The Interaction between Human Osteoblastic Cells and Titanium Anodized with Sulphuric Acid Coated or not with Human Plasma Fibronectin

2008 ◽  
Vol 396-398 ◽  
pp. 389-392
Author(s):  
Aline Raybolt dos Santos ◽  
Fernando Costa e Silva ◽  
Gloria Dulce de Almeida Soares

In this study, commercially pure titanium (Ti-cp) sheets grade 2 were used as substrates. To investigate the role of composition and characteristics of titanium surface oxides in cellular behavior of osteoblasts, the surface of titanium were modified by anodic oxidation with sulphuric acid. Subsequently were treated part of anodized samples with FN and cultured with osteoblast cells for 30, 120 and 240 minutes. The chemical composition and topography have influenced the interaction between fibronectin and substrate. Cells were found more associated to the surfaces that have been submitted to anodization and FN coating than to the related controls.

2014 ◽  
Vol 28 (12) ◽  
pp. 1207-1218 ◽  
Author(s):  
Miguel A. Fernández-Rodríguez ◽  
Alda Y. Sánchez-Treviño ◽  
Elvira De Luna-Bertos ◽  
Javier Ramos-Torrecillas ◽  
Olga García-Martínez ◽  
...  

2014 ◽  
Vol 8 (1) ◽  
pp. 114-119 ◽  
Author(s):  
In-Sung Yeo

Screw-shaped endosseous implants that have a turned surface of commercially pure titanium have a disadvantage of requiring a long time for osseointegration while those implants have shown long-term clinical success in single and multiple restorations. Titanium implant surfaces have been modified in various ways to improve biocompatibility and accelerate osseointegration, which results in a shorter edentulous period for a patient. This article reviewed some important modified titanium surfaces, exploring the in vitro, in vivo and clinical results that numerous comparison studies reported. Several methods are widely used to modify the topography or chemistry of titanium surface, including blasting, acid etching, anodic oxidation, fluoride treatment, and calcium phosphate coating. Such modified surfaces demonstrate faster and stronger osseointegration than the turned commercially pure titanium surface. However, there have been many studies finding no significant differences in in vivo bone responses among the modified surfaces. Considering those in vivo results, physical properties like roughening by sandblasting and acid etching may be major contributors to favorable bone response in biological environments over chemical properties obtained from various modifications including fluoride treatment and calcium phosphate application. Recently, hydrophilic properties added to the roughened surfaces or some osteogenic peptides coated on the surfaces have shown higher biocompatibility and have induced faster osseointegration, compared to the existing modified surfaces. However, the long-term clinical studies about those innovative surfaces are still lacking.


2020 ◽  
Vol 169 ◽  
pp. 110640
Author(s):  
Ehsan Farabi ◽  
Vahid Tari ◽  
Peter D. Hodgson ◽  
Gregory S. Rohrer ◽  
Hossein Beladi

2013 ◽  
Vol 753 ◽  
pp. 289-292
Author(s):  
Mariusz Jedrychowski ◽  
Jacek Tarasiuk ◽  
Brigitte Bacroix

EBSD investigation of texture and microstructure evolution during a complete thermomechanical treatment of commercially pure titanium (HCP-Ti) is presented. Titanium was cold rolled to reach various degrees of thickness reduction: 20%, 40% and 60%. Next, annealing in air atmosphere was conducted at different conditions to achieve the recrystallized state. EBSD topological maps were measured on RD-TD and RD-ND surface of each sample. Strong heterogeneity of deformed titanium microstructures is described with focus on the important role of twinning mechanisms. Texture evolution in investigated titanium appears to be limited, especially in recrystallized state. However some subtle mechanisms are discussed.


2012 ◽  
Vol 23 (5) ◽  
pp. 496-501 ◽  
Author(s):  
Marcelo Bighetti Toniollo ◽  
Rodrigo Galo ◽  
Ana Paula Macedo ◽  
Renata Cristina Silveira Rodrigues ◽  
Ricardo Faria Ribeiro ◽  
...  

The effects of fluoride, which is present in different oral hygiene products, deserve more investigation because little is known about their impact on the surface of titanium, which is largely used in Implantology. This study evaluated the surface of commercially pure titanium (cpTi) after exposure to different concentrations of sodium fluoride (NaF). The hypothesis tested in this study was that different concentrations of NaF applied at different time intervals can affect the titanium surface in different ways. The treatments resulted in the following groups: GA (control): immersion in distilled water; GB: immersion in 0.05% NaF for 3 min daily; GC: immersion in 0.2% NaF for 3 min daily; GD: immersion in 0.05% NaF for 3 min every 2 weeks; and GE: immersion in 0.2% NaF for 3 min every 2 weeks. The experiment lasted 60 days. Roughness was measured initially and every 15 days subsequently up to 60 days. After 60 days, corrosion analysis and anodic polarization were done. The samples were examined by scanning electron microscopy (SEM). The roughness data were analyzed by ANOVA and there was no significant difference among groups and among time intervals. The corrosion data (i corr) were analyzed by the Mann-Whitney test, and significant differences were found between GA and GC, GB and GC, GC and GD, GC and GE. SEM micrographs showed that the titanium surface exposed to NaF presented corrosion that varied with the different concentrations. This study suggests that the use of 0.05% NaF solution on cpTi is safe, whereas the 0.2% NaF solution should be carefully evaluated with regard to its daily use.


2012 ◽  
Vol 33 (3) ◽  
pp. 184-189 ◽  
Author(s):  
B. P. Gritsenko ◽  
Yu. F. Ivanov ◽  
N. N. Koval’ ◽  
K. V. Krukovskii ◽  
N. V. Girsova ◽  
...  

2007 ◽  
Vol 330-332 ◽  
pp. 333-336 ◽  
Author(s):  
Xiao Xiao Cai ◽  
Ping Gong ◽  
Yi Man ◽  
Zhi Qing Chen ◽  
Gang He

This research was aimed at the construction and characterization of nano-FHA bioceramic coating on titanium surface. Nano-FHA coating was constructed on the surface of commercially pure titanium by sol-gel route. X-ray diffraction (XRD), scanning electromicroscope (SEM) and dissolution test was employed to characterize the obtained coating. In vitro cellular responses of osteoblasts to the coating were also evaluated by MTT assay, ALP assay and SEM observation. Conventional HA coatings and commercially pure titanium (cpTi) were taken as control. Results show the nano-FHA bioceramic coating has good crystallization and homogeneous, nano-scale surface morphology. The dissolution rate of the coating is favorable. The in vitro osteoblasts culture exhibits satisfactory bioactivity.


2003 ◽  
Vol 12 (2) ◽  
pp. 182-187 ◽  
Author(s):  
Edmur Pereira Buzzá ◽  
Jamil Awad Shibli ◽  
Roberto Henrique Barbeiro ◽  
José Ricardo de Albergaria Barbosa

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