The Biological Effect of Iron Oxide and Its Hydrate Nanoparticles

2007 ◽  
Vol 121-123 ◽  
pp. 735-738 ◽  
Author(s):  
Qiang Zhao ◽  
X.F. Pang ◽  
Le Wei Liu ◽  
Bo Deng
Keyword(s):  
Amino Acids ◽  
Iron Oxide ◽  
Infrared Spectrum ◽  
Biological Effect ◽  
Crystalline Form ◽  
Diphenyltetrazolium Bromide ◽  
Pure Amino Acid ◽  
Space Effect ◽  
Low Toxicity ◽  
Crystalline Forms

Iron oxide and its hydrate nanoparticles were synthesized by hydrothermal method and confirmed by infrared and SEM (Scanning Electron Microscope) et al. The dimensions of the nanoparticles are about 50-120 nm. The crystalline form of iron oxide nanoparticles is like globosity while its hydrate rod. Amino acids intermingling with the synthesized nanoparticles were crystallized to investigate the space effect of the nanoparticles. The crystalline forms of crystal are different to that of pure amino acid. The positions and width of the nanoparticles’ peaks in the infrared spectrum are changed too. Microscope observation and infrared spectrum results indicated the nanoparticles had changed the internal structure of amino acids crystal. To considerate the toxicity of the synthesized nanoparticles, MTT (3-(4,5-dimethylthiazol 2-yl)-2,5 diphenyltetrazolium bromide) assay was used to determine their cytotoxicity. The OD value (Optical Density) was used to calculated RGR% (Relative Generation Rate) of cells, which determined the grade of cytotoxicity. The RGR of nanoparticles of iron oxide and its hydrate are about 1 to 2, which indicate they have just low toxicity.

Magnetic separation of amino acids by gold/iron-oxide composite nanoparticles synthesized by gamma-ray irradiation

2005 ◽  
Vol 293 (1) ◽  
pp. 106-110 ◽  
Author(s):  
Takuya Kinoshita ◽  
Satoshi Seino ◽  
Yoshiteru Mizukoshi ◽  
Yohei Otome ◽  
Takashi Nakagawa ◽  
...  
Keyword(s):  
Amino Acids ◽  
Iron Oxide ◽  
Magnetic Separation ◽  
Gamma Ray ◽  
Composite Nanoparticles ◽  
Oxide Composite ◽  
Gamma Ray Irradiation

scholarly journals The Effect of Hispidulin, a Flavonoid from Salvia plebeia, on Human Nasopharyngeal Carcinoma CNE-2Z Cell Proliferation, Migration, Invasion, and Apoptosis

Molecules ◽  
2021 ◽  
Vol 26 (6) ◽  
pp. 1604
Author(s):  
Yiqun Dai ◽  
Xiaolong Sun ◽  
Bohan Li ◽  
Hui Ma ◽  
Pingping Wu ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Nasopharyngeal Carcinoma ◽  
Migration And Invasion ◽  
Dependent Manner ◽  
Etoh Extract ◽  
Tumor Drug Resistance ◽  
Diphenyltetrazolium Bromide ◽  
Scratch Wound ◽  
Low Toxicity ◽  
Induced Apoptosis

Nasopharyngeal carcinoma (NPC) is a common malignant head and neck tumor. Drug resistance and distant metastasis are the predominant cause of treatment failure in NPC patients. Hispidulin is a flavonoid extracted from the bioassay-guided separation of the EtOH extract of Salvia plebeia with strong anti-proliferative activity in nasopharyngeal carcinoma cells (CNE-2Z). In this study, the effects of hispidulin on proliferation, invasion, migration, and apoptosis were investigated in CNE-2Z cells. The [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] (MTT) assay and the colony formation assay revealed that hispidulin could inhibit CNE-2Z cell proliferation. Hispidulin (25, 50, 100 μM) also induced apoptosis in a dose-dependent manner in CNE-2Z cells. The expression of Akt was reduced, and the expression of the ratio of Bax/Bcl-2 was increased. In addition, scratch wound and transwell assays proved that hispidulin (6.25, 12.5, 25 μM) could inhibited the migration and invasion in CNE-2Z cells. The expressions of HIF-1α, MMP-9, and MMP-2 were decreased, while the MMPs inhibitor TIMP1 was enhanced by hispidulin. Moreover, hispidulin exhibited potent suppression tumor growth and low toxicity in CNE-2Z cancer-bearing mice at a dosage of 20 mg/kg/day. Thus, hispidulin appears to be a potentially effective agent for NPC treatment.

scholarly journals Precipitation of Some Amino Acids in a Crystalline Form in Process of Miso Brewing

1959 ◽  
Vol 33 (13) ◽  
pp. 1130-1133
Author(s):  
Shunro KAWAKISHI ◽  
Susumu HIRANO ◽  
Hisao YOSHII
Keyword(s):  
Amino Acids ◽  
Crystalline Form

scholarly journals Biological Effect of Some Metal Oxides Nanocomposites on Saccharomyces Cerevisiae

2016 ◽  
pp. 41-45
Author(s):  
M. Taran ◽  
K. Shavanova ◽  
N. Starodub
Keyword(s):  
Experimental Data ◽  
Saccharomyces Cerevisiae ◽  
Biological Effect ◽  
Reductase Activity ◽  
Inhibitory Effect ◽  
Yeast Cells ◽  
Yeast Saccharomyces Cerevisiae ◽  
Diphenyltetrazolium Bromide ◽  
Number Of Cells ◽  
Nanostructured Metal

The effect of three types of nanostructured metal-containing saponite clays on brewer’s yeast, Saccharomyces cerevisiae, was investigated. The experimental data show that the studied materials, even though they possess nanometre-sized particles, do not have any inhibitory effect on organisms. In these study a significant increase in the number of cells for S. cerevisiae was demostrated. The largest concentration of cells has been recorded under the influence of a niobium-containing clay, prepared from niobium pentaethoxide at a concentration of 2 mg ml-1. Under these conditions the number of cells was increased up to 5 times in comparison to the control. According to the MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) test, the best results were obtained for both the proton-exchanged and niobium-containing saponite solids at a concentration of 0,5 mg ml-1. They are relatively controlled stimulate mitochondrial reductase activity of yeast cells. Исследовано влияние трех типов наноструктурированных металлосодержащих сапонитовых глин на жизнеспособность пивных дрожжей Saccharomyces cerevisiae. Экспериментальные данные показывают, что исследуемые материалы, несмотря на то, что они нанометрового размера, не проявляют ингибирующее действие на организмы. В исследовании продемонстрировано значительное увеличение количества клеток для S. cerevisiae за действия наноматериалов. Наибольшая концентрация клеток была зарегистрирована при действии нанокомпозита Nb-Sap-EtO в концентрации 2 мг/мл, в этих условиях, количество клеток было увеличено до 5 раз по сравнению с контролем. По данным МТТ теста наблюдается стимулирование активности митохондриальных редуктаз в пивных дрожжах при влиянии наноматериалов, которые содержат в своем составе ниобий в концентрациях от 0,5 мг/мл.

Structural and Biological Overview of Boron-containing Amino Acids in the Medicinal Chemistry Field

2019 ◽  
Vol 26 (26) ◽  
pp. 5077-5089 ◽  
Author(s):  
Antonio Abad García ◽  
Alexey Rayevsky ◽  
E. Andrade-Jorge ◽  
José G. Trujillo-Ferrara
Keyword(s):  
Amino Acids ◽  
Biological Activity ◽  
Biological Effect ◽  
Drug Targets ◽  
New Drugs ◽  
Toxicological Profile ◽  
Therapeutic Tools ◽  
The Stability ◽  
Relationship Of ◽  
Derivatives Of

Background: Amino acids are the basic structural units of proteins as well as the precursors of many compounds with biological activity. The addition of boron reportedly induces changes in the chemical-biological profile of amino acids. Methods: We compiled information on the biological effect of some compounds and discussed the structure-activity relationship of the addition of boron. The specific focus presently is on borinic derivatives of α-amino acids, the specific changes in biological activity caused by the addition of a boron-containing moiety, and the identification of some attractive compounds for testing as potential new drugs. Results: Borinic derivatives of α-amino acids have been widely synthesized and tested as potential new therapeutic tools. The B-N (1.65 A°) or B-C (1.61 A°) or B-O (1.50 A°) bond is often key for the stability at different pHs and temperatures and activity of these compounds. The chemical features of synthesized derivatives, such as the specific moieties and the logP, polarizability and position of the boron atom are clearly linked to their pharmacodynamic and pharmacokinetic profiles. Some mechanisms of action have been suggested or demonstrated, while those responsible for other effects remain unknown. Conclusion: The increasing number of synthetic borinic derivatives of α-amino acids as well as the recently reported crystal structures are providing new insights into the stability of these compounds at different pHs and temperatures, their interactions on drug targets, and the ring formation of five-membered heterocycles. Further research is required to clarify the ways to achieve specific synthesis, the mechanisms involved in the observed biological effect, and the toxicological profile of this type of boron-containing compounds (BCCs).

Free amino acids in the pre-retinal vitreous space. Effect of high potassium and nipecotic acid

1987 ◽  
Vol 44 (2) ◽  
pp. 235-244 ◽  
Author(s):  
Gun Gunnarson ◽  
Ann-Katrin Jakobsson ◽  
Anders Hamberger ◽  
Johan Sjöstrand
Keyword(s):  
Amino Acids ◽  
Free Amino Acids ◽  
Free Amino ◽  
High Potassium ◽  
Nipecotic Acid ◽  
Space Effect

scholarly journals EFFECTS OF ODONTOCEM AND MINERAL TRIOXIDE AGGREGATE-ANGELUS TOXICITY ON FIBROBLAST VIABILITY

2019 ◽  
pp. 32-34
Author(s):  
DEWI SRI KESUMAWATI ◽  
ANGGRAINI MARGONO ◽  
ENDANG SUPRASTIWI
Keyword(s):  
Mineral Trioxide Aggregate ◽  
Fibroblast Cell ◽  
Fibroblast Cells ◽  
Pulp Capping ◽  
Diphenyltetrazolium Bromide ◽  
Significant Difference ◽  
Low Toxicity ◽  
Dental Applications ◽  
Chicken Eggs ◽  
Embryonated Chicken

Objective: Mineral trioxide aggregate (MTA) is proven to be biocompatible and is used for pulp capping treatment. Odontocem, calcium silicate-basedcement similar to MTA with the addition of a steroid, has recently been developed and to compare the effects of Odontocem and MTA-Angelus toxicityon fibroblast cell viability.Methods: Fibroblast cells from embryonated chicken eggs were immersed in Odontocem or MTA-Angelus solution for 24 and 72 h. Cell viability wasanalyzed by Microculture Tetrazolium (MTT) 3-(4,5-dimethylthiazol-2- yl)2,5-diphenyltetrazolium bromide assay.Results: After 24 h, the Odontocem and MTA-Angelus groups showed significantly lower viability (p<0.05) compared with controls. After 72 h, cellviability was significantly higher in the Odontocem and MTA-Angelus groups. However, there was no significant difference between the Odontocemand MTA-Angelus groups.Conclusions: Odontocem and MTA-Angelus have low toxicity for dental applications.

scholarly journals Spectroscopic and Microscopic Analyses of Fe3O4/Au Nanoparticles Obtained by Laser Ablation in Water

Nanomaterials ◽  
2020 ◽  
Vol 10 (1) ◽  
pp. 132 ◽  
Author(s):  
Maurizio Muniz-Miranda ◽  
Francesco Muniz-Miranda ◽  
Emilia Giorgetti
Keyword(s):  
Gold Nanoparticles ◽  
Iron Oxide ◽  
Laser Ablation ◽  
Active Sites ◽  
Aqueous Environment ◽  
Surface Enhanced ◽  
Surface Enhanced Raman ◽  
Enhanced Raman Scattering ◽  
Low Toxicity ◽  
Positively Charged

Magneto-plasmonic nanoparticles constituted of gold and iron oxide were obtained in an aqueous environment by laser ablation of iron and gold targets in two successive steps. Gold nanoparticles are embedded in a mucilaginous matrix of iron oxide, which was identified as magnetite by both microscopic and spectroscopic analyses. The plasmonic properties of the obtained colloids, as well as their adsorption capability, were tested by surface-enhanced Raman scattering (SERS) spectroscopy using 2,2′-bipyridine as a probe molecule. DFT calculations allowed for obtaining information on the adsorption of the ligand molecules that strongly interact with positively charged surface active sites of the gold nanoparticles, thus providing efficient SERS enhancement. The presence of iron oxide gives the bimetallic colloid new possibilities of adsorption in addition to those inherent to gold nanoparticles, especially regarding organic pollutants and heavy metals, allowing to remove them from the aqueous environment by applying a magnetic field. Moreover, these nanoparticles, thanks to their low toxicity, are potentially useful not only in the field of sensors, but also for biomedical applications.

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