Evaluation of Mixed Cellulase-Amylase System on Enzymatic Hydrolysis Reaction Using Response Surface Methodology

2014 ◽  
Vol 974 ◽  
pp. 241-246
Author(s):  
Fazlena Hamzah ◽  
Nurul Husna Saleh ◽  
Nuratiqah Alimin ◽  
Mohamad Sufian So’aib
Keyword(s):  
Enzymatic Hydrolysis ◽  
Substrate Concentration ◽  
Complex Structure ◽  
Glucose Production ◽  
Polymeric Membrane ◽  
Hydrolysis Process ◽  
Effects Of Ph ◽  
Enzyme Dosage ◽  
Face Centered ◽  
Composite Face

The ability of mixed enzyme (ɑ-amylase and cellulase) immobilized via cross-linking method with 3% of glutaraldehyde (GA) on polymeric membrane containing 20 wt% of polysulphone and 80 wt% of 1-methyl-2-pyrrolidone (NMP)/polyvinylpyrrolidone (PVP) to catalyze the complex structure (starch and cellulose) into glucose was investigated in this study. Central Composite Face Centered Design (CCFD) with alpha equal to 1 (ɑ=1) were used to evaluate the effects of pH, temperature, percent of enzyme dosage and percent of substrate concentration on enzymatic hydrolysis process. Maximum glucose concentration (g/L) was obtained at 50°C, pH 6.5, 3% of enzyme dosage and 3% of substrate concentration. The study indicated that only substrate and enzyme ratio play significant role (P < 0.1) in maximizing glucose production within pH, temperature, substrate and enzyme dosage range of pH5-8, 30-70°C, 1-5w/v%, and 1-5w/v% respectively. Kinetic enzyme using Lineweaver-Burk plotting suggested that Km and Vmax for the mixed enzyme in this process were 9.6 g/L and 0.3571 U/mg respectively.

scholarly journals Accumulation of dry substances in hydrolysate during the processing of barley malt sprouts with a celluloid enzyme complex

2021 ◽  
Vol 32 ◽  
pp. 03005
Author(s):  
O. N. Vetrova ◽  
O. Y. Eremina ◽  
N. V. Seregina ◽  
N. V. Shuldeshova
Keyword(s):  
Enzymatic Hydrolysis ◽  
Substrate Concentration ◽  
Enzyme Preparation ◽  
Mutual Influence ◽  
Matter Content ◽  
Cellulolytic Enzymes ◽  
Dry Matter Content ◽  
Barley Malt ◽  
Hydrolysis Process ◽  
Parameter Values

The article presents the results of a study of the enzymatic hydrolysis of barley malt sprouts by cellulolytic enzymes. The influence of the process parameters (temperature, substrate concentration, concentration of the enzyme preparation, duration of the process) on the output of dry substances in the hydrolysate is shown. The results of the experiments were presented by a mathematical model of the process and graphically-in the form of surface projections reflecting the mutual influence of each possible pair of factors on the dry matter content in the hydrolysate. It is found that all the studied factors have an effect on the accumulation of dry substances in the hydrolysate. As a result of optimization the parameters of the enzymatic hydrolysis process, the following parameter values were obtained: temperature - 50 ˚С, duration of enzymatic hydrolysis-89 min, substrate concentration - 0.37 (hydromodule 1:10), concentration of the enzyme preparation - 0.05 %.

Modeling and Kinetic Parameter Estimation of the Enzymatic Hydrolysis Process of Lignocellulosic Materials for Glucose Production

2020 ◽  
Vol 59 (38) ◽  
pp. 16851-16867
Author(s):  
David Sebastián Jiménez-Villota ◽  
Juan Camilo Acosta-Pavas ◽  
Kelly Johana Betancur-Ramírez ◽  
Angela Adriana Ruiz-Colorado
Keyword(s):  
Parameter Estimation ◽  
Enzymatic Hydrolysis ◽  
Kinetic Parameter ◽  
Glucose Production ◽  
Lignocellulosic Materials ◽  
Kinetic Parameter Estimation ◽  
Hydrolysis Process

scholarly journals Enzymatic hydrolysis of extruded soybean meal at high substrate concentrations

2016 ◽  
pp. 63-73
Author(s):  
Anton Sharikov ◽  
Anna Sereda ◽  
Elena Kostyleva ◽  
Irina Velikoretskaya ◽  
Victor Polyakov
Keyword(s):  
Enzymatic Hydrolysis ◽  
Soybean Meal ◽  
Substrate Concentration ◽  
Degree Of Hydrolysis ◽  
High Substrate ◽  
Bacterial Protease ◽  
Biotechnological Processes ◽  
Enzyme Dosage ◽  
Hydrolysis Of ◽  
Substrate Concentrations

Extrusion as a pretreatment before enzymatic hydrolysis of soybean meal is an effective technique to eliminate antinutritional properties of the main thermostable soy proteins glycinin and ?-conglycinin for production of feed ingredients with enhanced properties. In terms of economic efficiency, biotechnological processes are preferable to carry out at high substrate concentrations. The aim of the investigation was to evaluate the influence of high substrate concentrations in the range of 26-32% and enzyme dosages (0.4 - 3.1 PU/g) on efficiency of hydrolysis of extruded toasted soybean meal with bacterial protease. The results showed that maximum degree of hydrolysis was 42.1% at the enzyme dosage of 3.6 PU/g and at the substrate concentration of 29.0%. The increase in the substrate concentration had a strong effect on the deterioration of dynamic viscosity of the hydrolysates from 0.2 to 5.82 Pa?s. A combination of extrusion cooking at 120?C and enzymatic treatment with ?Protolad B? protease enabled hydrolysis of glycinin and ?-conglycinin to peptides with molecular mass below 15 kDa.

Kinetics of Enzymatic Hydrolysis of Cellulose Using Aspergillus niger

2015 ◽  
Vol 1101 ◽  
pp. 294-298
Author(s):  
Akida Mulyaningtyas ◽  
Ratna Ningsih ◽  
Siti Syamsiah ◽  
Sarto ◽  
Wahyudi Budi Sediawan
Keyword(s):  
Enzymatic Hydrolysis ◽  
Glucose Production ◽  
Mass Reduction ◽  
Monod Equation ◽  
Enzymatic Hydrolysis Of Cellulose ◽  
Hydrolysis Process ◽  
Hydrolysis Of Cellulose ◽  
The Relationship ◽  
Kinetics Of ◽  
Hydrolysis Of

This study aimed to analyze the kinetics of enzymatic hydrolysis of cellulose. Cellulase enzyme was obtained fromAspergillusnigergrown on filter paper and the hydrolysis process was carried out in solid state. The kinetic reviewed here was the relationship between glucose production and cellulose mass reduction to optimize the parameters of Monod equation at various moisture levels.

Enzymatic textile recycling – best practices and outlook

2021 ◽  
pp. 0734242X2110291
Author(s):  
Benjamin Piribauer ◽  
Andreas Bartl ◽  
Wolfgang Ipsmiller
Keyword(s):  
Enzymatic Hydrolysis ◽  
Complete Removal ◽  
The European Union ◽  
Textile Processing ◽  
Current State ◽  
Industrial Implementation ◽  
Hydrolysis Process ◽  
Textile Recycling ◽  
Textile Sector ◽  
Suitable Process

Recently, textiles and their end-of-life management have become the focus of public and political attention. In the European Union the revised waste framework directive defines textiles as municipal waste and stipulates their separate collection by 2025. In the context of these developments, this paper summarises briefly the current state-of-the-art in textile recycling. It is evident that recycling methods are not yet fully developed. This is especially the case with multi-material textiles, which are composed of two or more polymers that are incompatible for recycling. In the practical part of the communication, results are presented which show that enzymatic hydrolysis is a suitable process for recycling textiles made of cotton and polyester. After a complete removal of cotton, the remaining pure polyester fibres undergo a re-granulation and post-condensation step. The so obtained recycled polyester is fed back into the textile processing chain and finally towels are obtained. The main steering parameters of the enzymatic hydrolysis process are described. The study proves that solutions in accordance with the Circular Economy in the textile sector are available but an industrial implementation has not yet been realised.

Research on Enzymatic Hydrolysis of Whey Protein

2013 ◽  
Vol 411-414 ◽  
pp. 3205-3209
Author(s):  
Fang Qian ◽  
Lei Zhao ◽  
Shu Juan Jiang ◽  
Guang Qing Mu
Keyword(s):  
Enzymatic Hydrolysis ◽  
Whey Protein ◽  
Degree Of Hydrolysis ◽  
Quadratic Regression ◽  
Orthogonal Regression ◽  
Single Factor Analysis ◽  
Verification Test ◽  
Enzyme Dosage ◽  
Hydrolysis Conditions ◽  
Hydrolysis Of

Based on single factor analysis for the enzymatic hydrolysis of whey protein, papain was selected as the optimal enzyme and its enzymatic hydrolysis conditions were optimized by the quadratic regression orthogonal rotary test. The orthogonal regression model for degree of hydrolysis (DH) to three factors including temperature (X1), time (X2), enzyme dosage (X3) was established as follow: DH=10.40+0.22X1+0.30X2+1.31X3+0.019X1X2+0.011X1X3-0.039X2X3-0.39X12-0.16X22-0.40X32, Verification test showed a DH of 11.7% was obtained at the optimal hydrolysis condition of 56.6°C, 113.8 min and enzyme 8213.7 U /g protein, which basically consisted with the model theoretical value.

Optimization of Fermentable Sugar Production from Pineapple Leaf Waste (Ananas comosus [L.] Merr) by Enzymatic Hydrolysis

2021 ◽  
Vol 18 (1) ◽  
pp. 73-80
Author(s):  
Yohanita Restu Widihastuty ◽  
Sutini Sutini ◽  
Aida Nur Ramadhani
Keyword(s):  
Enzymatic Hydrolysis ◽  
Complex Structure ◽  
Reducing Sugar ◽  
Sugar Yield ◽  
Ananas Comosus ◽  
Optimum Operating ◽  
Cellulose Content ◽  
Optimum Operating Condition ◽  
Operating Condition ◽  
Cellulase Enzyme

Pineapple leaf waste is one agricultural waste that has high cellulose content. Pineapple leaf waste's complex structure contains a bundle of packed fiber that makes it hard to remove lignin and hemicellulose structure, so challenging to produce reducing sugar. Dried pineapple leaf waste pretreated with a grinder to break its complex structure. Delignification process using 2% w/v NaOH solution at 87oC for 60 minutes has been carried out to remove lignin and hemicellulose structure so reducing sugar could be produced. Delignified pineapple leaf waste has been enzymatic hydrolyzed using cellulase enzyme (6 mL, 7 mL, and 8 mL) to produce reducing sugar. The sample was incubated in an incubator shaker at 155 rpm at 45, 55, and 60oC for 72 hours. Determination of reducing sugar yield had been carried out using the Dubois method and HPLC. The model indicated that the optimum operating condition of enzymatic hydrolysis is 7 mL of cellulase enzyme at 55oC to produce 96,673 mg/L reducing sugar. This result indicated that the enzymatic hydrolysis operating condition improved the reducing sugar yield from pineapple leaf waste. The optimum reducing sugar yield can produce biofuel by the saccharification process.

scholarly journals Improved Sugar Production by Optimizing Planetary Mill Pretreatment and Enzyme Hydrolysis Process

2015 ◽  
Vol 2015 ◽  
pp. 1-5
Author(s):  
Jeong Heo Kwon ◽  
Siseon Lee ◽  
Jae-Won Lee ◽  
Youn-Woo Hong ◽  
Jeong Ho Chang ◽  
...  
Keyword(s):  
Glucose Production ◽  
Process Variable ◽  
Enzyme Concentration ◽  
Planetary Mill ◽  
Enzyme Hydrolysis ◽  
Experimental Conditions ◽  
Citrate Buffer ◽  
Pinus Rigida ◽  
Hydrolysis Process ◽  
Optimal Values

This paper describes an optimization of planetary mill pretreatment and saccharification processes for improving biosugar production. Pitch pine (Pinus rigida) wood sawdust waste was used as biomass feedstock and the process parameters optimized in this study were the buffering media, the milling time, the enzyme quantity, and the incubation time. Glucose yields were improved when acetate buffer was used rather than citrate buffer. Initially, with each process variable tests, the optimal values were 100 minutes of milling, an enzyme concentration of 16 FPU/g-biomass, and a 12-hour enzymatic hydrolysis. Typically, interactions between these experimental conditions and their effects on glucose production were next investigated using RSM. Glucose yields from thePinus rigidawaste exceeded 80% with several of the conditions tested, demonstrating that milling can be used to obtain high levels of glucose bioconversion from woody biomass for biorefinery purposes.

scholarly journals Enzymatic hydrolysis of oil palm empty fruit bunch to produce reducing sugar and its kinetic Hidrolisis enzimatik tandan kosong kelapa sawit untuk menghasilkan gula pereduksi dan kinetikanya

2016 ◽  
Vol 83 (1) ◽  
Author(s):  
Vera BARLIANTI ◽  
Deliana DAHNUM ◽  
. MURYANTO ◽  
Eka TRIWAHYUNI ◽  
Yosi ARISTIAWAN ◽  
...  
Keyword(s):  
Enzymatic Hydrolysis ◽  
Oil Palm ◽  
Volume Ratio ◽  
Enzyme Concentration ◽  
Reducing Sugar ◽  
Economic Feasibility ◽  
First Order ◽  
Empty Fruit Bunches ◽  
Hydrolysis Process ◽  
Hydrolysis Of

Abstrak Sebagai salah satu Negara penghasil minyak kelapa sawit mentah (CPO), Indonesia juga menghasilkan tandan kosong kelapa sawit (TKKS) dalam jumlah besar. TKKS terdiri dari-tiga-komponen utama, yaitu selulosa, hemiselulosa, dan lignin. Pengolahan awal TKKS secara alkalindi ikuti dengan hidrolisis TKKS secara enzimatik menggunakan kombinasi enzim selulase dan β-glukosidase akan menghasilkan gula-gula yang mudah difermentasi.  Penelitian ini bertujuan untuk mempelajari pengaruh konsentrasi substrat, kon-sentrasi enzim, dan suhu selama proses hidrolisis berlangsung.  Hasil yang diperoleh menunjukkan bahwa konsentrasi gula maksimum (194,78 g/L) dicapai pada konsentrasi TKKS 20% (b/v), konsentrasi campuran enzim yang terdiri dari selulase dan β-1,4 glukosidase sebesar 3,85% (v/v), dan suhu 50oC. Perbandingan antara selulase dan β-1,4 glukosidase adalah 5:1 dengan masing-masing aktivitas enzim sebesar 144.5 FPU/mL dan 63 FPU/mL. Hasil penelitian juga menunjukkan bahwa model kinetika yang sesuai untuk proses hidrolisis TKKS secara enzimatik adalah model kinetika Shen dan Agblevor dengan reakside aktivasi enzim orde satu.  Hasil ini mendukung studi kelayakan ekonomi dalam pemanfaatan TKKS untuk produksi bioetanol.AbstractAs one of the crude palm oil producers, Indonesia also produces empty fruit bunches (EFB)in large quantities. The oil palm EFB consist of cellulose, hemicellulose and lignin. Alkaline pretreatment of EFB, followed by enzymatic hydro-lysis of cellulose using combination of cellulase and β-glucosidase enzymes produce fermentable sugars. This paper reported the effects of substrate loading, enzyme concentration, and temperature of hydrolysis process on reducing sugar production. The  maximum  sugar  concentration (194.78 g/L) was produced at 50oC using 20% (w/v) EFB and 3.85% (v/v) mixed enzymes of cellulase and β-1,4 glucosidase in volume ratio of 5:1 (v/v), with enzyme activity of 144.5 FPU/mL and 63 FPU/mL, respectively. The results also showed that the suitable kinetic model for enzymatic hydrolysis process of oil palm EFB follow Shen and Agblevor model with first order of enzyme deactivation. These results support the economic feasibility study in utilization of EFB of oil palm for bioethanol production.    

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