Comparative Study between In Vivo Bone Ingrowth Behavior in Rabbits and In Vitro Apatite Forming Ability in Simulated Body Fluid Using Granules of P2O5-Free Na2O-CaO-SiO2 Glass System

2001 ◽  
Vol 218-220 ◽  
pp. 193-194
Author(s):  
Shunsuke Fujibayashi ◽  
Shigeru Nishiguchi ◽  
Jiro Tamura ◽  
Takashi Nakamura ◽  
Hyun Min Kim ◽  
...  
Keyword(s):  
Simulated Body Fluid ◽  
Comparative Study ◽  
Body Fluid ◽  
Bone Ingrowth ◽  
Glass System

In Vitro and In Vivo Degradation, Mechanical Properties, and Histocompatibility of Weft-Knitted Silk Mesh-Like Grafts

2022 ◽  
Vol 12 (2) ◽  
pp. 411-416
Author(s):  
Liang Tang ◽  
Si-Yu Zhao ◽  
Ya-Dong Yang ◽  
Geng Yang ◽  
Wen-Yuan Zhang ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Simulated Body Fluid ◽  
Body Fluid ◽  
Degradation Rate ◽  
Maximum Load ◽  
Artificial Ligament ◽  
In Vivo Degradation

To investigate the degradation, mechanical properties, and histocompatibility of weft-knitted silk mesh-like grafts, we carried out the In Vitro and In Vivo silk grafts degradation assay. The In Vitro degradation experiment was performed by immersing the silk grafts in simulated body fluid for 1 year, and the results showed that the degradation rate of the silk mesh-like grafts was very slow, and there were few changes in the mechanical properties and quality of the silk mesh-like graft. In Vivo degradation assay was taken by implantation of the silk mesh-like grafts into the subcutaneous muscles of rabbits. At 3, 6, and 12 months postoperation, the rate of mass loss was 19.36%, 31.84%, and 58.77%, respectively, and the maximum load was 63.85%, 34.63%, and 10.76%, respectively of that prior to degradation. The results showed that the degradation rate of the silk graft and the loss of mechanical properties In Vivo were faster than the results obtained in the In Vitro experiments. In addition, there were no significant differences in secretion of serum IL-6 and TNF-α between the experimental and normal rabbits (P >0.05), suggesting no obvious inflammatory reaction. The findings suggest that the weft-knitted silk mesh-like grafts have good mechanical properties, histocompatibility, and In Vivo degradation rate, and therefore represent a candidate material for artificial ligament

Apatite Formation on Porous TCP In Vitro: A Comparative Study between Static and Dynamic RSBF Systems

2007 ◽  
Vol 280-283 ◽  
pp. 1581-1584
Author(s):  
Chun Lin Deng ◽  
Ji Yong Chen ◽  
Yang Leng ◽  
Xin Long Wang ◽  
Yao Wu ◽  
...  
Keyword(s):  
Simulated Body Fluid ◽  
Comparative Study ◽  
Dynamic System ◽  
Tricalcium Phosphate ◽  
Body Fluid ◽  
Apatite Formation ◽  
Static System ◽  
Inner Pore

Porous tricalcium phosphate ceramics were immersed in static and dynamic revised simulated body fluid (RSBF) at 37°C. Morphology, composition and phase of precipitates on TCP were identified by SEM, FTIR and TEM methods. FTIR and TEM results indicated the deposits on the inner pore walls of TCP were OCP, and SEM results implied that the deposited way of precipitates in static system was different from that in dynamic system.

A Study of Bone-Like Apatite Formation on β-TCP/PLLA Scaffold in Static and Dynamic Simulated Body Fluid

2007 ◽  
Vol 330-332 ◽  
pp. 483-486
Author(s):  
Yun Qing Kang ◽  
Guang Fu Yin ◽  
Ke Feng Wang ◽  
Lin Luo ◽  
Li Liao ◽  
...  
Keyword(s):  
Simulated Body Fluid ◽  
Body Fluid ◽  
Composite Scaffold ◽  
Pore Wall ◽  
Apatite Formation ◽  
X Ray Diffraction ◽  
Good Ability ◽  
Ft Ir

The ability of apatite to form on the surface of biomaterials in simulated body fluid (SBF) has been widely used to predict the bone-bonding ability of bioceramic and bioceramic/polymer composites in vivo. Porous β-tricalcium phosphate/poly(L-lactic acid) (β-TCP/PLLA) composite scaffold was synthesized by new method. The ability of inducing calcium phosphate (Ca-P) formation was compared in static simulated body fluid(sSBF) and dynamic simulated body fluid (dSBF). The Ca-P morphology and crystal structures were identified using SEM, X-ray diffraction and Fourier transform infrared (FT-IR) spectroscopy. The results showed that the typical features of bone-like apatite formation on the surface and the inner pore wall of β-TCP/PLLA. Ca-P formation on scaffold surfaces in dSBF occurred slower than in sSBF and was more difficult with increasing flow rate of dSBF. The ability of apatite to form on β-TCP/PLLA was enhanced by effect of each other that has different degradable mechanism. Porous β-TCP/PLLA composite scaffold indicates good ability of Ca-P formation in vitro.

In vitro biodegradability of bacterial cellulose by cellulase in simulated body fluid and compatibility in vivo

Cellulose ◽  
2016 ◽  
Vol 23 (5) ◽  
pp. 3187-3198 ◽  
Author(s):  
Baoxiu Wang ◽  
Xiangguo Lv ◽  
Shiyan Chen ◽  
Zhe Li ◽  
Xiaoxiao Sun ◽  
...  
Keyword(s):  
Simulated Body Fluid ◽  
Bacterial Cellulose ◽  
Body Fluid

Study on Plasma Sprayed Calcium Silicate Coatings

2005 ◽  
Vol 475-479 ◽  
pp. 2371-2374 ◽  
Author(s):  
Xue Bin Zheng ◽  
Xuan Yong Liu ◽  
Wei Chang Xue ◽  
Chuan Xian Ding
Keyword(s):  
Simulated Body Fluid ◽  
Bond Strength ◽  
Body Fluid ◽  
Strength Test ◽  
Dicalcium Silicate ◽  
Cell Culturing ◽  
Ha Coating ◽  
Plasma Sprayed

Wollastonite and dicalcium silicate coatings have been prepared on Ti-6Al-4V substrate via plasma spraying. Bond strength test, simulated body fluid (SBF) immersion, in vitro cell culturing, and in vivo implantation were carried out to evaluate their mechanical and biological characteristics. The results obtained showed that both coatings possess higher bond strength as compared with hydroxyapatite (HA) coating. In the meanwhile, the good bioactivity and biocompatibility were confirmed in this study.

A Comparative Study Between Dynamic and Static Simulated Body Fluid Methods

2006 ◽  
Vol 309-311 ◽  
pp. 271-274 ◽  
Author(s):  
Ji Yong Chen ◽  
You Rong Duan ◽  
Chun Lin Deng ◽  
Qi Yi Zhang ◽  
Xing Dong Zhang
Keyword(s):  
Simulated Body Fluid ◽  
Body Fluid ◽  
Good Method ◽  
Nucleation And Growth ◽  
Ion Concentration ◽  
Osseous Tissue ◽  
Key Factors ◽  
Good Agreement

In vitro method has often been used in the biodegradation/bioactivity evaluation of bioactive ceramics for its convenience and saving in time and outlay. The simulated body fluid (SBF) suggested by Kokubo was a good simulation of the osteoproduction environment in osseous tissue and has been proved to be a good method to study the bioactivity of biomaterials and the mechanism of bone bonding. But SBF is not a suitable method to research the osteoinduction of biomaterials. The results from SBF were not consistent with that from in vivo in muscle. The local ion concentration is the key factors to affect the nucleation and growth of apatite. In muscle the effect of body fluid flowing on local ion concentration cannot be ignored. A dynamic SBF suggested by these authors of this paper not only simulated the ion concentration of body fluid, but also simulated the effect of body fluid flowing on the local ion concentration near the surface or in biomaterials in muscle. The results from the dynamic SBF were in good agreement with that of the implantation experiments in muscle. The results from dynamic SBF showed that apatite only formed on the walls of macropores of the porous CaP, no apatite formed on the surface of both dense and porous CaP. The new bone only formed on the walls of macropores of porous CaP implanted in muscles, no apatite or osseous tissue could be found on the surfaces of both porous and dense CaP. The dynamic SBF preferably simulated the osteoinduction environment in non-osseous tissue and can be used in osteoinductivity evaluation of bioceramics.

scholarly journals The Use of Simulated Body Fluid (SBF) for Assessing Materials Bioactivity in the Context of Tissue Engineering: Review and Challenges

Biomimetics ◽  
2020 ◽  
Vol 5 (4) ◽  
pp. 57 ◽  
Author(s):  
Francesco Baino ◽  
Seiji Yamaguchi
Keyword(s):  
Simulated Body Fluid ◽  
Body Fluid ◽  
Material Surface ◽  
In Vitro Bioactivity ◽  
Testing Methods ◽  
Apatite Coating ◽  
Living Bone ◽  
Glass Compositions

Some special implantable materials are defined as “bioactive” if they can bond to living bone, forming a tight and chemically-stable interface. This property, which is inherent to some glass compositions, or can be induced by applying appropriate surface treatments on otherwise bio-inert metals, can be evaluated in vitro by immersion studies in simulated body fluid (SBF), mimicking the composition of human plasma. As a result, apatite coating may form on the material surface, and the presence of this bone-like “biomimetic skin” is considered predictive of bone-bonding ability in vivo. This review article summarizes the story and evolution of in vitro bioactivity testing methods using SBF, highlighting the influence of testing parameters (e.g., formulation and circulation of the solution) and material-related parameters (e.g., composition, geometry, texture). Suggestions for future methodological refinements are also provided at the end of the paper.

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