scholarly journals Lineage−Sca1+c-Kit−CD25+ Cells Are IL-33–Responsive Type 2 Innate Cells in the Mouse Bone Marrow

2011 ◽  
Vol 187 (11) ◽  
pp. 5795-5804 ◽  
Author(s):  
Adipong Brickshawana ◽  
Virginia Smith Shapiro ◽  
Hirohito Kita ◽  
Larry R. Pease
Keyword(s):  
Bone Marrow ◽  
Mouse Bone Marrow

Abstract 623: Hyperhomocysteinemia Potentiates Inflammatory Monocyte Differentiation and Vascular Dysfunction in Type 2 Diabetic Mice

2017 ◽  
Vol 37 (suppl_1) ◽  
Author(s):  
Pu Fang ◽  
Xiaohua Jiang ◽  
Xiaofeng Yang ◽  
Hong Wang
Keyword(s):  
Bone Marrow ◽  
Vascular Function ◽  
Mononuclear Cells ◽  
Vascular Dysfunction ◽  
Spontaneous Mutation ◽  
Mouse Bone Marrow ◽  
Inflammatory Monocyte ◽  
Inflammatory Monocytes ◽  
Endothelial Nitric Oxide

Hyperhomocysteinemia (HHcy) is associated with increased diabetic cardiovascular diseases. In this study, we investigated the effects of type 2 diabetes mellitus (T2DM), HHcy and their combination on inflammatory monocyte (MC) differentiation and vascular function. We established an atherosclerosis-susceptible T2DM+HHcy compound mouse model. T2DM was induced in db/db mice by spontaneous mutation (average blood glucose 493 mg/dL). HHcy was established by feeding the mice a high fat+high methionine diet (HF+HM, 8 weeks). Methionine is the homocysteine (Hcy) precursor, which elevated plasma Hcy level to 129 μM. Plasma Hcy level was lowered by vitamin therapy employing a HF+HM+high vitamin diet (HF+HM+HV), which reduced plasma Hcy levels from 129 μM to 42 μM. HHcy aggravated T2DM-impaired endothelial-dependent vessel relaxation to acetylcholine, which was completely abolished by endothelial nitric oxide synthase (eNOS) inhibitor N G -nitro-L-arginine methyl ester. HHcy potentiated T2DM-induced inflammatory monocytes (CD45 + CD11b + Ly6C + MCs) in aorta isolated from compound mice. Severe HHcy- and T2DM-induced mononuclear cells (MNCs), CD11b + MCs, CD11b + Ly6C middle+high inflammatory MCs and M1 macrophages (MØs) were potentiated by the combination of HHcy and T2DM in mouse bone marrow (BM), peripheral blood, and spleen. Folate based Hcy-lowering therapy (HF+HM+HV) reversed systemic MNC, MC, inflammatory MC and MØ and aortic inflammatory MC increases in T2DM mice. Finally, bone marrow transplantation (BMT) using lentivirus-shLy6C transduced BM cells, which decreased blood Ly6C + inflammatory MCs, resulted in ameliorated endothelial-dependent vessel relaxation. In conclusion, our data suggested that HHcy accelerated T2DM-induced systemic inflammatory MC and MØ differentiation, aortic inflammatory MC infiltration, and vascular dysfunction.

In Vitro, In Silico and Ex Vivo Studies of Dihydroartemisinin Derivatives as Antitubercular Agents

2019 ◽  
Vol 19 (8) ◽  
pp. 633-644 ◽  
Author(s):  
Komal Kalani ◽  
Sarfaraz Alam ◽  
Vinita Chaturvedi ◽  
Shyam Singh ◽  
Feroz Khan ◽  
...  
Keyword(s):  
Bone Marrow ◽  
In Silico ◽  
Ex Vivo ◽  
Virulent Strain ◽  
Infection Model ◽  
Mouse Bone Marrow ◽  
Significant Activity ◽  
Macrophage Infection ◽  
In Silico Studies

Introduction: As a part of our drug discovery program for anti-tubercular agents, dihydroartemisinin (DHA-1) was screened against Mtb H37Rv, which showed moderate anti-tubercular activity (>25.0 µg/mL). These results prompted us to carry out the chemical transformation of DHA-1 into various derivatives and study their antitubercular potential. Materials and Methods: DHA-1 was semi-synthetically converted into four new acyl derivatives (DHA-1A – DHA-1D) and in-vitro evaluated for their anti-tubercular potential against Mycobacterium tuberculosis H37Rv virulent strain. The derivatives, DHA-1C (12-O-(4-nitro) benzoyl; MIC 12.5 µg/mL) and DHA-1D (12-O-chloro acetyl; MIC 3.12µg/mL) showed significant activity against the pathogen. Results: In silico studies of the most active derivative (DHA-1D) showed interaction with ARG448 inhibiting the mycobacterium enzymes. Additionally, it showed no cytotoxicity towards the Vero C1008 cells and Mouse bone marrow derived macrophages. Conclusion: DHA-1D killed 62% intracellular M. tuberculosis in Mouse bone marrow macrophage infection model. To the best of our knowledge, this is the first-ever report on the antitubercular potential of dihydroartemisinin and its derivatives. Since dihydroartemisinin is widely used as an antimalarial drug; these results may be of great help in anti-tubercular drug development from a very common, inexpensive, and non-toxic natural product.

Recent approaches for isolating and culturing mouse bone marrow-derived mesenchymal stromal stem cells

2020 ◽  
Vol 15 ◽  
Author(s):  
Basem M. Abdallah ◽  
Hany M. Khattab
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Molecular Mechanisms ◽  
Replicative Senescence ◽  
Cellular Heterogeneity ◽  
High Yield ◽  
Mouse Strains ◽  
Mouse Bone Marrow ◽  
Differentiation Potential ◽  
Stromal Stem Cells

: The isolation and culture of murine bone marrow-derived mesenchymal stromal stem cells (mBMSCs) have attracted great interest in terms of the pre-clinical applications of stem cells in tissue engineering and regenerative medicine. In addition, culturing mBMSCs is important for studying the molecular mechanisms of bone remodelling using relevant transgenic mice. Several factors have created challenges in the isolation and high-yield expansion of homogenous mBMSCs; these factors include low frequencies of bone marrow-derived mesenchymal stromal stem cells (BMSCs) in bone marrow, variation among inbred mouse strains, contamination with haematopoietic progenitor cells (HPCs), the replicative senescence phenotype and cellular heterogeneity. In this review, we provide an overview of nearly all protocols used for isolating and culturing mBMSCs with the aim of clarifying the most important guidelines for culturing highly purified mBMSC populations retaining in vitro and in vivo differentiation potential.

Isolation of Multipotent Stromal Cells from Mouse Bone Marrow

BIO-PROTOCOL ◽  
2014 ◽  
Vol 4 (4) ◽  
Author(s):  
Aurélie Tormo ◽  
Moutih Rafei ◽  
Jean-François Gauchat
Keyword(s):  
Bone Marrow ◽  
Stromal Cells ◽  
Mouse Bone Marrow ◽  
Multipotent Stromal Cells

Differentiation of Mouse Bone-Marrow Mesenchymal Stem Cells into Motor Neuron Cells in vitro

2016 ◽  
Vol 19 (2) ◽  
pp. 111-116
Author(s):  
Rafal Hussamildeen Abdullah ◽  
◽  
Shahlla Mahdi Salih ◽  
Nahi Yosef Yaseen ◽  
Ahmed Majeed Al-Shammari ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Motor Neuron ◽  
Mouse Bone Marrow ◽  
Marrow Mesenchymal Stem Cells

scholarly journals Experimental Type 2 Diabetes Differently Impacts on the Select Functions of Bone Marrow-Derived Multipotent Stromal Cells

Cells ◽  
2021 ◽  
Vol 10 (2) ◽  
pp. 268
Author(s):  
Jonathan Ribot ◽  
Cyprien Denoeud ◽  
Guilhem Frescaline ◽  
Rebecca Landon ◽  
Hervé Petite ◽  
...  
Keyword(s):  
Type 2 Diabetes ◽  
Bone Marrow ◽  
Stromal Cells ◽  
Adipogenic Differentiation ◽  
Therapeutic Modality ◽  
Multipotent Stromal Cells ◽  
Cell Therapies

Bone marrow-derived multipotent stromal cells (BMMSCs) represent an attractive therapeutic modality for cell therapy in type 2 diabetes mellitus (T2DM)-associated complications. T2DM changes the bone marrow environment; however, its effects on BMMSC properties remain unclear. The present study aimed at investigating select functions and differentiation of BMMSCs harvested from the T2DM microenvironment as potential candidates for regenerative medicine. BMMSCs were obtained from Zucker diabetic fatty (ZDF; an obese-T2DM model) rats and their lean littermates (ZL; controls), and cultured under normoglycemic conditions. The BMMSCs derived from ZDF animals were fewer in number, with limited clonogenicity (by 2-fold), adhesion (by 2.9-fold), proliferation (by 50%), migration capability (by 25%), and increased apoptosis rate (by 2.5-fold) compared to their ZL counterparts. Compared to the cultured ZL-BMMSCs, the ZDF-BMMSCs exhibited (i) enhanced adipogenic differentiation (increased number of lipid droplets by 2-fold; upregulation of the Pparg, AdipoQ, and Fabp genes), possibly due to having been primed to undergo such differentiation in vivo prior to cell isolation, and (ii) different angiogenesis-related gene expression in vitro and decreased proangiogenic potential after transplantation in nude mice. These results provided evidence that the T2DM environment impairs BMMSC expansion and select functions pertinent to their efficacy when used in autologous cell therapies.

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