Development of Aldehyde Hyaluronic Acid - N,O-Carboxymethyl Chitosan based Hydrogel for Intraperitoneal Antiadhesion Application

2021 ◽  
Vol 52 ◽  
pp. 47-54
Author(s):  
Prihartini Widiyanti ◽  
Yolanda Citra Ayu Priskawati ◽  
Herry Wibowo ◽  
Jan Ady
Keyword(s):  
Fourier Transform ◽  
Hyaluronic Acid ◽  
Infrared Spectroscopy ◽  
Degradation Rate ◽  
Synthesis Method ◽  
Carboxymethyl Chitosan ◽  
Gynecological Surgery ◽  
Swelling Ratio ◽  
Optimal Composition ◽  
Aldehyde Derivative

Intraperitoneal adhesion is a serious case that often occurs with a prevalence of 90-97 % after undergoing gynecological surgery and laparotomy. This study aims are to characterized the hydrogel and identified the optimal composition of Hyaluronic acid (HA) - N, O-carboxymethyl chitosan (NOCC) as an anti-adhesion biomaterial barrier. The synthesis method involved firstly the synthesis of aldehyde derivative of hyaluronic acid (AHA) and also the conversion of chitosan into its derivative, N,O-carboxymethyl chitosan. These two compounds were mixed in various compositions and crosslinked to form N, O-carboxymethyl chitosan (NOCC) /AHA. Fourier-transform infrared spectroscopy has confirmed that the functional groups found -C = O stretching at 1644 cm-1 indicating the hyaluronic acid and carboxymethyl group (-CH2COOH) in 1380 cm-1 which indicate the presence of chitosan. The crosslink is evidenced by the group C = N stretching at a wavenumber of about 1630 cm-1. The best composition of intraperitoneal anti-adhesion is the ratio of hyaluronic acid: chitosan at 30:10 mg/ml. The swelling test is showed a swelling ratio of around 211.8 % in accordance with the standard as intraperitoneal anti-adhesion. Hydrogel has a degradation rate up to 86.87 % on day 10, and this is in accordance with the standard as intraperitoneal anti-adhesion. Cytotoxicity assay showed that hydrogel was nontoxic with a percentage of 92.9 % cell viability. The newly developed hyaluronic acid-carboxymethyl chitosan has characteristics that conform to the criteria of an intraperitoneal anti-adhesion.

Preparation and Structure of BiVO4-Graphene Composites

2014 ◽  
Vol 905 ◽  
pp. 101-104 ◽  
Author(s):  
Bin Zhang ◽  
Ya Ling Wang ◽  
Lang Huan Huang ◽  
Shao Zao Tan
Keyword(s):  
Fourier Transform ◽  
Infrared Spectroscopy ◽  
Electron Microscope ◽  
Hydrothermal Synthesis ◽  
Emission Spectroscopy ◽  
Synthesis Method ◽  
Photoelectron Emission ◽  
One Pot ◽  
X Ray ◽  
Transmission Electron

BiVO4-graphene composites were prepared through a one-pot hydrothermal synthesis method, and the structure were investigated by Fourier transform infrared spectroscopy (FTIR), X-ray photoelectron emission spectroscopy (XPS), X-ray powder diffraction (XRD) and Transmission electron microscope (TEM). The results show that BiVO4-graphene composites are successfully prepared, and BiVO4 particles are almost uniformly deposited on the surface of graphene.

Polystyrene Beads Modified with Ester Group and their Application in N-Heptane/Toluene Separation

2014 ◽  
Vol 1048 ◽  
pp. 423-426
Author(s):  
Huan Yang Yu
Keyword(s):  
Thermal Stability ◽  
Fourier Transform ◽  
Infrared Spectroscopy ◽  
Thermogravimetric Analysis ◽  
Fourier Transform Infrared Spectroscopy ◽  
Fourier Transform Infrared ◽  
Ester Group ◽  
Swelling Ratio ◽  
Excellent Thermal Stability ◽  
Polystyrene Beads

Polystyrene beads modified with ester group for the separation of n-heptane/toluene have been successfully prepared. The synthesized beads were characterized by fourier transform infrared spectroscopy and thermogravimetric analysis. The effects of ester group on equillibrium swelling ratio and toluene selectivity were studied. The selective factor firstly increases then decreases with the increasing of ester group content. When the content of ester group is 30%, selective factor reaches maximum, the value is 1.62. Polystyrene beads modified with ester group show excellent thermal stability.

scholarly journals Elucidation of the time-dependent degradation process in insoluble hyaluronic acid formulations with a controlled degradation rate

2019 ◽  
Vol 10 ◽  
pp. 204173141988503 ◽  
Author(s):  
Akiko Uemura ◽  
Shingo Ogawa ◽  
Yasuyuki Isono ◽  
Ryou Tanaka
Keyword(s):  
Hyaluronic Acid ◽  
Degradation Rate ◽  
Morphological Changes ◽  
Sodium Hyaluronate ◽  
Degradation Process ◽  
Pharmaceutical Products ◽  
The Body ◽  
Time Dependent ◽  
Swelling Ratio

Degradation rate of hyaluronic acid to prolong its stability in vivo would be beneficial. We investigated a potential solution for prolonging the stability of hyaluronic acid within the body. We focused on decreasing the swelling ratio to slow the degradation rate of hyaluronic acid by insolubilizing sodium hyaluronate without using potentially harmful substances such as crosslinkers or modifiers. Hyaluronic acid formulations were created with three different swelling ratios and time-dependent morphological changes in hyaluronic acid formulations and were scored based on each swelling ratio. In vivo degradation was modeled in simulated body fluid and the extent of decay of test membranes were monitored over time. Results showed that, by adjusting the swelling ratio, the degradation rate of hyaluronic acid formulation could be controlled. Our research could lead to improvements in many products, not only preventive materials for postoperative adhesions, but also pharmaceutical products such as osteoarthritis treatments and cosmetic medicines.

scholarly journals Screening of the Chemical Composition and Identification of Hyaluronic Acid in Food Supplements by Fractionation and Fourier-Transform Infrared Spectroscopy

Polymers ◽  
2021 ◽  
Vol 13 (22) ◽  
pp. 4002
Author(s):  
Tamilla Mirzayeva ◽  
Jana Čopíková ◽  
František Kvasnička ◽  
Roman Bleha ◽  
Andriy Synytsya
Keyword(s):  
Fourier Transform ◽  
Hyaluronic Acid ◽  
Infrared Spectroscopy ◽  
Chemical Composition ◽  
Fourier Transform Infrared Spectroscopy ◽  
Fourier Transform Infrared ◽  
Food Supplements ◽  
Protective Effects ◽  
Sequential Fractionation ◽  
Vibrational Bands

Hyaluronic acid, together with collagen, vitamins or plant extracts, is a part of many cosmetic and food preparations. For example, this polysaccharide is used in formulation of many food supplements due to its protective effects on human health. In this work, the screening of the chemical composition of three chosen dietary supplements (powder, tablets and capsules) containing hyaluronic acid was carried out using Fourier-transform infrared spectroscopy. Because of the low amount of analyte in all these samples, it was isolated or concentrated prior to the analysis using a suitable sequential fractionation protocol. Individual isolation procedures were established for each sample based on their declared composition. Firstly, the major components such as collagen or vitamins were removed to obtain polysaccharide fractions by the enzymatic treatment and/or washing out with the appropriate solvents. In some cases, the water insoluble part was removed from the rest dissolved in water. Then, hyaluronic acid was precipitated with copper(II) cations and thus separated from the other polysaccharides. Finally, the analyte was identified in the enriched fractions by the characteristic vibrational bands. The amount of hyaluronic acid in the purified fractions was determined in three ways: gravimetrically, spectrophotometrically, and using isotachophoresis. The combination of the appropriate preparative and analytical steps led to the successful evaluation of chemical composition, finding and quantification of hyaluronic acid in all the studied samples.

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