scholarly journals In vitro Antimicrobial Effects of Virus Block, Which Contains Multiple Polyoxometalate Compounds, and Hygienic Effects of Virus Block-Supplemented Moist Hand Towels

Pharmacology ◽  
2019 ◽  
Vol 104 (1-2) ◽  
pp. 98-112 ◽  
Author(s):  
Katsuaki Dan ◽  
Naohiro Katoh ◽  
Takaaki Matsuoka ◽  
Katsuyuki Fujinami
Keyword(s):  
Inductively Coupled Plasma ◽  
Metal Ion ◽  
Bacterial Flora ◽  
Atomic Emission ◽  
Time Dependent ◽  
Antimicrobial Effects ◽  
Inductively Coupled ◽  
Before And After ◽  
Normal Bacterial Flora

Background: Historical evidence has verified the multifaceted antiviral efficacy of polyoxometalates (PMs). Methods: We carried out a study to investigate the antimicrobial effects of each of the 5 substances comprising virus block (VB): 3 PMs that have antibacterial and antiviral activity, an antibiotic agent, and an antibacterial agent. We also investigated the effectiveness of the addition of VB to moist hand towels in a study involving 120 volunteers. The time-dependent changes in metal ion concentrations in aqueous VB solution were analyzed using inductively coupled plasma atomic emission spectroscopy. Results: The metal elements in the aqueous VB solution remained stable for 12 weeks without undergoing time-dependent changes. Discussion: Further investigations were performed to study hand hygiene using moist hand towels in daily life settings. To this end, 120 volunteers provided 240 specimens that were used to investigate the presence of antibacterial compounds on the volunteers’ hands before and after hand towel use. An aliquot of each specimen was suspended in phosphate-buffered saline and plated on agar media, and the number of colonies formed was counted. Normal bacterial flora found on the hands of the volunteers was investigated before and after the use of 4 different moist hand towels. Conclusions: The effects of VB and PMs were superior to those of commercial moist hand towels, indicating that effective data were obtained that may be useful for the practical application of the tested items.

Antibacterial Effects of Sol-Gel Derived Zinc-Containing Hydroxyapatite

2007 ◽  
Vol 361-363 ◽  
pp. 139-142 ◽  
Author(s):  
Xin Chang Shi ◽  
H.Z. Jiang ◽  
J. Xue ◽  
Yun Mao Liao ◽  
L.Y. Xiao ◽  
...  
Keyword(s):  
Inductively Coupled Plasma ◽  
Sol Gel ◽  
Brain Heart Infusion ◽  
Zinc Content ◽  
Atomic Emission ◽  
Biological Properties ◽  
Zinc Ions ◽  
Antibacterial Effects ◽  
Inductively Coupled

In order to modify the biological properties of pure hydroxyapatite (HAp), two kinds of zinc-containing HAp, which had zinc content of 5.10wt% and 2.54wt%, respectively, were synthesized via sol-gel technique. The obtained coatings were characterized by X-ray diffraction (XRD) and scanning electron microscope (SEM). Then the antibacterial effects were evaluated through bacteriostatic test on Streptococcus mutans (S.mutans) in vitro and zinc ions releasing ability was investigated through detecting the zinc ions concentration by inductively coupled plasma-atomic emission spectroscopy (ICP-AES). The obtained coatings were observed to possess typical apatite peaks in XRD patterns and high homogeneous and porous surfaces in SEM morphology. The coating layer demonstrated good releasing ability in Brain Heart Infusion (BHI) liquid and obviously inhibitory effects to the growth and proliferation of S. mutans. Based on the results obtained above, it is concluded that the sol-gel derived zinc-containing hydroxyapatite could be applied as an antibacterial effective biomaterial.

scholarly journals Metal ions leachables from fake orthodontic braces incubated in simulated body fluid

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Riyam Haleem ◽  
Noor Ayuni Ahmad Shafiai ◽  
Siti Noor Fazliah Mohd Noor
Keyword(s):  
Simulated Body Fluid ◽  
Metal Ions ◽  
Inductively Coupled Plasma ◽  
Metal Ion ◽  
Body Fluid ◽  
Atomic Emission ◽  
Analysis Data ◽  
Inductively Coupled ◽  
Significant Release ◽  
Control Samples

Abstract Background The demand for fake braces usage in Southeast Asia are increasing but lack of certification and information on fake braces as medical devices from regulated bodies raised a concern towards its safety. The aim of this study was to determine the types of metal ion leachable from removable fake braces based on heavy metal ions present in metallic materials, immersed in simulated body fluid (SBF) and analysed using inductively coupled plasma atomic emission spectroscopy. Methods Three sets of fake braces and one control were dissembled to only their brackets and archwires and immersed separately in SBF. They were placed in an incubator shaker at a temperature of 37 °C at 50 rpm. A 3.0 ml measurement of SBF was taken out from the sample containers at days 7, 14 and 28 and kept at − 20 °C for further analysis. Data were analysed using SPSS version 26.0 (IBM, Armonk, USA) (P < 0.05). Descriptive and one-way ANOVA analyses with Bonferroni post hoc tests were used to assess the significant differences between the metal ions released in SBF from the control samples and fake braces. Results All 23 elements under investigation except Si ions were detected from the control samples and fake braces. There were significant increased K ions and reduced levels of Mg ions from the fake archwires and brackets. Most ions released were less than 10 mg/L (Ti, V, Cr, Mn, Fe, Co, Ni, Cu, Zn, Mo, Cd, Pb, Al) or 1 mg/L (Li, Ba) into the SBF medium. Conclusion There were significant release of Ca and K ions from the fake samples. Elements such as Li, Ba, Ti, V, Cr, Mn, Fe, Co, Ni, Cu, Zn, Mo, Cd and Sb had increased in concentration at day 7 and the concentration plateaued until day 28.

scholarly journals The Protein Phosphatases of Synechocystis sp. Strain PCC 6803: Open Reading Frames sll1033 and sll1387 Encode Enzymes That Exhibit both Protein-Serine and Protein-Tyrosine Phosphatase Activity In Vitro

2005 ◽  
Vol 187 (17) ◽  
pp. 5877-5884 ◽  
Author(s):  
Renhui Li ◽  
M. Ben Potters ◽  
Liang Shi ◽  
Peter J. Kennelly
Keyword(s):  
Inductively Coupled Plasma ◽  
Metal Ion ◽  
Protein Phosphatases ◽  
Tyrosine Phosphatase ◽  
Maximal Activity ◽  
Open Reading Frames ◽  
Inductively Coupled ◽  
Reading Frames ◽  
Pcc 6803

ABSTRACT The open reading frames (ORFs) encoding two potential protein-serine/threonine phosphatases from the cyanobacterium Synechocystis sp. strain PCC 6803 were cloned and their protein products expressed in Escherichia coli cells. The product of ORF sll1033, SynPPM3, is a homologue of the PPM family of protein-serine/threonine phosphatases found in all eukaryotes as well as many members of the Bacteria. Surprisingly, the recombinant protein phosphatase dephosphorylated phosphotyrosine- as well as phosphoserine-containing proteins in vitro. While kinetic analyses indicate that the enzyme was more efficient at dephosphorylating the latter, replacement of Asp608 by asparagine enhanced activity toward a phosphotyrosine-containing protein fourfold. The product of ORF sll1387, SynPPP1, is the sole homolog of the PPP family of protein phosphatases encoded by the genome of Synechocystis sp. strain PCC 6803. Like many other bacterial PPPs, the enzyme dephosphorylated phosphoserine- and phosphotyrosine-containing proteins with comparable efficiencies. However, while previously described PPPs from prokaryotic organisms required the addition of exogenous metal ion cofactors, such as Mg2+ or Mn2+, for activity, recombinantly produced SynPPP1 displayed near-maximal activity in the absence of added metals. Inductively coupled plasma mass spectrometry indicated that recombinant SynPPP1 contained significant quantities, 0.32 to 0.44 mol/mole total, of Mg and Mn. In this respect, the cyanobacterial enzyme resembled eukaryotic members of the PPP family, which are metalloproteins. mRNA encoding SynPPP1 or SynPPM3 could be detected in cells grown under many, but not all, environmental conditions.

scholarly journals Collagen/silicocarnotite composites, cross-linked with chondroitin sulphate: In vitro bioactivity

2011 ◽  
Vol 5 (3) ◽  
pp. 161-170 ◽  
Author(s):  
Lachezar Radev ◽  
Vladimir Hristov ◽  
Irena Michailova ◽  
Maria Fernandes ◽  
Isabel Salvado
Keyword(s):  
Inductively Coupled Plasma ◽  
Chondroitin Sulphate ◽  
Atomic Emission ◽  
Red Shift ◽  
Structure Evolution ◽  
In Vitro Test ◽  
In Vitro Bioactivity ◽  
Before And After ◽  
Vitro Test

In this work we present the experimental results on synthesis, structure evolution and in vitro bioactivity of collagen- silicocarnotite-chondroitin sulphate composites. The obtained samples were synthesised by mixing collagen (C) and silicocarnotite (S) powder with C:S ratio of 75:25 and 25:75 wt.% in the presence of chondroitin sulphate (ChS). Collagen was diluted in 5M CH3COOH before mixing. The obtained materials were characterized by X-ray diffraction (XRD), Fourier-transform infrared (FTIR) spectroscopy and scanning electron microscopy (SEM) before and after in vitro test in 1.5 simulated body fluid. XRD of synthesized samples showed that before immersion carbonate containing hydroxyapatite (CO3HA) were formed in situ in the composites. FTIR depicts the presence of HPO4 2- and the 'red shift' of COO- and SO3 - from ChS was also observed. This 'red shift' could be attributed that the ChS prefers to chelate Ca2+ from partially dissolved S powder. SEM of the prepared samples show the presence of nanosized hydroxyapatite (HA) whiskers and flower-like HA assemblies. After in vitro test, XRD proved the presence of HA with well-defined crystallinity. According to the FTIR results B-type CO3HA can be observed. SEM of the precipitated layers show the presence of HA spheres. Inductively coupled plasma atomic emission spectroscopy results lead to a conclusion that after in vitro test of the prepared composites silicon containing carbonate substituted hydroxyapatite (Si-CO3HA) may be formed on the surface of the immersed samples.

scholarly journals Importance of determining macro- and microelemental composition of blood in pregnant women with threat of preterm birth

2020 ◽  
Vol 37 (1) ◽  
pp. 73-78
Author(s):  
N. Ya. Abdullaeva
Keyword(s):  
Preterm Birth ◽  
Pregnant Women ◽  
Inductively Coupled Plasma ◽  
Atomic Emission ◽  
Perinatal Period ◽  
Emission Spectrometry ◽  
Plasma Atomic Emission Spectrometry ◽  
Inductively Coupled ◽  
Icp Ms ◽  
Before And After

Aim. In order to establish the dependence of the threat preterm birth in pregnant women on the concentration of macro- and micronutrients, a comparative analysis of blood was carried out in 92 pregnant women aged 19 to 37 before and after treatment. Materials and methods. Quantitative elemental analysis of blood serum was performed for 8 elements: Ca, Cu, Fe, K, Mg, Na, Zn, Se using the method of inductively coupled plasma atomic emission spectrometry (ICP MS 7700e, Intertech. Corp., USA). Results. The conducted macro- and microelements content analysis revealed that during pregnancy, childbirth and perinatal period, a violation of their quantity is of no small importance. Conclusions. A reliably significant dependence of the threat of abortion, hypoxia, intrauterine growth retardation on the balance of macro- and microelements was noted.

scholarly journals In vitro bioactivity of glass-ceramic/fibroin composites

2017 ◽  
Vol 11 (2) ◽  
pp. 136-146 ◽  
Author(s):  
Lachezar Radev ◽  
Irena Michailova ◽  
Silviya Stateva ◽  
Diana Zaimova ◽  
Hristo Georgiev ◽  
...  
Keyword(s):  
Photoelectron Spectroscopy ◽  
Inductively Coupled Plasma ◽  
Atomic Emission ◽  
Xrd Analysis ◽  
In Vitro Tests ◽  
In Vitro Bioactivity ◽  
X Ray ◽  
Before And After ◽  
Static Conditions

Bioactive composite materials were prepared by mixing 20 wt.% of silk fibroin (SF) and 80 wt.% of glassceramics from CaO-SiO2-P2O5-MgO system. In vitro bioactivity of the prepared composites was evaluated in 1.5 simulated body fluid (1.5 SBF) in static conditions. The obtained samples before and after in vitro tests were characterized by X-ray diffraction (XRD) analysis, Fourier transform infrared spectroscopy (FTIR), and X-ray photoelectron spectroscopy (XPS). The changes in 1.5 SBF solutions after soaking the samples were evaluated by inductively coupled plasma atomic emission spectroscopy (ICP-AES). MG63 osteosarcoma cells were used for the biological experiments. The obtained experimental data proved that the synthesized composites exhibit excellent in vitro bioactivity.

Purification of Polyether Polyols with Low Unsaturation

2013 ◽  
Vol 781-784 ◽  
pp. 2361-2365
Author(s):  
Wei Hua Ma ◽  
Qin Zhong
Keyword(s):  
Inductively Coupled Plasma ◽  
Total Concentration ◽  
Weight Ratio ◽  
Atomic Emission ◽  
Emission Spectrometry ◽  
Plasma Atomic Emission Spectrometry ◽  
Residual Concentration ◽  
Cobalt Ions ◽  
Inductively Coupled ◽  
Before And After

The purification of polyether polyols with low unsaturation was studied. The removal effect of residual double metal cyanide in the polyols by the methods of adsorption and precipitation-filtration is characterized by the residual concentration of the zinc and cobalt ions which is measured by Inductively Coupled Plasma Atomic Emission Spectrometry (ICP-AES). Compared with precipitation-filtration, the method of adsorption was more practical and efficient for the purification of the polyols. And the optimum kind and amount of the adsorbent were obtained. The results showed that the combination of chelating fiber and attapulgus clay was the most favorable for the removal of zinc and cobalt ions and the appropriate weight ratio of chelating fiber and attapulgus clay was 1:1. The total concentration of zinc and cobalt ions can be reduced to lower than 1×10-6. And the amount of the adsorbent added was 3.33% by the weight of the polyether polyols. And through Scanning Electron Spectroscopy (SEM) the morphology of the chelating fiber was characterized before and after adsorption.

Ceramic Refractory Compositions with Biosolubility Characteristics

2015 ◽  
Vol 1765 ◽  
pp. 71-76
Author(s):  
Ma.G. Joaquín-Morales ◽  
G. Vargas-Gutiérrez ◽  
J.L. Rodríguez-Galicia ◽  
G.I. Vazquez-Carbajal ◽  
J. López-Cuevas
Keyword(s):  
Inductively Coupled Plasma ◽  
Atomic Emission ◽  
X Ray Diffraction ◽  
Plasma Atomic Emission ◽  
Powder Mixtures ◽  
X Ray ◽  
Inductively Coupled ◽  
Ceramic Refractory

ABSTRACTIn this work, we studied the dissolution of three different refractory compositions belonging to the ternary system SiO2-CaO-MgO into two Simulated Lung Fluids (SLF). The initial powder mixtures were uniaxially pressed and then sintered at 1300-1400 °C. The sintered samples were immersed for times from 1 to 21 days into a given SLF at 37 °C. The samples were characterized by X-Ray Diffraction (XRD) and Scanning Electron Microscopy (SEM). The dissolution of Ca2+, Mg2+ and Si4+ into the SLF was quantified by Inductively Coupled Plasma Atomic Emission Spectroscopy (ICP-AES). The in vitro studies suggested that all the considered materials had a potential to show a diminished biopersistence in vivo, due to reasons that depended on their chemical and phase composition.

scholarly journals Metal Release of Standard and Fake Orthodontic Braces: An In Vitro Study

2021 ◽  
Vol 20 (2) ◽  
Author(s):  
Siti Hajjar Nasir ◽  
Muhammad Syahmi Mohamad Amran ◽  
Muhammad Muaz Abidin Mustaffar
Keyword(s):  
Metal Ions ◽  
Inductively Coupled Plasma ◽  
Metal Ion ◽  
Artificial Saliva ◽  
In Vitro Study ◽  
Nickel Ions ◽  
Inductively Coupled ◽  
Significant Difference ◽  
Plasma Mass Spectrometry

INTRODUCTION: The growing demand for orthodontic braces among Malaysians has led to the development of fake braces. These fake braces services are illegal and their brackets are reported to be of inferior quality. Fake braces are constantly being exposed to the saliva intraorally, hence they are susceptible to corrosion. This study was conducted to investigate the release of metal ions as a result of corrosion from standard and fake orthodontic braces immersed in artificial saliva of different pH. MATERIALS AND METHODS: A total of six different types of brackets (three from standard and three from fake braces) were immersed in containers containing 5 mL of artificial saliva of pH 4.9 and pH 7.8. The samples were collected for analysis on day 1, day 14, and day 28 using Inductively-Coupled Plasma Mass Spectrometry (ICPMS) to evaluate the amount of metal ion released. Statistical analysis was performed to isolate the significant difference of metal ions released between two types of braces in different pH solutions. RESULTS: The release of aluminum, nickel, chromium, manganese and copper were observed and analyzed. Fake braces released the highest concentration of chromium, manganese, and nickel ions in both artificial saliva as compared to standard braces. Brackets immersed in pH 4.9 released a higher number of ions compared to pH 7.8. CONCLUSION: This study showed that fake braces released the highest concentration of metal ions as compared to standard braces. Both time and pH influenced the release of metal ions from orthodontic brackets.

scholarly journals Physicochemical Characteristics of Vinegar from Banana Peels and Commercial Vinegars before and after In Vitro Digestion

Processes ◽  
2021 ◽  
Vol 9 (7) ◽  
pp. 1193
Author(s):  
Ancuța Elena Prisacaru ◽  
Cristina Ghinea ◽  
Laura Carmen Apostol ◽  
Sorina Ropciuc ◽  
Vasile Florin Ursachi
Keyword(s):  
Inductively Coupled Plasma ◽  
In Vitro Digestion ◽  
Physicochemical Characteristics ◽  
Fermented Food ◽  
Banana Peel ◽  
Local Market ◽  
Inductively Coupled ◽  
Before And After ◽  
Plasma Mass Spectrometry

Vinegar is a fermented food with a diversity of uses seasoning, salad dressing and flavouring for foods. Since ancient times it is considered a remedy for health and today there are different types of vinegar on the market, and many others are under development. Determination of the physicochemical characteristics of the new types of vinegar is necessary in order to improve them. Therefore, the aim of this paper is to compare the physicochemical characteristics of vinegar obtained from banana peels (with or without boiling peels) at different ages, with those of commercial vinegars. The vinegar from banana peels was obtained and aged in our laboratory, while the commercial vinegars were purchased from a local market. The physicochemical characteristics of all the samples were investigated before and after gastric and intestinal digestion. Inductively coupled plasma mass spectrometry was used to determine the mineral content of the vinegars. Additionally, statistical analysis of the results was performed by applying a one-way analysis of variance. Results showed that vinegar obtained from banana peels is clearer and total dry extract values are lower than those of commercial vinegars. Banana peel vinegars have higher antioxidant activity and total polyphenol content similar to the commercial balsamic vinegars. This study advances the knowledge in the field of vinegar production by using raw agricultural by-products.

Sign in / Sign up

Export Citation Format

Share Document