The Experimental Study of Culture of Osteoblast-Like Cells (MG-63) on Nanometer Magnetic Composite Substrate In Vitro

2007 ◽  
Vol 361-363 ◽  
pp. 1103-1106
Author(s):  
M.Q. Wang ◽  
P. Hou ◽  
C.Y. Bao ◽  
Jie Weng ◽  
Wei Li
Keyword(s):  
Experimental Study ◽  
Cell Proliferation ◽  
Cell Morphology ◽  
Magnetic Composite ◽  
Alp Activity ◽  
Mtt Method ◽  
Composite Substrate ◽  
Typical Cell ◽  
Seed Cell

The purpose of this study was to evaluate the cytotoxicity of nanometer magnetic composite via being cultured with osteoblast-like cells (MG-63). The osteoblast-like cell (MG-63) was utilized as the seed cell. The cells were cultured on the surface of nanometer magnetic composite. After union culturing, on the day 1, 3, 5, 7, the cytotoxicity of nanometer magnetic composite was evaluated by MTT method and the cell morphology was observed by SEM. Meanwhile, on the day 4, 7, 10, the alkaline phosphatase (ALP) activity was tested respectively. These results demonstrated that the typical cell morphology could be observed when the osteoblast-like cells were cultured on nanometer magnetic composite substrate in vitro. Cell proliferation and ALP activity became higher as the prolongation of cultivate time in the group of nanometer magnetic composite. The study showed that nanometer magnetic composite had a little inhibition to cell proliferation and ALP activity in vitro cell culture, as compared with the chitin and PLA fiber substrate. So the feasibility of nanometer magnetic composite as scaffold for bone tissue engineering should be studied further.

Time-lapse Phase-contrast Microphotography of Cell Populations as a Basis for Improvement of In Vitro Toxicity Assessment

1992 ◽  
Vol 20 (2) ◽  
pp. 302-306
Author(s):  
Miroslav Červinka
Keyword(s):  
Cell Proliferation ◽  
Cell Morphology ◽  
Video Recording ◽  
Toxicity Testing ◽  
Time Lapse ◽  
Time Dependent ◽  
In Vitro Toxicity ◽  
Simple Modification ◽  
Pertinent Data

Recent trends in the field of in vitro toxicology have centred around the validation of in vitro methods. The ultimate goal is to obtain pertinent data with the minimum of effort. In our laboratory, we have used toxicological methods based on the evaluation of cell morphology and cell proliferation. A method suitable for this purpose is time-lapse microcinematographic (or video) recording of cellular changes, which we used for many years. For practical in vitro toxicity testing, however, this method is far too complicated. Therefore, we have tried to develop a simple modification for the evaluation of cell morphology and cell proliferation, which would still allow for a basic time-dependent analysis. Comparison of detailed microcinematographic analysis with analysis according to our new proliferation assay is demonstrated with cisplatin as the toxicant. We believe that a time-dependent approach could improve the in vitro assessment of toxicity.

scholarly journals Surface topography of hydroxyapatite affects ROS17/2.8 cells response

2002 ◽  
Vol 16 (3) ◽  
pp. 209-215 ◽  
Author(s):  
Adalberto Luiz Rosa ◽  
Márcio Mateus Beloti ◽  
Richard van Noort ◽  
Paul Vincent Hatton ◽  
Anne Jane Devlin
Keyword(s):  
Cell Proliferation ◽  
Protein Content ◽  
Surface Topography ◽  
Cell Morphology ◽  
Total Protein ◽  
Cell Attachment ◽  
Maxillofacial Surgery ◽  
Total Protein Content ◽  
Alp Activity ◽  
Powder Pressing

Hydroxyapatite (HA) has been used in orthopedic, dental, and maxillofacial surgery as a bone substitute. The aim of this investigation was to study the effect of surface topography produced by the presence of microporosity on cell response, evaluating: cell attachment, cell morphology, cell proliferation, total protein content, and alkaline phosphatase (ALP) activity. HA discs with different percentages of microporosity (< 5%, 15%, and 30%) were confected by means of the combination of uniaxial powder pressing and different sintering conditions. ROS17/2.8 cells were cultured on HA discs. For the evaluation of attachment, cells were cultured for two hours. Cell morphology was evaluated after seven days. After seven and fourteen days, cell proliferation, total protein content, and ALP activity were measured. Data were compared by means of ANOVA and Duncan’s multiple range test, when appropriate. Cell attachment (p = 0.11) and total protein content (p = 0.31) were not affected by surface topography. Proliferation after 7 and 14 days (p = 0.0007 and p = 0.003, respectively), and ALP activity (p = 0.0007) were both significantly decreased by the most irregular surface (HA30). These results suggest that initial cell events were not affected by surface topography, while surfaces with more regular topography, as those present in HA with 15% or less of microporosity, favored intermediary and final events such as cell proliferation and ALP activity.

scholarly journals Characterization of the iPSC-derived conditioned medium that promotes the growth of bovine corneal endothelial cells

PeerJ ◽  
2019 ◽  
Vol 7 ◽  
pp. e6734
Author(s):  
Qing Liu ◽  
Yonglong Guo ◽  
Shiwei Liu ◽  
Peiyuan Wang ◽  
Yunxia Xue ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Endothelial Cells ◽  
Conditioned Medium ◽  
Cell Morphology ◽  
Cell Cycle Progression ◽  
Cellular Proliferation ◽  
Activin A ◽  
Corneal Endothelial Cells ◽  
Induced Pluripotent

Corneal endothelial cells (CECs) maintain corneal transparency and visual acuity. However, the limited proliferative capability of these cells in vitro has prompted researchers to find efficient culturing techniques for them. The aim of our study was to evaluate the use of conditioned medium (CM) obtained from induced pluripotent stem cells (iPSCs) as a source for the effective proliferation of bovine CECs (B-CECs). In our study, the proliferative ability of B-CECs was moderately enhanced when the cells were grown in 25% iPSC conditioned medium (iPSC-CM). Additionally, hexagonal cell morphology was maintained until passage 4, as opposed to the irregular and enlarged shape observed in control corneal endothelial medium (CEM). B-CECs in both the 25% iPSC-CM and CEM groups expressed and Na+-K+-ATPase. The gene expression levels of NIFK, Na+-K+-ATPase, Col4A and Col8A and the percentage of cells entering S and G2 phases were higher in the iPSC-CM group. The number of apoptotic cells also decreased in the iPSC-CM group. In comparison to the control cultures, iPSC-CM facilitated cell migration, and these cells showed better barrier functions after several passages. The mechanism of cell proliferation mediated by iPSC-CM was also investigated, and phosphorylation of Akt was observed in B-CECs after exposure to iPSC-CM and showed sustained phosphorylation induced for up to 180 min in iPSC-CM. Our findings indicate that iPSC-CM may employ PI3-kinase signaling in regulating cell cycle progression, which can lead to enhanced cellular proliferation. Effective component analysis of the CM showed that in the iPSC-CM group, the expression of activin-A was significantly increased. If activin-A is added as a supplement, it could help to maintain the morphology of the cells, similar to that of CM. Hence, we conclude that activin-A is one of the effective components of CM in promoting cell proliferation and maintaining cell morphology.

Development of functionalized multi-walled carbon nanotube-based polysaccharide–hydroxyapatite scaffolds for bone tissue engineering

RSC Advances ◽  
2016 ◽  
Vol 6 (85) ◽  
pp. 82385-82393 ◽  
Author(s):  
R. Rajesh ◽  
Y. Dominic Ravichandran ◽  
M. Jeevan Kumar Reddy ◽  
Sung Hun Ryu ◽  
A. M. Shanmugharaj
Keyword(s):  
Tissue Engineering ◽  
Cell Proliferation ◽  
Carbon Nanotube ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Gellan Gum ◽  
Alp Activity ◽  
Multi Walled Carbon Nanotube ◽  
First Time

fMWCNT–amylopectin–HAP and fMWCNT–gellan gum–HAP were prepared and characterized and their in vitro cell proliferation and ALP activity were checked for the first time.

Effect of Simvastatin on Bone Marrow Mesenchymal Stem Cells in Osteoporosis Rats

2019 ◽  
Vol 9 (9) ◽  
pp. 1311-1316
Author(s):  
Yuechuang Liang ◽  
Liang Ma ◽  
Yu Wu ◽  
Youwei Tian ◽  
Dongyue Li ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Signaling Pathway ◽  
Caspase 3 ◽  
Lipid Lowering ◽  
Control Group ◽  
Activity Increase ◽  
Promote Cell Proliferation ◽  
Alp Activity ◽  
The Difference

Osteoporosis (OP) is a common and frequently-occurring disease in orthopedics. BMSCs play a role in OP. Simvastatin (SVA) is a commonly used lipid-lowering drug, but its role in OP remains unclear. Our study intends to assess SVA’s effect on BMSCs in osteoporosis rats. SD rats were randomly and equally divided into control group and OP group. BMSCs in control group and OP group were cultured in vitro treated with 5 μM and 10 μM SVA followed by analysis of cell proliferation by MTT assay, apoptosis activity by Caspase 3 activity, Wnt5/TGF-β signaling pathway protein expression by Western blot, ALP activity; Runx2 and OC expression by Real time PCR as well as BMP-2 and TGF-β secretion by ELISA. OP rat BMSCs showed significantly inhibited cell proliferation, increased Caspase 3 activity, decreased Wnt5, Runx2 and OC expression and ALP activity, as well as reudced BMP-2 and TGF-β secretion (P < 0.05). SVA can promote cell proliferation, inhibit Caspase 3 activity, increase Wnt5, Runx2 and OC expression and ALP activity, as well as promote BMP-2 and TGF-β secretion in OP rat BMSCs. Compared with OP group, the difference was statistically significant with more significant changes with increasing concentration (P < 0.05). Simvastatin activates Wnt5/TGF-β signaling pathway, regulates BMSCs proliferation and apoptosis and promotes their differentiation into osteogenic direction in OP rats.

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