Characterization of Fatty Acid Coated Polymer/Nucleotide Droplets

2016 ◽  
Vol 840 ◽  
pp. 213-219 ◽  
Author(s):  
Cik Rohaida Che Hak ◽  
Shogo Koga ◽  
T.Y.D. Tang ◽  
S. Mann
Keyword(s):  
Aqueous Solution ◽  
Fatty Acid ◽  
Origin Of Life ◽  
Self Assembly ◽  
External Environment ◽  
Biological Membranes ◽  
Living Matter ◽  
A Cell ◽  
Potential Alternative

The transition from non-living to living matter has been reported can be achieved in the laboratory via designing and constructing the protocell models [1]. Protocell is a study of origin of life theory whereby it is not a true cell, but a term used to refer to early cells or primitive cells. There are two types of protocell model; vesicles and coacervates. Vesicles are biological membranes; provide a semi-permeable boundary between the internal and external environment of a cell and are constructed via self-assembly of lipid and fatty acid molecules [2-5]. While vesicles are made of self-assembly of lipid molecules, coacervates are mixtures of two or more polymers in aqueous solution that spontaneously phase separated to form droplets.Coacervate droplets have been proposed as a potential alternative protocell model because of compartmentalization properties [6-11].

Characterization of pH-Responsive Self-Assembly Behaviors of Fatty Acid-Functionalized Prodrug

2020 ◽  
Vol 164 ◽  
pp. 107794
Author(s):  
Jin Han ◽  
Keita Hayashi ◽  
Yukihiro Okamoto ◽  
Keishi Suga ◽  
Hiroshi Umakoshi
Keyword(s):  
Fatty Acid ◽  
Self Assembly ◽  
Ph Responsive

scholarly journals On the Aqueous Solution Behavior of C-Substituted 3,1,2-Ruthenadicarbadodecaboranes

Inorganics ◽  
2019 ◽  
Vol 7 (7) ◽  
pp. 91 ◽  
Author(s):  
Marta Gozzi ◽  
Benedikt Schwarze ◽  
Peter Coburger ◽  
Evamarie Hey-Hawkins
Keyword(s):  
Aqueous Solution ◽  
Aqueous Solutions ◽  
Self Assembly ◽  
Self Assembling ◽  
Vis Spectroscopy ◽  
Assembly Behavior ◽  
Biological Performance ◽  
Type 3

3,1,2-Ruthenadicarbadodecaborane complexes bearing the [C2B9H11]2− (dicarbollide) ligand are robust scaffolds, with exceptional thermal and chemical stability. Our previous work has shown that these complexes possess promising anti-tumor activities in vitro, and tend to form aggregates (or self-assemblies) in aqueous solutions. Here, we report on the synthesis and characterization of four ruthenium(II) complexes of the type [3-(η6-arene)-1,2-R2-3,1,2-RuC2B9H9], bearing either non-polar (R = Me (2–4)) or polar (R = CO2Me (7)) substituents at the cluster carbon atoms. The behavior in aqueous solution of complexes 2, 7 and the parent unsubstituted [3-(η6-p-cymene)-3,1,2-RuC2B9H11] (8) was investigated via UV-Vis spectroscopy, mass spectrometry and nanoparticle tracking analysis (NTA). All complexes showed spontaneous formation of self-assemblies (108–109 particles mL−1), at low micromolar concentration, with high polydispersity. For perspective applications in medicine, there is thus a strong need for further characterization of the spontaneous self-assembly behavior in aqueous solutions for the class of neutral metallacarboranes, with the ultimate scope of finding the optimal conditions for exploiting this self-assembling behavior for improved biological performance.

Structural characterization of the model amphipathic peptide Ac-LKKLLKLLKKLLKL-NH2 in aqueous solution and with 2,2,2-trifluoroethanol and 1,1,1,3,3,3-hexafluoroisopropanol

2013 ◽  
Vol 91 (6) ◽  
pp. 406-413 ◽  
Author(s):  
Garry W. Buchko ◽  
Avijita Jain ◽  
Matthew L. Reback ◽  
Wendy J. Shaw
Keyword(s):  
Aqueous Solution ◽  
Self Assembly ◽  
Helical Structure ◽  
Cd Spectroscopy ◽  
Size Exclusion ◽  
Amphipathic Peptide ◽  
Amphipathic Peptides ◽  
Fluorinated Alcohols ◽  
Dilute Aqueous Solution

Short-chain amphipathic peptides are promising components in the new generation of engineered biomaterials. The model 14-residue leucine–lysine peptide Ac-LKKLLKLLKKLLKL-NH2 (LKα) is one such amphipathic peptide. In dilute aqueous solution (<0.05 mmol/L), it was previously proposed, using CD spectroscopic data, that LKα existed in a cooperative monomeric (unstructured) – tetrameric (α-helical) equilibrium that shifted towards the tetramer at high NaCl and peptide concentrations. Here, at similar peptide concentrations, CD spectroscopy shows that LKα readily adopts α-helical structure in the presence of 2,2,2-trifluoroethanol (TFE) and 1,1,1,3,3,3-hexafluoroisopropanol (HFIP) with maximal helical character in 20% TFE and ∼10% HFIP (v/v). The helical character in fluorinated alcohols suggested by the CD data at low peptide concentrations (0.06 mmol/L) is corroborated at high peptide concentrations (1.5 mmol/L) by NMR NOE data that also show that 1.5 mmol/L LKα is helical in 100% water. Size exclusion chromatography and estimations of rotational correlation times (τc) showed that the self-assembled LKα complexes contained three to five peptides. Removing the N-terminal acetyl group prevents LKα from forming helices and self-associating at high NaCl and peptide concentrations. This more detailed characterization of the structural and physical properties of LKα over a greater range of peptide concentrations and in the presence of fluorinated alcohols will assist the design of biomaterials containing amphipathic peptides and guide the ability to control self-assembly.

Self-assembly of well-defined fatty acid based amphiphilic thermoresponsive random copolymers

RSC Advances ◽  
2016 ◽  
Vol 6 (23) ◽  
pp. 19322-19330 ◽  
Author(s):  
Binoy Maiti ◽  
Sankar Maiti ◽  
Priyadarsi De
Keyword(s):  
Aqueous Solution ◽  
Fatty Acid ◽  
Stearic Acid ◽  
Self Assembly ◽  
Random Copolymers ◽  
Side Chain ◽  
Self Assembled

Side-chain stearic acid containing thermoresponsive and crystalline random copolymers are synthesized via RAFT technique, which self-assembled to spherical micellar structures in aqueous solution depending on stearate content in the copolymer.

Preparation and Characterization of Temperature-Sensitive 2-Hydroxy-3-Allyloxy Propyl Starch Ether

2015 ◽  
Vol 752-753 ◽  
pp. 81-85
Author(s):  
Ben Zhi Ju ◽  
Wei Ma ◽  
Hong Liang Yuan ◽  
Shu Fen Zhang
Keyword(s):  
Aqueous Solution ◽  
Fluorescence Spectroscopy ◽  
Self Assembly ◽  
Glycidyl Ether ◽  
Degree Of Substitution ◽  
Temperature Sensitive ◽  
H Nmr ◽  
Hydrophilic Lipophilic Balance ◽  
Allyl Glycidyl Ether

A temperature-sensitive 2-hydroxy-3-allyloxy-propyl starch ehter (HAPS) was prepared by regulating the hydrophilic-lipophilic balance of etherified starch. Allyl glycidyl ether (AGE) was used as the hydrophobic reagent. 1H-NMR was used to characterize the structure of products and determine the degree of substitution of etherified starch. UV-Vis and fluorescence spectroscopy methods were adopted to investigate the properties of HAPS aqueous solution. The results showed that the LCST of HAPS was reduced as the DS. When the cmc of HAPS decreases and DS increases, formation of micelles in an aqueous solution by self-assembly is possible.

scholarly journals Preparation and characterization of a porous material from an Algerian desert sand

2018 ◽  
Vol 5 (2) ◽  
pp. 48-56
Author(s):  
G. Mecheri ◽  
S. Hafsi ◽  
N. Gherraf
Keyword(s):  
Aqueous Solution ◽  
High Temperature ◽  
Porous Material ◽  
Self Assembly ◽  
Sol Gel ◽  
Desert Sand ◽  
Alkali Fusion ◽  
Sol Gel Process ◽  
Acid Aqueous Solution

Abstract The objective of the present work is to prepare and characterize a porous material using quite particular and localized dunar sand (erg) of the desert near the El-Oued (south-east of Algeria). The porous material is prepared according to the mechanism of co-operative self-assembly. The method consists of polymerizing a silicic precursor (sodium silicate obtained by alkali fusion of sand with the sodium carbonate) around micelles of surfactant in an acid aqueous solution according to the sol-gel process. The elimination of the surfactant by calcining at high temperature leads to the final material which was characterized by XRF, XRD, MEB-EDX, FTIR and BET techniques.

Preparation of BSA Nanoparticles by Desolvation Technique Using Acetone as Desolvating Agent

2012 ◽  
Vol 5 (1) ◽  
pp. 1643-1647
Author(s):  
Krishna Sailaja A ◽  
Amareshwar P
Keyword(s):  
Aqueous Solution ◽  
Standard Deviation ◽  
Process Parameters ◽  
Self Assembly ◽  
Size Control ◽  
Polymeric Materials ◽  
Preparation Condition ◽  
Assembly Process ◽  
Average Size

In order to see the functionality and toxicity of nanoparticles in various food and drug applications, it is important to establish procedures to prepare nanoparticles of a controlled size. Desolvation is a thermodynamically driven self-assembly process for polymeric materials. In this study, we prepared BSA nanoparticles using the desolvation technique using acetone as desolvating agent. Acetone was added intermittently into 1% BSA solution at different pH under stirring at 700 rpm. Amount of acetone added, intermittent timeline of acetone addition, and pH of solution were considered as process parameters to be optimized. The effect of the process parameters on size of the nanoparticles was studied. The results indicated that the size control of BSA nanoparticles was achieved by adding acetone intermittently. The standard deviation of average size of BSA nanoparticles at each preparation condition was minimized by adding acetone intermittently. The intermittent addition in polymeric aqueous solution can be useful for size control for food or drug applications.  

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