Pathological Study of Blood Parasites in Rice Field Frogs,Hoplobatrachus rugulosus(Wiegmann, 1834)

One hundred and forty adult rice field frogs,Hoplobatrachus rugulosus(Wiegmann, 1834), were collected in Srakaew province, Thailand. For blood parasite examination, thin blood smears were made and routinely stained with Giemsa. The results showed that 70% of the frogs (98/140) were infected with 5 species of blood parasites, including aTrypanosoma rotatorium-like organism,Trypanosoma chattoni, Hepatozoonsp. a,Hepatozoonsp. b, andLankesterella minima. Pathological examination of the liver, lung, spleen, and kidney of the frogs that were apparently infected with one of these blood parasites were collected and processed by routine histology and subsequently stained with haematoxylin and eosin. Histopathological findings associated with theTrypanosoma rotatorium-like organism andTrypanosoma chattoni-infected frogs showed no pathological lesions.Hepatozoonsp. a andHepatozoonsp. b-infected frogs developed inflammatory lesions predominantly in the liver, demonstrating granuloma-like lesions withHepatozoonsp. meronts at the centre. Tissue sections ofLankesterella minima-infected frogs also showed lesions. Liver and spleen showed inflammatory lesions with an accumulation of melanomacrophage centres (MMCs) surrounding the meronts and merozoites. It is suggested thatHepatozoonsp. a,Hepatozoonsp. b, andLankesterella minima-infections are capable of producing inflammatory lesions in the visceral organs of rice field frogs, and the severity of lesions is tentatively related to levels of parasitemia.

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Blood parasites in passerine birds from the Brazilian Atlantic Forest

Revista Brasileira de Parasitologia Veterinária ◽

10.1590/s1984-29612012000100003 ◽

2012 ◽

Vol 21 (1) ◽

pp. 7-15 ◽

Cited By ~ 15

Author(s):

Fabiane Sebaio ◽

Érika Martins Braga ◽

Felipe Branquinho ◽

Alan Fecchio ◽

Miguel Ângelo Marini

Keyword(s):

Atlantic Forest ◽

Bird Species ◽

Parasite Prevalence ◽

Neotropical Region ◽

Blood Parasite ◽

Blood Parasites ◽

Passerine Birds ◽

Blood Smears ◽

Temporal And Spatial ◽

Low Prevalence

Parasites may lead bird species to extinction, affect host temporal and spatial population dynamics, alter community structure and alter individuals’ social status. We evaluated blood parasite prevalence and intensity according to bird families and species, among 925 birds that were caught in 2000 and 2001, in the Atlantic Forest in the State of Minas Gerais, Brazil. We applied Giemsa staining to thin blood smears, to detect blood parasites. The birds (n = 15.8%) in 11 families, were infected by at least one parasite genus, especially Muscicapidae (28.3%) and Conopophagidae (25%). Among the 146 infected birds, Plasmodium was detected in all bird families and had the highest prevalence (54.8%). Trypanosoma, Haemoproteus and microfilaria had lower prevalence rates (23.3, 23.3 and 2.1%, respectively). Birds caught during the rainy season were more infected than birds caught during the dry season. The overall low prevalence of blood parasites in birds is similar to the patterns found elsewhere in the Neotropical region.

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Blood parasites of fish from the northwestern Atlantic Ocean

Canadian Journal of Zoology ◽

10.1139/z80-110 ◽

1980 ◽

Vol 58 (5) ◽

pp. 770-781 ◽

Cited By ~ 27

Author(s):

R. A. Khan ◽

M. Barrett ◽

J. Murphy

Keyword(s):

Atlantic Ocean ◽

Marine Fish ◽

Cold Water ◽

The Other ◽

Blood Parasites ◽

Scotian Shelf ◽

Blood Smears ◽

Water Species ◽

Cold Water Species ◽

Thin Blood Smears

Examination of thick and thin blood smears from 5013 marine fish from the northwestern Atlantic Ocean revealed 25 of 59 species to be infected with one or more of the following blood parasites: trypanosomes (27% of 3610), trypanoplasms (9% of 588), piroplasms (26% of 2584), and haemogregarines (36% of 1708). Higher prevalences of infection were observed in cold-water, benthic, sedentary fish than in warm-water, littoral, epipelagic, or midwater species. Trypanosome infections were more prevalent among fish taken off the Labrador – east Newfoundland coasts, lower in those from St. Pierre Bank, Grand Bank, and Gulf of St. Lawrence, and lowest in forms from the Scotian Shelf. Seven of nine species of hematophagous leeches harboured asexual stages of haemogregarines, whereas natural infections of trypanosomes occurred in one leech, Johanssonia arctica. Using five species of laboratory-reared leeches and laboratory-initiated infections of trypanosomes in piscine hosts, development of Trypanosoma murmanensis was observed only in J. arctica. It is suggested that the distribution of trypanosomes of marine fish is related to that of J. arctica, a cold-water species, whereas the other haematozoan infections might be attributed to those leech species that are associated with their respective piscine hosts.

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Detection Of Blood Parasites In Cattle In Kutalimbaru Subdistrict, Deli Serdang Regency, North Sumatera

E3S Web of Conferences ◽

10.1051/e3sconf/202015101040 ◽

2020 ◽

Vol 151 ◽

pp. 01040

Author(s):

Mudhita Z. Ritonga ◽

Andhika Putra ◽

Agun Prastia ◽

Firdaus Nasution ◽

Risdawati Br Ginting

Keyword(s):

Animal Species ◽

Staining Technique ◽

Diagnostic Techniques ◽

Blood Parasite ◽

Control Measures ◽

Blood Parasites ◽

Susceptible Animal ◽

Healthy Cattle ◽

Blood Smears

The aim of this research was to identify and to find out the prevalence of blood parasites in cattle in Kutalimbaru Subdistrict, Deli Serdang Regency. This research was conducted in Kutalimbaru Subdistrict, Deli Serdang Regency from December 2018 to February 2019. Normal/healthy cattle of different breeds were randomly selected from five locations, namely Sei Mencirim village, Sawit Rejo village, Silebo-lebo village, Sampe Cita village and Pasar X village. A total of 150 blood samples were collected for blood smears. Diagnostic techniques were tested using Giemsa's staining technique. The blood parasites were then identified and their prevalence was determined. The results showed that one species of blood parasite was successfully identified, all belonging to the blood parasite, namely, Theileria sp. with a prevalence of 33%. In addition, there were no species of blood parasite from the Anaplasma, Trypanosoma, and Babesia. This study suggests that theileriosis is spreading to even the other region in North Sumatera. To reduce the infection, follow up surveys of the blood parasites of the livestock, assess their distribution and infection rates of possible vectors are recommended and plans control measures against should be focused on reducing transmission to highly susceptible animal species.

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Occurrence of common blood parasites of cattle in Sirajgonj sadar area of Bangladesh

Bangladesh Journal of Veterinary Medicine ◽

10.3329/bjvm.v4i2.1300 ◽

1970 ◽

Vol 4 (2) ◽

pp. 143-145 ◽

Cited By ~ 5

Author(s):

S Chowdhury ◽

MA Hossain ◽

SR Barua ◽

S Islam

Keyword(s):

Key Words ◽

Peripheral Blood ◽

Blood Parasite ◽

Blood Parasites ◽

Epidemiological Investigation ◽

Blood Smears ◽

Protozoal Infection ◽

Peripheral Blood Smears ◽

Veterinary Hospital ◽

Anaplasma Centrale

Â An epidemiological investigation was carried out on common blood parasites (protozoa and rickettsia) in clinically suspected (febrile, anorectic, non responsive to antibiotics therapy) cattle attended at District veterinary hospital of Sirajgong. Total 60 cattle were examined for blood parasite (protozoa and rickettsia) infection. Giemsa's stained were used on peripheral blood smears of suspected cattle. These slides were examined microscopically during September to October, 2004, of which 42 cattle (70 % of the total examined cattle) had Anaplasma infection (either Anaplasma marginale or Anaplasma centrale or both) and 2 cattle (3.3% of the total cattle) had Babesia infection but negative for other blood protozoal infection. Key words: Anaplasmosis, babesiosis, cattle, occurrence DOI = 10.3329/bjvm.v4i2.1300 Bangl. J. Vet. Med. (2006). 4 (2): 143-145

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Absence of haemoparasite infection in the fossorial amphisbaenian Trogonophis wiegmanni

Parasitology ◽

10.1017/s0031182016000950 ◽

2016 ◽

Vol 143 (11) ◽

pp. 1433-1436

Author(s):

JOSÉ MARTÍN ◽

MARIO GARRIDO ◽

JESÚS ORTEGA ◽

ROBERTO GARCÍA-ROA ◽

ALEJANDRO IBÁÑEZ ◽

...

Keyword(s):

Parasite Infection ◽

Blood Parasite ◽

Blood Parasites ◽

Lizard Species ◽

North West ◽

Ecological Adaptations ◽

Blood Smears ◽

Distinctive Group ◽

Almost All ◽

Host Parasite

SUMMARYBlood parasites such as haemogregarines and haemosporidians have been identified in almost all groups of vertebrates. However, very little is known about biodiversity of these parasites and their effects on some major groups of reptiles such as amphisbaenians, a distinctive group with many morphological and ecological adaptations to fossorial life. Conditions of the fossorial environment might also affect host–parasite relationships. We investigated the presence and the potential prevalence of three genera of haemoparasitic aplicomplexan blood parasites (Hepatozoon, Plasmodium and Haemoproteus) in the amphisbaenian Trogonophis wiegmanni, a fossorial worm lizard species from North West Africa. Blood parasite infection was not detected in T. wiegmanni, both in visual surveys of blood smears and using molecular methods to detect DNA of such parasites in the blood of the potential amphisbaenian hosts. We discuss how conditions of the fossorial environment might affect blood parasitaemias in amphisbaenians as well as in other fossorial reptiles.

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Brightfield and fluorescence in-channel staining of thin blood smears generated in a pumpless microfluidic

Analytical Methods ◽

10.1039/d1ay00195g ◽

2021 ◽

Author(s):

Kokou S. Dogbevi ◽

Paul Gordon ◽

Kimberly L. Branan ◽

Bryan Khai D. Ngo ◽

Kevin B. Kiefer ◽

...

Keyword(s):

Peripheral Blood ◽

Blood Smears ◽

Peripheral Blood Smears ◽

Thin Blood Smears

Effective staining of peripheral blood smears which enhances the contrast of intracellular components and biomarkers is essential for the accurate characterization, diagnosis, and monitoring of various diseases such as malaria.

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Pathological Study of Stillbirths Placentas at Muhimbili National Hospital, Tanzania

American Journal of Clinical Pathology ◽

10.1093/ajcp/aqaa161.041 ◽

2020 ◽

Vol 154 (Supplement_1) ◽

pp. S22-S23

Author(s):

A E Malango

Keyword(s):

Clinical Diagnosis ◽

Clinical Assessment ◽

Gestational Age ◽

Fetal Death ◽

Consensus Statement ◽

Cross Sectional Study ◽

Pathological Examination ◽

Pathological Study ◽

Cross Sectional ◽

Placenta Pathology

Abstract Introduction/Objective Stillbirth is defined as fetal death that occurs at gestational age of ≥28 weeks. In our setting clinical assessment is the only method used to determine cause of stillbirths, with no reported proportion of unknown clinical diagnosis. Studies showed that unknown cause of stillbirths can be reduced by examination of placenta. Causal identification aids in the mourning process and identifying recurrence risks. The study aimed to describe pathological changes in the placentas of stillbirths which have risk to cause fetal death Methods A descriptive cross-sectional study done for the period of 6 months, it involved examination of 80 placentas of stillbirths born at gestational age of ≥ 28 weeks, placentas were fixed in 10% neutral buffered formalin for 8–12 hours. Grossing and interpretation of placenta pathology was according to Amsterdam Placental Workshop Group Consensus Statement. Results Out of 80 stillbirths, 32(40%) had unknown clinical diagnosis. Majority of stillbirth placentas 71(91%) found with either one or combined pathologies with the risk to cause stillbirth. Maternal vascular malperfusion was the commonest pathology and was significantly associated with preterm stillbirths. Maternal floor infarction, a placenta pathology with risk to cause fetal death and high risk of recurrence was among the pathologies found, was seen in 4(5%) of stillbirth placentas. Conclusion Findings in this study clearly indicated the importance of pathological examination of placenta in determining cause of stillbirth. Placenta examination in stillbirths can identify more pathology related to stillbirths than clinical assessment alone.

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Interactions between the intestinal flagellates Giardia muris and Spironucleus muris and the blood parasites Babesia microti, Plasmodium yoelii and Plasmodium berghei in mice

Parasitology ◽

10.1017/s0031182000054184 ◽

1982 ◽

Vol 85 (1) ◽

pp. 101-110 ◽

Cited By ~ 2

Author(s):

Sara J. Brett ◽

F. E. G. Cox

Keyword(s):

Small Intestine ◽

Total Duration ◽

Plasmodium Yoelii ◽

Blood Parasite ◽

Blood Parasites ◽

Babesia Microti ◽

Parasite Infections ◽

Immunological Factors ◽

Temporary Decrease ◽

Giardia Muris

SUMMARYIn mice infected with the intestinal flagellates Giardia muris or Spironucleus muris, together with the blood parasites Babesia microti or Plasmodium yoelii, there is a temporary decrease of flagellate cyst output coincident with the peak of the blood parasite infections, followed by a rapid return to normal levels. This decrease in cyst output is correlated with decreased numbers of trophozoites in the small intestine. The effect on S. muris is more marked than that on G. muris. Neither blood parasites has any effect on the total duration of the flagellate infection and the flagellates do not affect the blood parasites. In mice infected with G. muris or S. muris and P. berghei there is also a decrease in cyst output but this is less apparent than in infections with B. microti or P. yoelii because of the fatal nature of the P. berghei infection. It is suggested that the decrease in cyst output is probably due to changes in the contents of the small intestine or to non-specific immunological factors rather than to specific immunological changes.

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