scholarly journals Label-Free Biosensors for Cell Biology

2011 ◽  
Vol 2011 ◽  
pp. 1-16 ◽  
Author(s):  
Ye Fang
Keyword(s):  
High Throughput ◽  
Molecular Interaction ◽  
Cell Biology ◽  
Historical Development ◽  
Interaction Analysis ◽  
Analytical Tool ◽  
Label Free ◽  
Future Perspectives ◽  
Cell Level ◽  
Recent Developments

Label-free biosensors for studying cell biology have finally come of age. Recent developments have advanced the biosensors from low throughput and high maintenance research tools to high throughput and low maintenance screening platforms. In parallel, the biosensors have evolved from an analytical tool solely for molecular interaction analysis to powerful platforms for studying cell biology at the whole cell level. This paper presents historical development, detection principles, and applications in cell biology of label-free biosensors. Future perspectives are also discussed.

scholarly journals Methods for protein delivery into cells: from current approaches to future perspectives

2020 ◽  
Vol 48 (2) ◽  
pp. 357-365
Author(s):  
Chalmers Chau ◽  
Paolo Actis ◽  
Eric Hewitt
Keyword(s):  
Single Molecule ◽  
Cell Biology ◽  
Protein Delivery ◽  
Mammalian Cells ◽  
Cultured Cells ◽  
Cellular Damage ◽  
Future Perspectives ◽  
Chemically Modified ◽  
Recent Developments ◽  
Direct Delivery

The manipulation of cultured mammalian cells by the delivery of exogenous macromolecules is one of the cornerstones of experimental cell biology. Although the transfection of cells with DNA expressions constructs that encode proteins is routine and simple to perform, the direct delivery of proteins into cells has many advantages. For example, proteins can be chemically modified, assembled into defined complexes and subject to biophysical analyses prior to their delivery into cells. Here, we review new approaches to the injection and electroporation of proteins into cultured cells. In particular, we focus on how recent developments in nanoscale injection probes and localized electroporation devices enable proteins to be delivered whilst minimizing cellular damage. Moreover, we discuss how nanopore sensing may ultimately enable the quantification of protein delivery at single-molecule resolution.

scholarly journals MICRO-SCALE TECHNOLOGIES FOR CELL MECHANICS: EXPERIMENTAL AND MODELLING APPROACHES

2018 ◽  
Author(s):  
Federica Caselli ◽  
Nicola A. Nodargi ◽  
Paolo Bisegna
Keyword(s):  
Cell Mechanics ◽  
Cell Biology ◽  
Single Cell Level ◽  
Mechanical Function ◽  
Label Free ◽  
Cell Level ◽  
Lab On Chip ◽  
Non Invasive ◽  
Chip Technology ◽  
On Chip

Cell mechanics is a discipline that bridges cell biology with mechanics. Emerging microscale technologies are opening new venues in the field, due to their costeffectiveness, relatively easy fabrication, and high throughput. Two examples of those technologies are discussed here: microfluidic impedance cytometry and erythrocyte electrodeformation. The former is a lab-on-chip technology offering a simple, non-invasive, label-free method for counting, identifying and monitoring cellular biophysical and mechanical function at the single-cell level. The latter is a useful complement to the former, enabling cell deformation under the influence of an applied electric field.

Marker-free detection of progenitor cell differentiation by analysis of Brownian motion in micro-wells

2015 ◽  
Vol 7 (2) ◽  
pp. 178-183 ◽  
Author(s):  
Farzad Sekhavati ◽  
Max Endele ◽  
Susanne Rappl ◽  
Anna-Kristina Marel ◽  
Timm Schroeder ◽  
...  
Keyword(s):  
Brownian Motion ◽  
Cell Differentiation ◽  
Single Cell ◽  
High Throughput ◽  
Progenitor Cell ◽  
Single Cell Level ◽  
Label Free ◽  
Cell Level ◽  
Marker Free

The analysis of Brownian motion is a sensitive and robust tool for a label-free high-throughput investigation of cell differentiation at the single-cell level.

scholarly journals Environmental Monitoring of Pesticides by Immunoanalytical Techniques: Validation, Current Status, and Future Perspectives

2001 ◽  
Vol 84 (5) ◽  
pp. 1393-1406 ◽  
Author(s):  
Nanju A Lee ◽  
Ivan R Kennedy
Keyword(s):  
Risk Management ◽  
Liquid Chromatography ◽  
Environmental Monitoring ◽  
Environmental Contaminants ◽  
Analytical Tool ◽  
Current Status ◽  
Gas Liquid Chromatography ◽  
Future Perspectives ◽  
Reliable Monitoring ◽  
Recent Developments

Abstract Reliable monitoring technology is an essential component of effective regulation and risk management of environmental contaminants such as pesticides. Most environmental monitoring and analysis is currently conducted using instrumental techniques such as gas–liquid chromatography (GLC) and liquid chromatography (LC). Immunoanalysis provides powerful monitoring techniques that have emerged in the last 3 decades. This paper shows they can deliver rapid, accurate, and relatively inexpensive analysis with high throughput and that have the capability to be field oriented. The technique is versatile in application and can be formatted to suit different purposes such as quantitative analysis or simple “yes/no” tests that are field-portable. While there is a range of opinion on the merits of immunoassays as an analytical tool for pesticides, we suggest that this technology is best considered as complementary to GLC and LC, extending the range of capability for field monitoring. Supporting this view, an increasing number of successful applications of immunoassays to monitoring have been reported in recent years. We also report here the implications of recent developments in the field of immunodiagnostics and their application to monitoring of environmental contaminants. We emphasise that, together with adequate validation by instrumental techniques, immunoassays provide monitoring services yielding realistic and comprehensive data for risk management, allowing decisions on appropriate action by various authorities to be made.

SPR Imaging for High Throughput, Label-Free Interaction Analysis

2009 ◽  
Vol 12 (8) ◽  
pp. 741-751 ◽  
Author(s):  
Christopher Lausted ◽  
Zhiyuan Hu ◽  
Leroy Hood ◽  
Charles Campbell
Keyword(s):  
High Throughput ◽  
Interaction Analysis ◽  
Label Free ◽  
Spr Imaging ◽  
Free Interaction

Schottky diodes based on 2D materials for environmental gas monitoring: a review on emerging trends, recent developments and future perspectives

2021 ◽  
Author(s):  
Minu Mathew ◽  
Chandra Sekhar Rout
Keyword(s):  
Gas Sensing ◽  
2D Materials ◽  
Schottky Diodes ◽  
High Sensitivity ◽  
Future Perspectives ◽  
Working Temperature ◽  
Emerging Trends ◽  
Gas Sensing Properties ◽  
Recent Developments ◽  
Sensitivity And Selectivity

This review details the fundamentals, working principles and recent developments of Schottky junctions based on 2D materials to emphasize their improved gas sensing properties including low working temperature, high sensitivity, and selectivity.

Sign in / Sign up

Export Citation Format

Share Document