In Situ Characterization of Superficial Organic Composition Changes of Thermoacidophilic Archaea Acidianus manzaensis YN-25 in Response to Energy Substrate

2017 ◽  
Vol 262 ◽  
pp. 417-420
Author(s):  
Li Zhu Liu ◽  
Xuan Pan ◽  
Xu Xia ◽  
Yu Hang Zhou ◽  
Zhen Yuan Nie ◽  
...  
Keyword(s):  
Extracellular Polymeric Substances ◽  
Iron Oxidation ◽  
Energy Substrates ◽  
Edge Structure ◽  
Organic Composition ◽  
Thermoacidophilic Archaea ◽  
Acidianus Manzaensis ◽  
First Time

The differences of superficial (about 10 nm) extracellular polymeric substances (EPS) composition of thermoacidophilic archaea Acidianus manzaensis YN-25 acclimated with different energy substrates (FeS2, CuFeS2, S0, FeSO4) were analyzed in situ for the first time by synchrotron radiation (SR) based carbon K-edge X-ray absorption near edge structure (XANES) spectroscopy. The results showed that there are clear associations between the energy substrates and the changes in organic composition in terms of typical function groups (-C=O, -C-O and -C-N). The archaea acclimated with chalcopyrite and pyrite contain higher proportion of proteins but less polysaccharides compared to cells acclimated with S0.The archaea acclimated with FeSO4 contains the highest proportion of protein which is positively associated with the iron oxidation process, while archaea acclimated with S0 contains more lipids and polysaccharides, which may be favorable to the hydrophobic S0 adsorption and utilization processes.

Evolution of Compositions and Contents of Capsule and Slime EPSs for Adaptation to and Action on Energy Substrates and Heavy Metals by Typical Bioleaching Microorganisms

2017 ◽  
Vol 262 ◽  
pp. 466-470 ◽  
Author(s):  
Zhen Yuan Nie ◽  
Hong Chang Liu ◽  
Jin Lan Xia ◽  
Huan Liu ◽  
Yun Lu Cui ◽  
...  
Keyword(s):  
Heavy Metals ◽  
Heavy Metal Ions ◽  
Elemental Sulfur ◽  
Uronic Acids ◽  
Energy Substrates ◽  
Inorganic Matter ◽  
Acidianus Manzaensis ◽  
First Time ◽  
Extracellular Polymer ◽  
Mineral Interaction

Adaption to the energy substrates and heavy metals by bioleaching micoorganisms is the prerequisite for efficient microbe-mineral interaction in bioleaching process. It is known extracellular polymer substances (EPSs) take important role in mediating the adaption to and action on energy substrates and heavy metals. This report presents the evolution of compositions and contents of the major components of EPSs of the typical bioleaching microorganisms (Acidithiobacillus ferrooxidans, Leptospirillum ferriphilum, Sulfobacillus thermosulfidooxidans, and Acidianus manzaensis,) exposed to different energy substrates and heavy metal ions. These strains were acclimated firstly to Fe2+ substrate, and then on the substrates elemental sulfur (S0), pyrite and chalcopyrite, respectively. It was found that the major components of capsule and slime EPSs in terms of proteins, polysaccharides, as well as uronic acids were quite different in contents for the Fe2+-acclimated strains, and they even changed more when the Fe2+-acclimated strains were further acclimated to the other substrates. When exposed to heavy metals, all strains demonstrated much decrease in contents of capsule EPSs, and much increase in slime EPSs contents and the heavy metals were found to bound to the slime parts. It was for the first time the EPSs of the bioleaching strains were fractionated into capsule part and slime part, and it was also for the first time we found the differences in evolution of compositions and contents of the major organic components as well as the inorganic matter of capsule EPSs and slime EPSs when the bioleaching strains were exposed to different energy substrates and heavy metals.

scholarly journals A Previously Uncharacterized, Nonphotosynthetic Member of the Chromatiaceae Is the Primary CO2-Fixing Constituent in a Self-Regenerating Biocathode

2014 ◽  
Vol 81 (2) ◽  
pp. 699-712 ◽  
Author(s):  
Zheng Wang ◽  
Dagmar H. Leary ◽  
Anthony P. Malanoski ◽  
Robert W. Li ◽  
W. Judson Hervey ◽  
...  
Keyword(s):  
Microbial Fuel Cells ◽  
Iron Oxidation ◽  
Extracellular Electron Transfer ◽  
Hydrogen Electrode ◽  
Content Type ◽  
Iron Oxidizing Bacteria ◽  
Distinct Cluster ◽  
The Family

ABSTRACTBiocathode extracellular electron transfer (EET) may be exploited for biotechnology applications, including microbially mediated O2reduction in microbial fuel cells and microbial electrosynthesis. However, biocathode mechanistic studies needed to improve or engineer functionality have been limited to a few select species that form sparse, homogeneous biofilms characterized by little or no growth. Attempts to cultivate isolates from biocathode environmental enrichments often fail due to a lack of some advantage provided by life in a consortium, highlighting the need to study and understand biocathode consortiain situ. Here, we present metagenomic and metaproteomic characterization of a previously described biocathode biofilm (+310 mV versus a standard hydrogen electrode [SHE]) enriched from seawater, reducing O2, and presumably fixing CO2for biomass generation. Metagenomics identified 16 distinct cluster genomes, 15 of which could be assigned at the family or genus level and whose abundance was roughly divided betweenAlpha- andGammaproteobacteria. A total of 644 proteins were identified from shotgun metaproteomics and have been deposited in the the ProteomeXchange with identifier PXD001045. Cluster genomes were used to assign the taxonomic identities of 599 proteins, withMarinobacter,Chromatiaceae, andLabrenziathe most represented. RubisCO and phosphoribulokinase, along with 9 other Calvin-Benson-Bassham cycle proteins, were identified fromChromatiaceae. In addition, proteins similar to those predicted for iron oxidation pathways of known iron-oxidizing bacteria were observed forChromatiaceae. These findings represent the first description of putative EET and CO2fixation mechanisms for a self-regenerating, self-sustaining multispecies biocathode, providing potential targets for functional engineering, as well as new insights into biocathode EET pathways using proteomics.

In Situ Characterization of Relevance of Surface Microstructure and Electrochemical Properties of Chalcopyrite to Adsorption of Acidianus manzaensis

2015 ◽  
Vol 1130 ◽  
pp. 183-187 ◽  
Author(s):  
Jin Lan Xia ◽  
Hong Rui Zhu ◽  
Lei Wang ◽  
Hong Chang Liu ◽  
Zhen Yuan Nie ◽  
...  
Keyword(s):  
Electrochemical Properties ◽  
Electrochemical Treatment ◽  
Specific Adsorption ◽  
Initial Oxidation ◽  
Edge Structure ◽  
X Ray ◽  
Oxidation Potentials ◽  
Before And After ◽  
Acidianus Manzaensis

The in situ relevance of micro- structure and electrochemical properties of chalcopyrite to adsorption of thermoacidophilic bioleaching Archaea Acidianus manzaensis was studied. In this study, the electrochemical behavior of chalcopyrite was first investigated by cyclic voltammetry (CV) to get suitable initial reduction and oxidation potentials, at which electrochemical corrosions of chalcopyrite for several time were performed, respectively, to get specific surface micro-structures. The specific adsorption of A. manzaensis on the electrochemically corroded chalcopyrite surface was then comparatively studied. The changes of microstructure and chemical composition/speciation on the surface of chalcopyrite before and after electrochemical treatment and bio-adsorption was characterized by scanning electron microscopy/electron dispersive spectroscopy (SEM/EDS), and synchrotron radiation-based X-ray diffraction (SR-XRD) and Fe, Cu K-edge X-ray absorption near edge structure (XANES) spectroscopy. The results showed that the suitable initial oxidation and reduction of chalcopyrite electrode were at 0.67 V for 1h and -0.54 V for 10 min, respectively. After treated at 0.67V the surface of chalcopyrite became Cu-deficient with a composition of CuFe1.02S2.15, and bornite (Cu5FeS4) was detected. While after treated at -0.54V, the surface became Fe/S-deficient, with a composition of CuFe0.33S0.81, and a mass of chalcocite and some covellite were detected. Comparing to the original chalcopyrite, the adsorption capacity of A. manzaensis was increased on the surface of oxidation-treatment at 0.67 V, and decreased on the surface of reduction-treatment at -0.54 V. It clearly demonstrates the bornite-containing copper deficient chalcopyrite surface was more preferably adsorbed, whereas the chalcocite-containing Fe/S deficient chalcopyrite surface was less adsorbed by A. manzaensis, indicating the dependence of the specific adsorption of A. manzaensis upon the secondary minerals as well as Fe/S availability in the microstructure of chalcopyrite.

scholarly journals Field characterization of the PM<sub>2.5</sub> Aerosol Chemical Speciation Monitor: insights into the composition, sources, and processes of fine particles in eastern China

2017 ◽  
Vol 17 (23) ◽  
pp. 14501-14517 ◽  
Author(s):  
Yunjiang Zhang ◽  
Lili Tang ◽  
Philip L. Croteau ◽  
Olivier Favez ◽  
Yele Sun ◽  
...  
Keyword(s):  
Chemical Speciation ◽  
Fine Particles ◽  
Eastern China ◽  
Temporal Variations ◽  
Inorganic Aerosols ◽  
Mass Fractions ◽  
Highly Correlated ◽  
First Time

Abstract. A PM2.5-capable aerosol chemical speciation monitor (Q-ACSM) was deployed in urban Nanjing, China, for the first time to measure in situ non-refractory fine particle (NR-PM2.5) composition from 20 October to 19 November 2015, along with parallel measurements of submicron aerosol (PM1) species by a standard Q-ACSM. Our results show that the NR-PM2.5 species (organics, sulfate, nitrate, and ammonium) measured by the PM2.5-Q-ACSM are highly correlated (r2 > 0.9) with those measured by a Sunset Lab OC  /  EC analyzer and a Monitor for AeRosols and GAses (MARGA). The comparisons between the two Q-ACSMs illustrated similar temporal variations in all NR species between PM1 and PM2.5, yet substantial mass fractions of aerosol species were observed in the size range of 1–2.5 µm. On average, NR-PM1−2.5 contributed 53 % of the total NR-PM2.5, with sulfate and secondary organic aerosols (SOAs) being the two largest contributors (26 and 27 %, respectively). Positive matrix factorization of organic aerosol showed similar temporal variations in both primary and secondary OAs between PM1 and PM2.5, although the mass spectra were slightly different due to more thermal decomposition on the capture vaporizer of the PM2.5-Q-ACSM. We observed an enhancement of SOA under high relative humidity conditions, which is associated with simultaneous increases in aerosol pH, gas-phase species (NO2, SO2, and NH3) concentrations and aerosol water content driven by secondary inorganic aerosols. These results likely indicate an enhanced reactive uptake of SOA precursors upon aqueous particles. Therefore, reducing anthropogenic NOx, SO2, and NH3 emissions might not only reduce secondary inorganic aerosols but also the SOA burden during haze episodes in China.

scholarly journals Identification and Characterization of a Rhodopsin Kinase Gene in the Suckers of Octopus vulgaris: Looking around Using Arms?

Biology ◽  
2021 ◽  
Vol 10 (9) ◽  
pp. 936
Author(s):  
Al-Sayed Al-Soudy ◽  
Valeria Maselli ◽  
Stefania Galdiero ◽  
Michael J. Kuba ◽  
Gianluca Polese ◽  
...  
Keyword(s):  
3D Structure ◽  
Complete Characterization ◽  
Octopus Vulgaris ◽  
Kinase Gene ◽  
Light Sensing ◽  
Rhodopsin Kinase ◽  
First Time ◽  
Identification And Characterization

In their foraging behavior octopuses rely on arm search movements outside the visual field of the eyes. In these movements the environment is explored primarily by the suckers that line the entire length of the octopus arm. In this study, for the first time, we report the complete characterization of a light-sensing molecule, Ov-GRK1, in the suckers, skin and retina of Octopus vulgaris. We sequenced the O. vulgaris GRK1 gene, defining a phylogenetic tree and performing a 3D structure model prediction. Furthermore, we found differences in relative mRNA expression in different sucker types at several arm levels, and localized it through in situ hybridization. Our findings suggest that the suckers in octopus arms are much more multimodal than was previously shown, adding the potential for light sensing to the already known mechanical and chemical sensing abilities.

Molecular beam homoepitaxial growth of MgO(001)

1994 ◽  
Vol 9 (11) ◽  
pp. 2944-2952 ◽  
Author(s):  
S.A. Chambers ◽  
T.T. Tran ◽  
T.A. Hileman
Keyword(s):  
Electron Spectroscopy ◽  
High Speed ◽  
Molecular Beam ◽  
Step Flow ◽  
Homoepitaxial Growth ◽  
Crystallographic Order ◽  
Step Flow Growth ◽  
First Time

We describe homoepitaxial growth and detailed in situ characterization of MgO(001). We have used, for the first time, high-speed Auger electron spectroscopy as a real-time probe of film composition during growth. Excellent short-range and long-range crystallographic order are achieved in films grown to a thickness of several hundred angstroms in the substrate temperature range of 450 °C to 750 °C. Moreover, the films become more laminar as the growth temperature increases, suggesting that MgO grows homoepitaxially by the step-flow growth mechanism at elevated temperature. The surfaces of films grown at 650°and 750 °C are smoother than those obtained by cleaving MgO(001).

Elucidating the structure of a high-spin σ-phenyliron(iii) species in a live FeCl3–PhZnCl reaction system

2018 ◽  
Vol 54 (12) ◽  
pp. 1481-1484 ◽  
Author(s):  
Zhiliang Huang ◽  
Dongchao Zhang ◽  
Jyh-Fu Lee ◽  
Aiwen Lei
Keyword(s):  
Density Functional ◽  
Reaction System ◽  
Paramagnetic Resonance ◽  
Functional Theory ◽  
System P ◽  
First Time ◽  
X Ray Absorption ◽  
Electron Paramagnetic

Characterization of σ-aryliron(iii) species in a live reaction system: an unknown sextet Ph(THF)FeCl2 species was well-characterized in a live FeCl3–PhZnCl reaction system for the first time by Raman, in situ IR, electron paramagnetic resonance (EPR), X-ray absorption spectroscopic (XAS) and density functional theory (DFT) calculations.

scholarly journals Fiber-integrated Brillouin microspectroscopy: Towards Brillouin endoscopy

2017 ◽  
Vol 10 (06) ◽  
pp. 1742002 ◽  
Author(s):  
Irina V. Kabakova ◽  
YuChen Xiang ◽  
Carl Paterson ◽  
Peter Török
Keyword(s):  
Optical Fiber ◽  
Brillouin Scattering ◽  
Region Of Interest ◽  
The Body ◽  
Propagation Model ◽  
Micromechanical Characterization ◽  
First Time

Brillouin imaging (BI) for micromechanical characterization of tissues and biomaterials is a fast-developing field of research with a strong potential for medical diagnosis of disease-modified tissues and cells. Although the principles of BI imply its compatibility with in vivo and in situ measurements, the integration of BI with a flexible catheter, capable of reaching the region of interest within the body, is yet to be reported. Here, for the first time, we experimentally investigate integration of the Brillouin spectroscope with standard optical fiber components to achieve a Brillouin endoscope. The performance of single-fiber and dual-fiber endoscopes are demonstrated and analyzed. We show that a major challenge in construction of Brillouin endoscopes is the strong backward Brillouin scattering in the optical fiber and we present a dual-fiber geometry as a possible solution. Measurements of Brillouin spectra in test liquids (water, ethanol and glycerol) are demonstrated using the dual-fiber endoscope and its performance is analyzed numerically with the help of a beam propagation model.

scholarly journals Removal of eDNA from fabrics using a novel laundry DNase revealed using high-resolution imaging

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Hamish C. L. Yau ◽  
Adam K. Malekpour ◽  
Nazarmohammad G. Momin ◽  
Ana L. Morales-García ◽  
William G. T. Willats ◽  
...  
Keyword(s):  
High Resolution ◽  
Extracellular Polymeric Substances ◽  
Dnase I ◽  
Extracellular Dna ◽  
Molecular Probe ◽  
Effective Removal ◽  
Resolution Imaging ◽  
Cleaning Technology ◽  
First Time

AbstractWashed textiles can remain malodorous and dingy due to the recalcitrance of soils. Recent work has found that ‘invisible’ soils such as microbial extracellular DNA (eDNA) play a key role in the adhesion of extracellular polymeric substances that form matrixes contributing to these undesirable characteristics. Here we report the application of an immunostaining method to illustrate the cleaning mechanism of a nuclease (DNase I) acting upon eDNA. Extending previous work that established a key role for eDNA in anchoring these soil matrixes, this work provides new insights into the presence and effective removal of eDNA deposited on fabrics using high-resolution in-situ imaging. Using a monoclonal antibody specific to Z-DNA, we showed that when fabrics are washed with DNase I, the incidence of microbial eDNA is reduced. As well as a quantitative reduction in microbial eDNA, the deep cleaning benefits of this enzyme are shown using confocal microscopy and imaging analysis of T-shirt fibers. To the best of our knowledge, this is the first time the use of a molecular probe has been leveraged for fabric and homecare-related R&D to visualize eDNA and evaluate its removal from textiles by a new-to-laundry DNase enzyme. The approaches described in the current work also have scope for re-application to identify further cleaning technology.

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