chemicals. Glycyrrhiza glabra Linn. (Family: Fabaceae) also known as Liquorice, Mulaithi, or Yashtimadu is a well-known medicinal plant used in traditional medicine. Its roots and rhizomes are the medicinal parts used and are reported to possess antitumor, antimicrobial, antiviral, anti-inflammatory, immunoregulatory activities. This plant is also used as a flavoring agent due to its sweetness. In the present work, the ethanolic extract of Glycyrrhiza glabra was prepared, and its phytochemical analysis was done using HPLC. The in vitro antioxidant assays such as DPPH radical scavenging assay, Hydroxyl radical scavenging assay, total antioxidant activity assay, and total reducing power assay were done, and the results showed significant antioxidant activity of the extract. The extract was analyzed further to evaluate the ability to protect against oxidative stress in chicken liver tissue. The levels of glutathione and lipid peroxidation in H2O2 and/or G glabra extract-treated tissue indicated the potential to protect against oxidative stress under in vitro conditions. The in vitro comet assay results showed that the G glabra extract protected against H2O2 induced cellular DNA damage. These findings indicated promising antioxidant and antigenotoxic potential of G.glabra and need further exploration for translating these findings to its possible health benefits.

Antioxidative and Hepatoprotective Effects of Rubus canescens DC. growing wild in Lebanon

2019 ◽  
Vol 09 ◽  
Author(s):  
Ghaydaa Mohamad Salim ◽  
Mohammad H. El-Dakdouki ◽  
Hiba Abdallah ◽  
Hatem M. Nasser ◽  
Nelly Arnold-Apostolides
Keyword(s):  
Antioxidant Activity ◽  
Histological Analysis ◽  
Radical Scavenging ◽  
Dpph Radical ◽  
Methanolic Extracts ◽  
Phytochemical Profile ◽  
Dpph Radical Scavenging ◽  
Β Carotene

Background: Plants of the genus Rubus (family Rosaceae) have been used for diverse medicinal purposes for centuries. We hypothesized that the scarcely investigated R. canescens DC. should, like other species of the genus Rubus, exhibit prominent antioxidant activity. Objective: The present study investigates the in vitro and in vivo antioxidant activity of fruit juice as well as aqueous and methanolic extracts of the areal parts of Rubus canescens DC., and decipher the phytochemical profile of the methanolic extracts by GC-MS. Methods: The in vitro antioxidant activity was assessed by DPPH radical scavenging assay and β-carotene bleaching assays. The in vivo antioxidant activity of the extracts was assessed by measuring ALT, AST, CAT, and SOD levels in CCl4-challenged mice in two experimental models (chronic exposure and preventive). Histological analysis was conducted on H&E stained liver sections, and the phytochemical profile of methanolic extracts was investigated by GC-MS. Results: DPPH radical scavenging assay revealed that the methanolic leaves extract exhibited the highest activity, while the juice was the most active in the β-carotene bleaching assay. The in vivo experiments suggested that the extracts have promising antioxidant potential and hepatoprotective effect capable of promoting liver functions. Histological analysis of liver sections revealed that administrating juice extract regenerated hepatocytes while reducing inflammation. GC-MS analysis indicated the presence of squalene, β-amyrin, and γ-sitosterol that may have contributed to the observed activity. Conclusion: The current study provided the first in vivo evidence supporting the antioxidative and hepatoprotective effects of R. canescens DC. growing wild in Lebanon.

scholarly journals Ethanolic Extract of Glycyrrhiza glabra to Ameliorate Oxidative Stress – Studies In vitro

2020 ◽  
Vol 10 (2) ◽  
pp. 2289-2297
Keyword(s):  
Oxidative Stress ◽  
Antioxidant Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Ethanolic Extract ◽  
Glycyrrhiza Glabra ◽  
New Drugs ◽  
Phytochemical Analysis ◽  
Radical Scavenging Assay

Plants are major sources of bioactive organic molecules of interest for the pharmaceutical industry and are being screened for new drugs and chemicals. Glycyrrhiza glabra Linn. (Family: Fabaceae) is a very well-known medicinal plant traditionally used as medicine. Its roots and rhizomes are the medicinal parts used and are reported to possess antitumor, antimicrobial, antiviral, anti-inflammatory, immunoregulatory activities. The ethanolic extract of Glycyrrhiza glabra was prepared in the present work, and its phytochemical analysis and HPLC were done. The in vitro antioxidant activity assays such as DPPH radical scavenging assay, Hydroxyl radical scavenging assay, total antioxidant activity assay, and total reducing power assay was done, and the results showed significant antioxidant activity. The extract was analyzed further to evaluate the ability to protect against oxidative stress in chicken liver tissue. The extent of lipid peroxidation and glutathione in H2O2 and/or Glycyrrhiza glabra extract-treated tissue indicated the extract's potential to protect against oxidative stress under in vitro conditions. The in vitro comet assay results showed that the Glycyrrhiza glabra extract protected against H2O2 induced cellular DNA damage. These findings indicated promising antioxidant and antigenotoxic potential of Glycyrrhiza glabra and need further exploration for translating these findings to its possible health benefits.

Curcumin-based antioxidant and glycohydrolase inhibitor compounds: Synthesis and in vitro appraisal of the dual activity against diabetes

2020 ◽  
Vol 16 ◽  
Author(s):  
Sajjad Esmaeili ◽  
Nazanin Ghobadi ◽  
Donya Nazari ◽  
Alireza Pourhossein ◽  
Hassan Rasouli ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Enzyme Inhibitor ◽  
Radical Scavenging Activity ◽  
Curcuma Longa ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Inhibitory Effect ◽  
Curcumin Derivatives ◽  
Reducing Power Assay

Background: Curcumin, as the substantial constituent of the turmeric plant (Curcuma longa), plays a significant role in the prevention of various diseases, including diabetes. It possesses ideal structure features as enzyme inhibitor, including a flexible backbone, hydrophobic nature, and several available hydrogen bond (H-bond) donors and acceptors. Objective: The present study aimed at synthesizing several novel curcumin derivatives and further evaluation of these compounds for possible antioxidant and anti-diabetic properties along with inhibitory effect against two carbohydrate-hydrolyzing enzymes, α-amylase and α-glucosidase, as these enzymes are therapeutic targets for attenuation of postprandial hyperglycemia. Methods: Therefore, curcumin-based pyrido[2,3-d]pyrimidine derivatives were synthesized and identified using an instrumental technique like NMR spectroscopy and then screened for antioxidant and enzyme inhibitory potential. Total antioxidant activity, reducing power assay and 1,1-diphenyl-2-picrylhydrazyl (DPPH• ) radical scavenging activity were done to appraisal the antioxidant potential of these compounds in vitro. Results: Compounds L6-L9 showed higher antioxidant activity while L4, L9, L12 and especially L8 exhibited the best selectivity index (lowest α-amylase/α-glucosidase inhibition ratio). Conclusion: These antioxidant inhibitors may be potential anti-diabetic drugs, not only to reduce glycemic index but also to limit the activity of the major reactive oxygen species (ROS) producing pathways.

scholarly journals Chemical Composition, In Vitro and In Silico Antioxidant Potential of Melissa officinalis subsp. officinalis Essential Oil

Antioxidants ◽  
2021 ◽  
Vol 10 (7) ◽  
pp. 1081
Author(s):  
Matilda Rădulescu ◽  
Călin Jianu ◽  
Alexandra Teodora Lukinich-Gruia ◽  
Marius Mioc ◽  
Alexandra Mioc ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Chemical Composition ◽  
Essential Oil ◽  
In Silico ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Inhibitory Effect ◽  
Melissa Officinalis ◽  
Β Carotene

The investigation aimed to study the in vitro and in silico antioxidant properties of Melissa officinalis subsp. officinalis essential oil (MOEO). The chemical composition of MOEO was determined using GC–MS analysis. Among 36 compounds identified in MOEO, the main were beta-cubebene (27.66%), beta-caryophyllene (27.41%), alpha-cadinene (4.72%), caryophyllene oxide (4.09%), and alpha-cadinol (4.07%), respectively. In vitro antioxidant properties of MOEO have been studied in 2,2’-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) and 2,2-diphenyl-1-picrylhydrazyl (DPPH) free-radical scavenging, and inhibition of β-carotene bleaching assays. The half-maximal inhibitory concentration (IC50) for the radical scavenging abilities of ABTS and DPPH were 1.225 ± 0.011 μg/mL and 14.015 ± 0.027 μg/mL, respectively, demonstrating good antioxidant activity. Moreover, MOEO exhibited a strong inhibitory effect (94.031 ± 0.082%) in the β-carotene bleaching assay by neutralizing hydroperoxides, responsible for the oxidation of highly unsaturated β-carotene. Furthermore, molecular docking showed that the MOEO components could exert an in vitro antioxidant activity through xanthine oxidoreductase inhibition. The most active structures are minor MOEO components (approximately 6%), among which the highest affinity for the target protein belongs to carvacrol.

scholarly journals Antiglycation and Antioxidant Properties of Ficus deltoidea Varieties

2020 ◽  
Vol 2020 ◽  
pp. 1-16
Author(s):  
Nur Sumirah Mohd Dom ◽  
Nurshieren Yahaya ◽  
Zainah Adam ◽  
Nik Mohd Afizan Nik Abd. Rahman ◽  
Muhajir Hamid
Keyword(s):  
Antioxidant Activity ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Protein Carbonyl ◽  
Scavenging Activity ◽  
Dpph Radical ◽  
Dpph Radical Scavenging Activity ◽  
Ficus Deltoidea ◽  
Dpph Radical Scavenging

The present study aimed to evaluate the potential of standardized methanolic extracts from seven Ficus deltoidea varieties in inhibiting the formation of AGEs, protein oxidation, and their antioxidant effects. The antiglycation activity was analyzed based on the inhibition of AGEs, fructosamine, and thiol groups level followed by the inhibition of protein carbonyl formation. The antioxidant activity (DPPH radical scavenging activity and reducing power assay) and total phenolic contents were evaluated. After 28 days of induction, all varieties of Ficus deltoidea extracts significantly restrained the formation of fluorescence AGEs by 4.55–5.14 fold. The extracts also reduced the fructosamine levels by 47.0–86.5%, increased the thiol group levels by 64.3–83.7%, and inhibited the formation of protein carbonyl by 1.36–1.76 fold. DPPH radical scavenging activity showed an IC50 value of 66.81–288.04 μg/ml and reducing power activity depicted at 0.02–0.24 μg/ml. The extent of phenolic compounds present in the extracts ranged from 70.90 to 299.78 mg·GAE/g. Apart from that, correlation studies between the activities were observed. This study revealed that seven varieties of Ficus deltoidea have the potential to inhibit AGEs formation and possess antioxidant activity that might be attributed to the presence of phenolic compounds.

In Vitro Antioxidant Activity Metaplexis Japonica Makino Extract and Derivatives

2014 ◽  
Vol 955-959 ◽  
pp. 387-389 ◽  
Author(s):  
Bao Qing Wang
Keyword(s):  
Antioxidant Activity ◽  
Linoleic Acid ◽  
Ethyl Acetate ◽  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Acid Test ◽  
Acetone Extracts ◽  
Β Carotene ◽  
Ethyl Acetate Extracts

Antioxidant activities of acetone and ethyl acetate extracts from Metaplexis japonica Makino, one of famous medicine plants in the eastnorth region of China, named luomo in Chinese, were examined by a DPPH (1,1-Diphenyl-2-picrylhydrazyl) radical-scavenging assay and a β-carotene-linoleic acid test. In DPPH, the antioxidant activity of the acetone extracts, ethyl acetate extracts and derivative were IC50 were 313.21, 266.92 and 118.78μg/mL, respectively. In the β-carotene-linoleic acid test, IC50 were 285.09, 351.57 and 123.89μg/mL. It was concluded that Metaplexis japonica Makino and its derivatives might be a potential natural source of antioxidants .

ASSESSMENT OF PHYTOCHEMICALS, TOTAL PHENOL, FLAVONOID CONTENT AND IN VITRO ANTIOXIDANT PROPERTY OF LARGE CARDAMOM EXTRACTS

INDIAN DRUGS ◽  
2021 ◽  
Vol 58 (10) ◽  
pp. 34-41
Author(s):  
Kanthlal S. K. ◽  
Jipnomon Joseph ◽  
Bindhu P. Paul ◽  
Vijayakumar M ◽  
Rema Shree A. B. ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Antioxidant Property ◽  
Bioactive Constituents ◽  
Preliminary Assessment ◽  
Reducing Power Assay ◽  
Large Cardamom

Amomum subulatum, commonly known as large or black cardamom, is a commonly used spice in Indian kitchens and is traditionally used to treat various ailments. To add more knowledge about the medicinal values of the fruit, this study was conducted to evaluate the in vitro antioxidant activities of aqueous, methanol, ethanol, hydro alcohol, ethyl acetate, acetone and chloroform extracts of the fruit. Preliminary assessment was done to detect the presence of phytoconstituents using identification tests. The antioxidant activity was measured by employing methods such as diphenylpicrylhydrazyl (DPPH) radical scavenging assay, total antioxidant activity equivalent to ascorbic acid, reducing power assay and superoxide anion scavenging assay. The antioxidant activities were compared with their respective phenol and flavonoid contents. Preliminary assessment revealed that large cardamom fruit is a good source of all the bioactive constituents as well as phenol and flavonoid essential for medicinal values. The extract obtained by polar solvents showed the highest antioxidant efficacy in relation to its phenol content. Also, all the solvent-soluble fractions showed a concentration-dependent antioxidant effect. Results from our study prove that large cardamom can alleviate oxidative stress, suggesting the potential of large cardamom as a functional food

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