scholarly journals Wound Healing Effects of Piper nigrum L. and Coffea canephora in Rats

2019 ◽  
Vol 967 ◽  
pp. 9-14
Author(s):  
Hartati ◽  
Suryani Idris Irma ◽  
Dini Iwan ◽  
Rahmawati Nur ◽  
Ali Alimuddin
Keyword(s):  
Wound Healing ◽  
Ethyl Acetate ◽  
Topical Application ◽  
Coffea Canephora ◽  
Piper Nigrum ◽  
Excision Wound ◽  
Wound Healing Activity ◽  
Ethyl Acetate Extracts

The ethanolic and ethyl acetate extracts of Piper nigrum L and Coffea canephora were evaluated for their wound healing activity in rats. Wound healing activity was studied using excision wound models in rats following topical application. Animals were divided into eight groups with three in each group. Ten Percent w/v extract of each plant was prepared in gel CMC-Na for topical application. Piper nigrum L possesses better wound healing activity than Coffea canephora.

scholarly journals EVALUATION OF ANTIOXIDANT, ANTIMICROBIAL AND WOUND HEALING ACTIVITY OF POIKILOSPERMUM SUAVEOLENS

2021 ◽  
Vol 84 (1) ◽  
pp. 41-48
Author(s):  
Hartati Ahmad ◽  
Muhammad Habil Ahmad ◽  
Alimuddin Ali ◽  
Halifah Pagarra ◽  
Pince Salempa ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Antioxidant Activity ◽  
Wound Healing ◽  
Ethyl Acetate ◽  
Inhibition Zone ◽  
Positive Control ◽  
Skin Injury ◽  
Treatment Of Wounds ◽  
Wound Healing Activity ◽  
Ethyl Acetate Extracts

Poikilospermum suaveolens is a plant could grows in the limestone area in Maros, Indonesia. The leaves are generally widely used as traditional medicine for treatment of wounds by local communities. This study aims to examine the wound healing potential of P. suaveolens leaves extract as well as scientific clarify of the plant. The plant leaves were extracted by maceration. The measurement of antioxidant activity by the DPPH technique (1,1 diphenil-2-pikrilhidrazil) spectrophotometrically. The antimicrobial activity was evaluated by formation of the inhibition zone. The wound healing activity was performed the percentage of injury closure in rats skin injury. The antioxidant activity showed that 70% ethanol and ethyl acetate extracts were not significantly different, however, highly distinct to the positive control (Butylated Hidroxyanisole). The antimicrobial activity of extract showed 70% ethanol and ethyl acetate extracts were not significantly against Staphylococcus aureus and Candida albicans, but significantly against Pseudomonas aeruginosa. The evaluation of P. suaveolens extract on the rate of wound closure activity quickly, i.e., within 15 days the injury on the test animal was recovered. This study concludes that the P. suaveolens plant extracts are potential as a sources of active substances for wound healing.

COMPARATIVE EVALUATION OF IN VITRO ANTIMICROBIAL AND IN VIVO WOUND HEALING ACTIVITY OF ETHANOLIC AND ETHYL ACETATE EXTRACTS OF ABUTILON INDICUM ROOT

INDIAN DRUGS ◽  
2019 ◽  
Vol 56 (04) ◽  
pp. 21-31
Author(s):  
K Ravishankar ◽  
G.V.N Kiranmayi ◽  
J. M. Sruthi ◽  
Keyword(s):  
Antimicrobial Activity ◽  
Wound Healing ◽  
Ethyl Acetate ◽  
Ethanolic Extract ◽  
Diffusion Method ◽  
Wound Healing Activity ◽  
Ethyl Acetate Extracts ◽  
Abutilon Indicum

The present study was aimed at comparing the in vivo wound healing and in vitro antibacterial activity of Abutilon indicum roots. Wound healing is a complex phenomenon which consists of three stages inflammatory, proliferative, and remodelling. Traditionally, A. indicum is used for wound healing. There is no specific scientific data available for the wound healing activity of Abutilon indicum root. The present study was designed to investigate the same. The wound healing activity of ethanolic and ethyl acetate extracts of A. indicum was evaluated in incision and burn wound models. The parameters studied include tensile strength, epithelialisation period and rate of wound contraction. A. indicum also possesses antimicrobial activity. Ethyl acetate and ethanolic extract of A. indicum was tested against both Gram positive, Gram negative and fungal organisms using agar well diffusion method. From the results, it was concluded that ethyl acetate extract of A. indicum root had greater wound activity and antimicrobial activity than the ethanolic extract.

Wound Healing Activity of Ten Indigenous Plants in Incision and Excision Wound Models in Albino Rats

2012 ◽  
Vol 2 (9) ◽  
pp. 56-58
Author(s):  
Vasanthalaxmi K Vasanthalaxmi K ◽  
◽  
Saraswathi Udupa ◽  
Laximinarayana Udupa
Keyword(s):  
Wound Healing ◽  
Albino Rats ◽  
Indigenous Plants ◽  
Excision Wound ◽  
Wound Healing Activity

Comparison of the ficus for the wound healing activity in various species

2020 ◽  
Vol 10 (4) ◽  
pp. 67-70
Author(s):  
Mothilal K ◽  
Akila CR ◽  
Mahender K ◽  
Chaitanya Kumar K ◽  
Ravi D
Keyword(s):  
Wound Healing ◽  
Wound Closure ◽  
Subcutaneous Tissue ◽  
Significant Activity ◽  
Herbal Extracts ◽  
Standard Drug ◽  
Excision Wound ◽  
Injuries And Wounds ◽  
Skin Conditions ◽  
Wound Healing Activity

Injuries and wounds are any sorts of damage to the skin or subcutaneous tissue. Usually, any wounds of such sorts are self-healed. Sometimes, there may be a delay in healing, and that delay is caused due to the functional delays in various processes of wound healing. All the Ficus plants show similar activities like the antioxidant, anti-inflammatory and wound healing properties 7including skin conditions like ulcers and rheumatism. The anthelmintic property and immunomodulatory are also seen. The herbal extracts of the same family of Ficus in different plants were investigated for the wound healing activity in the excision wound method, and the extracts showed significant activity compared to the drug. All the extracts showed a better healing ability, but the extract of FBO-100 showed the highest activity followed by FMO followed by FHO and finally the FRO. Overall, the activity of the extract ointment was comparable and was significant compared to the standard drug ointment. The wound closure of the extract ointment treated groups were better and were completed in 12 days, and the activity was more than 96%. The herbal extracts of the same family of Ficus in different plants were investigated for the wound healing activity in the excision wound method, and the extracts showed significant activity compared to the drug. The plants of microcarpa, benghalensis, religiosa and hispida are compared for the activity, and the order showed for the activity was FBO>FMO>FHO>FRO.

WOUND HEALING ACTIVITY OF THE WHOLE PLANT OF MOMORDICA CHARANTIA LINN. IN RATS

INDIAN DRUGS ◽  
2018 ◽  
Vol 55 (11) ◽  
pp. 64-70
Author(s):  
M.P Singh ◽  
◽  
Y.S. Sarangdevot ◽  
S.S. Sisodia
Keyword(s):  
Wound Healing ◽  
Ethyl Acetate ◽  
Petroleum Ether ◽  
Ethanolic Extract ◽  
Momordica Charantia ◽  
Alcohol Extract ◽  
Whole Plant ◽  
Healing Property ◽  
Aqueous Ethanolic Extract ◽  
Wound Healing Activity

The aim of the present study was to explore the effect of wound healing activity of the whole plant of Momordica charantia Linn. in rats. The effect of aqueous ethanolic extract of Momordica charantia Linn. (Family; Cucurbetaceae) was evaluated on resutured incision, granuloma pouch and excision wound models in rats. The wound healing activity was measured by the rate of wound contraction, skin breaking strength, weight of the granulation tissue, and collagen content. The concentrated ethyl alcohol extract was subject to fractionation by using petroleum ether (40-60o V/V), solvent ether, ethyl acetate, butanol, butanone, ethanol and methanol in succession. All the fractions were screened for wound healing property. The results of pharmacological activity led to the conclusion that the petroleum ether fraction exhibited more significant wound healing promotion property followed by solvent ether and butanone, from all the parameters studied. Ethyl acetate, ethanol and methanol fractions were least effective.

scholarly journals EVALUATION OF WOUND HEALING ACTIVITY OF METHANOLIC EXTRACT OF SMILAX WIGHTII (A. DC.) IN WISTAR ALBINO RATS

2014 ◽  
Vol 1 (1) ◽  
pp. 73-77
Author(s):  
Uma Maheswari P ◽  
Shalimol A ◽  
Arumugasamy A ◽  
Udhaya Sankar M.R
Keyword(s):  
Wound Healing ◽  
Dry Weight ◽  
Wound Contraction ◽  
Albino Rats ◽  
Standard Drug ◽  
Excision Wound ◽  
Wound Model ◽  
Wistar Albino Rats ◽  
Incision Wound ◽  
Wound Healing Activity

The present experimental study was designed to evaluate the wound healing activity of methanolic extract of Smilax wightii A. DC. on incision and excision wound models in Wistar albino rats. The parameters studied were wound breaking strength, wound contraction area, epithelialization period, granulation tissuewet, dry weight and hydroxyproline content in incision wound model, percentage of wound contraction and period of epithelialization in excision wound model. The rats were administered topically with 100mg/kg b.wt. (low dosage), 200mg/kg b.wt. (moderate dosage) and 500mg/kg b.wt. (high dosage) of methanolicextract of Smilax wightii (MESW). The activity of the extract treated groups were compared with that of the control 1% Spirit. Framycetin sulphate 0.2% w/w was used as the standard drug. In incision wound model, there was a significant increase in the wound breaking strength in all the experimental groups treated withMESW than that of the control. Similarly, significant (P<0.001) decrease in wound contraction area and period of epithelialization were also observed in the test group animals treated with MESW and the standard drug treated groups when compared to that of the control. , a significant increase was observed in granulation tissue wet and dry weight and hydroxyproline content in the test groups treated with MESW compared to the control. In exicision wound model, there was a significant increase (P<0.01) in the percentage of wound contraction and decrease in period of epithelialization in the experimental groups treated with 200mg/kgb.wt. (moderate dosage) and 500mg/kgb.wt (high dosage) of MESW. The extract treated groups showed significant improvement in all the wound healing parameters of incision, and excision wound models.

scholarly journals Wound healing activity of Conyza canadensis (L.) Cronquist

2018 ◽  
Vol 1 (T5) ◽  
pp. 35-40
Author(s):  
To Dinh Le ◽  
Nga Thi Hoang ◽  
Nhan Thi Thanh Nguyen ◽  
Thuoc Linh Tran ◽  
Thao Thi Phuong Dang
Keyword(s):  
Wound Healing ◽  
Antibacterial Activity ◽  
Ethyl Acetate ◽  
National Park ◽  
Ethanolic Extract ◽  
Ethyl Acetate Fraction ◽  
Conyza Canadensis ◽  
Keratinocyte Proliferation ◽  
Wound Healing Activity ◽  
Stimulation Of

Conyza canadensis (L.) Cronquist (CCL) has been used by K’Ho minority in Bidoup Nui Ba national park, Lam Dong province, Vietnam as one of wound healing remedies. However, the scientific proof of treatment is still unclear. This study aimed to evaluate this wound healing potential of CCL. CCL power was extracted by ethanol and then partitioned consecutively with petroleum ether, ethyl acetate and water. Wound healing potential was evaluated by antibacterial activity, stimulation of fibroblast and keratinocyte proliferation. Agar-well diffusion was used in the antibacterial tests and the results showed that CCL had antibacterial activity against 02 dermatitis bacteria (Pseudomonas aeruginosa, Staphylococcus aureus) and 02 opportunistic infection bacteria (Escherichia coli, Enterococcus faecalis). Moreover, our results illustrated that CCL stimulated the fibroblast and keratinocyte proliferation compared to the control. Particularly, the fibroblast division increased 1.6 times at 31.25 µg/mL when treated by ethanolic extract, while ethyl acetate fraction showed 1.7 times increase at 10 µg/mL in keratinocyte proliferation compared to the control. Taken together, our study contributed scientific base of CCL in the wound healing.

scholarly journals Evaluation of Wound Healing Activity of an Ethanolic Extract of Anacardiumoccidentale Leaves in Wistar Rats

2020 ◽  
Vol 13 (4) ◽  
pp. 2061-2068
Author(s):  
Meena Kumari K ◽  
Amberkar Mohanbabu Vittalrao ◽  
Praveen Kumar SE ◽  
Sushma Prabhath ◽  
Charitha Charitha
Keyword(s):  
Wound Healing ◽  
Wistar Rats ◽  
Povidone Iodine ◽  
Histopathological Examination ◽  
Ethanolic Extract ◽  
Wound Contraction ◽  
Saline Control ◽  
Standard Drug ◽  
Excision Wound ◽  
Wound Healing Activity

BACKGROUND -This study undertaken to screen a potential wound healing activity of an ethanolic extract of Anacardiumoccidentale(Ao)leaves in Wistar rats. MATERIALS AND METHODS-Excision wound of 500mm2created on the back of Wistar rats. A total of thirty adult Wistar rats were prescreened and divided into five groups of six rats each.Group I and II rats were treated by normal saline (control) and povidone-iodine (standard drug), respectively. Group III and IV with 0.5% and 2%of Anacardiumoccidentale ointment (test drugs), respectively, and Group V rats treated with anti-wound healer drug dexamethasone, i.m. + 2% Anacardiumoccidentale. All drugs were applied topically except Inj. dexamethasone. In the model of excision wound, the wound healing effect was determined by parameters such as the percentage of wound contraction, duration of epithelialization, and histopathological examination. RESULT-All drugs, povidone-iodine, 0.5%, and 2% of the Ao ointment treated group of rats showed a statistically significant increase (P<0.05) in the % of wound contraction on 5th and 6thdays Vs.Control rats. In contrast,povidone-iodine, 2% Ao ointment and (dexamethasone + 2% Ao)treated rats decreased the period of epithelialization which was statistically significant (P<0.05) compared to the control.However, the standard povidone-iodine treated rats showed a maximum increase in the percentage of wound contraction and minimum period of epithelialization, followed by a higher dose of 2% Ao extract. The plant extract 0.5% and 2% of Ao showeda significant increase inthe rate of wound contraction as compared to the standard drug povidone-iodine also. The combination of (2%Aoextract+dexamethasone) drug-treated rats decreased the rate of wound contraction on 5th, 9th days; whereas, povidone-iodine increased on all days, i.e., 5th, 9th, 13th and 17th days,which was statistically significant (p<0.05) Vs.Normal saline. The povidone and 2% of Ao extract hastened the rate of wound healing activity, which was evident by maximum re-epithelialization and presence of the collagen fibers. CONCLUSION-In our study, the topical application of Anacardiumoccidentale leaf extract has shown promising wound healing activity; however, it failed to reverse the anti-wound healing property of steroids.

scholarly journals Effects of Methanolic Extract Based-Gel From Saudi Pomegranate Peels With Enhanced Healing Potential on Excision Wounds in Diabetic Rats

2021 ◽  
Vol 12 ◽  
Author(s):  
Shahid Karim ◽  
Huda M. Alkreathy ◽  
Aftab Ahmad ◽  
Mohammad Imran Khan
Keyword(s):  
Wound Healing ◽  
Growth Factor ◽  
Treatment Group ◽  
Vehicle Control ◽  
Post Treatment ◽  
Pomegranate Peel ◽  
Excision Wound ◽  
Pomegranate Peel Extract ◽  
Peel Extract ◽  
Wound Healing Activity

Introduction: Current study was designed to evaluate the wound healing activity of a Saudi pomegranate peel extract on excision wound healing in experimentally induced diabetes in rats.Methodology: Animals were divided into three groups: diabetic excision wound with no treatment, diabetic excision wound with gel alone and diabetic excision wound with Saudi pomegranate peel extract in gel. Animals were monitored for clinical signs, weekly body weight, morbidity and mortality during entire study period. The efficacy parameters evaluated were percent wound contraction, Hydroxyproline content, estimation of Transforming Growth Factor ß1 (TGF-ß1), Vascular Endothelial Growth Factor (VEGF), and Epidermal Growth Factor (EGF) in wound lysates by ELISA, mRNA expression of TGF-ß1, VEGF, and EGF in wound lysates by qPCR, Estimation of nitric oxide (NO) and NO synthase (NOS) in Wound Lysates and histopathology of skin for reepithelization, neovascularization, and inflammation.Results: The Saudi pomegranate peel extract in gel (5.0 g extract per 100 g gel) showed significant wound healing activity when compared to the vehicle control [p &lt; 0.05] following 21 days of treatment. Animals in the control and treatment groups were apparently normal through the study with no significant differences in body weights between groups. Expression of mRNA of TGFβ1, EGF and VEGF in wounds was the highest on day 14 post treatment 4.3, 3.5 and 0.9 fold higher respectively in the treatment group when compared to vehicle control, and on day 21, the values were 0.12, 0.3 and 0.83, respectively. No statistically significant differences were observed in TGF-ß1 levels in wounds on days 4, 7, 14 and 21 post treatment when compared to the vehicle control (p &gt; 0.05). Significantly higher levels of VEGF were observed in treatment group on day 7 and 21 when compared to vehicle control (p &lt; 0.05). Significantly higher levels of EGF were observed in treatment group on day 7 and 21 when compared to vehicle control (p &lt; 0.05). Mean hydroxyproline levels were higher in treatment group on days 4 and 7 when compared to vehicle control. NO levels in treatment group were significantly lower on days 7, 14 and 21 when compared to vehicle control (p &lt; 0.05). NOS activity in treatment group were significantly lower on days 4 and 7 when compared to vehicle control (p &lt; 0.05). Histopathological changes in skin wound in the treatment group were consistent with wound healing when compared to the vehicle group.Conclusion: This study’s findings suggest that topical application of SPPE gel effectively enhanced wound healing in experimentally induced diabetic conditions.

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