scholarly journals Evaluaton of the Use of Bone Implants as a Therapy for Deep Defects in the Parodoncium

2014 ◽  
Vol 15 (2) ◽  
pp. 79-82
Author(s):  
Momir Stevanović ◽  
Dušica Ćirić
Keyword(s):  
Tricalcium Phosphate ◽  
Periodontal Regeneration ◽  
Clinical Problem ◽  
Periodontal Pocket ◽  
Control Group ◽  
Lower Jaw ◽  
Standard Application ◽  
Inflammatory Processes ◽  
Infrabony Defects ◽  
And Control

ABSTRACT Reconstruction of infrabony defects created by the chronic inflammatory processes of parodontopathy represents a major clinical problem in paradontology. The objective of this research was to compare and evaluate the efficiency of two bone substituents at reversing the deep infrabony defects in the paradentium after the application of a new “Biohapel” material consisting of biphasic calcium phosphate/poly DL-lactide-co-glycolide. This study included 12 patients of both genders with an average age of 49.7 years who were suffering from clinically manifested parodontopathy. The main criteria for selecting patients were the presence of six similar infrabony periodontal defects that were at least 4 mm deep and in the lower side of the teeth on both sides of the jaw. After applying a basic causal parodontopathy treatment, defects were divided into two groups, including an experimental group (n=72) in which defects were reconstructed using the “Biohapel” bone substituent (on one side of the lower jaw) and a control group (n=72) in which infrabony defects were reconstructed using β tricalcium phosphate (βTCP) (CerasorbR), a preparation very commonly applied in regenerative parodontopathy treatment (on the other side of the lower jaw). Markers to assess treatment efficacy were determined before surgery and 6 months after surgery by measuring the depth of periodontal pocket and the level of the junctional epithelium. We observed statistically significant differences in the periodontal regeneration markers between the experimental and control groups (p<0.05). Application of “Biohapel” during the surgical treatment of infrabony defects in the paradentium significantly improves the bone regeneration of the paradentium when compared with the standard application of β-tricalcium phosphate .

scholarly journals Histopathological evaluation of polycaprolactone nanocomposite compared with tricalcium phosphate in bone healing

2018 ◽  
Vol 62 (3) ◽  
pp. 385-394 ◽  
Author(s):  
Hadi Eftekhari ◽  
Alireza Jahandideh ◽  
Ahmad Asghari ◽  
Abolfazl Akbarzadeh ◽  
Saeed Hesaraki
Keyword(s):  
Bone Tissue ◽  
Bone Healing ◽  
Tricalcium Phosphate ◽  
Healing Process ◽  
Control Group ◽  
Time Intervals ◽  
Positive Effects ◽  
Bone Scaffolds ◽  
Modern Engineering ◽  
And Control

AbstractIntroductionIn recent years, the use of bone scaffolds as bone tissue substitutes, especially the use of such as hydroxyapatite and tricalcium phosphate, has been very popular. Today, the use of modern engineering techniques and advances in nanotechnology have expanded the use of nanomaterials as bone scaffolds for bone tissue applications.Material and MethodsThis study was performed on 60 adult male New Zealand rabbits divided into four experimental groups: the control group without any treatment, the second group receiving hydroxyapatite, the third group treated with β-tricalcium phosphate, and the fourth group receiving nanocomposite polycaprolactone (PCL) scaffold. In a surgical procedure, a defect 6 mm in diameter was made in a hind limb femur. Four indexes were used to assess histopathology, which were union index, spongiosa index, cortex index, and bone marrow.ResultsThe results showed that nanocomposite PCL and control groups always had the respective highest and lowest values among all the groups at all time intervals. The histopathological assessment demonstrated that the quantity of newly formed lamellar bone in the nanocomposite PCL group was higher than in other groups.ConclusionAll these data suggest that PCL had positive effects on the bone healing process, which could have great potential in tissue engineering and clinical applications.

Change of Biomechanical Parameters in the Lower Jaws of Rabbits with Experimental Osteoporosis after Implantation of Calcium-Phosphate Bioceramic Material in the Greater Trochanter Region

2016 ◽  
Vol 721 ◽  
pp. 224-228
Author(s):  
Girts Salms ◽  
Vladislavs Ananjevs ◽  
Vladimirs Kasyanovs ◽  
Andrejs Skagers ◽  
Ilze Salma ◽  
...  
Keyword(s):  
Bone Tissue ◽  
Tricalcium Phosphate ◽  
Strontium Ranelate ◽  
Biomechanical Properties ◽  
Control Group ◽  
Greater Trochanter ◽  
Lower Jaw ◽  
Experimental Osteoporosis ◽  
Rabbit Bone ◽  
Biomechanical Parameters

Investigation of biomechanical properties of the rabbit bone tissue from a corner of the lower jaw was done. Experimental osteoporosis was induced by ovariectomy and following injections of methylprednisolone. The defects in the greater trochanter region was created and filled with granules of a hydroxyapatite and tricalcium phosphate (HAP/TCP 30/70) or HAP/TCP 30/70 together with 5% strontium ranelate. After 3 month animals were euthanased, squared samples have been cut out from a corner of the lower jaw and tested on a bend. Results of research show, that the corner of a lower jaw in rabbit becomes more rigid after filling of defects in the greater trochanter region with granules of a hydroxyapatite and tricalcium phosphate (HAP/TCP 30/70) or granules together with strontium ranelate. The ultimate strain for the bone tissue in the 2nd and 3rd group is less, than for control group. Thus, local uses calcium – phosphatic bioceramic material around the greater trochanter region improves biomechanical parameters of a bone tissue in the lower jaw of animals.

scholarly journals Experimental effect of collagen-containing medication on skin morphology

2017 ◽  
Vol 98 (6) ◽  
pp. 962-967 ◽  
Author(s):  
B N Sel’skaya ◽  
L A Musina ◽  
F Kh Kamilov
Keyword(s):  
Mature Female ◽  
Intradermal Injection ◽  
Female Rats ◽  
Control Group ◽  
Typical Structure ◽  
Injection Zone ◽  
Inflammatory Processes ◽  
Skin Morphology ◽  
And Control ◽  
Stimulation Of

Aim. To evaluate morphological skin changes of the experimental animals after intradermal injection of collagenous medication. Methods. The histological and histochemical methods (staining with hematoxylin and eosin, by Van-Gieson, Mallory and Foot method) were used to study the skin of mature female rats in the area of intradermal injection of collagen preparation «Kollost» by using mesotherapy method in 2, 4, 21 and 37 days. Murine skin was compared between experimental and control groups, in which the rats had solution of dextrose (glucose) injected intradermally. Results. On days 2-4 after the injection, inflammatory reaction was weak in the form of cellular infiltration along the needle stick. Numerous macrophages resorbing fibrous elements of the medication were determined in the injection zone. On days 7-14 fibroblasts proliferation, occurrence of argyrophilic thin newly-formed collagenous fibres and significant content of glycosaminoglycanes in the granulation tissue were revealed. By the 21-st day of the experiment following the injection, the dermal plate of the skin had become more dense due to the formation of thicker collagenous bundles in regeneration zones. When impregnated by silver nitrate they became yellow-brown that was indicative of fibre maturity. On day 37, the collagen fibers of the injected preparation in the injection zone were not detected in free unsubstituted form. The skin had a typical structure. The signs of stimulation of regeneration processes were not revealed in the skin of rats from the control group following the glucose solution injection. Conclusion. The collagenous preparation did not cause any pronounced inflammatory processes in the skin following intradermal injection to mature female rats; the fibrous structures of «Kollost» are resorbed by macrophages and substituted by the collagenous fibres integrating into the tissues; the processes are accompanied by stimulation of proliferation of structural elements of the skin connective tissue.

scholarly journals Comparative Study on the Application of Mesenchymal Stromal Cells Combined with Tricalcium Phosphate Scaffold into Femoral Bone Defects

2018 ◽  
Vol 27 (10) ◽  
pp. 1459-1468 ◽  
Author(s):  
Pavel Šponer ◽  
Tomáš Kučera ◽  
Jindra Brtková ◽  
Karel Urban ◽  
Zuzana Kočí ◽  
...  
Keyword(s):  
Stromal Cells ◽  
Tricalcium Phosphate ◽  
Bone Defects ◽  
Control Group ◽  
Graft Material ◽  
Synthetic Graft ◽  
Group A ◽  
Group B ◽  
And Control

This prospective study sought to evaluate the healing quality of implanted ultraporous β-tricalcium phosphate sown with expanded autologous mesenchymal stromal cells (MSCs) into femoral defects during revision hip arthroplasty. A total of 37 osseous defects in 37 patients were treated and evaluated concerning bone regeneration. Nineteen subjects received β-tricalcium phosphate graft material serving as a carrier of expanded autologous MSCs (the trial group A), nine subjects received β-tricalcium phosphate graft material only (the study group B) and nine subjects received cancellous allografts only (the control group C). Clinical and radiographic evaluations were scheduled at 6 weeks, 3, 6, and 12 months post-operatively, and performed at the most recent visit as well. All observed complications were recorded during follow-up to assess the use of an ultraporous β-tricalcium phosphate synthetic graft material combined with expanded MSCs in bone defect repair. The resulting data from participants with accomplished follow-up were processed and statistically evaluated with a Freeman–Halton modification of the Fischer’s exact test, a P < 0.05 value was considered to be significant. Whereas no significant difference was observed between the trial group A with β-tricalcium phosphate synthetic graft material serving as a carrier of expanded autologous MSCs and control group C with cancellous impaction allografting in terms of the bone defect healing, significant differences were documented between the study group B with β-tricalcium phosphate graft material only and control group C. Regarding adverse effects, six serious events were recorded during the clinical trial with no causal relationship to the cell product. β-tricalcium phosphate synthetic graft material serving as a carrier of expanded autologous MSCs appears safe and promotes the healing of bone defects in a jeopardized and/or impaired microenvironment. This clinical trial was registered at the EU Clinical Trials Register before patient recruitment (Registration number: EudraCT number 2012-005599-33; Date of registration: 2013-02-04).

scholarly journals Prognostic effects of SuPAR and Neopterin Levels on Patients with Lung Cancer

Pteridines ◽  
2020 ◽  
Vol 31 (1) ◽  
pp. 136-141
Author(s):  
Selim Yalcin ◽  
Mehmet Emin Demir ◽  
Reyhan Ozturk ◽  
Aytün Şadan Kılınç ◽  
Hatice Suer ◽  
...  
Keyword(s):  
Lung Cancer ◽  
Cox Regression ◽  
Soluble Form ◽  
Cancer Case ◽  
Control Group ◽  
Case Group ◽  
Lung Cancers ◽  
Type Plasminogen Activator ◽  
Inflammatory Processes ◽  
And Control

AbstractBackground: Two unique biomarkers, soluble form of the urokinase-type plasminogen activator receptor (suPAR) and neopterin, play a crucial role in inflammatory processes. This study aimed to reveal whether it is possible to utilize these biomarkers in predicting tumor prognosis in patients with lung cancers.Methods: The present study was designed as a single center, prospective, and controlled research. The study was conducted with forty patients with lung cancer (case group) and 41 healthy individuals (control group) in Kırıkkale University, Faculty of Medicine between 2016-2020. The case group was also divided into two of the early and advanced stages. The blood samples were drawn to evaluate suPAR and neopterin levels, and these parameters were compared between the case and control groups. Also, the prognostic effects of age, stage of the tumor, and the levels of mentioned parameters were investigated with the survival analysis.Results: The median duration of the follow-up was 32 (4-75) months. suPAR and neopterin levels were found to be higher in the case group than in the control group. Cox regression showed that the high levels of neopterin and suPAR increased mortality risk [p=0.002, HR: 1.25 (1.08-1.45 95%CI) and p=0.023, HR:1.07 (1.01-1.13), respectively]. Finally, age and stage of the tumor were found to have no relationship with survival.Conclusion: suPAR and neopterin as members of the inflammatory pathway were found to be higher in cancer cases. Furthermore, both suPAR and neopterin levels were found to be predictive for the mortality of patients with lung cancers; therefore, they are thought to be used for the management of cancer.

scholarly journals Nasal and Fecal Microbiota and Immunoprofiling of Infants With and Without RSV Bronchiolitis

2021 ◽  
Vol 12 ◽  
Author(s):  
Claudio Alba ◽  
Marina Aparicio ◽  
Felipe González-Martínez ◽  
María Isabel González-Sánchez ◽  
Jimena Pérez-Moreno ◽  
...  
Keyword(s):  
Fecal Microbiota ◽  
Control Group ◽  
High Concentration ◽  
Fecal Samples ◽  
Bacterial Microbiota ◽  
Healthy Infants ◽  
Rdna Sequencing ◽  
Inflammatory Processes ◽  
Syncytial Virus ◽  
And Control

Bronchiolitis associated with the respiratory syncytial virus (RSV) is the leading cause of hospitalization among infants aged &lt; 1 year. The main objective of this work was to assess the nasal and fecal microbiota and immune profiles in infants with RSV bronchiolitis, and to compare them with those of healthy infants. For this purpose, a total of 58 infants with RSV-positive bronchiolitis and 17 healthy infants (aged &lt; 18 months) were recruited in this case-control study, which was approved by the Ethics Committee of the Hospital Gregorio Marañón. Nasal and fecal samples were obtained and submitted to bacterial microbiota analysis by 16S rDNA sequencing and to analysis of several immune factors related to inflammatory processes. Nasal samples in which Haemophilus and/or Moraxella accounted for &gt; 20% of the total sequences were exclusively detected among infants of the bronchiolitis group. In this group, the relative abundances of Staphylococcus and Corynebacterium were significantly lower than in nasal samples from the control group while the opposite was observed for those of Haemophilus and Mannheimia. Fecal bacterial microbiota of infants with bronchiolitis was similar to that of healthy infants. Significant differences were obtained between bronchiolitis and control groups for both the frequency of detection and concentration of BAFF/TNFSF13B and sTNF.R1 in nasal samples. The concentration of BAFF/TNFSF13B was also significantly higher in fecal samples from the bronchiolitis group. In conclusion, signatures of RSV-associated bronchiolitis have been found in this study, including dominance of Haemophilus and a high concentration of BAFF/TNFSF13B, IL-8 and sTNF.R1 in nasal samples, and a high fecal concentration of BAFF/TNFSF13B.

scholarly journals “Air Polishing and CHX Effects in Addition to Subgingival Ultrasonic Instrumentation on Clinical Periodontal Parameters: A Randomised Clinical Trial”

2020 ◽  
Vol 6 (6) ◽  
Author(s):  
Ayse Caygur
Keyword(s):  
Randomised Clinical Trial ◽  
Periodontal Pocket ◽  
Control Group ◽  
Distilled Water ◽  
Beneficial Effects ◽  
Air Polishing ◽  
Probing Depth ◽  
Ultrasonic Devices ◽  
Hand Instrumentation ◽  
And Control

Objectives:  This study was performed to investigate the efficacy of using glycine powder air-polishing (GPAP) or  antiseptics (CHX) adjunctively to scaling and  root planing (SRP) in the treatment of periodontitis. Methods: In this trial, ninety patients (between 28-80 years old) who had at least three teeth with 3 to 7- mm periodontal pockets, were ıncluded from the Department of Periodontology. The patients were divided into three groups randomly. In the control group ultrasonic instrumentation was performed with distilled water and hand instrumentation, in the CHX group ultrasonic instrumentation was performed with CHX and hand instrumentation, in the GPAP group in addition to SRP with ultrasonic performed with distilled water and hand instrumentation, GPAP was performed for 10 seconds per periodontal pocket using a Perio-Flow device.  All treatments were performed in one session.  Resuls: The scores of  plaque index(PI),gingival index (GI), probing depth (PD), bleeding on probing (BOP) and clinical attachement level (CAL) were decreased in all groups after 1 month and the results were statistically significant in intergoup comparisions. PI and CAL scores were statistically significant in intragroup comparisions in GPAP group. Altough GI, PD and BOP scores were lower in the GPAP group than CHX and control group, the differences after 1 month were not statistically significant.  Conclusion: Within the limits of this study, it was seen that SRP was effective alone in the treatment of periodontitis. However, using a CHX with ultrasonic devices has little beneficial effects on periodontal parameters when compared with control and GPAP groups. GPAP groups was found more effectively in PI and CAL scores.

Bio-Engineered Mesenchymal Stem Cell-Tricalcium Phosphate Ceramics Composite Augmented Bone Regeneration in Posterior Spinal Fusion

2007 ◽  
Vol 334-335 ◽  
pp. 1201-1204 ◽  
Author(s):  
Chan Wai Chan ◽  
Ling Qin ◽  
K.M. Lee ◽  
H.Y. Yeung ◽  
Yun Yu Hu ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Spinal Fusion ◽  
Tricalcium Phosphate ◽  
Posterior Spinal Fusion ◽  
Control Group ◽  
Osteogenic Cells ◽  
Solid Fusion ◽  
Spinal Segments ◽  
And Control

Grafting of autologous iliac crest and decortication approach in posterior spinal fusion surgery has been the “gold standard”. However, the limited source of autograft has prompted extensive research into bone substitute and biological enhancement of the fusion mass. In this study, the application of stem cell therapy by tissue engineering method was investigated to enhance posterior spinal fusion with -tricalcium phosphate ceramics in rabbit model. Rabbit bone marrow derived mesenchymal stem cells were aspirated from trochanter region of proximal femur. The mesenchymal stem cells were grown and directed to differentiate into osteogenic cells by osteogenic supplement (ascorbic acid, -glycerophosphate and dexamethasone) in basal medium (10% FBS in DMEM). The osteogenic cells were seeded on tricalcium phosphate ceramics for one day (MSC group, n=6). The cell-ceramics composite was implanted onto autologous L5 and L6 transverse processes with decortication approach in posterior spinal fusion. The cell free ceramics acts as control (Control group, n=6) and iliac crest autograft as positive control (Autograft group). The spinal segments were harvested at week 7 post-operation. Manual palpation was performed with spinal segments to assess any movement of L5-L6 vertebral joint. The stiffness of the joint was considered as solid fusion. The specimens then were fixed by formalin and transferred to 70% ethanol. The BMC and volume of fusion transverse processes of L5 and L6 was measured by peripheral quantitative computed tomography. In manual palpation, 50% solid fusion was found in MSC group, 60% in autograft group but none in control group. Moreover, the BMC of L5 and L6 transverse processes in MSC group was greater than autograft and control group (45%, 40% respectively, p<0.01). The volume of transverse processes in MSC group was greater than autograft by 45% (p<0.01) and control group by 26% (p<0.05). In conclusion, the mesenchymal stem cells derived osteogenic cells augmented spinal fusion and bone mineralization.

scholarly journals Relationship between BMI ≥25 and periodontal status: A case‒ control study

2019 ◽  
Vol 10 (2) ◽  
pp. 90-94
Author(s):  
Seyed Ali Banihashemrad ◽  
Kazem Fatemi ◽  
Taher Pakdel ◽  
Nahid Nasrabadi
Keyword(s):  
Demographic Data ◽  
Normal Weight ◽  
Periodontal Pocket ◽  
Control Group ◽  
Case Group ◽  
Pocket Depth ◽  
Clinical Attachment Loss ◽  
Periodontal Status ◽  
Chi Squared ◽  
And Control

Background. Obesity is an important subject in both developed and developing countries. Obesity is a risk factor for many diseases, including cardiovascular diseases, hypertension and osteoarthritis. Periodontitis is a prevalent, chronic disease and multiple factors have been proposed to contribute to its progression. we aimed to compare the periodontal status of normalweight and obese individuals. Methods. In this study, we consecutively selected 100 patients (50 obese and overweight as the case group, based on body mass index [BMI], and 50 others with normal weight, as the control group) referred to the Periodontology Department of Mashhad Dental School. The demographic data of the participants were recorded, including age, gender, height and weight. The following periodontal parameters were assessed: periodontal pocket depth (PPD), clinical attachment level (CAL) and plaque index. Kolmogorov-Smirnov test, chi-squared test and independent t-test, as well as ANCOVA, were used to analyze data. Results. We found that the mean PPD was similar in the test and control groups (P=0.168). Moreover, CAL was not significantly different between the two groups (P=0.494). Conclusion. Our findings indicated that obesity and overweight do not seem to have an association with periodontal parameters such as periodontal pocket depth and clinical attachment loss. Further research is needed to evaluate this relationship.

scholarly journals The Morphology of Cross-Beaks and BMP4 Gene Expression in Huiyang Bearded Chickens

Animals ◽  
2019 ◽  
Vol 9 (12) ◽  
pp. 1143
Author(s):  
Yuyu Hong ◽  
Yuchang Pang ◽  
Haiquan Zhao ◽  
Siyu Chen ◽  
Shuwen Tan ◽  
...  
Keyword(s):  
Genetic Basis ◽  
Control Group ◽  
Jaw Bones ◽  
Lower Jaw ◽  
Morphogenetic Protein ◽  
Parietal Bones ◽  
And Control ◽  
The Impact ◽  
The Relationship

Bird beaks are important for biological purposes such as food intake, removing parasites, and defining phenotypic attributes. Cross-beaks are a threat to poultry health and are harmful to productivity, wasting some units in the poultry industry. However, there is still limited research on subtypes of cross-beaks and the genetic basis of cross-beaks as well. Here, we described the subtypes of cross-beaks in terms of left or right and upper or lower jaw bones. We evaluated the impact of cross-beaks on craniofacial bones and figured out the relationship between bone morphogenetic protein 4 (BMP4) and the development of craniofacial bones in Huiyang bearded chickens. We identified five typical subtypes of cross-beaks by morphological assessment and X-ray scanning. We found that cross-beaks caused certain changes in the facial bone morphology, including changes to the length and width of the bone around the ocular area (p < 0.05). The relative expressions of BMP4 in lacrimal, mandible, premaxilla, frontal, and parietal bones were significantly higher in the severe cross-beak group, followed by that of the medium cross-beak group, weak cross-beak group, and control group (p < 0.05). Overall, we constructed a generally applicable method to classify cross-beaks in term of the angle. The skeleton around the ocular area was affected by the cross-beak. The expression levels of BMP4 in craniofacial bones may provide insight to potential role of BMP4 in the development of cross-beaks.

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