Biological Safety Assessment of a Colored Zirconia Ceramic: Cell Toxicity and Skin Sensitivity Tests

2011 ◽  
Vol 492 ◽  
pp. 509-512
Author(s):  
Huai Xiu Lu ◽  
Bin Deng ◽  
Long Quan Shao ◽  
Yuan Fu Yi ◽  
Jie Liu ◽  
...  
Keyword(s):  
Guinea Pig ◽  
Skin Irritation ◽  
Skin Inflammation ◽  
Intradermal Injection ◽  
Test Material ◽  
Zirconia Ceramic ◽  
Blue Dye ◽  
Skin Sensitivity ◽  
Filtration Method ◽  
Biological Safety

Objective: To evaluate the biological safety of a colored zirconia ceramic in terms of cellular toxicity and a skin sensitivity test. Methods: the cytotoxicity of the ceramic was evaluated by a molecular filtration method and skin irritation activity of the composite was assessed via intradermal injection of a guinea pig test solution, by inducing, enhancing, and stimulating an allergic response after local tissue exposure. Results: cell-coated filter paper in contact with test material showed the appearance of intracellular blue dye at the same staining density as in control samples, leading to a 0 toxicity rating. Guinea pigs showed no obvious erythema or edema from the irritation test, such that the assessment of colored zirconia ceramic skin allergy response in guinea pig was 0%. Conclusion: this colored zirconia ceramic showed low cytotoxicity and elicited no allergic skin inflammation response, indicating good overall biological safety under these conditions.

A New Type of Colored Alumina/Glass Composite Biological Safety Assessment - Oral Mucous Membrane Irritation and Skin Sensitivity Tests

2010 ◽  
Vol 177 ◽  
pp. 462-465
Author(s):  
Huai Xiu Lu ◽  
Bin Deng ◽  
Long Quan Shao ◽  
Yuan Fu Yi ◽  
Jie Liu ◽  
...  
Keyword(s):  
Oral Mucosa ◽  
Skin Irritation ◽  
Inflammatory Cells ◽  
Skin Inflammation ◽  
Intradermal Injection ◽  
Cheek Pouch ◽  
Glass Composite ◽  
Skin Sensitivity ◽  
Biological Safety ◽  
Irritation Test

Objective: to describe and demonstrate an oral mucosa irritation and a skin sensitivity test for evaluation of the biological safety of a colored alumina/glass composite (AGC). Methods: colored alumina/glass composite and control materials were sutured to mucine bilateral cheek pouch mucosa, in a position for gross observation and for histological and cytological examination; skin irritation activity of the composite was tested by intradermal injection of a guinea pig test solution, by inducing, enhancing, and stimulating the allergic response observed after local tissue exposure. Results: colored AGC in mouse cheek pouch mucosa stimulated the same reaction as the control, with no significant inflammatory reaction or infiltration of inflammatory cells. Guinea pigs showed no obvious erythema or edema from the irritation test, such that the assessment of colored AGC skin allergy response in mice was 0%. Conclusion: colored AGC was benign towards mouse oral mucosa and elicited no allergic skin inflammation response, indicating that in these aspects this material had good biological safety.

A New Type of Colored Alumina/Glass Composite Biological Safety Assessment – Cell Toxicity and Hemolysis Tests

2010 ◽  
Vol 177 ◽  
pp. 459-461
Author(s):  
Bin Deng ◽  
Huai Xiu Lu ◽  
Long Quan Shao ◽  
Yuan Fu Yi ◽  
Jie Liu ◽  
...  
Keyword(s):  
Safety Assessment ◽  
Test Material ◽  
Blue Dye ◽  
Cell Toxicity ◽  
Glass Composite ◽  
Filtration Method ◽  
Biological Safety ◽  
New Type ◽  
Low Cytotoxicity ◽  
Control Samples

Objective: to evaluate the biological safety of a colored alumina/glass composite in terms of cytotoxicity and hemolytic ability. Methods: the cytotoxicity of an alumina/glass composite was evaluated with molecular filtration method. Cytotoxicity by spectrophotometric responses of test material and responses of a fresh rabbit red blood anticoagulant solution was determined by spectrophotometer. Results: cell-coated filter paper in contact with the test material showed the appearance of intracellular blue dye as the same staining density as in control samples, leading to a toxicity rating of 0. Colored alumina/glass composite assayed at <5% hemolysis. Conclusion: colored alumina/glass composite materials showed low cytotoxicity and non-acute hemolytic rates, representing overall good biological safety under these conditions.

scholarly journals STUDIES ON THE EFFECT OF TRITON (WR-1339) ON GUINEA PIG TISSUES

1958 ◽  
Vol 107 (1) ◽  
pp. 55-61 ◽  
Author(s):  
Robert A. Patnode ◽  
Paul C. Hudgins
Keyword(s):  
Guinea Pig ◽  
Guinea Pigs ◽  
Concentration Level ◽  
Intradermal Injection ◽  
Skin Sensitivity ◽  
Lytic Effect ◽  
Triton Wr 1339

The administration of triton WR-1339 (300 mg./kg. subcutaneously) to tuberculin-sensitive guinea pigs 2 hours before the intradermal injection of PPD depresses slightly their skin sensitivity to tuberculin and essentially obliterates the lytic effect of tuberculin on their circulating leucocytes. When leucocytes from tuberculin-sensitive guinea pigs are exposed to triton in vitro at a concentration level attainable in vivo the cells are partially protected against lysis by PPD.

scholarly journals Intradermal injection of low dose human regulatory T cells inhibits skin inflammation in a humanized mouse model

2018 ◽  
Vol 8 (1) ◽  
Author(s):  
Sija Landman ◽  
Vivian L. de Oliveira ◽  
Piet E. J. van Erp ◽  
Esther Fasse ◽  
Stijn C. G. Bauland ◽  
...  
Keyword(s):  
T Cells ◽  
Regulatory T Cells ◽  
Mouse Model ◽  
Low Dose ◽  
Skin Inflammation ◽  
Intradermal Injection ◽  
Humanized Mouse ◽  
Humanized Mouse Model

Correlation between evans blue dye and radiolabeled albumin in guinea pig airways in vivo

1989 ◽  
Vol 21 (4) ◽  
pp. 309-315 ◽  
Author(s):  
Duncan F. Rogers ◽  
Piera Boschetto ◽  
Peter J. Barnes
Keyword(s):  
Guinea Pig ◽  
Evans Blue ◽  
Blue Dye ◽  
Evans Blue Dye

scholarly journals Angioedema induced by a peptide derived from complement component C2.

1988 ◽  
Vol 168 (5) ◽  
pp. 1685-1698 ◽  
Author(s):  
C J Strang ◽  
S Cholin ◽  
J Spragg ◽  
A E Davis ◽  
E E Schneeberger ◽  
...  
Keyword(s):  
Guinea Pig ◽  
Vascular Permeability ◽  
Synthetic Peptides ◽  
Intradermal Injection ◽  
Amino Acid Residues ◽  
Terminal Sequence ◽  
Small Peptides ◽  
Rat Uterus ◽  
Pig Skin ◽  
Antihistaminic Drugs

Synthetic peptides that correspond to the COOH-terminal portion of C2b enhance vascular permeability in human and guinea pig skin. In human studies, 1 nmol of the most active peptide of 25-amino acid residues produced substantial local edema. A pentapeptide and a heptapeptide corresponding to the COOH-terminal sequence of C2b each induced contraction of estrous rat uterus in the micromole range; a peptide of 25 amino acids from this region induced a like contraction of rat uterus at a concentration 20-fold lower than the smaller peptides. The vascular permeability of guinea pig skin was enhanced by doses of these synthetic peptides in a similar fashion as that observed for the concentration of rat uterus. The induction of localized edema by intradermal injection in both the guinea pig and the human proceeds in the presence of antihistaminic drugs, suggesting that there is a histamine-independent component to the observed increase in vascular permeability. Cleavage of C2 with the enzymic subcomponent of C1, C1s, yields only C2a and C2b, and no small peptides, whereas cleavage of C2 with C1s and plasmin yields a set of small peptides. These plasmin-cleaved peptides are derived from the COOH terminus of C2b, and they induce the contraction of estrous rat uterus.

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