Fabrication and Cytotoxicity Studies of the TiO2 Doped with Copper-Based Nanocomposite Particles

Since the growing interest in the manufacture and environmental applications of nanocomposites consisting of CuO and TiO2nanoparticles (NPs), related toxicological effect and interaction with cellular structures for these newly developed materials are still unknown. Recent literature reveals that nanosized CuO and TiO2particles have cytotoxicity risks and ability to cause oxidative stress on health. This work considers the CuO doped TiO2nanocomposite particles were synthesized via a coprecipitation method with aqueous solutions as precursors of copper nitrateand titanium dioxide. Moreover, the nanocomposite particles were characterized using TGA-DTA, UV-Vis and TEM measurements. The calcination temperature was selected at 873 K. The nanocomposite particles were characterized by TEM, as the primary particles, aggregates ranged from 30 to 100 nm and have a good dispersion character. Cell cytotoxicity assessment and the percentage cell survival was determined by using 3-(4,5-dimethylthiazol-2-yl)-5(3-carboxymethoxyphenol)-2-(4-sulfophenyl)-2H-tetrazoli um (MTS) assay on human fetal lung tissue cell (MRC-5). The experimental results show that the CuO doped TiO2nanocomposite particles cause potential cytotoxicity effect in cultured human cells.

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Study on the Properties and Cytotoxicity Assessment of Nanostructure Copper-Cerium Model Catalysts Prepared by Coprecipitation Approach

Advanced Materials Research ◽

10.4028/www.scientific.net/amr.160-162.1291 ◽

2010 ◽

Vol 160-162 ◽

pp. 1291-1296

Author(s):

Chang Mao Hung

Keyword(s):

Fetal Lung ◽

Copper Nitrate ◽

Ceo2 Nanoparticles ◽

High Dispersion ◽

Tissue Cell ◽

Particle Sizes ◽

Cultured Human Cells ◽

Cytotoxicity Assessment ◽

Human Fetal Lung ◽

Mts Assay

Since the growing interest in the manufacture and environmental applications of composites consisting of CuO and CeO2 nanoparticles. This work describes the CuO/CeO2 nanoparticle materials were synthesized by coprecipitation approach with aqueous solutions of copper nitrate and cerium nitrate. The nanocomposite particles were characterized by HRTEM, with a particle size around nanoscale particle sizes (~10 nm) with high dispersion phenomena. Further, cell cytotoxicity and the percentage cell survival was determined by using 3-(4,5-dimethylthiazol-2-yl)-5(3-carboxymethoxyphenol)-2-(4-sulfophenyl)-2H-tetrazoli um (MTS) assay on human fetal lung tissue cell (MRC-5). The experimental results show that the CuO/CeO2 nanoparticle materials only minor cause cytotoxicity effect in cultured human cells.

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Characterization and Cytotoxicity Studies of Mixed Cu-La-Ce Nanocomplexes Prepared by Coprecipitation Approach

Applied Mechanics and Materials ◽

10.4028/www.scientific.net/amm.110-116.527 ◽

2011 ◽

Vol 110-116 ◽

pp. 527-533

Author(s):

Chang Mao Hung

Keyword(s):

Fetal Lung ◽

Copper Nitrate ◽

High Dispersion ◽

Tissue Cell ◽

Particle Sizes ◽

Cell Cytotoxicity ◽

Cytotoxicity Studies ◽

Cultured Human Cells ◽

Human Fetal Lung ◽

Mts Assay

This work describes the mixed Cu-La-Ce nanocomplexes materials were synthesized by coprecipitation approach with aqueous solutions of copper nitrate, lanthanum nitrate and cerium nitrate. The nanocomplexes materials were characterized by TEM, with a particle size around nanoscale particle sizes (~50 nm) with high dispersion phenomena. Further, cell cytotoxicity and the percentage cell survival was determined by using 3-(4,5-dimethylthiazol-2-yl)-5 (3-carboxymethoxyphenol)-2-(4-sulfophenyl)-2H-tetrazolium (MTS) assay on human fetal lung tissue cell (MRC-5). The experimental results show that the Cu-La-Ce nanocomplexes materials only minor cause cytotoxicity effect in cultured human cells.

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The Influence of Calcination Temperature and Cytotoxicity Assessment of Honeycomb Platinum-Containing Cordierite Nanocomposite Catalysts via Incipient Wetness Impregnation Process

Applied Mechanics and Materials ◽

10.4028/www.scientific.net/amm.71-78.945 ◽

2011 ◽

Vol 71-78 ◽

pp. 945-949

Author(s):

Chang Mao Hung

Keyword(s):

Fetal Lung ◽

High Dispersion ◽

Tissue Cell ◽

Incipient Wetness Impregnation ◽

Impregnation Process ◽

Cultured Human Cells ◽

Incipient Wetness ◽

Nanocomposite Catalysts ◽

Cytotoxicity Assessment ◽

Ceramic Honeycomb

This work describes the platinum-containingcordieritenanocompositematerials were synthesized by incipient wetness impregnation process with aqueous solutions of H2PtCl6, Pd(NO3)3and Rh(NO3)3that were coated on cordierite ceramic honeycomb substances followed by calcinations temperature ranging from 973 to 1273 K. The nanocomposite materials were characterized by STEM, with a particle size around nanoscale particle sizes (~100 nm) with high dispersion phenomena. Further, cell cytotoxicity and the percentage cell survival was determined by using 3-(4,5-dimethylthiazol-2-yl)-5(3-carboxymethoxyphenol)-2-(4-sulfophenyl)-2H-tetrazoli um (MTS) assay on human fetal lung tissue cell (MRC-5). The experimental results show that the platrinum-containingcordieritenanocompositematerials only minor cause cytotoxicity effect in cultured human cells.

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Preparation, Electrochemical Properties and Cytotoxicity Assessment of Nanosized CuO/La2O3/CeO2 Composite for the Decomposition of Gaseous Ammonia

Materials Science Forum ◽

10.4028/www.scientific.net/msf.695.53 ◽

2011 ◽

Vol 695 ◽

pp. 53-56

Author(s):

Chang Mao Hung

Keyword(s):

Rare Earth ◽

Composite Materials ◽

Fixed Bed ◽

Fetal Lung ◽

Copper Nitrate ◽

Fixed Bed Reactor ◽

Gaseous Ammonia ◽

Tissue Cell ◽

Selective Catalytic Oxidation ◽

Cytotoxicity Assessment

This work considers the CuO/La2O3/CeO2nano-rare earth composite materials were synthesized by coprecipitation method with aqueous solutions of copper nitrate, lanthanum nitrate and cerium nitrate. The performance of the selective catalytic oxidation of ammonia to N2(NH3-SCO) over a CuO/La2O3/CeO2nano-rare earth composite materials in a tubular fixed-bed reactor (TFBR) at temperatures from 423 to 673 K in the presence of oxygen was reported. The catalytic redox behaviors were determined by cyclic voltammetry (CV). Further, cell cytotoxicity and the percentage cell survival were determined by using MTS assay on human fetal lung tissue cell (MRC-5). The experimental results show that the CuO/La2O3/CeO2nanocomposite particles only minor cause cytotoxicity effect in cultured human cells.

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Collagen secretion mediated by NMDR activation in human fetal lung fibroblasts

Cell Biology International ◽

10.1042/cbi034s070a ◽

2010 ◽

Vol 34 (8) ◽

pp. S70-S70

Author(s):

MingJie WANG ◽

ZiQiang LUO ◽

Mei LU ◽

LiHong SHANG ◽

ShaoJie YUE

Keyword(s):

Fetal Lung ◽

Lung Fibroblasts ◽

Collagen Secretion ◽

Human Fetal Lung

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Glucocorticoid regulation of epithelial sodium channel genes in human fetal lung

AJP Lung Cellular and Molecular Physiology ◽

10.1152/ajplung.1997.273.1.l227 ◽

1997 ◽

Vol 273 (1) ◽

pp. L227-L233 ◽

Cited By ~ 23

Author(s):

V. C. Venkatesh ◽

H. D. Katzberg

Keyword(s):

Rna Polymerase Ii ◽

Actinomycin D ◽

Critical Role ◽

Fetal Lung ◽

Explant Culture ◽

Late Gestation ◽

Na Channel ◽

Second Trimester ◽

Cyclic Monophosphate ◽

Human Fetal Lung

Pulmonary epithelial Na+ channels (ENaC), composed of three distinct subunits (alpha, beta, and gamma), play a critical role in the regulation of fluid reabsorption from airspaces of late-gestation fetal lung. We studied the expression of ENaC subunit genes in cultured human fetal lung. All three mRNAs were expressed at low levels in second trimester lung (13-32% of adult values at 24 wk gestation). There was a spontaneous increase of approximately threefold over preculture values of all three subunits within 24 h of explant culture in serum-free Waymouth's medium. Dexamethasone (Dex) induced all three mRNAs by two- to threefold. Maximal induction was noted by 8 h with 30-100 nM Dex and half-maximal stimulation with 3-10 nM Dex. Cycloheximide decreased basal expression of all three subunits by 8 h but did not alter the response to Dex. Actinomycin D and 5,6-dichloro-1-beta-D-ribofuranosylbenzimidazole (DRB), inhibitors of RNA polymerase II, decreased the basal and the Dex-induced expression of all three subunits with a more marked effect on human hENaC-gamma than on hENaC-alpha or hENaC-beta. Under conditions where transcription was blocked by actinomycin D or DRB, Dex did not alter the stability of the three mRNAs. Triiodothyronine (T3) at low (2 nM) or high (100 nM) concentrations had no effect on the expression of the three subunits in the presence or absence of low (10 nM) or high (100 nM) concentrations of Dex for 8 or 24 h. Similarly, 8-bromoadenosine 3',5'-cyclic monophosphate (2 microM) had no effect on basal or Dex-induced increase in the three subunits. We conclude that the three Na+ channel subunit genes are expressed in second trimester human fetal lung and are coordinately upregulated by glucocorticoid hormones but not by T3 or adenosine 3',5'-cyclic monophosphate. Glucocorticoid induction is receptor mediated, is primarily transcriptional, and does not require the induction of an intermediate protein for transcriptional enhancement. We speculate that induction of lung ENaC may contribute to the beneficial effects of antenatal glucocorticoids in premature babies.

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MORPHOGENETIC ASPECTS OF MULTILAYERING IN PETRI DISH CULTURES OF HUMAN FETAL LUNG FIBROBLASTS

The Journal of Cell Biology ◽

10.1083/jcb.41.1.298 ◽

1969 ◽

Vol 41 (1) ◽

pp. 298-311 ◽

Cited By ~ 42

Author(s):

Tom Elsdale ◽

Robert Foley

Keyword(s):

Extracellular Matrix ◽

Human Lung ◽

Petri Dish ◽

Fetal Lung ◽

Single Species ◽

Lung Fibroblasts ◽

Human Lung Fibroblasts ◽

Spontaneous Aggregation ◽

Control Hypothesis ◽

Human Fetal Lung

Randomly seeded Petri dish cultures of embryonic human lung fibroblasts generate, in the course of their growth, highly ordered cellular arrangements. Thick, bilaterally symmetrical ridges with an axial polarity and an orthogonal, multilayered internal organization are observed within stationary cultures. The generation of these structures has been investigated. Ridges result from the spontaneous aggregation of cells in postconfluent cultures brought about by directed cell movements. These movements are promoted by the localized production of extracellular matrix sheets containing collagen, which provide new substrates for cellular colonization. Cells that have colonized one matrix substrate may secrete another above themselves, which will in turn be colonized. By a continuation of this cycle, thick stacks consisting of alternate layers of cells and matrix are produced to yield the observed aggregations. The distribution and shape of ridges in a culture imply that matrix substrates are confined to specific locations. The suggested control hypothesis assumes that all the cells in fibroblast cultures are potential producers of a single species of matrix. The serviceability of this matrix as a substrate for cellular colonization, however, is destroyed if the producer cells are motile. Matrix substrates, therefore, are only made by nonmotile cells.

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Comparison of WI-38, MRC-5, and IMR-90 cell strains for isolation of viruses from clinical specimens

Journal of Clinical Microbiology ◽

10.1128/jcm.7.4.368-371.1978 ◽

1978 ◽

Vol 7 (4) ◽

pp. 368-371

Author(s):

H M Friedman ◽

C Koropchak

Keyword(s):

Tissue Culture ◽

Life Span ◽

Fetal Lung ◽

Cell Strain ◽

Viral Isolation ◽

Clinical Specimens ◽

Cell Strains ◽

Viral Diagnosis ◽

Human Fetal Lung ◽

Speed Of Onset

With the diminishing supply of the human fetal lung WI-38 cell strain, a replacement for viral isolation is needed. Two candidates are the human fetal lung strains MRC-5 and IMR-90. A comparison of WI-38, MRC-5, and IMR-90 was performed to evaluate efficiency and speed of viral isolation, clarity of cytophatic effect, and ease of growing the cells. The inocula were clinical specimens rather than tissue culture-adapted isolates. Frozen samples of 46 specimens that had previously yielded an isolate on WI-38 were thawed and inoculated onto WI-38, MRC-5, and IMR-90 cells. In addition, 95 freshly taken clinical specimens uf undetermined infectivity were inoculated onto the cell strains. Viral recovery rates were similar on all three strains, as were the appearance and speed of onset of the cytophatic effect. MRC-5 and WI-38 cells remained healthy until generation 36, whereas IMR-90 cells went into crisis by generation 20. The longer life span of the MRC-5 cells makes them more suitable than IMR-90 cells to replace the WI-38 strain for routine use in viral diagnosis.

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Oxygen modulates the differentiation of human fetal lung in vitro and its responsiveness to cAMP

AJP Lung Cellular and Molecular Physiology ◽

10.1152/ajplung.1993.264.5.l465 ◽

1993 ◽

Vol 264 (5) ◽

pp. L465-L474 ◽

Cited By ~ 13

Author(s):

M. J. Acarregui ◽

J. M. Snyder ◽

C. R. Mendelson

Keyword(s):

Gene Expression ◽

Atmospheric Oxygen ◽

Protein A ◽

Morphological Differentiation ◽

Fetal Lung ◽

Morphological Development ◽

Dependent Manner ◽

Concentration Dependent Manner ◽

Human Fetal Lung

Previously, it was found that lung explants from mid-trimester human abortuses differentiate spontaneously in organ culture in serum-free defined medium in an atmosphere of 95% air-5% CO2. Dibutyryl adenosine 3',5'-cyclic monophosphate (DBcAMP) treatment of human fetal lung in culture increases the rate of morphological differentiation and enhances expression of the surfactant protein A (SP-A) gene. To begin to define the factors responsible for this accelerated in vitro differentiation, we analyzed the effects of atmospheric oxygen on the morphological and biochemical development of human fetal lung in culture and on responsiveness of the cultured tissue to DBcAMP. We found that when lung explants were maintained in an atmosphere containing 1% oxygen they failed to differentiate spontaneously and no induction of SP-A gene expression was apparent. Furthermore, at 1% oxygen, DBcAMP had no effect to stimulate morphological differentiation or SP-A gene expression. When lung tissues that had been maintained for 5 days in 1% oxygen were transferred to an environment containing 20% oxygen, there was rapid morphological development and induction of SP-A gene expression. The effects on morphological development were manifest within 24 h of transfer to the 20% oxygen environment; within 72 h, a marked stimulatory effect of DBcAMP on SP-A gene expression also was observed. Our findings further suggest that the effects of oxygen on the levels of SP-A and SP-A mRNA are concentration dependent. Interestingly, the inductive effects of DBcAMP on SP-A gene expression were apparent only at oxygen concentrations > or = 10%. Morphological differentiation of the cultured human fetal lung tissue also was influenced by oxygen in a concentration-dependent manner. These findings suggest that oxygen plays an important permissive role in the spontaneous differentiation of human fetal lung in vitro.

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