EPS Characterization of a Cell Wall-Lacking Archaeon Ferroplasma acidiphilum

2017 ◽  
Vol 262 ◽  
pp. 434-438
Author(s):  
Rui Yong Zhang ◽  
Veronique Blanchard ◽  
Mario Vera Véliz ◽  
Wolfgang Sand
Keyword(s):  
Cell Wall ◽  
Surface Characterization ◽  
Extracellular Polymeric Substances ◽  
Lectin Binding ◽  
Attenuated Total Reflection ◽  
Metal Sulfides ◽  
Mineral Surfaces ◽  
A Cell ◽  
Insight Into

We studied the surface properties of F. acidiphilum DSM 28986 by attenuated total reflection-Fourier transformed infra-red (ATR-FTIR) spectroscopy and microbial adhesion to hydrocarbon (MATH) techniques. In addition, extracellular polymeric substances (EPS) were extracted and characterized by conventional colorimetric analysis and fluorescence lectin-binding analysis (FLBA). Results showed that: 1) cells selectively adhered to mineral surfaces and showed maximum attachment to pyrite of approx. 50% within 30 min; 2) EPS synthesis by F. acidiphilum DSM 28986 was influenced by growth substrates; and 3) tightly-bound EPS (capsular EPS) were composed of carbohydrates and proteins. In contrast, loosely-bound EPS (colloidal EPS) were mainly characterized as carbohydrates. Monosaccharides like glucose, fucose, arabinose, galactose, mannose, and sialic acid were detected in the EPS of F. acidiphilum DSM 28986. This study provides first insight into surface characterization of the cell wall-lacking archaeon F. acidiphilum and facilitates the understanding of interactions of this organism with other acidophiles and metal sulfides.

Isolation and first characterization of a cell-wall-degrading agent of Chlorella

1979 ◽  
Vol 66 (10) ◽  
pp. 525-526 ◽  
Author(s):  
G. Touet ◽  
H. G. Aach
Keyword(s):  
Cell Wall ◽  
A Cell

Tribocorrosion behavior of electroless Ni-P/Ni-B duplex coating on AA7075 aluminum alloy

2019 ◽  
Vol 71 (5) ◽  
pp. 630-635 ◽  
Author(s):  
Harun Mindivan
Keyword(s):  
Aluminum Alloy ◽  
Surface Characterization ◽  
X Ray Diffraction ◽  
Electron Microscopic ◽  
Content Type ◽  
Duplex Coating ◽  
Scanning Electron Microscopic ◽  
Electroless Ni ◽  
Insight Into

Purpose This paper aims to investigate the structural, corrosion and the study of tribocorrosion features of the AA7075 aluminum alloy with and without the application of electroless Ni-P/Ni-B duplex coating with a thickness of approximately 40 microns. Design/methodology/approach Surface characterization of the samples was made by structural surveys (light optic microscope, scanning electron microscopic examinations and X-ray diffraction analyses), hardness measurements, corrosion and tribocorrosion tests. Findings Results of the experiments showed that upper Ni-B coating deposited on the surface of first Ni-P layer by duplex treatment caused remarkable increment in the hardness, corrosion resistance and tribocorrosion performance as compared to the AA7075 aluminum alloy. Originality/value This study can be a practical reference and offers insight into the effects of duplex treating on the increase of hardness, corrosion and tribocorrosion performance.

scholarly journals First Structural Characterization of a Bon-Domain in a Protein from Mycobacterium Tuberculosis: OmpATb Tracks toward an Oligomerization Process to form a Cell Wall Pore

2010 ◽  
Vol 98 (3) ◽  
pp. 648a
Author(s):  
Daniel Auguin ◽  
Yinshan Yang ◽  
Stephane Delbecq ◽  
Emilie Dumas ◽  
Virginie Molle ◽  
...  
Keyword(s):  
Cell Wall ◽  
Mycobacterium Tuberculosis ◽  
Structural Characterization ◽  
A Cell

scholarly journals Fungal α-arabinofuranosidases of glycosyl hydrolase families 51 and 54 show a dual arabinofuranosyl- and galactofuranosyl-hydrolyzing activity

2012 ◽  
Vol 393 (8) ◽  
pp. 767-775 ◽  
Author(s):  
Boris Tefsen ◽  
Ellen L. Lagendijk ◽  
Joohae Park ◽  
Michiel Akeroyd ◽  
Doreen Schachtschabel ◽  
...  
Keyword(s):  
Cell Wall ◽  
Enzyme Activity ◽  
Molecular Modeling ◽  
Aspergillus Niger ◽  
Glycosyl Hydrolase ◽  
Hydrolase Activity ◽  
Gene Encoding ◽  
A Cell ◽  
Or Gene

Abstract Aspergillus niger possesses a galactofuranosidase activity, however, the corresponding enzyme or gene encoding this enzyme has never been identified. As evidence is mounting that enzymes exist with affinity for both arabinofuranose and galactofuranose, we investigated the possibility that α-l-arabinofuranosidases, encoded by the abfA and abfB genes, are responsible for the galactofuranosidase activity of A. niger. Characterization of the recombinant AbfA and AbfB proteins revealed that both enzymes do not only hydrolyze p-nitrophenyl-α-l-arabinofuranoside (pNp-α-Araf) but are also capable of hydrolyzing p-nitrophenyl-β-d-galactofuranoside (pNp-β-Galf). Molecular modeling of the AbfB protein with pNp-β-Galf confirmed the possibility for AbfB to interact with this substrate, similarly as with pNp-α-Araf. We also show that galactomannan, a cell wall compound of A. niger, containing β-linked terminal and internal galactofuranosyl moieties, can be degraded by an enzyme activity that is present in the supernatant of inulin-grown A. niger. Interestingly, purified AbfA and AbfB did not show this hydrolyzing activity toward A. nigergalactomannan. In summary, our studies demonstrate that AbfA and AbfB, α-l-arabinofuranosidases from different families, both contain a galactofuranose (Galf)-hydrolyzing activity. In addition, our data support the presence of a Galf-hydrolase activity expressed by A. niger that is capable of degrading fungal galactomannan.

Structural characterization of a cell wall polysaccharide from Penicillium vermoesenii: chemotaxonomic application

1999 ◽  
Vol 77 (7) ◽  
pp. 961-968 ◽  
Author(s):  
Oussama Ahrazem ◽  
Begoña Gómez-Miranda ◽  
Alicia Prieto ◽  
Isabel Barasoaín ◽  
Manuel Bernabé ◽  
...  
Keyword(s):  
Cell Wall ◽  
Structural Characterization ◽  
Cell Wall Polysaccharide ◽  
A Cell
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