scholarly journals Modification of nutrient agar medium to culture yet-unculturable bacteria living in unsanitary landfills

2021 ◽  
Vol 50 (4) ◽  
pp. 505
Author(s):  
C. Devika ◽  
I. D. Singhalage ◽  
G. Seneviratne
Keyword(s):  
Agar Medium ◽  
Nutrient Agar ◽  
Nutrient Agar Medium ◽  
Unculturable Bacteria

Comparison of a simple butterfat agar medium with other media used for isolation and enumeration of lipolytic bacteria from dairy products

1987 ◽  
Vol 54 (3) ◽  
pp. 413-420 ◽  
Author(s):  
Arthur W. Shelley ◽  
Hilton C. Deeth ◽  
Ian C. MacRae
Keyword(s):  
Melting Point ◽  
Bacterial Growth ◽  
Dairy Products ◽  
Agar Medium ◽  
Nutrient Agar ◽  
Nutrient Agar Medium

SummaryA nutrient agar medium containing 0·1 % of a low melting point fraction of butterfat was shown to be suitable for detection, enumeration and isolation of lipolytic bacteria from milk. Bacterial growth was not inhibited by the butterfat and lipolytic reactions were clearly visible and easily interpreted. Lipolytic counts on the butterfat agar compared favourably with lipolytic counts obtained with other commonly used media.

Enumeration of Polyhydroxyalkanoate (PHA) Producing Bacteria from Dairy Sewage Samples

2019 ◽  
pp. 1-4
Author(s):  
K. Shivalkar Yadav ◽  
D. B. Puranik ◽  
Mohamed Nadeem Fairoze ◽  
R. Prabha
Keyword(s):  
Agar Medium ◽  
Synthetic Polymers ◽  
Synthetic Polymer ◽  
Serial Dilution ◽  
Nutrient Agar ◽  
Sewage Sample ◽  
Bacterial Strains ◽  
Nutrient Agar Medium ◽  
Contemporary Life ◽  
Soil And Water

Aims: The synthetic polymer plastics have become an integral part of contemporary life. Excess use of plastics and indiscriminate dumping of it in soil and water is polluting the environment. In order to overcome this problem, the production and applications of eco-friendly biodegradable products from microbes are becoming inevitable from the last decade and also are the good alternatives for synthetic polymers. Methods and Results: Polyhydroxyalkanoate producing bacterial strains were confirmed by serial dilution of sewage samples from dairies and pour plating using modified nutrient agar medium with 2% glucose and 0.3% sudan black. Commercial dairy sewage sample from III Dairy showed highest count of PHA producers (3.80 log10cfu/ml) followed by II Dairy (3.68 log10cfu/ml) and I Dairy (3.35 log10cfu/ml). On an average, 70 per cent were PHA producers among TBC of sewage samples. Conclusion: Dairy sewage sample from III Dairy showed highest count of PHA producers (3.80log10cfu/ml) Significance and Impact of the Study: This study provides importance of polyhydroxyalkanoates and their role against synthetic plastic by enumerating the polyhydroxyalkanoate (PHA) producing bacteria from Dairy sewage samples that can be effectively utilized for the synthesis of bioplastics.

scholarly journals Study of Microbiological Quality of Biscuits from Street Side Bakery Shops at Dehradun City, India

2020 ◽  
pp. 319-326
Author(s):  
Irtiqa Syed ◽  
Rajendra Prasad ◽  
Adeeba Naaz
Keyword(s):  
Agar Medium ◽  
Microbiological Quality ◽  
Nutrient Agar ◽  
Morphological Observation ◽  
Plate Count ◽  
Bacterial Isolates ◽  
Gram Positive ◽  
Gram Positive Bacteria ◽  
Nutrient Agar Medium

<p>In the present study, microbiological quality of biscuits from street side bakery shops at Dehardun city, India was conducted. A total 11 samples of bakery biscuits were collected randomly and analysed for their microbiologically quality by standard plate count method. Pure cultures of bacterial isolates were prepared by streaking on nutrient agar medium. Bacterial isolates were further studied for morphological characters, culture characters on nutrient agar medium and biochemical testes in laboratory. All samples studies were found contaminated by a variety of bacteria. The highest bacterial load was 2.2 ×105 cfu/g and lowest was 0.1×103 cfu/g, respectively. Morphological observation, culture characters and results of biochemical tests of bacterial isolates were compared with standard results of known bacteria. The bacterial isolated were identified as Alcaligenes faecalis, Bacillus cereus, Micrococcus luteus, Pseudomonas aeruginosa, Streptococcus lactis and Staphylococcus aureus, respectively. Among these, two isolates i.e., A. faecalis and P. aeruginosa were gram negative and other isolates were gram positive bacteria which suggested the poor hygienic conditions inside the bakery during preparation and storage of biscuits. Gram positive bacteria might be contaminated during storage and by bad handling. These food spoiling bacteria might be responsible for food-borne infection and diseases. Therefore, care should be taken while purchasing such products and should be purchased from certified bakery shop.</p>

scholarly journals Perbedaan Daya AntibakteriEkstrak Kulit Kokoa (Theobroma cacao)dan NaOCl 2,5% terhadapPorphyromonas gingivalis

2019 ◽  
Vol 8 (1) ◽  
pp. 49
Author(s):  
Tamara Yuanita ◽  
Rifatul Jannah ◽  
Edhie Arif Pasetyo ◽  
Setyabudi Setyabudi
Keyword(s):  
Antibacterial Activity ◽  
Porphyromonas Gingivalis ◽  
Root Canal ◽  
Theobroma Cacao ◽  
Agar Medium ◽  
Nutrient Agar ◽  
Negative Effects ◽  
Root Canal Disinfection ◽  
Nutrient Agar Medium ◽  
The Difference

Background: Since pulp infection plays an important role in the development of periradicular lesions, endodontic treatment should be directed to eliminate bacterial and theirproducts. However, currently 20% of cases of apical periodontitis are not resolved after root canal treatment and therefore required for new root canal disinfection. The most commonly used irrigation material today is NaOCl 2.5%. However, NaOCl has negative effects, including being toxic when the material is injected into the periradicular tissue causing extensive pain, bleeding and swelling. Until now, many drugs come from plants that are still produced from plant extracts. One of the plants that can be utilized is cocoa (Theobroma cacao). Cocoa contains active compounds, such as saponins, tannins, alkaloids, flanonoids, aromatic terpenoids, theobromins and other metabolites. Cocoa husk has been studied to have an antibacterial effect on Porphyromonas gingivalis which is the main bacterial cause of apical periodontal. However, the difference in antibacterial activity between cocoa husk extract and NaOCl 2.5%  againstPorphyromonas gingivalis has not been studied. Porpuse:The aim of this study is to compare antibacterial activity of cocoa husk extract and NaOCl 2.5% againstPorphyromonas gingivalis.Method: This research was a laboratory experimental study. Porphyromonas gingivalis were swabbed to nutrient agar medium. Consequently, cocoa husk extract 25% and NaOCl 2.5% were placed in wells of 5mm diameter and nutrient agar medium. The diameter of the zone of inhibition around the test materials was measured after 24 hours.Result:Cocoa husk extract has lower mean inhibitory zone diameter (14.22) than NaOCl 2.5% (16.06). Conclusion:Cocoa husk extract has lower antibacterial activity against Porphyromonas gingivalis compared to NaOCl 2.5%.

Interactions Between Some Tubificid Oligochaetes and Bacteria Found in the Sediments of Toronto Harbour, Ontario

1971 ◽  
Vol 28 (3) ◽  
pp. 335-341 ◽  
Author(s):  
M. Wavre ◽  
R. O. Brinkhurst
Keyword(s):  
Relative Abundance ◽  
Agar Medium ◽  
Bacterial Flora ◽  
Nutrient Agar ◽  
Aerobic Bacteria ◽  
Starvation Period ◽  
Physical Proximity ◽  
Close Physical Proximity ◽  
Nutrient Agar Medium

Three species of tubificid oligochaetes and seven heterotrophic aerobic bacteria were identified from the sediment of Toronto Harbour, Ontario. The relative abundance of the identifiable bacteria present in the mud and in the worm feces voided after 18 hr was established using nutrient agar medium. The results indicated a reduction in the relative abundance of bacteria by about 72% and a reduction in the variety of bacteria in the feces as opposed to those detected in the mud. After a starvation period of approximately 1 week, only one type of bacteria was detected in the feces and guts of the worms, whereas all seven were shown to be ingested at one time or another. The surviving bacterium was different in each tubificid species, but Aeromonas sp. was seen to replace this surviving species on some occasions in all three species of worm. The results suggest that the fraction of the bacterial flora investigated forms a part of the diet of tubificids, and that differences between worm species in relation to their use of those bacteria may help to explain the survival of a number of morphologically unspecialized sediment-feeding species in close physical proximity to each other.

scholarly journals Screening, Isolation and Biochemical Characterization of Laccase Producing Bacteria from Contaminated Sites

2021 ◽  
Vol 71 (2) ◽  
Author(s):  
Siva Jyothi J. ◽  
Kishore Kumar K. ◽  
Hema Latha E.
Keyword(s):  
Biochemical Characterization ◽  
Agar Medium ◽  
Nutrient Agar ◽  
Laccase Production ◽  
Bacterial Strains ◽  
Isolation And Identification ◽  
Leading Role ◽  
Degrading Bacteria ◽  
Nutrient Agar Medium ◽  
Guntur District

Screening and isolation of Laccase producing bacteria from Guntur District soil was carried out to assess the diversity of Lignocellulose degrading bacteria. Isolation and identification of environmental friendly bacteria for lignin degradation becomes an essential one, because all the researchers are mainly concentrating on fungal strains. However, bacteria seem to play a leading role in decomposing lignin. For isolation of Laccase producing bacteria nutrient agar medium containing guaiacol was used. Total nine bacterial strains were isolated from soil samples collected from different regions of Guntur district. Preliminary screening of bacterial strains was carried out on guaiacol containing nutrient agar medium for laccase production. Formation of green colour using ABTS (2,2'- azinobis(3-ethylbenzthiazoline-6-sulfonate) confirms the capability of laccase production by the bacterial strains. Nine bacterial strains showed positive results. High laccase producing bacterial isolates were examined for morphological and biochemical characteristics according to Bergey’s Manual of Systematic Bacteriology. The predominant isolates were identified as Bacillus and Enterobacter species.

scholarly journals FEATURES OF CYST FORMATION IN АZOTOBACTER VINELANDII AND АZOTOBACTER CHROOCOCCUM UNDER THE INFLUENCE OF TEMPERATURE AND HUMIDITY

2012 ◽  
Vol 14 ◽  
pp. 91-99
Author(s):  
V.M. Nesterenko ◽  
S.F. Kozar ◽  
T.A. Zherebor ◽  
T.O. Usmanova
Keyword(s):  
Agar Medium ◽  
Cyst Formation ◽  
Nutrient Agar ◽  
Influence Of Temperature ◽  
Nutrient Agar Medium ◽  
Temperature And Humidity

The paper studies the influence of temperature and humidity on the induction of cyst formation by the bacteria А. vinelandii М-Х, А. chroococcum M-70 and consortium of А. vinelandii and А. chroococcum. It was shown that temperature 44 ºС was the optimal for cyst formation by Аzotobacter in nutrient agar medium. Its drying for 5 days at this temperature (44 ºС) is optimal for encystment in the powdered substrate.

scholarly journals Suppressors of ‘blue’ mutations in yeast

1974 ◽  
Vol 23 (1) ◽  
pp. 37-45 ◽  
Author(s):  
I. de G. Mitchell ◽  
E. A. Bevan
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Methylene Blue ◽  
Temperature Sensitivity ◽  
High Frequency ◽  
Agar Medium ◽  
Nuclear Gene ◽  
Nutrient Agar ◽  
Wild Type ◽  
Cytoplasmic Factor ◽  
Nutrient Agar Medium

SummaryMutants ofSaccharomyces cerevisiaewhose colonies were blue when grown on nutrient agar medium containing methylene blue reverted to wild-type with white-colony phenotype at high frequency. This reversion was controlled by nuclear gene suppressors in some mutants, and by cytoplasmic suppressors in others. Each of the latter suppressed several independetly segregating blue mutants. These suppressors could be divided into two classes: suppression by petite mutations which behaved as recessives, and suppression by a cytoplasmic factor in respiration-sufficient cells which behaved as dominant over wild-type but might also be a mutation ofrho. A relationship between blue mutation and temperature sensitivity was suggested.

Deposit assessment of Bacillus thuringiensis formulations applied from an aircraft

1980 ◽  
Vol 26 (11) ◽  
pp. 1364-1366 ◽  
Author(s):  
W. A. Smirnoff
Keyword(s):  
Bacillus Thuringiensis ◽  
Agar Medium ◽  
Nutrient Agar ◽  
Treatment Efficiency ◽  
New Approach ◽  
Nutrient Agar Medium ◽  
Petri Dishes ◽  
Aerial Spraying

Deposit assessment after aerial spraying with Bacillus thuringiensis (B.t.) formulations consists of the analysis of the splash of droplets on Kromekote papers and determining the number of bacterial colonies growing on nutrient agar medium in Petri dishes. However, neither of these two methods provides a number of spores deposited although this figure is essential to determine treatment efficiency. Both methods are evaluated with regard to this need and a new approach is suggested.

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