Batu (Musa balbisiana Colla) and Kepok (Musa paradisiaca formatypica) bananas are
two food commodities with high resistant starch content. The later has been suggested to
prevent colorectal cancer. Modifications of these, into flour via the addition of pullulanase
enzymes and autoclaving-cooling method are expected to improve the quality. The
physical characteristics, levels of resistant starch, amylose, amylopectin and in vitro
digestibility of enzymatically modified banana starches were determined. Therefore, a
descriptive study was performed with untreated Batu banana flour (TP), autoclavingcooling (AC), autoclaving-cooling + pullulanase enzyme (AC + E), and autoclavingcooling + pullulanase enzyme + autoclaving-cooling (AC + E + AC). The granular
morphology, water absorption, and swelling ability were evaluated as physical
characteristics. Amylose, amylopectin content, and starch digestibility were evaluated as
chemical characteristics. Based on SEM, the analysis of granular morphology showed
structural modification in both treatments, although Batu banana flour showed greater
water absorption and swelling ability, with and without AC + E + AC. However, both
samples demonstrated better outcomes, in terms of high levels of starch resistance, low
amylose, high amylopectin, and low in vitro digestibility after treatment with AC + E +
AC.