In Vivo Behavior of Calcium Phosphate Glasses with Controlled Solubility

2005 ◽  
Vol 284-286 ◽  
pp. 893-896 ◽  
Author(s):  
Melba Navarro ◽  
E.S. Sanzana ◽  
Josep A. Planell ◽  
M.P. Ginebra ◽  
P.A. Torres
Keyword(s):  
Calcium Phosphate ◽  
Biological Response ◽  
Phosphate Glasses ◽  
New Bone Formation ◽  
Tissue Engineering Scaffolds ◽  
Bone Fixation ◽  
Vitreous System ◽  
Fixation Devices ◽  
Good Biocompatibility

Resorbable calcium phosphate glasses offer interesting solutions in the biomedical field, as bone cavity fillers, drug delivery systems, biodegradable reinforcing phase in the case of composites for bone fixation devices and tissue engineering scaffolds. In this work, two different glass formulations in the systems 44.5CaO-44.5P2O5-(11-X)Na2O-XTiO2 (X=0or 5) have been elaborated. It is known that the incorporation or TiO2 into the vitreous system reduces considerably the solubility of the glasses. To study the material solubility effect on the in vivo response, glass particles of the two formulations were implanted in rabbits. Results showed that both glasses elicited a similar biological response and good biocompatibility. The percentage of new bone formation in the glasses was comparable to that obtained for the autologous bone (control) after 12 weeks of implantation. The materials showed to have an osteoconductive potential. Finally, this study showed that in spite of the solubility difference of the studied glasses, there were no significant differences in the in vivo response.

Biocompatibility and Osteogenesis of Calcium Phosphate Cement Scaffolds for Bone Tissue Engineering

2008 ◽  
Vol 47-50 ◽  
pp. 1383-1386 ◽  
Author(s):  
Han Guo ◽  
Jie Wei ◽  
Hang Kong ◽  
Chang Sheng Liu ◽  
Ke Feng Pan
Keyword(s):  
Stem Cells ◽  
Calcium Phosphate ◽  
Calcium Phosphate Cement ◽  
New Bone Formation ◽  
Negative Effects ◽  
Osteoblastic Phenotype ◽  
Proliferation And Differentiation ◽  
Good Biocompatibility

Porous calcium phosphate cement (CPC) scaffolds were successfully fabricated utilizing particle-leaching method. Mesenchymal stem cells (MSCs) were cultured, expanded and seeded on the scaffolds and the proliferation and differentiation of MSCs into osteoblastic phenotype were determined using MTT assay, ALP activity and ESEM. The results revealed that the CPC scaffolds were biocompatible and had no negative effects on the MSCs in vitro. The in vivo biocompatibility and osteogenicity of the scaffolds were investigated. Both pure scaffolds and MSCs/scaffold constructs were implanted in rabbit mandibles and studied histologically. The results showed that CPC scaffolds exhibited good biocompatibility and osteoconductivity. Moreover, the introduction of MSCs into the scaffolds dramatically enhanced the efficiency of new bone formation initially.

scholarly journals Spherical calcium phosphate nanoparticle fillers allow polymer processing of bone fixation devices with high bioactivity

2009 ◽  
Vol 50 (5) ◽  
pp. 952-960 ◽  
Author(s):  
Dirk Mohn ◽  
Duygu Ege ◽  
Kirill Feldman ◽  
Oliver D. Schneider ◽  
Thomas Imfeld ◽  
...  
Keyword(s):  
Calcium Phosphate ◽  
Polymer Processing ◽  
Bone Fixation ◽  
Fixation Devices ◽  
Calcium Phosphate Nanoparticle

In Vitro and In Vivo Evaluation Of Calcium Phosphate Bone Graft Substitutes

2010 ◽  
Vol 654-656 ◽  
pp. 2065-2070
Author(s):  
Ho Yeon Song ◽  
Young Hee Kim ◽  
Jyoti M. Anirban ◽  
In Seon Byun ◽  
Kyung A Kwak ◽  
...  
Keyword(s):  
Calcium Phosphate ◽  
Bone Formation ◽  
Bone Graft ◽  
Cellular Proliferation ◽  
New Bone Formation ◽  
Hydroxy Apatite ◽  
Defect Sites ◽  
Bone Graft Substitutes

Calcium phosphate ceramics such as hydroxy apatite (HA), β-tricalcium phosphate (β-TCP) and bicalcium phosphate (BCP) have been used as a bone graft biomaterial because of their good biocompatibility and similarity of chemical composition to natural bones. To increase the mechanical and osteoconductive properties, the granules and spongy type porous bone graft substitutes were prepared by fibrous monolithic process and polyurethane foam replica methods, respectively. The pore sizes obtained using these approaches ranged between 100-600 µm. The cytotoxicity, cellular proliferation, differentiation and ECM deposition on the bone graft substitutes were observed by SEM and confocal microscopy. Moreover, the scaffolds were implanted in the rabbit femur. New bone formation and biodegradation of bone graft were observed through follow-up X-ray, micro-CT analysis and histological findings. After several months (2, 3, 6, 12 and 24 months) of implantation, new bone formation and ingrowths were observed in defect sites of the animal by CaP ceramics and 2 to 3 times higher bone ingrowths were confirmed than that of the normal trabecular bones in terms of total bone volume (BV).

Effect of Particle Size on New Bone Formation In Vivo Using Calcium Phopshate Glass

2007 ◽  
Vol 330-332 ◽  
pp. 165-168
Author(s):  
Hyun Ju Moon ◽  
Racquel Z. LeGeros ◽  
Kyoung Nam Kim ◽  
Kwang Mahn Kim ◽  
Seong Ho Choi ◽  
...  
Keyword(s):  
Particle Size ◽  
Calcium Phosphate ◽  
Bone Formation ◽  
Phosphate Glass ◽  
Glass Powder ◽  
New Bone Formation ◽  
Sprague Dawley ◽  
Constant Weight ◽  
Calvarial Defects

The purpose of this study was to compare the bone regenerative effect of calcium phosphate glass according to the particle size in vivo. We prepared two different sizes, that is 400 μm and 40 μm, of calcium phosphate glass powder using the system CaO-CaF2-P2O5-MgO-ZnO. Critical-sized calvarial defects were created in 60 male Sprague-Dawley rats. The animals were divided into 3 groups of 20 animals each. Each defect was filled with a constant weight of 0.5 g calcium phosphate glass powder mixed with saline. As controls, the defect was left empty. The rats were sacrificed 2 or 8 weeks after postsurgery, and the results were evaluated using histological as well as histomorphometrical studies. The particle size of the calcium phosphate was crucial; 400 μm particles promoted new bone formation, while 40 μm particles inhibited it because of severe inflammation.

In Vivo Behavior of Injectable Fast-Setting Calcium Phosphate Cement

2007 ◽  
Vol 336-338 ◽  
pp. 1625-1627
Author(s):  
Li Min Dong ◽  
Chen Wang ◽  
Rui Liu ◽  
Jie Mo Tian ◽  
Qing Feng Zan
Keyword(s):  
Calcium Phosphate ◽  
Calcium Phosphate Cement ◽  
Femoral Condyle ◽  
Setting Time ◽  
Bone Defects ◽  
Control Group ◽  
Blank Control Group ◽  
Good Biocompatibility ◽  
The Right

The in vivo study was performed to evaluate the biocompatibility and osteogenous ability of injectable fast-setting calcium phosphate cement (CPC). Eighteen four-week-old New Zealand white rabbits were divided into six groups randomly, three in each group. According to the principle of selfcontrast at the same time, cavities of 5mm in diameter and 6mm in depth were drilled in femoral condyle of rabbits. The materials were implanted into cavities of the left leg, the right leg as the blank control group. Rabbits were sacrificed at 2, 4, 8, 12, 16 and 24 weeks after surgery. The microstructure of specimens was observed using ESEM. The results showed that injectable fast-setting CPC had good fluidity and plasticity; it could be injected into bone defects and fast-set in situ. The start setting time was 5-8 min and the compressive strength was 25-30 MPa. The CPC had good biocompatibility and osteoconductivity, and benefited to the repair of bone defects.

In Vivo Behavior of Calcium Phosphate Glasses with Controlled Solubility

2005 ◽  
pp. 893-896
Author(s):  
M. Navarro ◽  
E.S. Sanzana ◽  
Josep A. Planell ◽  
M.P. Ginebra ◽  
P.A. Torres
Keyword(s):  
Calcium Phosphate ◽  
Phosphate Glasses

scholarly journals Ex Vivo and In Vivo Analyses of Novel 3D-Printed Bone Substitute Scaffolds Incorporating Biphasic Calcium Phosphate Granules for Bone Regeneration

2021 ◽  
Vol 22 (7) ◽  
pp. 3588
Author(s):  
Franciska Oberdiek ◽  
Carlos Ivan Vargas ◽  
Patrick Rider ◽  
Milijana Batinic ◽  
Oliver Görke ◽  
...  
Keyword(s):  
Calcium Phosphate ◽  
Bone Formation ◽  
Bone Regeneration ◽  
Biphasic Calcium Phosphate ◽  
Ex Vivo ◽  
Proper Function ◽  
New Bone Formation ◽  
3D Printed ◽  
Implantation Model

(1) Background: The aim of this study was examining the ex vivo and in vivo properties of a composite made from polycaprolactone (PCL) and biphasic calcium phosphate (BCP) (synprint, ScientiFY GmbH) fabricated via fused deposition modelling (FDM); (2) Methods: Scaffolds were tested ex vivo for their mechanical properties using porous and solid designs. Subcutaneous implantation model analyzed the biocompatibility of PCL + BCP and PCL scaffolds. Calvaria implantation model analyzed the osteoconductive properties of PCL and PCL + BCP scaffolds compared to BCP as control group. Established histological, histopathological and histomorphometrical methods were performed to evaluate new bone formation.; (3) Results Mechanical testing demonstrated no significant differences between PCL and PCL + BCP for both designs. Similar biocompatibility was observed subcutaneously for PCL and PCL + BCP scaffolds. In the calvaria model, new bone formation was observed for all groups with largest new bone formation in the BCP group, followed by the PCL + BCP group, and the PCL group. This finding was influenced by the initial volume of biomaterial implanted and remaining volume after 90 days. All materials showed osteoconductive properties and PCL + BCP tailored the tissue responses towards higher cellular biodegradability. Moreover, this material combination led to a reduced swelling in PCL + BCP; (4) Conclusions: Altogether, the results show that the newly developed composite is biocompatible and leads to successful osteoconductive bone regeneration. The new biomaterial combines the structural stability provided by PCL with bioactive characteristics of BCP-based BSM. 3D-printed BSM provides an integration behavior in accordance with the concept of guided bone regeneration (GBR) by directing new bone growth for proper function and restoration.

scholarly journals Biocompatibility, degradability, bioactivity and osteogenesis of mesoporous/macroporous scaffolds of mesoporous diopside/poly( l -lactide) composite

2015 ◽  
Vol 12 (111) ◽  
pp. 20150507 ◽  
Author(s):  
Zhulin Liu ◽  
Jiajin Ji ◽  
Songchao Tang ◽  
Jun Qian ◽  
Yonggang Yan ◽  
...  
Keyword(s):  
Degradation Products ◽  
Immunohistochemical Analysis ◽  
Solution Casting ◽  
Macroporous Structure ◽  
New Bone Formation ◽  
Composite Scaffolds ◽  
Good Biocompatibility ◽  
Particulate Leaching ◽  
Acidic Degradation

Bioactive mesoporous diopside (m-DP) and poly( l -lactide) (PLLA) composite scaffolds with mesoporous/macroporous structure were prepared by the solution-casting and particulate-leaching method. The results demonstrated that the degradability and bioactivity of the mesoporous/macroporous scaffolds were significantly improved by incorporating m-DP into PLLA, and that the improvement was m-DP content-dependent. In addition, the scaffolds containing m-DP showed the ability to neutralize acidic degradation products and prevent the pH from dropping in the solution during the soaking period. Moreover, the scaffolds containing m-DP enhanced attachment, proliferation and alkaline phosphatase activity of MC3T3-E1 cells, which were also m-DP content-dependent. Furthermore, the histological and immunohistochemical analysis results showed that the scaffolds with m-DP significantly promoted new bone formation and improved the materials degraded in vivo , indicating good biocompatibility. The results suggested that the mesoporous/macroporous scaffolds of the m-DP/PLLA composite with osteogenesis had a potential for bone regeneration.

Cotton-Wool-Like Resorbable Bone Void Fillers Containing β-TCP and Calcium Carbonate Particles

2018 ◽  
Vol 782 ◽  
pp. 53-58 ◽  
Author(s):  
Naoki Osada ◽  
Masashi Makita ◽  
Yasutoshi Nishikawa ◽  
Toshihiro Kasuga
Keyword(s):  
Distal Femur ◽  
Cotton Wool ◽  
Micro Ct ◽  
New Bone Formation ◽  
Defect Sites ◽  
Electrospinning Method ◽  
New Zealand White Rabbits ◽  
Good Biocompatibility ◽  
Bone Void

Cotton-wool-like bioresorbable bone void fillers consisting of β-tricalcium phosphate (β-TCP), siloxane-containing vaterite (SiV) and poly (L-lactic acid) (PLLA) was prepared by an electrospinning method. The fibers, which were 50 ~ 150 μm-width with 10 ~ 30 μm-thickness, were entwined. The resulting cotton-wool-like material showed mechanical flexibility and excellent shapability; it showed easy, excellent mechanical-fixation in defects. The in vivo performance of this material was examined in the distal femur in New Zealand white rabbits. It was evaluated using micro CT and histologic analyses at time points of 6 and 12 weeks. These analyses of the defect sites verified normal healing response and new bone formation. The in vivo testing with rabbits showed good biocompatibility and excellent osteogenic ability.

scholarly journals Strategies for Enhancing Polyester-Based Materials for Bone Fixation Applications

Molecules ◽  
2021 ◽  
Vol 26 (4) ◽  
pp. 992
Author(s):  
Raasti Naseem ◽  
Charalampos Tzivelekis ◽  
Matthew J. German ◽  
Piergiorgio Gentile ◽  
Ana M. Ferreira ◽  
...  
Keyword(s):  
Particulate Composite ◽  
Surface Modifications ◽  
Polymer Blending ◽  
Bulk Properties ◽  
Bone Fixation ◽  
Fixation Devices ◽  
Surface Functionalisation ◽  
Initial Interaction ◽  
Primary Focus

Polyester-based materials are established options, regarding the manufacturing of bone fixation devices and devices in routine clinical use. This paper reviews the approaches researchers have taken to develop these materials to improve their mechanical and biological performances. Polymer blending, copolymerisation, and the use of particulates and fibre bioceramic materials to make composite materials and surface modifications have all been studied. Polymer blending, copolymerisation, and particulate composite approaches have been adopted commercially, with the primary focus on influencing the in vivo degradation rate. There are emerging opportunities in novel polymer blends and nanoscale particulate systems, to tune bulk properties, and, in terms of surface functionalisation, to optimise the initial interaction of devices with the implanted environment, offering the potential to improve the clinical performances of fracture fixation devices.

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