Studies on Characteristics and Adsorption of BSA on Hydrogel Biomaterials

2007 ◽  
Vol 330-332 ◽  
pp. 901-904
Author(s):  
G.X. Tan ◽  
Ying De Cui ◽  
Ying Jun Wang
Keyword(s):  
Ionic Strength ◽  
Bovine Serum Albumin ◽  
Chemical Stability ◽  
Contact Lens ◽  
Bovine Serum ◽  
Protein Concentration ◽  
Radical Copolymerization ◽  
Vinyl Pyrrolidone ◽  
Equilibrium Water Content ◽  
Influence Of Ph

Hydrogel biomaterials were synthesized by radical copolymerization of N-vinyl pyrrolidone (NVP) and 2-hydroxyethylmathacrylate (HEMA), with azobisisobutyronitrile (AIBN) as an initiator, reacting at 60~70°C for 24 hours, which were designed as contact lens due to the good chemical stability and high biocompatibility. The absorbency of bovine serum albumin (BSA) was measured by the ultraviolet spectrophotometer. The influence of pH, initial protein concentration and ionic strength were investigated in detail. The results showed that the absorption of protein on hydrogel biomaterials increased with the immersing time increasing, and was stable during 4 days. The absorption of protein on hydrogel increased with the equilibrium water content increasing. The protein absorption on hydrogels reduced the permeability of the oxygen of the biomaterials.

Albumin-Induced Endocytic Vacuoles in Tetrahymena Pyrijormis

1975 ◽  
Vol 33 ◽  
pp. 694-695
Author(s):  
L. J. Brenner ◽  
D. G. Osborne ◽  
B. L. Schumaker
Keyword(s):  
Electron Microscopy ◽  
Bovine Serum Albumin ◽  
Bovine Serum ◽  
Protein Concentration ◽  
Tetrahymena Pyriformis ◽  
Sequence Of Events ◽  
Cytoplasmic Bodies ◽  
Food Vacuoles ◽  
Mouse Ascites ◽  
Normal Human

Exposure of the ciliate, Tetrahymena pyriformis, strain WH6, to normal human or rabbit sera or mouse ascites fluids induces the formation of large cytoplasmic bodies. By electron microscopy these (LB) are observed to be membrane-bounded structures, generally spherical and varying in size (Fig. 1), which do not resemble the food vacuoles of cells grown in proteinaceous broth. The possibility exists that the large bodies represent endocytic vacuoles containing material concentrated from the highly nutritive proteins and lipoproteins of the sera or ascites fluids. Tetrahymena mixed with bovine serum albumin or ovalbumin solutions having about the same protein concentration (7g/100 ml) as serum form endocytic vacuoles which bear little resemblance to the serum-induced LB. The albumin-induced structures (Fig. 2) are irregular in shape, rarely spherical, and have contents which vary in density and consistency. In this paper an attempt is made to formulate the sequence of events which might occur in the formation of the albumin-induced vacuoles.

scholarly journals Bridging interactions of proteins with silica nanoparticles: The influence of pH, ionic strength and protein concentration

Soft Matter ◽  
2014 ◽  
Vol 10 (5) ◽  
pp. 718-728 ◽  
Author(s):  
Bhuvnesh Bharti ◽  
Jens Meissner ◽  
Sabine H. L. Klapp ◽  
Gerhard H. Findenegg
Keyword(s):  
Ionic Strength ◽  
Silica Nanoparticles ◽  
Protein Concentration ◽  
Influence Of Ph

Influence of pH and micellar systems on the sensitized photo‐oxidation of bovine serum albumin

Luminescence ◽  
2019 ◽  
Vol 34 (3) ◽  
pp. 324-333 ◽  
Author(s):  
Eugenia Reynoso ◽  
R. Daniel Cacciari ◽  
Carlos A. Suchetti ◽  
Hernán A. Montejano ◽  
M. Alicia Biasutti
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Bovine Serum ◽  
Photo Oxidation ◽  
Micellar Systems ◽  
Influence Of Ph

scholarly journals The effect of ionic strength on the rheology of pH-induced bovine serum albumin/κ-carrageenan coacervates

LWT ◽  
2014 ◽  
Vol 59 (1) ◽  
pp. 356-360 ◽  
Author(s):  
Changhoon Chai ◽  
Jooyoung Lee ◽  
Qingrong Huang
Keyword(s):  
Ionic Strength ◽  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Bovine Serum
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