scholarly journals B7-1/2 (CD80/CD86) Direct Signaling to B Cells Enhances IgG Secretion

2009 ◽  
Vol 183 (12) ◽  
pp. 7661-7671 ◽  
Author(s):  
Friederike C. Rau ◽  
Jacquelyn Dieter ◽  
Zhang Luo ◽  
Stephen O. Priest ◽  
Nicole Baumgarth
Keyword(s):  
B Cells ◽  
Igg Secretion

scholarly journals Interleukin-10 induces immunoglobulin G isotype switch recombination in human CD40-activated naive B lymphocytes.

1996 ◽  
Vol 183 (3) ◽  
pp. 937-947 ◽  
Author(s):  
F Malisan ◽  
F Brière ◽  
J M Bridon ◽  
N Harindranath ◽  
F C Mills ◽  
...  
Keyword(s):  
B Cells ◽  
B Lymphocytes ◽  
Immunoglobulin G ◽  
Dna Recombination ◽  
Interleukin 10 ◽  
Human Igg ◽  
By Products ◽  
Igg Secretion

Upon activation, B lymphocytes can change the isotype of the antibody they express by immunoglobulin (Ig) isotype switch recombination. In previous studies on the regulation of human IgG expression, we demonstrated that interleukin 10 (IL-10) could stimulate IgG1 and IgG3 secretion by human CD40-activated naive (sIgD+) tonsillar B cells. To assess whether IL-10 actually promotes the DNA recombination underlying switching to these isotypes, we examined the effect of IL-10 on the generation of reciprocal products that form DNA circles as by-products of switch recombination. The content of reciprocal products characteristic of mu-gamma recombination was elevated after culture of CD40-activated tonsillar sIgD+ B cells with either IL-4 or IL-10, although high levels of IgG secretion were observed only with IL-10. Unlike IL-4, IL-10 did not induce reciprocal products of mu-epsilon and gamma-epsilon switch recombination. These results demonstrate that IL-10 promotes both switching to gamma and IgG secretion.

Immunoglobulin G-binding factors (IgG-BF) inhibit IgG secretion by, as well as proliferation of, hybridoma B cells

1987 ◽  
Vol 16 (2) ◽  
pp. 139-144 ◽  
Author(s):  
J.L. Teillaud ◽  
S. Amigorena ◽  
J. Moncuit ◽  
C. Sautès ◽  
W.H. Fridman
Keyword(s):  
B Cells ◽  
Immunoglobulin G ◽  
Igg Secretion

scholarly journals Modulation of Single-Cell IgG Secretion Frequency and Rates in Human Memory B Cells by CpG DNA, CD40L, IL-21, and Cell Division

2009 ◽  
Vol 183 (5) ◽  
pp. 3177-3187 ◽  
Author(s):  
Alicia D. Henn ◽  
Jonathan Rebhahn ◽  
Miguel A. Brown ◽  
Alison J. Murphy ◽  
Mircea N. Coca ◽  
...  
Keyword(s):  
Cell Division ◽  
B Cells ◽  
Single Cell ◽  
Human Memory ◽  
Memory B Cells ◽  
Cpg Dna ◽  
Igg Secretion

scholarly journals The switch from IgM to IgG secretion in single mitogen-stimulated B-cell clones.

1978 ◽  
Vol 147 (6) ◽  
pp. 1744-1754 ◽  
Author(s):  
J Andersson ◽  
A Coutinho ◽  
F Melchers
Keyword(s):  
B Cells ◽  
B Cell ◽  
Clonal Growth ◽  
Cell Clone ◽  
Culture Conditions ◽  
Secreting Cell ◽  
Exact Test ◽  
Cell Clones ◽  
Igg Secretion

The frequency of mitogen-reactive B cells yielding an IgG plaque-forming cell (PFC) response has been determined in vitro by limiting dilution analysis under culture conditions which allow every growth-induced B cell to grow and mature into a clone of Ig-secreting cells. The frequencies of lipopolysaccharide (LPS)-and lipoprotein-reactive precursors for IgG-secreting cells in the spleen of 6--8 wk old C3H/Tif and of C57BL/67 mice were found to be between 1 in 30 and 1 in 40 B cells and, therefore, only one tenth of the frequencies of mitogen-reactive precursors of clones secreting IgM. All IgG-secreting cells developed by switching in clones which previously contained IgM-secreting cells. This was shown in two experiments where the total number of mitogen-reactive precursor yielding IgM-secreting cell clones was limited such that 82 or 90% of all responding cultures originated from one precursor. Thus, of 480 cultures in the first and 720 cultures in the second experiment, 86 and 98 cultures were found positive, yielding IgM-secreting cells at day 5. When the same cultures were assayed at day 7 for IgG-secreting cells 9 and 10 cultures were found positive. All 19 cultures with IgG-secreting cells previously had contained IgM-secreting cells. The probability that IgG-secreting cells and IgM-secreting cells would have arisen from independent precursors can be calculated using Fisher's exact test of independence. For the two experiments those probabilities are 3.4 X 10(-7) and 4.0 X 10(-9). Since we have previously shown that each cell in a mitogen-stimulated, growing B-cell clone divides, and that each dividing cell secretes Ig, we conclude from these experiments that the large majority--in our experiments all--of the IgG-secreting cells in mitogen-stimulated B-cell clones develop by switch from IgM-secreting cells. IgG-secreting cells develop either early or late during growth of a single IgM-secreting cell clone. The switch to IgG secretion, therefore, is not fixed in the time of clonal growth after mitogenic stimulation.

scholarly journals T cell-derived B cell growth and differentiation factors. Dichotomy between the responsiveness of B cells from adult and neonatal mice.

1983 ◽  
Vol 157 (2) ◽  
pp. 600-612 ◽  
Author(s):  
E Pure ◽  
P C Isakson ◽  
J W Kappler ◽  
P Marrack ◽  
P H Krammer ◽  
...  
Keyword(s):  
T Cell ◽  
B Cells ◽  
Cell Growth ◽  
B Cell ◽  
Immunoglobulin M ◽  
B Cell Differentiation ◽  
Neonatal Mice ◽  
Differentiation Factors ◽  
Immature B Cells ◽  
Igg Secretion

In these studies we have determined the molecular weights of B cell growth factor (BCGF) (less than 20,000), and B cell differentiation factors (BCDF) that induce immunoglobulin M (IgM) secretion (BCDF mu) (30-60,000) and IgG secretion (BCDF gamma) (less than 20,000). Thus, the molecular weight of BCDF mu is distinct from that of BCGF and BCDF gamma; BCGF and BCDF gamma cannot be distinguished. In addition, BCGF, BCDF mu, and BCDF gamma are distinguishable by their presence or absence in different supernatants from a panel of mitogen-induced T cell clones. These results suggest that the three lymphokines are different. This conclusion is supported by their differential biological effect on B cells from adult and neonatal mice. Thus, treatment with anti-Ig induces B cells from adult mice to proliferate and this proliferation is sustained by BCGF. In contrast, even in the presence of BCGF, anti-Ig does not induce B cells from neonatal mice to proliferate. However, BCDF mu and BCDF gamma induce IgM and IgG secretion in B cells, respectively, from both adult and neonatal mice. Thus, mature B cells can both clonally expand and differentiate in response to anti-Ig, BCGF, and BCDF, whereas immature B cells can only differentiate. The poor response of neonatal B cells to anti-Ig and BCGF may partially explain the relative immunoincompetence of immature B cells.

scholarly journals Human CD4<sup>-</sup> CD8<sup>-</sup> Invariant Natural Killer T Cells Promote IgG Secretion from B Cells Stimulated by Cross-Linking of Their Antigen Receptors

2016 ◽  
Vol 06 (02) ◽  
pp. 34-41
Author(s):  
Tomomitsu Miyasaka ◽  
Yurie Watanabe ◽  
Yukiko Akahori ◽  
Namiko Miyamura ◽  
Keiko Ishii ◽  
...  
Keyword(s):  
T Cells ◽  
B Cells ◽  
Natural Killer ◽  
Natural Killer T Cells ◽  
Cross Linking ◽  
Antigen Receptors ◽  
Killer T Cells ◽  
Invariant Natural Killer ◽  
Igg Secretion ◽  
Natural Killer T

T Cell-Derived Lymphokines That Induce IgM and IgG Secretion in Activated Murine B Cells

1984 ◽  
Vol 78 (1) ◽  
pp. 137-157 ◽  
Author(s):  
E. S. Vitetta ◽  
K. Brooks ◽  
Y.-W. Chen ◽  
P. Isakson ◽  
S. Jones ◽  
...  
Keyword(s):  
T Cell ◽  
B Cells ◽  
Igg Secretion

scholarly journals B cells capable of spontaneous IgG secretion in cerebrospinal fluid from patients with multiple sclerosis: dependency on local IL-6 production

2008 ◽  
Vol 101 (3) ◽  
pp. 449-452 ◽  
Author(s):  
L. PEREZ ◽  
J. C. ALVAREZ-CERMEÑO ◽  
C. RODRIGUEZ ◽  
E. ROLDÁN ◽  
J. A. BRIEVA
Keyword(s):  
Multiple Sclerosis ◽  
Cerebrospinal Fluid ◽  
B Cells ◽  
Igg Secretion

scholarly journals Splenic immunoglobulin-secreting cells and their regulation in autoimmune mice.

1980 ◽  
Vol 151 (2) ◽  
pp. 446-466 ◽  
Author(s):  
A N Theofilopoulos ◽  
D L Shawler ◽  
R A Eisenberg ◽  
F J Dixon
Keyword(s):  
T Cells ◽  
B Cells ◽  
High Frequency ◽  
Spleen Cells ◽  
Con A ◽  
Clinical Onset ◽  
Normal Spleen ◽  
Igg Secretion ◽  
Bxsb Mice

We have investigated in vitro the magnitude, nature, and regulation of spontaneous and mitogen-induced Ig secretion by splenic lymphocytes from several autoimmune murine strains (NZB, NZB X W, MRL/l BXSB) and appropriate, normal mice. All autoimmune strains had increased numbers of mature splenic B lymphocytes, which secreted and/or contained Ig, compared to age-matched normal strains. In NZB and NZB X W mice, the high frequency of mature B cells was apparent early in life, whereas in MRL/l and BXSB mice it was first noted shortly before the clinical onset of disease. Spleen cells from young autoimmune mice of all four strains secreted predominantly IgM, but with aging and the appearance of disease, the cells switched to IgG secretion predominantly. In contrast, spleen cells from normal mice were predominantly IgM, but with aging and the appearance of disease, the cells switched to IgG secretion predominantly. In contrast, spleen cells from normal mice were predominantly IgM secretors throughout the animals' lives. Approximately 15% of the total Ig-secreting cells in older NZB, NZB X W, and MRL mice were committed to secretion of anti-ssDNA antibodies. In both autoimmune and normal spleen cells, the B-cell population alone contained fewer secreting cells than the total lymphocyte population, indicating that T cells were required to achieve maximal levels of plaque-forming cells. Spleen cells of NZB and NZB X W mice had a greater response to lipopolysaccharide (LPS) than other autoimmune and normal strains. Responsiveness to LPS, as measured by the frequency of induced Ig-secreting cells, was considerably diminished with age and onset of disease in all autoimmune but not in normal strains. LPS-induced Ig secretion by B cells of autoimmune and normal mice was subject to regulation by splenic T cells. No significant differences were observed between concanavalin-A (Con A) stimulated spleen cells from young and older autoimmune mice and normal control strains in effectively suppressing spontaneous and LPS-induced Ig secretion. Moreover, B cells from autoimmune mice and from normal strains were equally receptive to Con A-induced suppressor signals. T cells from young and older NZB and BXSB mice added to a standard number of B cells from syngeneic young mice provided equal help in enhancing LPS-induced Ig secretion, and this help in turn was equivalent to that provided by T cells from normal mice of the same H-2 haplotype. The exception was the MRL/l strain; T cells from older animals provided considerably more help than T cells from young MRL/l or T cells from young and older H-2-compatible normal mice.

Plasmalemma localisation of inorganic phosphate in B cells pancreas

1978 ◽  
Vol 36 (2) ◽  
pp. 528-529
Author(s):  
F. B. P. Wooding ◽  
K. Pedley ◽  
N. Freinkel ◽  
R. M. C. Dawson
Keyword(s):  
Electron Microscope ◽  
B Cells ◽  
B Cell ◽  
Pancreatic Islets ◽  
High Glucose ◽  
Lead Acetate ◽  
Inorganic Phosphate ◽  
Slight Modification ◽  
Uranyl Acetate ◽  
Lead Phosphate

Freinkel et al (1974) demonstrated that isolated perifused rat pancreatic islets reproduceably release up to 50% of their total inorganic phosphate when the concentration of glucose in the perifusion medium is raised.Using a slight modification of the Libanati and Tandler (1969) method for localising inorganic phosphate by fixation-precipitation with glutaraldehyde-lead acetate we can demonstrate there is a significant deposition of lead phosphate (identified by energy dispersive electron microscope microanalysis) at or on the plasmalemma of the B cell of the islets (Fig 1, 3). Islets after incubation in high glucose show very little precipitate at this or any other site (Fig 2). At higher magnification the precipitate seems to be intracellular (Fig 4) but since any use of osmium or uranyl acetate to increase membrane contrast removes the precipitate of lead phosphate it has not been possible to verify this as yet.

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