scholarly journals Accumulation of dry substances in hydrolysate during the processing of barley malt sprouts with a celluloid enzyme complex

2021 ◽  
Vol 32 ◽  
pp. 03005
Author(s):  
O. N. Vetrova ◽  
O. Y. Eremina ◽  
N. V. Seregina ◽  
N. V. Shuldeshova
Keyword(s):  
Enzymatic Hydrolysis ◽  
Substrate Concentration ◽  
Enzyme Preparation ◽  
Mutual Influence ◽  
Matter Content ◽  
Cellulolytic Enzymes ◽  
Dry Matter Content ◽  
Barley Malt ◽  
Hydrolysis Process ◽  
Parameter Values

The article presents the results of a study of the enzymatic hydrolysis of barley malt sprouts by cellulolytic enzymes. The influence of the process parameters (temperature, substrate concentration, concentration of the enzyme preparation, duration of the process) on the output of dry substances in the hydrolysate is shown. The results of the experiments were presented by a mathematical model of the process and graphically-in the form of surface projections reflecting the mutual influence of each possible pair of factors on the dry matter content in the hydrolysate. It is found that all the studied factors have an effect on the accumulation of dry substances in the hydrolysate. As a result of optimization the parameters of the enzymatic hydrolysis process, the following parameter values were obtained: temperature - 50 ˚С, duration of enzymatic hydrolysis-89 min, substrate concentration - 0.37 (hydromodule 1:10), concentration of the enzyme preparation - 0.05 %.

scholarly journals Enzymatic hydrolysis at high dry matter content: The influence of the substrates’ physical properties and of loading strategies on mixing and energetic consumption

2018 ◽  
Vol 250 ◽  
pp. 191-196 ◽  
Author(s):  
Federico Battista ◽  
Mélanie Gomez Almendros ◽  
Romain Rousset ◽  
Serge Boivineau ◽  
Pierre-Antoine Bouillon
Keyword(s):  
Enzymatic Hydrolysis ◽  
Physical Properties ◽  
Dry Matter ◽  
Matter Content ◽  
Dry Matter Content ◽  
High Dry Matter

Biomass processing into ethanol: pretreatment, enzymatic hydrolysis, fermentation, rheology, and mixing

2017 ◽  
Vol 6 (1) ◽  
pp. 1-22 ◽  
Author(s):  
Bohdan Volynets ◽  
Farhad Ein-Mozaffari ◽  
Yaser Dahman
Keyword(s):  
Enzymatic Hydrolysis ◽  
Fermentation Broth ◽  
Matter Content ◽  
Dry Matter Content ◽  
Stirred Tanks ◽  
High Dry Matter ◽  
Biomass Processing ◽  
Renewable Feedstock ◽  
Ethanol Pretreatment ◽  
Key Steps

AbstractAlternate energy resources need to be developed to amend for depleting fossil fuel reserves. Lignocellulosic biomass is a globally available renewable feedstock that contains a rich sugar platform that can be converted into bioethanol through appropriate processing. The key steps of the process, pretreatment, enzymatic hydrolysis, and fermentation, have undergone considerable amount of research and development over the past decades nearing the process to commercialization. In order for the commercialization to be successful, the process needs to be operated at high dry matter content of biomass, especially in the enzymatic hydrolysis stage that influences ethanol concentration in the final fermentation broth. Biomass becomes a thick paste with challenging rheology for mixing to be effective. As the biomass consistency increases, yield stress increases which limits efficiency of mixing with conventional stirred tanks. The purpose of this review is to provide features and perspectives on processing of biomass into ethanol. Emphasis is placed on rheology and mixing of biomass in the enzymatic hydrolysis step as one of the forefront issues in the field.

The effect of harvest time, dry matter content and mechanical pretreatments on anaerobic digestion and enzymatic hydrolysis of miscanthus

2016 ◽  
Vol 218 ◽  
pp. 1008-1015 ◽  
Author(s):  
Susanne Frydendal-Nielsen ◽  
Maibritt Hjorth ◽  
Sanmohan Baby ◽  
Claus Felby ◽  
Uffe Jørgensen ◽  
...  
Keyword(s):  
Anaerobic Digestion ◽  
Enzymatic Hydrolysis ◽  
Dry Matter ◽  
Matter Content ◽  
Dry Matter Content ◽  
Harvest Time ◽  
Hydrolysis Of

scholarly journals Biological Pretreatment of Corn Stover for Enhancing Enzymatic Hydrolysis Using Thermoalkalotolerant Cellulolytic Enzymes Produced by Bacillus sp. P3

2021 ◽  
Author(s):  
Yanwen Wu ◽  
Haipeng Guo ◽  
Md. Shafiqur Rahman ◽  
Xuantong Chen ◽  
Jinchi Zhang ◽  
...  
Keyword(s):  
Enzymatic Hydrolysis ◽  
Corn Stover ◽  
Fermentation Broth ◽  
Removal Rate ◽  
Enzymatic Saccharification ◽  
Cellulolytic Enzymes ◽  
Biological Pretreatment ◽  
Hydrolysis Process ◽  
Hemicellulose Removal ◽  
Hydrolysis Of

Abstract The biological pretreatment for the enzymatic hydrolysis of lignocellulosic biomasses largely depends on an effective pretreatment process. A significant enhancement of enzymatic saccharification was obtained with corn stover using Bacillus sp. P3. The hemicellulose removal from corn stover by the strain Bacillus sp. P3 was evaluated for enhancing subsequent enzymatic hydrolysis. Therefore, our study revealed that an alkaline resistant xylanase produced by Bacillus sp. P3 in fermentation broth led to a substantially enhanced hemicellulose removal rate from corn stover within pH 9.36–9.68. However, after 20 d pretreatment of corn stover by the strain P3, the glucan content was increased by 51% and the xylan content was decreased by 35%. After 72 h of saccharification using 20 U g− 1 of commercial cellulase, the yield of reducing sugar released from 20 d pretreated corn stover was increased by 56% in comparison to the untreated corn stover. Therefore, the use of the strain P3 could be a promising approach to pretreat corn stover for enhancing the enzymatic hydrolysis process of industrial bioenergy productions.

THE FEEDING VALUE POTENTIAL OF POTATO VINES

1978 ◽  
Vol 58 (4) ◽  
pp. 559-569 ◽  
Author(s):  
J. W. G. NICHOLSON ◽  
D. A. YOUNG ◽  
R. E. McQUEEN ◽  
H. DE JONG ◽  
F. A. WOOD
Keyword(s):  
Dry Matter ◽  
Solanum Tuberosum L ◽  
Sampling Period ◽  
Matter Content ◽  
Water Soluble ◽  
Soluble Carbohydrate ◽  
Dry Matter Content ◽  
Barley Malt ◽  
N Content ◽  
Plastic Bag

The potential use of green potato (Solanum tuberosum L.) vines as a livestock feed was investigated. Fresh vines contained less than 12% dry matter (DM) and did not produce a stable silage in plastic bag, laboratory silos. Satisfactory silages were produced by wilting or by the addition of barley, malt, barley + malt, molasses, chopped hay, chopped hay + barley + malt, formaldehyde and/or formic acid. Only the silages from wilted vines or those containing hay had a suitable consistency for storage and handling in conventional silage systems. Sheep readily ate a silage made from potato vines with the addition of 5% barley, 0.5% malted barley and 20% chopped hay or straw. The dry matter content of the vines from two cultivars changed only slightly over a 5-wk sampling period but the acid detergent fiber (ADF) content increased and the N content dropped. Except for the cultivar Wischip, there was little variation among nine cultivars sampled on the same day for pH (5.15–5.95), DM (10.3–12.9%) and ADF (26.5–32.4%). The N content ranged from 1.6 to 2.4% of the DM and the water soluble carbohydrate content from 7.5 to 3.3% of the DM. The levels of glycoalkaloids found should be safe for ruminant animals. It was concluded that potato vines harvested prior to senescence contain a useful level of nutrients for ruminants. Pesticide residues and mechanical systems for harvesting the vines are major concerns to be resolved before on-farm application can be recommended.

Evaluation of Mixed Cellulase-Amylase System on Enzymatic Hydrolysis Reaction Using Response Surface Methodology

2014 ◽  
Vol 974 ◽  
pp. 241-246
Author(s):  
Fazlena Hamzah ◽  
Nurul Husna Saleh ◽  
Nuratiqah Alimin ◽  
Mohamad Sufian So’aib
Keyword(s):  
Enzymatic Hydrolysis ◽  
Substrate Concentration ◽  
Complex Structure ◽  
Glucose Production ◽  
Polymeric Membrane ◽  
Hydrolysis Process ◽  
Effects Of Ph ◽  
Enzyme Dosage ◽  
Face Centered ◽  
Composite Face

The ability of mixed enzyme (ɑ-amylase and cellulase) immobilized via cross-linking method with 3% of glutaraldehyde (GA) on polymeric membrane containing 20 wt% of polysulphone and 80 wt% of 1-methyl-2-pyrrolidone (NMP)/polyvinylpyrrolidone (PVP) to catalyze the complex structure (starch and cellulose) into glucose was investigated in this study. Central Composite Face Centered Design (CCFD) with alpha equal to 1 (ɑ=1) were used to evaluate the effects of pH, temperature, percent of enzyme dosage and percent of substrate concentration on enzymatic hydrolysis process. Maximum glucose concentration (g/L) was obtained at 50°C, pH 6.5, 3% of enzyme dosage and 3% of substrate concentration. The study indicated that only substrate and enzyme ratio play significant role (P < 0.1) in maximizing glucose production within pH, temperature, substrate and enzyme dosage range of pH5-8, 30-70°C, 1-5w/v%, and 1-5w/v% respectively. Kinetic enzyme using Lineweaver-Burk plotting suggested that Km and Vmax for the mixed enzyme in this process were 9.6 g/L and 0.3571 U/mg respectively.

Optimum harvest date of maize for biogas and silage purposes

2009 ◽  
Vol 57 (2) ◽  
pp. 119-125
Author(s):  
G. Hadi
Keyword(s):  
Dry Matter ◽  
Biogas Production ◽  
Weather Conditions ◽  
Vegetation Period ◽  
Dry Mass ◽  
Matter Content ◽  
Harvest Date ◽  
Dry Matter Content ◽  
Dry Matter Yield ◽  
Vegetative Organs

The dry matter and moisture contents of the aboveground vegetative organs and kernels of four maize hybrids were studied in Martonvásár at five harvest dates, with four replications per hybrid. The dry matter yield per hectare of the kernels and other plant organs were investigated in order to obtain data on the optimum date of harvest for the purposes of biogas and silage production.It was found that the dry mass of the aboveground vegetative organs, both individually and in total, did not increase after silking. During the last third of the ripening period, however, a significant reduction in the dry matter content was sometimes observed as a function of the length of the vegetation period. The data suggest that, with the exception of extreme weather conditions or an extremely long vegetation period, the maximum dry matter yield could be expected to range from 22–42%, depending on the vegetation period of the variety. The harvest date should be chosen to give a kernel moisture content of above 35% for biogas production and below 35% for silage production. In this phenophase most varieties mature when the stalks are still green, so it is unlikely that transport costs can be reduced by waiting for the vegetative mass to dry.

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