Study on Poly(β-Hydroxybutyrate-Co-β-Hydroxyvalerate)/Bioglass Composites as Scaffold for Tissue Engineering

The porous poly（β-hydroxybutyrate-co-β-hydroxyvalerate）（PHBV）/bioactive glasses （BG） tissue engineering scaffold was prepared in this article. The mineralization behavior of the porous PHBV/BG scaffold was observed in simulated body fluid (SBF). The ion concentration of calcium, silicon and phosphorus in different mineralization periods were tested by inductively coupled plasma (ICP). The formation of the hydroxyl carbonate apatite(HCA) layer on the scaffold surface was confirmed by the Fourier transform infrared(FTIR) spectroscopy and X-ray diffraction(XRD) apparatus. The micro morphology and porosity of the scaffold before and after mineralization were observed by scanning electron microscopy(SEM). The in vivo biological evaluation of the porous PHBV/BG scaffolds was carried out by implanting the scaffold into the rat muscle to test the biocompatibility. The in vivo result shows that the composite exhibit good biocompatibility. The porous PHBV/BG scaffold is suitable for tissue engineering.

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Assessment of Albumin ECM Accumulation and Inflammation as Novel In Vivo Diagnostic Targets for Multi-Target MR Imaging

Biology ◽

10.3390/biology10100964 ◽

2021 ◽

Vol 10 (10) ◽

pp. 964

Author(s):

Jana Möckel ◽

Julia Brangsch ◽

Carolin Reimann ◽

Jan O. Kaufmann ◽

Ingolf Sack ◽

...

Keyword(s):

Inductively Coupled Plasma ◽

Endothelial Damage ◽

Plaque Burden ◽

Signal Loss ◽

Inductively Coupled ◽

Before And After ◽

Plasma Mass Spectrometry ◽

Magnetic Resonance Imaging Mri ◽

Mri Study

Atherosclerosis is a progressive inflammatory vascular disease characterized by endothelial dysfunction and plaque burden. Extracellular matrix (ECM)-associated plasma proteins play an important role in disease development. Our magnetic resonance imaging (MRI) study investigates the feasibility of using two different molecular MRI probes for the simultaneous assessment of ECM-associated intraplaque albumin deposits caused by endothelial damage and progressive inflammation in atherosclerosis. Male apolipoprotein E-deficient (ApoE-/-)-mice were fed a high-fat diet (HFD) for 2 or 4 months. Another ApoE-/--group was treated with pravastatin and received a HFD for 4 months. T1- and T2*-weighted MRI was performed before and after albumin-specific MRI probe (gadofosveset) administration and a macrophage-specific contrast agent (ferumoxytol). Thereafter, laser ablation inductively coupled plasma mass spectrometry and histology were performed. With advancing atherosclerosis, albumin-based MRI signal enhancement and ferumoxytol-induced signal loss areas in T2*-weighted MRI increased. Significant correlations between contrast-to-noise-ratio (CNR) post-gadofosveset and albumin stain (R2 = 0.78, p < 0.05), and signal loss areas in T2*-weighted MRI with Perls’ Prussian blue stain (R2 = 0.83, p < 0.05) were observed. No interference of ferumoxytol with gadofosveset enhancement was detectable. Pravastatin led to decreased inflammation and intraplaque albumin. Multi-target MRI combining ferumoxytol and gadofosveset is a promising method to improve diagnosis and treatment monitoring in atherosclerosis.

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Rapid Catalyst Capture Enables Metal-Free Parahydrogen-Based Hyperpolarized Contrast Agents

10.26434/chemrxiv.6076175.v1 ◽

2018 ◽

Author(s):

Danila Barskiy ◽

Lucia Ke ◽

Xingyang Li ◽

Vincent Stevenson ◽

Nevin Widarman ◽

...

Keyword(s):

Magnetic Resonance ◽

Contrast Agents ◽

Inductively Coupled Plasma ◽

Organometallic Compounds ◽

Atomic Emission ◽

Platinum Group Metals ◽

Resonance Spectroscopy ◽

Plasma Atomic Emission ◽

Inductively Coupled

Hyperpolarization techniques based on the use of parahydrogen provide orders of magnitude signal enhancement for magnetic resonance spectroscopy and imaging. The main drawback limiting widespread applicability of parahydrogen-based techniques in biomedicine is the presence of organometallic compounds (the polarization transfer catalysts) in solution with hyperpolarized contrast agents. These catalysts are typically complexes of platinum-group metals and their administration in vivo should be avoided. Herein, we show how extraction of a hyperpolarized compound from an organic phase to an aqueous phase combined with a rapid (less than 10 seconds) Ir-based catalyst capture by metal scavenging agents can produce pure parahydrogen-based hyperpolarized contrast agents as demonstrated by high-resolution nuclear magnetic resonance (NMR) spectroscopy and inductively coupled plasma atomic emission spectroscopy (ICP-AES). The presented methodology enables fast and efficient means of producing pure hyperpolarized aqueous solutions for biomedical and other uses.

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Rapid Catalyst Capture Enables Metal-Free Parahydrogen-Based Hyperpolarized Contrast Agents

10.26434/chemrxiv.6076175 ◽

2018 ◽

Author(s):

Danila Barskiy ◽

Lucia Ke ◽

Xingyang Li ◽

Vincent Stevenson ◽

Nevin Widarman ◽

...

Keyword(s):

Magnetic Resonance ◽

Contrast Agents ◽

Inductively Coupled Plasma ◽

Organometallic Compounds ◽

Atomic Emission ◽

Platinum Group Metals ◽

Resonance Spectroscopy ◽

Plasma Atomic Emission ◽

Inductively Coupled

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Effect of wood biomass components on self-heating

Bioresources and Bioprocessing ◽

10.1186/s40643-021-00373-7 ◽

2021 ◽

Vol 8 (1) ◽

Author(s):

Nozomi Miyawaki ◽

Takashi Fukushima ◽

Takafumi Mizuno ◽

Miyao Inoue ◽

Kenji Takisawa

Keyword(s):

Moisture Content ◽

Inductively Coupled Plasma ◽

Optical Emission ◽

Chemical Oxidation ◽

Spontaneous Ignition ◽

Emission Spectrometry ◽

Inductively Coupled ◽

Self Heating ◽

Inorganic Substances ◽

Before And After

AbstractBiomass may ignite due to biological oxidation and chemical oxidation. If this phenomenon (spontaneous ignition) is controlled, it would be possible to produce biochar at a lower cost without the need for an external heat resource. We investigated if self-heating could be controlled by using sawdust and bark chips. When sawdust and bark chips were used under controlled conditions, the bark chips temperature increased to the torrefaction temperature. The ash content of bark chips was ~ 2%d.b. higher than that of sawdust; consequently, the inorganic substances contained in the bark chips might affect the self-heating. Self-heating was suppressed when inorganic substances were removed by washing with water. Therefore, the inorganic substances in the biomass might have affected self-heating. The inorganic element contents of the bark chips were measured by inductively coupled plasma optical emission spectrometry before and after washing. The potassium content of the bark chips was reduced remarkably by washing, and there was a possible influence of potassium on self-heating. Finally, the effect of moisture content on self-heating was investigated to obtain stable reactivity. Thus, at a moisture content of 40%w.b., a steady self-heating behavior may be realized.

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In vivo monitoring of the transfer kinetics of trace elements in animal brains with hyphenated inductively coupled plasma mass spectrometry techniques

Mass Spectrometry Reviews ◽

10.1002/mas.20240 ◽

2009 ◽

Vol 29 (3) ◽

pp. 392-424 ◽

Cited By ~ 15

Author(s):

Cheng-Kuan Su ◽

Yuh-Chang Sun ◽

Shun-Fen Tzeng ◽

Chung-Shi Yang ◽

Chih-Yen Wang ◽

...

Keyword(s):

Mass Spectrometry ◽

Trace Elements ◽

Inductively Coupled Plasma ◽

In Vivo Monitoring ◽

Inductively Coupled ◽

Plasma Mass Spectrometry ◽

Kinetics Of

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Ion Release from Dental Implants, Prosthetic Abutments and Crowns under Physiological and Acidic Conditions

Coatings ◽

10.3390/coatings11010098 ◽

2021 ◽

Vol 11 (1) ◽

pp. 98

Author(s):

María Arregui ◽

Florian Latour ◽

Francisco Javier Gil ◽

Román A. Pérez ◽

Luis Giner-Tarrida ◽

...

Keyword(s):

Lactic Acid ◽

Dental Implants ◽

Inductively Coupled Plasma ◽

Ion Concentration ◽

Ion Release ◽

Inductively Coupled ◽

Immersion Medium ◽

The Media ◽

Acidic Conditions ◽

Clinical Conditions

Ion release from dental implants and prosthetic restoration can affect osteointegration and implant viability over a long period of time. Therefore, the aim of this study was to study the ion release from implants and crowns, with and without intermediate anodized abutments, in two different media, simulating clinical conditions. The implants, intermediate prosthetic abutments and Cr–Co crowns were divided into two groups depending on the media: Hanks’ solution and 1% lactic acid, simulating body fluids and microbiologically conditioned fluids, respectively. The study followed the ISO 10271:2011 and 10993-15:2000 standards modified to simulate the replacement of fluids in the oral environment. The ions’ release was measured by inductively coupled plasma mass spectroscopy (ICP-EOS), and only aluminum, chromium, cobalt, titanium and vanadium were identified. Ion concentration was higher in lactic acid than in Hanks’ solution at all time points (p < 0.05). Only vanadium showed a very low ion release in lactic acid, with no statistically significant differences from the ion release in Hanks’ solution (p = 0.524). Both anodized abutments and the immersion medium influenced the release of ions and affected the corrosion of these structures. The presence of an intermediate anodized abutment also affected ion release, as the level of ions was lower in groups with this component.

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Lithium administered to pregnant, lactating and neonatal rats: entry into developing brain

Fluids and Barriers of the CNS ◽

10.1186/s12987-021-00285-w ◽

2021 ◽

Vol 18 (1) ◽

Author(s):

Shene Yi-Shiuan Chiou ◽

Kai Kysenius ◽

Yifan Huang ◽

Mark David Habgood ◽

Liam M. Koehn ◽

...

Keyword(s):

Mass Spectrometry ◽

Inductively Coupled Plasma ◽

Lithium Treatment ◽

Developing Brain ◽

Atpase Inhibitor ◽

Inductively Coupled ◽

Long Term Treatment ◽

Plasma Mass Spectrometry

Abstract Background Little is known about the extent of drug entry into developing brain, when administered to pregnant and lactating women. Lithium is commonly prescribed for bipolar disorder. Here we studied transfer of lithium given to dams, into blood, brain and cerebrospinal fluid (CSF) in embryonic and postnatal animals as well as adults. Methods Lithium chloride in a clinically relevant dose (3.2 mg/kg body weight) was injected intraperitoneally into pregnant (E15–18) and lactating dams (birth-P16/17) or directly into postnatal pups (P0–P16/17). Acute treatment involved a single injection; long-term treatment involved twice daily injections for the duration of the experiment. Following terminal anaesthesia blood plasma, CSF and brains were collected. Lithium levels and brain distribution were measured using Laser Ablation Inductively Coupled Plasma-Mass Spectrometry and total lithium levels were confirmed by Inductively Coupled Plasma-Mass Spectrometry. Results Lithium was detected in blood, CSF and brain of all fetal and postnatal pups following lithium treatment of dams. Its concentration in pups’ blood was consistently below that in maternal blood (30–35%) indicating significant protection by the placenta and breast tissue. However, much of the lithium that reached the fetus entered its brain. Levels of lithium in plasma fluctuated in different treatment groups but its concentration in CSF was stable at all ages, in agreement with known stable levels of endogenous ions in CSF. There was no significant increase of lithium transfer into CSF following application of Na+/K+ ATPase inhibitor (digoxin) in vivo, indicating that lithium transfer across choroid plexus epithelium is not likely to be via the Na+/K+ ATPase mechanism, at least early in development. Comparison with passive permeability markers suggested that in acute experiments lithium permeability was less than expected for diffusion but similar in long-term experiments at P2. Conclusions Information obtained on the distribution of lithium in developing brain provides a basis for studying possible deleterious effects on brain development and behaviour in offspring of mothers undergoing lithium therapy.

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In vitro Antimicrobial Effects of Virus Block, Which Contains Multiple Polyoxometalate Compounds, and Hygienic Effects of Virus Block-Supplemented Moist Hand Towels

Pharmacology ◽

10.1159/000500897 ◽

2019 ◽

Vol 104 (1-2) ◽

pp. 98-112 ◽

Cited By ~ 3

Author(s):

Katsuaki Dan ◽

Naohiro Katoh ◽

Takaaki Matsuoka ◽

Katsuyuki Fujinami

Keyword(s):

Inductively Coupled Plasma ◽

Metal Ion ◽

Bacterial Flora ◽

Atomic Emission ◽

Time Dependent ◽

Antimicrobial Effects ◽

Inductively Coupled ◽

Before And After ◽

Normal Bacterial Flora

Background: Historical evidence has verified the multifaceted antiviral efficacy of polyoxometalates (PMs). Methods: We carried out a study to investigate the antimicrobial effects of each of the 5 substances comprising virus block (VB): 3 PMs that have antibacterial and antiviral activity, an antibiotic agent, and an antibacterial agent. We also investigated the effectiveness of the addition of VB to moist hand towels in a study involving 120 volunteers. The time-dependent changes in metal ion concentrations in aqueous VB solution were analyzed using inductively coupled plasma atomic emission spectroscopy. Results: The metal elements in the aqueous VB solution remained stable for 12 weeks without undergoing time-dependent changes. Discussion: Further investigations were performed to study hand hygiene using moist hand towels in daily life settings. To this end, 120 volunteers provided 240 specimens that were used to investigate the presence of antibacterial compounds on the volunteers’ hands before and after hand towel use. An aliquot of each specimen was suspended in phosphate-buffered saline and plated on agar media, and the number of colonies formed was counted. Normal bacterial flora found on the hands of the volunteers was investigated before and after the use of 4 different moist hand towels. Conclusions: The effects of VB and PMs were superior to those of commercial moist hand towels, indicating that effective data were obtained that may be useful for the practical application of the tested items.

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Thermoelectric Device Based on Vertical Silicon Nanowires for On-Chip Integration

Key Engineering Materials ◽

10.4028/www.scientific.net/kem.609-610.789 ◽

2014 ◽

Vol 609-610 ◽

pp. 789-795 ◽

Cited By ~ 1

Author(s):

Zhen Wang ◽

Yang Yang Qi ◽

Ming Liang Zhang ◽

An Ji ◽

Fu Hua Yang ◽

...

Keyword(s):

Silicon Nanowires ◽

Inductively Coupled Plasma ◽

Platinum Resistance Thermometer ◽

Thermoelectric Device ◽

Platinum Resistance ◽

Resistance Thermometer ◽

Inductively Coupled ◽

Fold Reduction ◽

Before And After ◽

On Chip

A fabricating process of prototype thermoelectric device based on vertical silicon nanowires (SiNWs) for on-chip integration was presented. The SiNWs with diameter of 200 nm and height of 1 μm were fabricated by electron beam lithography and inductively coupled plasma etching. The gaps between the NWs were filled by the spin-on glass, which isolated the top and bottom electrodes. A serpentine platinum resistance thermometer coil was formed on the NWs to create temperature gradient across the NWs and measure the temperature of the top of NWs. I-V characteristics of the vertical device before and after annealing were measured. The nonlinear I-V curves were obtained, but the annealed one demonstrated 1000-fold reduction in resistance than the unannealed one.

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The Absorption, Distribution, and Excretion of 18 Elements of Tibetan Medicine Qishiwei Zhenzhu Pills in Rats with Cerebral Ischemia

Evidence-based Complementary and Alternative Medicine ◽

10.1155/2021/4508533 ◽

2021 ◽

Vol 2021 ◽

pp. 1-11

Author(s):

Yinglian Song ◽

Ke Fu ◽

Dewei Zhang ◽

Min Xu ◽

Ruixia Wu ◽

...

Keyword(s):

Cerebral Ischemia ◽

Inductively Coupled Plasma ◽

Microwave Digestion ◽

Venous Blood ◽

Artery Occlusion ◽

Tibetan Medicine ◽

Inductively Coupled ◽

Icp Ms

The aim of this study is to determine 18 elements in Tibetan medicine Qishiwei Zhenzhu pills (QSW) and their absorption, distribution, and excretion in rats with cerebral ischemia. Microwave digestion and inductively coupled plasma mass spectrometry (ICP-MS) were used to determine 18 elements of QSW in simulated gastrointestinal (GI) juice. Rats were given QSW (66.68 mg/kg) followed by middle cerebral artery occlusion (MCAO). Sham rats received saline and were not subjected to MCAO. ICP-MS was applied to determine the content of 18 elements in hepatic venous blood, abdominal aortic blood, brain, liver, kidney, hair, urine, and feces 24 h after MCAO. In vitro results showed that the extraction rate of Mn, Cu, Sr, Pb, Au, and Hg of QSW in gastric juice (1 h) was higher than that in water, and the contents of Cu, Au, Sr, and As were higher in intestinal juice (4 h) than in water. In vivo results showed that the contents of elements in the blood were quite low, and QSW increased Ni, Cr, Sr, Co, and V in artery blood and decreased V in venous blood. Elements in the tissues were also low, and QSW increased brain Li but decreased Cr and Cd; QSW increased kidney Ag and Cs and liver Mn but decreased liver Ni. QSW increased urinary excretion of Li, Sr, Hg, Cs, and V; QSW increased Hg content in hair but decreased Ni. Stool is the main excretion pathway of the elements in QSW, with Ba, Mn, Sr, Cd, V, Cu, Cs, Li, Pb, Ag, Hg, Cr, As, and Co the highest. In summary, this study examined the distribution of 18 elements in QSW-treated MCAO rats. The accumulation of these elements in blood and tissues was extremely low, and the majority was excreted in feces within 24 h, highlighting the importance of the gut-microbiota-brain axis in QSW-mediated brain protection.

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