In Vitro Evaluation of RF Magnetron-Sputtered Calcium Phosphate Films on Titanium

2007 ◽  
Vol 352 ◽  
pp. 305-309
Author(s):  
Kyosuke Ueda ◽  
Takayuki Narushima ◽  
Tomoyuki Katsube ◽  
Hiroshi Kawamura ◽  
Takashi Goto
Keyword(s):  
Calcium Phosphate ◽  
Calcium Ions ◽  
Rf Magnetron Sputtering ◽  
Calcium Phosphate Coating ◽  
Apatite Formation ◽  
Coating Films ◽  
Before And After ◽  
Phosphate Buffered Saline ◽  
Titanium Substrates

Calcium phosphate coating films were fabricated on mirror-polished or blast-treated titanium substrates using radio-frequency (RF) magnetron sputtering and they were evaluated in vitro. Immersion tests for the films were conducted using phosphate-buffered saline (PBS(-)), and apatite formation and the elution of calcium ions from the films were investigated. The bonding strengths between the calcium phosphate films and titanium substrates before and after the immersion tests were evaluated. After the immersion tests, a decrease in the bonding strength was observed for the coating films on the mirror-polished titanium substrates, while that for the blast-treated titanium substrates was almost the same as that before the immersion tests.

Calcium Phosphate Coating on Blast-Treated Titanium Implants by RF Magnetron Sputtering

2009 ◽  
Vol 631-632 ◽  
pp. 211-216 ◽  
Author(s):  
Kyosuke Ueda ◽  
Takayuki Narushima ◽  
Takashi Goto ◽  
T. Katsube ◽  
Hironobu Nakagawa ◽  
...  
Keyword(s):  
Calcium Phosphate ◽  
Magnetron Sputtering ◽  
Bonding Strength ◽  
Rf Magnetron Sputtering ◽  
Titanium Implants ◽  
Calcium Phosphate Coating ◽  
Phosphate Coating ◽  
Coating Films

Calcium phosphate coating films were fabricated on Ti-6Al-4V plates and screw-type implants with a blast-treated surface using radiofrequency (RF) magnetron sputtering and were evaluated in vitro and in vivo. Amorphous calcium phosphate (ACP) and oxyapatite (OAp) films obtained in this study could cover the blast-treated substrate very efficiently, maintaining the surface roughness. For the in vitro evaluations of the calcium phosphate coating films, bonding strength and alkaline phosphatase (ALP) activity were examined. The bonding strength of the coating films to a blast-treated substrate exceeded 60 MPa, independent of film phases except for the film after post-heat-treatment in silica ampoule. When compared with an uncoated substrate, the increase in the ALP activity of osteoblastic SaOS-2 cells on a calcium phosphate coated substrate was confirmed by a cell culture test. The removal torque of screw-type Ti-6Al-4V implants with a blast-treated surface from the femur of Japanese white rabbit increased with the duration of implantation and it was statistically improved by coating an ACP film 2 weeks after implantation. The in vitro and in vivo studies suggested that the application of the sputtered ACP film as a coating on titanium implants was effective in improving their biocompatibility with bones.

Calcium Phosphate Coating on Titanium Using Dry Process

2010 ◽  
Vol 654-656 ◽  
pp. 2162-2167
Author(s):  
Takayuki Narushima ◽  
Kyosuke Ueda ◽  
Takashi Goto ◽  
Jun Kurihara ◽  
Hiroshi Kawamura
Keyword(s):  
Calcium Phosphate ◽  
Magnetron Sputtering ◽  
Bonding Strength ◽  
Rf Magnetron Sputtering ◽  
Calcium Phosphate Coating ◽  
Phosphate Coating ◽  
Coating Films ◽  
Removal Torque

Oxyapatite, amorphous calcium phosphate, and double-layered calcium phosphate coating films were fabricated on mirror-polished commercially pure titanium (CP Ti) and blasted Ti-6Al-4V alloy substrates by radiofrequency (RF) magnetron sputtering; the properties of these films were evaluated in vivo and in vitro. The bonding strength between the calcium phosphate films and the Ti substrates was higher than 50 MPa. This value is higher than the bonding strength reported in the case of plasma-sprayed calcium phosphate coating films fabricated on Ti substrates. The removal torque of screw-type blasted Ti-6Al-4V alloy implants in the femurs of Japanese white rabbits increased with the duration of implantation, and the removal torque values of the coated implants was observed to be higher than those of the non-coated implants. In vitro and in vivo studies indicate that coating Ti implants with calcium phosphate films using RF magnetron sputtering is effective in improving the bone compatibility of Ti implants. Finally, the factors that should be considered in fabricating biomedical coating films were discussed.

Study of Bio-Mineralization of BCP with Compensation of Calcium and Phosphate Ions

2009 ◽  
Vol 610-613 ◽  
pp. 1391-1394
Author(s):  
Hua De Zheng ◽  
Ying Jun Wang ◽  
Qiang Ma ◽  
Cheng Yun Ning ◽  
Xiao Feng Chen
Keyword(s):  
Free Energy ◽  
Calcium Phosphate ◽  
Calcium Ions ◽  
Biphasic Calcium Phosphate ◽  
Porous Ceramic ◽  
Apatite Layer ◽  
Apatite Formation ◽  
Phosphate Ions

In the present study, an Intelligent Multi-parameter Simulated Evaluation in vitro （IMSE system） was used to study the deposition properties of apatite formation on the surface of biphasic calcium phosphate porous ceramic (BCP) from static and dynamic r-SBF. Results showed that apatite formed on the surface of BCP from static and dynamic r-SBF differed between each other. In static r-SBF, ions were transferred by diffusion, which could not compensate the consuming of calcium ions, and mist apatite layer was formed on the surface of samples. But in the dynamic r-SBF, simulated fluid was adjusted precisely and flowed forcedly, the concentrations of ions were homogeneous; with the compensation of ions, calcium and phosphate were supersaturated, and the free energy of apatite formation was negative, bone-like apatite sheets were formed on the surface of samples.

scholarly journals Evaluation of Calcium Phosphate Coating Films on Titanium Fabricated Using RF Magnetron Sputtering

2007 ◽  
Vol 48 (3) ◽  
pp. 307-312 ◽  
Author(s):  
Kyosuke Ueda ◽  
Takayuki Narushima ◽  
Takashi Goto ◽  
Tomoyuki Katsube ◽  
Hironobu Nakagawa ◽  
...  
Keyword(s):  
Calcium Phosphate ◽  
Magnetron Sputtering ◽  
Rf Magnetron Sputtering ◽  
Calcium Phosphate Coating ◽  
Phosphate Coating ◽  
Coating Films

Calcium Phosphate Coating on Titanium by RF Magnetron Sputtering

2012 ◽  
pp. 223-233
Author(s):  
Takayuki Narushima ◽  
Kyosuke Ueda
Keyword(s):  
Calcium Phosphate ◽  
Magnetron Sputtering ◽  
Rf Magnetron Sputtering ◽  
Calcium Phosphate Coating ◽  
Phosphate Coating ◽  
Metallic Biomaterials ◽  
Phosphate Coatings ◽  
Calcium Phosphate Coatings

In this chapter, the authors discuss the fabrication and properties of calcium phosphate coatings on titanium (Ti) by radio-frequency (RF) magnetron sputtering. First, they address the necessity of surface modification of metallic biomaterials and the effectiveness of calcium phosphate coating. Next, they briefly review the processes used in the application of calcium phosphate coatings and present the effect of sputtering parameters on the phase and deposition rates of these coatings. Finally, the chapter discusses the performance of amorphous and crystalline (oxyapatite) calcium phosphate coatings on Ti based on in vitro and in vivo evaluations.

Calcium Phosphate Films Coated on Titanium by RF Magnetron Sputtering for Medical Applications

2007 ◽  
Vol 539-543 ◽  
pp. 551-556 ◽  
Author(s):  
Takayuki Narushima ◽  
Kyosuke Ueda ◽  
Takashi Goto ◽  
Tomoyuki Katsube ◽  
Hiroshi Kawamura ◽  
...  
Keyword(s):  
Calcium Phosphate ◽  
Magnetron Sputtering ◽  
Calcium Ion ◽  
Pure Titanium ◽  
Rf Magnetron Sputtering ◽  
Titanium Implants ◽  
Apatite Formation ◽  
Commercially Pure Titanium ◽  
Commercially Pure ◽  
Titanium Substrates

Calcium phosphate films were coated on commercially pure titanium substrates by radiofrequency magnetron sputtering using β-tricalcium phosphate targets. The films consisted of amorphous calcium phosphate and oxyapatite phases. Immersion tests of the films were carried out in Hanks’ solution and PBS(-), and apatite formation and calcium ion elution from the films were investigated. The titanium cylinders coated with calcium phosphate films were implanted into the mandibles of beagle dogs. These results suggest that coating with calcium phosphate improves the biocompatibility of titanium implants with bone tissue.

In vitro study of electron beam deposited calcium phosphate coating in simulated body fluid

2007 ◽  
Vol 22 (3) ◽  
pp. 621-626
Author(s):  
M. Hamdi ◽  
Ari Ide-Ektessabi ◽  
J.A. Toque
Keyword(s):  
Electron Beam ◽  
Calcium Phosphate ◽  
Simulated Body Fluid ◽  
Body Fluid ◽  
In Vitro Study ◽  
Calcium Phosphate Coating ◽  
Carbonate Content ◽  
Apatite Formation ◽  
Calcium Phosphate Coatings

Calcium phosphate coatings prepared using the technique of electron beam deposition were immersed in a simulated body fluid for different periods of time to determine their response in vitro. The amorphous as-deposited coatings dissolved completely after a few days of immersion. After annealing in air at 700 °C, the dissolution of a small amount of amorphous phase in the crystalline coatings promotes the precipitation of bonelike apatite on the recessed regions by increasing the local supersaturation of calcium and phosphate ions. Formation of apatite was confirmed by the x-ray diffraction peaks at (200), (211), and (203) planes which grew after immersion in simulated body fluid. Fourier transform infrared results conformed to this with the increase in intensity of the absorption band at 1450 cm−1, signifying the increase in carbonate content. Scanning electron microscopy results showed spherical-shaped apatite nucleated on dissolved surface after 8 days of immersion. Sixteen days after immersion, almost 80% of the surface area was covered with apatite formation and grew to coalesce between neighboring particles forming an integrated platelike layer after 28 days. No obvious detachment between the grown layer and the underlying coating was observed.

scholarly journals Fabrication and bioresorbability of Ag- and Ta-containing amorphous calcium phosphate films formed on titanium substrates by RF magnetron sputtering

2020 ◽  
Vol 321 ◽  
pp. 05007
Author(s):  
Jun Wu ◽  
Kyosuke Ueda ◽  
Takayuki Narushima
Keyword(s):  
Calcium Phosphate ◽  
Magnetron Sputtering ◽  
Amorphous Calcium Phosphate ◽  
Biomedical Applications ◽  
Rf Magnetron Sputtering ◽  
Coating Films ◽  
Depth Direction ◽  
Simulated Body Fluids ◽  
Titanium Substrates ◽  
Hcl Solution

Silver (Ag)- and tantalum (Ta)-containing amorphous calcium phosphate (ACP) coating films were fabricated on titanium substrates by radiofrequency magnetron sputtering for biomedical applications. The sputtering targets were hot-pressed into 10 mol% Ag-containing ß-tricalcium phosphate sintered compacts with and without 8 mol% Ta. The fabricated ACP coating films were dense and smooth, with all elements distributed homogeneously along the depth direction. In addition, Ag existed as ions in the ACP regardless of the presence of Ta. The resorbability of the Ag-containing ACP coating films in simulated body fluids was suppressed by the addition of Ta. The concentration of detected Ag ions was higher in diluted nutrient broth solution than in Tris-HCl solution.

scholarly journals Amorphous–Crystalline Calcium Phosphate Coating Promotes In Vitro Growth of Tumor-Derived Jurkat T Cells Activated by Anti-CD2/CD3/CD28 Antibodies

Materials ◽  
2021 ◽  
Vol 14 (13) ◽  
pp. 3693
Author(s):  
Yurii P. Sharkeev ◽  
Ekaterina G. Komarova ◽  
Valentina V. Chebodaeva ◽  
Mariya B. Sedelnikova ◽  
Aleksandr M. Zakharenko ◽  
...  
Keyword(s):  
T Cells ◽  
Calcium Phosphate ◽  
Biological Effects ◽  
Electrical Potential ◽  
Biological Properties ◽  
Modern Trend ◽  
Calcium Phosphate Coating ◽  
Jurkat T Cells ◽  
Micro Arc Oxidation

A modern trend in traumatology, orthopedics, and implantology is the development of materials and coatings with an amorphous–crystalline structure that exhibits excellent biocopatibility. The structure and physico–chemical and biological properties of calcium phosphate (CaP) coatings deposited on Ti plates using the micro-arc oxidation (MAO) method under different voltages (200, 250, and 300 V) were studied. Amorphous, nanocrystalline, and microcrystalline statesof CaHPO4 and β-Ca2P2O7were observed in the coatings using TEM and XRD. The increase in MAO voltage resulted in augmentation of the surface roughness Ra from 2.5 to 6.5 µm, mass from 10 to 25 mg, thickness from 50 to 105 µm, and Ca/P ratio from 0.3 to 0.6. The electrical potential (EP) of the CaP coatings changed from −456 to −535 mV, while the zeta potential (ZP) decreased from −53 to −40 mV following an increase in the values of the MAO voltage. Numerous correlations of physical and chemical indices of CaP coatings were estimated. A decrease in the ZP magnitudes of CaP coatings deposited at 200–250 V was strongly associated with elevated hTERT expression in tumor-derived Jurkat T cells preliminarily activated with anti-CD2/CD3/CD28 antibodies and then contacted in vitro with CaP-coated samples for 14 days. In turn, in vitro survival of CD4+ subsets was enhanced, with proinflammatory cytokine secretion of activated Jurkat T cells. Thus, the applied MAO voltage allowed the regulation of the physicochemical properties of amorphous–crystalline CaP-coatings on Ti substrates to a certain extent. This method may be used as a technological mechanism to trigger the behavior of cells through contact with micro-arc CaP coatings. The possible role of negative ZP and Ca2+ as effectors of the biological effects of amorphous–crystalline CaP coatings is discussed. Micro-arc CaP coatings should be carefully tested to determine their suitability for use in patients with chronic lymphoid malignancies.

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