Preparation of BSA Nanoparticles by Desolvation Technique Using Acetone as Desolvating Agent

2012 ◽  
Vol 5 (1) ◽  
pp. 1643-1647
Author(s):  
Krishna Sailaja A ◽  
Amareshwar P
Keyword(s):  
Aqueous Solution ◽  
Standard Deviation ◽  
Process Parameters ◽  
Self Assembly ◽  
Size Control ◽  
Polymeric Materials ◽  
Preparation Condition ◽  
Assembly Process ◽  
Average Size

In order to see the functionality and toxicity of nanoparticles in various food and drug applications, it is important to establish procedures to prepare nanoparticles of a controlled size. Desolvation is a thermodynamically driven self-assembly process for polymeric materials. In this study, we prepared BSA nanoparticles using the desolvation technique using acetone as desolvating agent. Acetone was added intermittently into 1% BSA solution at different pH under stirring at 700 rpm. Amount of acetone added, intermittent timeline of acetone addition, and pH of solution were considered as process parameters to be optimized. The effect of the process parameters on size of the nanoparticles was studied. The results indicated that the size control of BSA nanoparticles was achieved by adding acetone intermittently. The standard deviation of average size of BSA nanoparticles at each preparation condition was minimized by adding acetone intermittently. The intermittent addition in polymeric aqueous solution can be useful for size control for food or drug applications.  

scholarly journals Self-Assembled Nanomaterials Based on Complementary Sn(IV) and Zn(II)-Porphyrins, and Their Photocatalytic Degradation for Rhodamine B Dye

Molecules ◽  
2021 ◽  
Vol 26 (12) ◽  
pp. 3598
Author(s):  
Nirmal K. Shee ◽  
Hee-Joon Kim
Keyword(s):  
Aqueous Solution ◽  
Visible Light Irradiation ◽  
Rhodamine B ◽  
Self Assembly ◽  
The Self ◽  
Assembly Process ◽  
Absorption Bands ◽  
Ligand Coordination ◽  
Rhodamine B Dye ◽  
Metal Ligand

A series of porphyrin triads (1–6), based on the reaction of trans-dihydroxo-[5,15-bis(3-pyridyl)-10,20-bis(phenyl)porphyrinato]tin(IV) (SnP) with six different phenoxy Zn(II)-porphyrins (ZnLn), was synthesized. The cooperative metal–ligand coordination of 3-pyridyl nitrogens in the SnP with the phenoxy Zn(II)-porphyrins, followed by the self-assembly process, leads to the formation of nanostructures. The red-shifts and remarkable broadening of the absorption bands in the UV–vis spectra for the triads in CHCl3 indicate that nanoaggregates may be produced in the self-assembly process of these triads. The emission intensities of the triads were also significantly reduced due to the aggregation. Microscopic analyses of the nanostructures of the triads reveal differences due to the different substituents on the axial Zn(II)-porphyrin moieties. All these nanomaterials exhibited efficient photocatalytic performances in the degradation of rhodamine B (RhB) dye under visible light irradiation, and the degradation efficiencies of RhB in aqueous solution were observed to be 72~95% within 4 h. In addition, the efficiency of the catalyst was not impaired, showing excellent recyclability even after being applied for the degradation of RhB in up to five cycles.

scholarly journals Robust and Repeatable Biofabrication of Bacteria-Mediated  Drug Delivery Systems: Effect of Conjugation Chemistry, Assembly Process  Parameters, and Nanoparticle Size

2021 ◽  
Author(s):  
Ying Zhan ◽  
Austin Fergusson ◽  
Lacey R. McNally ◽  
Richey M. Davis ◽  
Bahareh Behkam
Keyword(s):  
Drug Delivery ◽  
Growth Rate ◽  
Process Parameters ◽  
Self Assembly ◽  
Drug Delivery Systems ◽  
Delivery Systems ◽  
Dependent Manner ◽  
Assembly Process ◽  
Antitumor Effects ◽  
Therapeutic Modalities

Bacteria-mediated drug delivery systems comprising nanotherapeutics conjugated onto bacteria synergistically augment the efficacy of both therapeutic modalities in cancer therapy. Nanocarriers preserve therapeutics’ bioavailability and reduce systemic toxicity, while bacteria selectively colonize the cancerous tissue, impart intrinsic and immune-mediated antitumor effects, and propel nanotherapeutics interstitially. The optimal bacteria-nanoparticle (NP) conjugates would carry the maximal NP load with minimal motility speed hindrance for effective interstitial distribution. Furthermore, a well-defined and repeatable NP attachment density distribution is crucial to determining these biohybrid systems’ efficacious dosage and robust performance. Herein, we utilized our Nanoscale Bacteria-Enabled Autonomous Delivery System (NanoBEADS) platform to investigate the effects of assembly process parameters of mixing method, volume, and duration on NP attachment density and repeatability. We also evaluated the effect of linkage chemistry and NP size on NP attachment density, viability, growth rate, and motility of NanoBEADS. We show that the linkage chemistry impacts NP attachment density while the self-assembly process parameters affect the repeatability and, to a lesser extent, attachment density. Lastly, the attachment density affects NanoBEADS’ growth rate and motility in an NP size-dependent manner. These findings will contribute to the development of scalable and repeatable bacteria-nanoparticle biohybrids for applications in drug delivery and beyond. Corresponding author(s) Email:  [email protected]  

scholarly journals Structural conformation and self‐assembly process of p31‐43 gliadin peptide in aqueous solution. Implications for celiac disease

FEBS Journal ◽  
2019 ◽  
Vol 287 (10) ◽  
pp. 2134-2149 ◽  
Author(s):  
María Georgina Herrera ◽  
María Florencia Gómez Castro ◽  
Eduardo Prieto ◽  
Exequiel Barrera ◽  
Veronica Isabel Dodero ◽  
...  
Keyword(s):  
Aqueous Solution ◽  
Celiac Disease ◽  
Self Assembly ◽  
Assembly Process ◽  
Structural Conformation ◽  
Gliadin Peptide

Wide range (20–200 nm) size control of spherical ferrite particles grown on seed crystals in aqueous solution added with sucrose

2009 ◽  
Vol 24 (6) ◽  
pp. 2051-2055 ◽  
Author(s):  
Toshiyuki Tanaka ◽  
Hironori Nagai ◽  
Masaru Tada ◽  
Takashi Nakagawa ◽  
Adarsh Sandhu ◽  
...  
Keyword(s):  
Aqueous Solution ◽  
Standard Deviation ◽  
Size Control ◽  
Green Rust ◽  
Spherical Particles ◽  
Geometric Standard Deviation ◽  
Seed Crystals ◽  
Wide Range ◽  
Seed Growth ◽  
Spherical Growth

Spherical particles of ferrite (intermediate between Fe3O4 and γ-Fe2O3) were grown on seed crystals (∼9 nm) via the green rust route in an aqueous solution added with sucrose, which promotes spherical growth. By highly dispersing the seed crystals in an HNO3 solution, we could control the diameter of the particles over a wide range of 20–200 nm (geometric standard deviation: 1.1–1.4) by changing the amount of the seed crystals. At the beginning of the seed growth, clusters of the seed crystals were resolved into smaller clusters, each composed of a few seed crystals.

A turn-on phosphorescent sensor of Pb2+ in water by the formation of a coordination polymer

2019 ◽  
Vol 48 (12) ◽  
pp. 3815-3818 ◽  
Author(s):  
Marco Villa ◽  
Myriam Roy ◽  
Giacomo Bergamini ◽  
Marc Gingras ◽  
Paola Ceroni
Keyword(s):  
Aqueous Solution ◽  
Detection Limit ◽  
Coordination Polymer ◽  
Self Assembly ◽  
The Self ◽  
Assembly Process ◽  
Turn On ◽  
Carboxylic Groups

Persulfurated asterisks functionalized with six carboxylic groups form a strongly green phosphorescent coordination polymer upon addition of Pb2+ ions in aqueous solution. The self-assembly process is selective and reversible, enabling Pb2+ sensing with a detection limit of 6.0 × 10−7 M.

scholarly journals Robust and Repeatable Biofabrication of Bacteria-Mediated  Drug Delivery Systems: Effect of Conjugation Chemistry, Assembly Process  Parameters, and Nanoparticle Size

2021 ◽  
Author(s):  
Ying Zhan ◽  
Austin Fergusson ◽  
Lacey R. McNally ◽  
Richey M. Davis ◽  
Bahareh Behkam
Keyword(s):  
Drug Delivery ◽  
Growth Rate ◽  
Process Parameters ◽  
Self Assembly ◽  
Drug Delivery Systems ◽  
Delivery Systems ◽  
Dependent Manner ◽  
Assembly Process ◽  
Antitumor Effects ◽  
Therapeutic Modalities

Bacteria-mediated drug delivery systems comprising nanotherapeutics conjugated onto bacteria synergistically augment the efficacy of both therapeutic modalities in cancer therapy. Nanocarriers preserve therapeutics’ bioavailability and reduce systemic toxicity, while bacteria selectively colonize the cancerous tissue, impart intrinsic and immune-mediated antitumor effects, and propel nanotherapeutics interstitially. The optimal bacteria-nanoparticle (NP) conjugates would carry the maximal NP load with minimal motility speed hindrance for effective interstitial distribution. Furthermore, a well-defined and repeatable NP attachment density distribution is crucial to determining these biohybrid systems’ efficacious dosage and robust performance. Herein, we utilized our Nanoscale Bacteria-Enabled Autonomous Delivery System (NanoBEADS) platform to investigate the effects of assembly process parameters of mixing method, volume, and duration on NP attachment density and repeatability. We also evaluated the effect of linkage chemistry and NP size on NP attachment density, viability, growth rate, and motility of NanoBEADS. We show that the linkage chemistry impacts NP attachment density while the self-assembly process parameters affect the repeatability and, to a lesser extent, attachment density. Lastly, the attachment density affects NanoBEADS’ growth rate and motility in an NP size-dependent manner. These findings will contribute to the development of scalable and repeatable bacteria-nanoparticle biohybrids for applications in drug delivery and beyond. Corresponding author(s) Email:  [email protected]  

scholarly journals Rapid formation and real-time observation of micron-sized conjugated nanofibers with tunable lengths and widths in 20 minutes by living crystallization-driven self-assembly

2020 ◽  
Vol 11 (32) ◽  
pp. 8416-8424 ◽  
Author(s):  
Sanghee Yang ◽  
Tae-Lim Choi
Keyword(s):  
Block Copolymers ◽  
Real Time ◽  
Self Assembly ◽  
Size Control ◽  
Assembly Process ◽  
The Real ◽  
Rapid Formation ◽  
Time Observation ◽  
Real Time Observation

New fully conjugated block copolymers formed semiconducting 1D nanofibers with excellent structural and size control. The rapid living CDSA enabled us to watch the real-time video of the whole self-assembly process.

Self-assembly and anion sensing of metal–organic [M6L2] cages from fluorescent triphenylamine tri-pyrazoles with dipalladium(ii,ii) corners

2017 ◽  
Vol 46 (18) ◽  
pp. 5801-5805 ◽  
Author(s):  
Yu Cui ◽  
Zi-Man Chen ◽  
Xuan-Feng Jiang ◽  
Jin Tong ◽  
Shu-Yan Yu
Keyword(s):  
Aqueous Solution ◽  
Self Assembly ◽  
Assembly Process ◽  
Anion Sensing ◽  
Metal Organic

Three supramolecular metallo-cages are synthesized from the fluorescent triphenylamine functionalized tri-pyrazoles via a directed self-assembly process in aqueous solution.

scholarly journals Robust and Repeatable Biofabrication of Bacteria-Mediated  Drug Delivery Systems: Effect of Conjugation Chemistry, Assembly Process  Parameters, and Nanoparticle Size

2021 ◽  
Author(s):  
Ying Zhan ◽  
Austin Fergusson ◽  
Lacey R. McNally ◽  
Richey M. Davis ◽  
Bahareh Behkam
Keyword(s):  
Drug Delivery ◽  
Growth Rate ◽  
Process Parameters ◽  
Self Assembly ◽  
Drug Delivery Systems ◽  
Delivery Systems ◽  
Dependent Manner ◽  
Assembly Process ◽  
Antitumor Effects ◽  
Therapeutic Modalities

Bacteria-mediated drug delivery systems comprising nanotherapeutics conjugated onto bacteria synergistically augment the efficacy of both therapeutic modalities in cancer therapy. Nanocarriers preserve therapeutics’ bioavailability and reduce systemic toxicity, while bacteria selectively colonize the cancerous tissue, impart intrinsic and immune-mediated antitumor effects, and propel nanotherapeutics interstitially. The optimal bacteria-nanoparticle (NP) conjugates would carry the maximal NP load with minimal motility speed hindrance for effective interstitial distribution. Furthermore, a well-defined and repeatable NP attachment density distribution is crucial to determining these biohybrid systems’ efficacious dosage and robust performance. Herein, we utilized our Nanoscale Bacteria-Enabled Autonomous Delivery System (NanoBEADS) platform to investigate the effects of assembly process parameters of mixing method, volume, and duration on NP attachment density and repeatability. We also evaluated the effect of linkage chemistry and NP size on NP attachment density, viability, growth rate, and motility of NanoBEADS. We show that the linkage chemistry impacts NP attachment density while the self-assembly process parameters affect the repeatability and, to a lesser extent, attachment density. Lastly, the attachment density affects NanoBEADS’ growth rate and motility in an NP size-dependent manner. These findings will contribute to the development of scalable and repeatable bacteria-nanoparticle biohybrids for applications in drug delivery and beyond. Corresponding author(s) Email:  [email protected]  

Capillary flows and self-assembly of porous hydrate structures

2012 ◽  
Vol 9 (1) ◽  
pp. 22-25
Author(s):  
S.V. Amel’kin ◽  
D.Ye. Igoshin
Keyword(s):  
Experimental Data ◽  
Aqueous Solution ◽  
Self Assembly ◽  
Capillary Flow ◽  
Secondary Crystallization ◽  
Assembly Model ◽  
Physical Processes ◽  
Good Correspondence ◽  
Solution Formation ◽  
Capillary Flows

A self-assembly model for porous hydrate structures is proposed, which takes into account the sequence of basic physical processes: hydrate growth on the surface of the aqueous solution, formation of islet structure, capillary flow, separation and transfer of secondary crystallization nuclei to the meniscus. The model was studied within the cellular automata method. A good correspondence between the results of the simulation and the experimental data is obtained.

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